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7篇 您的检索式：作者名="David J Rowe"

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		题名	作者	年代	出处	被引量
	1	Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds.	Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes	2017	World Journal of Gastroenterology2017，23，2：	6
	2	Aroma chemicals for savory flavors 显示文摘	David J Rowe	1998	Perfumery & Flavorist1998，23，4：	1
	3	Protein kinase C activation antagonizes melatonin-induced pigment aggregation in xenopus laevis melanophores显示文摘	David Sugden Sally J Rowe	1992	J Cell Biol1992，119，6：	1
	4	Sclerostin is a locally acting regulator of late‐osteoblast/preosteocyte differentiation and regulates mineralization through a MEPE‐ASARM‐dependent mechanism显示文摘	Gerald J Atkins Peter S Rowe Hui P Lim Katie J Welldon Renee Ormsby Asiri R Wijenayaka Lesya Zelenchuk Andreas Evdokiou David M Findlay	2011	J Bone Miner Res2011，，7：	1
	5	Aroma chemicals for savory flavors显示文摘	David J Rowe	1998	Perfumer &flavorist1998，23，4：	1
	6	A distinct lineage of influenza A virus from bats 显示文摘	Suxiang Tong Yah Li Pierre Rivailler Christina Con- rardy Danilo A Alvarez Castillo Li-Mei Chen Sergio Recuenco James A Ellison Charles T Davis Ian A York Amy S Turmelle David Moran Shannon Rog- ers Mang Shi Ying Tao Michael R Weil Kevin Tang Lori A Rowe Scott Sammons Xiyan Xu Mi- chael Frace Kim A Lindblade Nancy J Cox Larry J Anderson Charles E Rupprecht Ruben O Donis	2012	PANS U S A2012，109，11：	1
	7	Aroma chemicals for savory flavors显示文摘	David J Rowe	1998	Perfumer & Flavorist1998，23，4：	1

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