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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | A Predictive Model for Lymph Node Yield in Colon Cancer Resection Specimens显示文摘 | Garrett M. Nash David Row Alexander Weiss Jinru Shia Jose G. Guillem Philip B. Paty Mithat Gonen Martin R. Weiser Larissa K. Temple Garrett Fitzmaurice W. Douglas Wong | 2011 | Annals of Surgery2011，，2： | 2 |
| 3 | Predictors for Negative Ureteroscopy in the Management of Upper Urinary Tract Stone Disease显示文摘 | Jessica E. Kreshover Rian J. Dickstein Courtney Rowe Richard K. Babayan David S. Wang | 2011 | Urology2011，，4： | 2 |
| 4 | Basel II and Economic Capital显示文摘 | David Rowe Dean Jovic Richard Reeves | 2004 | US BANKER2004，，72： | 1 |
| 5 | Aroma chemicals for savory flavors 显示文摘 | David J Rowe | 1998 | Perfumery & Flavorist1998，23，4： | 1 |
| 6 | 食用菌与人类健康(一)显示文摘2013年9月9~10日,美国蘑菇理事会在华盛顿召开食用菌与健康峰会。会议指出,将食用菌定位为健康食品和养生保健品的研究主要集中于最近的几十年。中国是最早栽培食用菌的国家,目前仍然和美国、意大利、荷兰、波兰一样,是全球食用菌生产大国之一。虽然在饮食上,食用菌被认为是一种蔬菜,但它是一种真菌,区别在于食用菌中含量极低的维生素B12的存在形式与肉类的相同,麦角固醇能通过紫外光照转换为维生素D2和共轭亚油酸。食用菌是麦角硫因(存在于自然界的一种稀有氨基酸,在机体内具有重要的生理活性)的罕见来源,同时含有硒、纤维素及其他维生素和矿物质。一些临床前和临床试验,研究了食用菌对认知能力、体重管理、口腔健康和患癌几率的影响。初步证据表明,食用菌可能通过与肠道菌群的互动,提供良好的免疫和炎症反应,增强适应性免疫的发展和改进免疫细胞功能。除了直接提供营养和健康福祉之外,美国食物摄入量调查数据的分析表明,食用菌还与更高的膳食质量有关。早期的感官研究表明,食用菌与肉类和低钠菜肴混合很受欢迎,在不影响口味的情况下可能有助于减少红色肉类(牛、羊肉等)和盐的摄入。食用菌特定的健康效应研究进展表明,需要通过有效的沟通交流,实现利用食用菌来提高整体饮食质量的目标。. | Mary Jo Feeney Johanna Dwyer Clare M.Hasler-Lewis John A.Milner Manny Noakes Sylvia Rowe Mark Wach Robert B.Beelman Joe Caldwell Margherita T.Cantorna Lisa A.Castlebury Shu-Ting Chang Lawrence J.Cheskin Roger Clemens Greg Drescher Victor L.Fulgoni Ⅲ David B.Haytowitz Van S.Hubbard David Law Amy Myrdal Miller Bart Minor Susan S.Percival Gabriela Riscuta Barbara Schneeman Suzanne Thornsbury Cheryl D.Toner Catherine E.Woteki Dayong Wu 郑玲 | 2015 | 食药用菌2015，23，2： | 1 |
| 7 | Sport and the repudiation of the global显示文摘 | David Rowe | 2003 | International Review for the Sociology of Sport2003，38，3： | 1 |
| 8 | Development of the Scottish Walkability Assessment Tool (SWAT)显示文摘 | Catherine Millington Catharine Ward Thompson David Rowe Peter Aspinall Claire Fitzsimons Norah Nelson Nanette Mutrie | | 0，，： | 1 |
| 9 | Increased Risk of Cognitive Impairment in Patients With Diabetes Is Associated With Metformin显示文摘 | Eileen M. Moore Alastair G. Mander David Ames Mark A. Kotowicz Ross P. Carne Henry Brodaty Michael Woodward Karyn Boundy Kathryn A. Ellis Ashley I. Bush Noel G. Faux Ralph Martins Cassandra Szoeke Christopher Rowe David A. Watters | 2013 | Diabetes Care2013，，10： | 1 |
| 10 | Protein kinase C activation antagonizes melatonin-induced pigment aggregation in xenopus laevis melanophores显示文摘 | David Sugden Sally J Rowe | 1992 | J Cell Biol1992，119，6： | 1 |
| 11 | Accommodating Bodies:Celebrity,Sexuality,and 'Tragic Magic'显示文摘 | David Rowe | 1994 | Journal of Sport & Social Issues1994，18，1： | 1 |
| 12 | Initial Demonstration of in Vivo Tracing of Axonal Projections in the Macaque Brain and Comparison with the Human Brain Using Diffusion Tensor Imaging and Fast Marching Tractography显示文摘 | Geoffrey J.M. Parker Klaas E. Stephan Gareth J. Barker James B. Rowe David G. MacManus Claudia A.M. Wheeler-Kingshott Olga Ciccarelli Richard E. Passingham Rachel L. Spinks Roger N. Lemon Robert Turner | 2002 | Neuroimage2002，，4： | 1 |
| 13 | Sport in Malaysia: National Im- peratives and Western Seductions 显示文摘 | Callum Gilmour David Rowe | 2012 | Sociology of Sport Jour- nal2012，29，48： | 1 |
| 14 | PGC-1[alpha] promotes recovery after acute kidney injury during systemic inflammation in mice显示文摘 | Tran Mei Tam Denise Bardia Amit Bhasin Manoj Rowe Glenn C Kher Ajay Zsengeller Zsuzsanna K Akhavan-Sharif M Reza Khankin Eliyahu V Saintgeniez Magali David Sascha Burstein Deborah Karumanchi S Ananth Stillman Isaac E Arany Zoltan Parikh Sa | 2011 | Journal of Clinical Investigation2011，，10： | 1 |
| 15 | Sclerostin is a locally acting regulator of late‐osteoblast/preosteocyte differentiation and regulates mineralization through a MEPE‐ASARM‐dependent mechanism显示文摘 | Gerald J Atkins Peter S Rowe Hui P Lim Katie J Welldon Renee Ormsby Asiri R Wijenayaka Lesya Zelenchuk Andreas Evdokiou David M Findlay | 2011 | J Bone Miner Res2011，，7： | 1 |
| 16 | A Rapid Method to Score Stream Reaches Based on the Overall Performance of Their Main Ecological Functions显示文摘 | David K Rowe S P John Q | 2009 | Environmental Management2009，，43： | 1 |
| 17 | An Elemental Diet Containing Medium-Chain Triglycerides and Enzymatically Hydrolyzed Protein can Improve Gastrointestinal Tolerance in People Infected with HIV显示文摘 | SHARON B SALOMON JOE JUNG THERESA VOSS ANNE SUGUITAN W BRUCE ROWE DAVID C MADSEN | 1998 | Journal of the American Dietetic Association1998，，： | 1 |
| 18 | If you film it, Will they come? Sports on Film 显示文摘 | David Rowe | 1998 | Journal of Sport and Social Issues1998，22，4： | 1 |
| 19 | Sport and the Repudiation of the Global显示文摘 | DAVID ROWE | 2003 | Intl Rev Sociology Sport2003，38，3： | 1 |
| 20 | Using Cadence to Study Free-Living Ambulatory Behaviour显示文摘 | Catrine Tudor-Locke David A. Rowe | 2012 | Sports Medicine2012，，： | 1 |
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