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| 1 | Toward point-of-care assessment of patient response:a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning显示文摘We present a novel method to rapidly assess drug efficacy in targeted cancer therapy,where antineoplastic agents are conjugated to antibodies targeting surface markers on tumor cells.We have fabricated and characterized a device capable of rapidly assessing tumor cell sensitivity to drugs using multifrequency impedance spectroscopy in combination with supervised machine learning for enhanced classification accuracy.Currently commercially available devices for the automated analysis of cell viability are based on staining,which fundamentally limits the subsequent characterization of these cells as well as downstream molecular analysis.Our approach requires as little as 20μL of volume and avoids staining allowing for further downstream molecular analysis.To the best of our knowledge,this manuscript presents the first comprehensive attempt to using high-dimensional data and supervised machine learning,particularly phase change spectra obtained from multi-frequency impedance cytometry as features for the support vector machine classifier,to assess viability of cells without staining or labelling. | Karan Ahuja Gulam M.Rather Zhongtian Lin Jianye Sui Pengfei Xie Tuan Le Joseph R.Bertino Mehdi Javanmard | 2019 | Microsystems & Nanoengineering2019，5，1： | 4 |
| 2 | Diuretic and antioxidant activities of the aqueous extract of leaves of Cassia occidentalis(Linn.) in rats显示文摘Objective: To assess the putative diuretic and antioxidant properties of Cassia occidentalis(C. occidentalis) leaves' aqueous extract. Methods: Adult rats were administered with C. occidentalis leaves aqueous extract acutely(24-h) and subchronically(7 d), at doses 80, 160, 240, 320, and 400 mg/kg(per os). Negative control group received only an equivalent volume of distilled water, while the two positive control groups received the diuretic drugs furosemide(20 mg/kg, i.p.) and hydrochlorothiazide(HCTZ, 20 mg/kg, i.p.). Urinary elimination of electrolytes in response to treatments was evaluated, together with changes in concentrations of creatinine, urea, aldosterone, glucose, and albumin in urine and plasma. Various urinary indicators of kidney function and plasmatic markers of oxidative stress were also assessed. Results: The acute administration of C. occidentalis increased the urinary excretion of 107.58% at the higher dose tested, compared to negative control. The reference drugs furosemide and HCTZ induced increases of 84.27 % and 48.05 %, respectively. Acutely, the extract induced Na+ and Cl- elimination, whereas subchronically an increase in K+ elimination was also observed. The extract also improved the kidney function indexes and oxidative stress markers. These effects were dose-dependent and comparable with positive control observations. Conclusions: Our i ndings strongly suggest that C. occidentalis aqueous extract has diuretic and antioxidant activities, and deserves further studies considering the potential for the treatment of hypertension. | Théophile Dimo Fidèle Ntchapda Joseph Barama David Romain Kemeta Azambou Paul Faustin Seke Etet | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，9： | 2 |
| 3 | A Lethally Irradiated Allogeneic Granulocyte-Macrophage Colony Stimulating Factor-Secreting Tumor Vaccine for Pancreatic Adenocarcinoma: A Phase II Trial of Safety, Efficacy, and Immune Activation显示文摘 | Lutz Eric Charles J. Yeo Keith D. Lillemoe Barbara Biedrzycki Barry Kobrin Joseph Herman Elizabeth Sugar Steven Piantadosi John L. Cameron Sara Solt Beth Onners Irena Tartakovsky Miri Choi Rajni Sharma Peter B. Illei Hruban Ralph H. Ross A. Abrams Dung Le | 2011 | Annals of Surgery2011，，2： | 2 |
| 4 | Current evidence on potential of adipose derived stem cells to enhance bone regeneration and future projection显示文摘Injuries to the postnatal skeleton are naturally repaired through successive stepsinvolving specific cell types in a process collectively termed “bone regeneration”.Although complex, bone regeneration occurs through a series of well-orchestratedstages wherein endogenous bone stem cells play a central role. In most situations,bone regeneration is successful;however, there are instances when it fails andcreates non-healing injuries or fracture nonunion requiring surgical or therapeuticinterventions. Transplantation of adult or mesenchymal stem cells (MSCs) definedby the International Society for Cell and Gene Therapy (ISCT) as CD105+-CD90+CD73+CD45-CD34-CD14orCD11b-CD79αorCD19-HLA-DR- is beinginvestigated as an attractive therapy for bone regeneration throughout the world.MSCs isolated from adipose tissue, adipose-derived stem cells (ADSCs), aregaining increasing attention since this is the most abundant source of adult stemcells and the isolation process for ADSCs is straightforward. Currently, there isnot a single Food and Drug Administration (FDA) approved ADSCs product forbone regeneration. Although the safety of ADSCs is established from their usagein numerous clinical trials, the bone-forming potential of ADSCs and MSCs, ingeneral, is highly controversial. Growing evidence suggests that the ISCT definedphenotype may not represent bona fide osteoprogenitors. Transplantation of bothADSCs and the CD105- sub-population of ADSCs has been reported to induce bone regeneration. Most notably, cells expressing other markers such as CD146,AlphaV, CD200, PDPN, CD164, CXCR4, and PDGFRα have been shown torepresent osteogenic sub-population within ADSCs. Amongst other strategies toimprove the bone-forming ability of ADSCs, modulation of VEGF, TGF-β1 andBMP signaling pathways of ADSCs has shown promising results. The U.S. FDAreveals that 73% of Investigational New Drug applications for stem cell-basedproducts rely on CD105 expression as the “positive” marker for adult stem cells.A concerted effort involving the scientific community, clinicians, industries, andregulatory bodies to redefine ADSCs using powerful selection markers andstrategies to modulate signaling pathways of ADSCs will speed up thetherapeutic use of ADSCs for bone regeneration. | Quang Le Vedavathi Madhu Joseph M Hart Charles R Farber Eli R Zunder Abhijit S Dighe Quanjun Cui | 2021 | World Journal of Stem Cells2021，13，9： | 2 |
| 5 | In Vivo and In Vitro Pharmacological Studies of Methoxycarbonyl-Carboetomidate显示文摘 | Ervin Pejo Joseph F. Cotten Elizabeth W. Kelly Ri Le Ge Gregory D. Cuny Joydev K. Laha Jifeng Liu Xiang Jie Lin Douglas E. Raines | 2012 | Anesthesia & Analgesia2012，，： | 2 |
| 6 | Effects of chloride and sulfate on the rate of oxidation of ferrous ion by H 2 O 2显示文摘 | Giang Le Truong Joseph De Laat Bernard Legube | 2004 | Water Research2004，，9： | 1 |
| 7 | Analysis of fiducials implanted during EUS for patients with localized rectal cancer receiving high-dose rate endorectal brachytherapy显示文摘 | Shalini Moningi Amanda J. Walker Ashkan A. Malayeri Lauren M. Rosati Susan Gearhart Jonathan Efron Elizabeth Wick Nilofer Azad Elwood P. Armour Yi Le Joseph M. Herman Eun Ji Shin | 2014 | Gastrointestinal Endoscopy2014，，： | 1 |
| 8 | Patient reported outcome measures following specialistnurse - led clinics in preparation for breast reconstructionsurger显示文摘 | Caroline LE Osbome Fiona G Court Joseph MO Donoghue | 2010 | The Breast2010，19，： | 1 |
| 9 | Mechanical energy transductions in standing wave ultrasonic motors 显示文摘 | Patrice Le Moal Eric Joseph Jean-Claude Ferniot | 2000 | Analytical modelling and experimental investigations2000，19，5： | 1 |
| 10 | Oxidative stress and stress-activated signaling pathways:a unifying hypothesis of type 2 diabetes显示文摘 | Joseph LE Ira DG Betty AM | 2002 | Endoc Rev2002，23，5： | 1 |
| 11 | Vitamin C as effective as combinations of antioxidant nutrients in reducing sympotoms of upper respiratory tract infection in ultramarathom runners显示文摘 | Peters EM Goetzsche JM Joseph LE et aI | 1996 | S Afr Sports Med1996，11，3： | 1 |
| 12 | Oxidative stress and stressactivated signaling pathways: A unifying hypothesis of type 2 diabetes显示文摘 | Joseph LE Ira DG Betty AM | 2002 | Endocr Rev2002，23，5： | 1 |
| 13 | Implanted adipose progenitor cells as physicochemical regulators of breast cancer显示文摘 | Emily M. Chandler Bo Ri Seo Joseph P. Califano Roberto C. Andresen Eguiluz Jason S. Lee Christine J. Yoon David T. Tims James X. Wang Le Cheng Sunish Mohanan Mark R. Buckley Itai Cohen Alexander Yu Nikitin Rebecca M. Williams Delphine Gourdon Cynthia A. R | 2012 | Proceedings of the National Academy of Sciences2012，，25： | 1 |
| 14 | Preliminary results of the initial United States experience with the Supera woven nitinol stent in the popliteal artery显示文摘 | Luis R. León Robert S. Dieter Crystal L. Gadd Erika Ranellone Joseph L. Mills Miguel F. Montero-Baker Angelika C. Gruessner John P. Pacanowski | 2013 | Journal of Vascular Surgery2013，，： | 1 |
| 15 | Combined effect of low frequency ultrasound and iontophoresis: applications for transdermal heparin delivery显示文摘 | Long Le Joseph Kost Samir Mitragotri | 2000 | Pharm Res2000，17，： | 1 |
| 16 | Preliminary results of the initial United States experience with the Supera woven nitinol stent in the popliteal artery显示文摘 | Luis R. León Robert S. Dieter Crystal L. Gadd Erika Ranellone Joseph L. Mills Miguel F. Montero-Baker Angelika C. Gruessner John P. Pacanowski | 2013 | Journal of Vascular Surgery2013，，4： | 1 |
| 17 | Glucose-Independent Glutamine Metabolism via TCA Cycling for Proliferation and Survival in B Cells显示文摘 | Anne Le Andrew N. Lane Max Hamaker Sminu Bose Arvin Gouw Joseph Barbi Takashi Tsukamoto Camilio J. Rojas Barbara S. Slusher Haixia Zhang Lisa J. Zimmerman Daniel C. Liebler Robbert J.C. Slebos Pawel K. Lorkiewicz Richard M. Higashi Teresa W.M. Fan Chi V. | 2012 | Cell Metabolism2012，，1： | 1 |
| 18 | Vitamin C supplementation reduces the Incidence of postrace symptoms of upper respiratory tract infection in ultramarathon runners 显示文摘 | Peters EM Goetzsche JM Joseph LE | 1993 | Am J Clin Nutr1993，57，： | 1 |
| 19 | Vitamin C is as effective as combinations of abti-oxidant nutrients in reducing symptoms of upper respiratory tract infection in ultramarathon runners显示文摘 | Peters EM Goetzsche JM Joseph LE | 1996 | S Afr Sportmed1996，3，： | 1 |
| 20 | Transplantation of kidneys from hypertensive cadaveric donors 显示文摘 | Ratner LE Joseph V Zibari G | 1995 | Transplant Proc1995，27，1： | 1 |
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