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| 1 | Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy显示文摘Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future. | Bin Liu Wen Cao Jin Cheng Sisi Fan Shaojun Pan Lirui Wang Jiaqi Niu Yunxiang Pan Yanlei Liu Xiyang Sun Lijun Ma Jie Song Jian Ni Daxiang Cui | 2019 | Cancer Biology & Medicine2019，16，4： | 7 |
| 2 | Estimation of the three-dimensional in situ stress field around a large deep underground cavern group near a valley显示文摘Understanding three-dimensional(3D)in situ stress field is of key importance for estimating the stability of large deep underground cavern groups near valleys.However,the complete 3D in situ stress fields around large deep underground cavern groups are difficult to determine based on in situ stress data from a limited number of measuring points due to the insufficient representativeness and unreliability of such measurements.In this study,an integrated approach for estimating the 3D in situ stress field around a large deep underground cavern group near a valley is developed based on incomplete in situ stress measurements and the stress-induced failures of tunnels excavated prior to the step excavation of the cavern group.This integrated approach is implemented via four interrelated and progressive basic steps,i.e.inference of the regional tectonic stress field direction,analyses of in situ stress characteristics and measurement reliability,regression-based in situ stress field analysis and reliability assessment,and modified in situ stress field analysis and reliability verification.The orientations and magnitudes of the 3D in situ stress field can be analyzed and obtained at a strategic level following these four basic steps.First,the tectonic stress field direction around the cavern group is deduced in accordance with the regional tectonic framework and verified using a regional crustal deformation velocity map.Second,the reliability of the in situ stress measurements is verified based on the locations and depths of stressinduced brittle failures in small tunnels(such as exploratory tunnels and pilot tunnels)within the excavation range of the cavern group.Third,considering the influences of the valley topography and major geological structures,the 3D in situ stress field is regressed using numerical simulation and multiple linear regression techniques based on the in situ stress measurements.Finally,the regressed in situ stress field is further modified and reverified based on the stress-induced brittle failures of small tunnels and the initial excavation of the cavern group.A case study of the Shuangjiangkou underground cavern group demonstrates that the proposed approach is reliable for estimating the 3D in situ stress fields of large deep underground cavern groups near valleys,thus contributing to the optimization of practical excavation and design of mitigating the instability of the surrounding rock masses during step excavations. | Dingping Xu Xiang Huang Quan Jiang Shaojun Li Hong Zheng Shili Qiu Huaisheng Xu Yonghong Li Zhiguo Li Xingdong Ma | 2021 | Journal of Rock Mechanics and Geotechnical Engineering2021，13，3： | 6 |
| 3 | GSH-triggered sequential catalysis for tumor imaging and eradication based on star-like Au/Pt enzyme carrier system显示文摘Distinctively different metabolism between tumor cells and normal cells endows tumor tissues unique microenvironment.In this regard,we have successfully prepared a sequential catalytic platform based on Au/Pt star for tumor theragnostic.The multifunctional probes consisted of a gold/platinum star-shaped core(Au/Pt star)conjugated with a GSH-sensitive disulfide bond(S–S),a targeting ligand(rHSA-FA),a near-infrared fluorophore(IR780)and glucose oxidase(GOx).When systemically administered in a xenografted murine model,the probes specifically targeted the tumor sites.As the disulfide linker was cleaved by intracellular GSH,the IR780 molecules could be released for photo-thermal therapy&photodynamic therapy(PTT&PDT)and imaging.Subsequently,the Pt nanolayer of the Au/Pt star and the GOx formed a sequential catalytic system:GOx effectively catalyzed intracellular glucose by consuming oxygen to generate H2O2 and enhance the local acidity,and the Pt layer exhibited peroxidase-like property to catalyze H2O2 producing toxic·OH for tumor oxidative damage.Here we demonstrated that our probes simultaneously possessed a GSH-sensitive release,real-time imaging ability,and synergetic cancer starving-like therapy/enzyme oxidative therapy/PTT/PDT features,which provides a potential strategy for effective tumor theragnostic. | Amin Zhang Qian Zhang Gabriel Alfranca Shaojun Pan Zhicheng Huang Jin Cheng Qiang Ma Jie Song Yunxiang Pan Jian Ni Lijun Ma Daxiang Cui | 2020 | Nano Research2020，13，1： | 4 |
| 4 | Natural variations in grain length 10(GL10)regulate rice grain size显示文摘Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characterized.Here,we describe the grain length QTL GL10,encoding MADS56,which positively regulates grain length and grain weight.A natural allelic variation of NIL-gl10,containing an~1.0-kb deletion in the first exon that abolishes its transcription,results in shorter grain length,lower grain weight and delayed flowering in gl10 plants.The knockout of GL10 in the HJX74 background leads to grain phenotypes similar to that of NIL-gl10,while overexpression of GL10 results in increased grain length and weight and earlier heading date.GL10 regulates grain length by promoting greater longitudinal cell growth in the grain glume.Additionally,GL10 participates in the regulation of gibberellic acid(GA)signaling pathway genes in young panicle tissues.Analysis of GL10 haplotypes shows obvious divergence between the japonica and indica lineages.Our findings reveal an allelic variation of GL10 that may explain differences in grain length among modern cultivars and could be used to breed rice varieties with optimized grain shape. | Penglin Zhan Shuaipeng Ma Zhili Xiao Fangping Li Xin Wei Shaojun Lin Xiaoling Wang Zhe Ji Yu Fu Jiahao Pan Mi Zhou Yue Liu Zengyuan Chang Lu Li Suhong Bu Zupei Liu Haitao Zhu Guifu Liu Guiquan Zhang Shaokui Wang | 2022 | Journal of Genetics and Genomics2022，49，5： | 3 |
| 5 | Clinical study of IL-18 and NANOG gene polymorphisms in prostate cancer patients显示文摘Objective Recent studies have shown abnormal expression of NANOG and IL-18 to be related to cancer. However, the molecular mechanism by which IL-18 and NANOG gene polymorphisms are associated with prostate cancer is unclear. In this study, we examined whether IL-18 and NANOG gene polymorphisms and their interaction with prostate cancer-related risk factor are associated with the susceptibility to and clinicopathological development of prostate cancer among Chinese men.Methods Polymorphisms in the NANOG and IL-18 genes were evaluated for susceptibility in 120 patients with prostate cancer. The control group consisted of 125 samples from Chinese men. Genotyping was conducted using Taq Man allelic discrimination assays. Statistical analysis was performed using SPSS software. Results No association of NANOG and IL-18 gene polymorphisms and overall prostate cancer susceptibility was detected. The IL-18-607 CC genotype was significantly associated with a higher tumor grade(P = 0.025) and stage(P =0.001). The IL-18-137 GG genotype correlated with a higher tumor grade(P = 0.028) and stage(P = 0.008). The IL-18-137 G allele was significantly more frequent in patients with lymph node metastasis(P = 0.035). The IL-18-607 CC genotype was associated with distant metastasis(P = 0.025). However, no significant association was observed between NANOG polymorphisms and any clinicopathological feature. The Cox proportional hazard model showed that tumor grade and stage grouping were independent prognostic factors in IL-18, while IL-18 polymorphism was not. Polymorphism variants in the IL-18(IL-18-607 and IL-18-137) and NANOG(genotypes AC) genes might be associated with a worse prognosis of patients with prostate cancer.Conclusion NANOG may be associated with the early stages of prostate cancer carcinogenesis. IL-18 and NANOG gene polymorphisms may play a major role in the growth, invasion, and metastasis of prostate cancer. | Shaojun Nong Yangbo Guan Zhiwei Wang Zhongqing Wei Yueping Zhang Jian Ni Chongsheng He Limin Ma Shujun Zhou Wenguang Li | 2018 | Oncology and Translational Medicine2018，4，6： | 2 |
| 6 | Evidence for the paternal mitochondrial DNA in the crucian carp-like fish lineage with hybrid origin显示文摘In terms of taxonomic status,common carp(Cyprinus carpio,Cyprininae)and crucian carp(Carassius auratus,Cyprininae)are different species;however,in this study,a newborn homodiploid crucian carp-like fish(2n=100)(2nNCRC)lineage(F1–F3)was established from the interspecific hybridization of female common carp(2n=100)×male blunt snout bream(Megalobrama amblycephala,Cultrinae,2n=48).The phenotypes and genotypes of 2 n NCRC differed from those of its parents but were closely related to those of the existing diploid crucian carp.We further sequenced the whole mitochondrial(mt)genomes of the 2n NCRC lineage from F1 to F3.The paternal mt DNA fragments were stably embedded in the mt-genomes of F1–F3 generations of 2n NCRC to form chimeric DNA fragments.Along with this chimeric process,numerous base sites of F1–F3 generations of 2 n NCRC underwent mutations.Most of these mutation sites were consistent with the existing diploid crucian carp.Moreover,the mt DNA organization and nucleotide composition of 2n NCRC were more similar to those of the existing diploid crucian carp than those of the parents.The inheritable chimeric DNA fragments and mutant loci in the mt-genomes of different generations of 2nNCRC provided important evidence of the mt DNA change process in the newborn lineage derived from hybridization of different species.Our findings demonstrated for the first time that the paternal mt DNA were transmitted into the mt-genomes of homodiploid lineage,which provided new insights into the existence of paternal mt DNA in the mt DNA inheritance. | Shi Wang Ni Jiao Lu Zhao Meiwen Zhang Pei Zhou Xuexue Huang Fangzhou Hu Conghui Yang Yuqin Shu Wuhui Li Chun Zhang Min Tao Bo Chen Ming Ma Shaojun Liu | 2020 | Science China(Life Sciences)2020，63，1： | 2 |
| 7 | Efficacy of Cigu Xiaozhi pill(慈菇消脂丸) on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathway显示文摘OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill(慈菇消脂丸, CGXZ) on non-alcoholic steatohepatitis(NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase(JNK)/stress-activated protein kinase signalling pathway.METHODS: Sixty male Sprague-Dawley rats were randomly divided into the following groups(10 rats each): blank control, model, low-dose CGXZ,medium-dose CGXZ, high-dose CGXZ, and positive control(treated with SP600125, a JNK inhibitor).The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase d UTP nick-end labelling assay. In addition, the m RNA and protein expression levels of p-JNK,p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR,immunohistochemical and Western blot assays.RESULTS: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group(P < 0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower m RNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group(P < 0.05 or P <0.01).CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH. | MA Yanhua YU Chengzu WU Yan HAN Tao YANG Shaojun SHI Xia | 2021 | Journal of Traditional Chinese Medicine2021，41，1： | 2 |
| 8 | Significant association between IL-18 and OCT4 gene polymorphisms in susceptibility and clinical characteristics of prostate cancer显示文摘Objective Recent studies have shown abnormal expression of octamer-binding transcription factor 4(OCT4) and interleukin-18(IL-18) to be related to cancer. However, the molecular mechanisms by which the IL-18 and OCT4 gene polymorphisms are associated with prostate cancer remain unclear. In this study, we aimed to determine whether the presence of IL-18 and OCT4 polymorphisms were associated with size, grade, tumor, nodes and metastasis(TNM) stage, or survival in patients with prostate cancer.Methods Polymorphisms in OCT4 and IL-18 genes were evaluated to determine susceptibility to prostate cancer in 120 patients. A control group consisted of 125 Chinese participants. Genotyping was performed using TaqMan allelic discrimination assays, and statistical analysis was performed using SPSS. Results No association was found between OCT4 and IL-18 gene polymorphisms and prostate cancer susceptibility. For OCT4 AA and IL-18-607 CC genotypes, there was a significant association with higher tumor grade(P = 0.03 and P = 0.025) and stage(P = 0.04 and P = 0.001). The OCT4 and IL-18-137 GG genotype was correlated with higher tumor grade(P = 0.028) and stage(P = 0.008). Furthermore, OCT4 AA was significantly more frequent in patients with lymph node metastasis(P = 0.02) and distant metastasis(P = 0.01). The Cox proportional hazard model showed that tumor grade and stage grouping were independent prognostic factors but IL-18 and OCT4 polymorphisms were not. Conclusion The OCT4 gene may have a profound effect on prostate cancer risk. Polymorphism variants in the IL-18(IL-18-607 and IL-18-137) and OCT4 genes may be associated with poor prognoses for individuals with prostate cancer. | Shaojun Nong Yangbo Guan Zhiwei Wang Zhongqing Wei Yueping Zhang Jian Ni Chongsheng He Limin Ma Shujun Zhou Wenguang Li | 2019 | Oncology and Translational Medicine2019，5，3： | 2 |
| 9 | Injectable Self‑Healing Adhesive pH‑Responsive Hydrogels Accelerate Gastric Hemostasis and Wound Healing显示文摘Endoscopic mucosal resection(EMR)and endoscopic submucosal dissection(ESD)are well-established therapeutics for gastrointestinal neoplasias,but complications after EMR/ESD,including bleeding and perforation,result in additional treatment morbidity and even threaten the lives of patients.Thus,designing biomaterials to treat gastric bleeding and wound healing after endoscopic treatment is highly desired and remains a challenge.Herein,a series of injectable pH-responsive selfhealing adhesive hydrogels based on acryloyl-6-aminocaproic acid(AA)and AA-g-N-hydroxysuccinimide(AA-NHS)were developed,and their great potential as endoscopic sprayable bioadhesive materials to efficiently stop hemorrhage and promote the wound healing process was further demonstrated in a swine gastric hemorrhage/wound model.The hydrogels showed a suitable gelation time,an autonomous and efficient self-healing capacity,hemostatic properties,and good biocompatibility.With the introduction of AA-NHS as a micro-cross-linker,the hydrogels exhibited enhanced adhesive strength.A swine gastric hemorrhage in vivo model demonstrated that the hydrogels showed good hemostatic performance by stopping acute arterial bleeding and preventing delayed bleeding.A gastric wound model indicated that the hydrogels showed excellent treatment effects with significantly enhanced wound healing with type I collagen deposition,α-SMA expression,and blood vessel formation.These injectable self-healing adhesive hydrogels exhibited great potential to treat gastric wounds after endoscopic treatment. | Jiahui He Zixi Zhang Yutong Yang Fenggang Ren Jipeng Li Shaojun Zhu Feng Ma Rongqian Wu Yi Lv Gang He Baolin Guo Dake Chu | 2021 | Nano-Micro Letters2021，13，5： | 1 |
| 10 | Epidemiology of HIV and sexually transmitted infections in China 显示文摘 | Zhang KongAi Ma ShaoJun Xia DongYan | 2004 | Sexual Health2004，16，4： | 1 |
| 11 | A study on the net greenhouse gas emissions under intensive high-yielding cropland in North China: A case study of winter wheat-summer maize rotation system in Huantai County显示文摘 | Bai Shaojun Liang Long Ma Xun | 2011 | Energy Procedia2011，5，： | 1 |
| 12 | Epidemiology of HIV and sexually transmitted infections in China显示文摘 | Zhang Konglai Ma Shaojun Xia Dongyan | 2004 | Sexual Health2004，16，： | 1 |
| 13 | Effects of gene polymorphisms in the endoplasmic reticulum stress pathway on clinical outcomes of chemoradiotherapy in Chinese patients with nasopharyngeal carcinoma显示文摘有可观的内部个人的 variabil ? 在在鼻咽的癌(NPC ) 的 chemoradiotherapy 回答的 ity 病人 receiv ? ing 一样或类似的治疗协议。在这研究,我们在 endoplasmic 蜂窝胃评估了在基因多型性之间的协会(嗯) 在中国 NPC 病人的压力小径和 chemoradiation 回答。有组织病理学说地遵守的 NPC 的 150 个病人全部、与并发的 chemoradiotherapy 对待的 A 被注册。遗传型在嗯压力小径基因,包括 VCP ( 包含valosin 蛋白质) rs2074549 , HSP90B1 rs17034943 , CANX ( calnexin ) rs7566 , HSPA5 [热吃惊蛋白质家庭 A ( Hsp70 )成员 5 ] rs430397 , CALCR ( calcitonin 受体) rs2528521 ,并且 XBP1 (X盒子绑定蛋白质 1 ) rs2269577 被 Sequenom MassARRAY 分析系统。在放射疗法以后的主要肿瘤和淋巴节点的短期的效果在稳固的肿瘤(石柜) 基于反应评估标准被估计。并且尖锐导致放射的有毒的反应为癌症(RTOG/EORTC ) 的研究和治疗根据放射治疗肿瘤学组或欧洲组织的意见被评估。chemoradiotherapy 的临床的结果上的基因多型性的效果被逻辑回归分析的 chi 平方测试, univariate 和 multivariate 估计。我们发现 CANX rs7566 的 CT 和 CT+CC 遗传型显著地在中国 NPC 病人在 chemoradiotherapy 以后并且与导致放射的 myelosuppression 的出现在 3 个月与主要肿瘤治疗功效被相关。CALCR rs2528521 的 CT 和 CT+CC 遗传型显著地在 chemoradiotherapy 以后在 3 个月与颈的淋巴节点功效被相关。并且 VCP rs2074549 的 CC 和 CT+CC 遗传型显著地与 myelosuppression 的出现被联系。在结论, VCP rs2074549 的 SNP, CANX rs7566 和 CALCR rs2528521 在嗯压力小径基因可以在中国 NPC 病人为 chemoradiotherapy 的临床的结果用作预言者。 | Xiao-bin GUO Wan-le MA Li-juan LIU Yu-ling HUANG Jing WANG Li-hua HUANG Xiang-dong PENG Ji-ye YIN Jin-gao LI Shaojun CHEN Guo-ping YANG Hui WANG Cheng-xian GUO | 2017 | Acta Pharmacologica Sinica2017，38，4： | 1 |
| 14 | Evaluation of antioxidant activities of principal carotenoids available in water spinach (lpomoea aquatica)显示文摘 | FU Hongfei XIE Bijun MA Shaojun | 2011 | Journal of Food Composition and Analysis2011，24，2： | 1 |
| 15 | GL9 from Oryza glumaepatula controls grain size and chalkiness in rice显示文摘Grain size is a key factor influencing grain yield and appearance quality in rice.We identified twelve quantitative trait loci(QTL)for grain length(GL),nine for grain width(GW),and nine for 1000-kernel weight(TKW)using GLU-SSSLs,which are single-segment substitution lines with Oryza glumaepatula as donor parent and Huajingxian 74(HJX74)as recipient parent.Among the QTL,qGL1-2,qGL1-4,qGL9-2,qGW2-2,qGW9-1 and qTKW9-2 contributed to high grain yield.GL9 was identified as a candidate gene for qGL9-2 by map-based cloning and sequencing,and is a novel allele of GS9.The kernel of NIL-gl9was slenderer and longer than that of HJX74,and the TKW and grain yield per plant of NIL-gl9 were higher than those of HJX74.The proportion of grain chalkiness of NIL-gl9 was much lower than that of HJX74.Thus,gl9 increased grain yield and appearance quality simultaneously.Three pyramid lines,NIL-gs3/gl9,NIL-GW7/gl9 and NIL-gw8/gl9,were developed and the kernel of each was longer than that of the corresponding recipient parent lines.The gl9 allele may be beneficial for breeding rice varieties with high grain yield and good appearance quality. | Shaojun Lin Zupei Liu Kui Zhang Weifeng Yang Penglin Zhan Quanya Tan Yajun Gou Shuaipeng Ma Xin Luan Chubing Huang Zhili Xiao Yuanyuan Liu Bihuang Zhu Ruiqing Liang Wenqi Zhou Haitao Zhu Suhong Bu Guifu Liu Guiquan Zhang Shaokui Wang | 2023 | The Crop Journal2023，11，1： | 1 |
| 16 | Myths and Attitudes that Sustain Smoking in China显示文摘 | Shaojun Ma Mai-Anh Hoang Jonathan M. Samet Junfang Wang Cuizhu Mei Xuefang Xu Frances A. Stillman | 2008 | Journal of Health Communication2008，，： | 1 |
| 17 | Epidemiology of HIV and sexually transmitted infections in China 显示文摘 | ZHANG KONGLAI MA SHAOJUN XIA DONGYAN | 2004 | Sexual Health2004，16，4： | 1 |
| 18 | Effect of esterification with fatty acid of β-cryptoxanthin on its thermal stability and antioxidant activity by chemiluminescence method显示文摘 | HongFei Fu BiJun Xie Gang Fan ShaoJun Ma XinRong Zhu SiYi Pan | 2010 | Food Chemistry2010，，3： | 1 |
| 19 | Evaluation of an- tioxidant activities of principal carotenoids available in water spinach ( Ipomoea aquatica) 显示文摘 | Hongfei Fu Bijun Xie Shaojun Ma | 2011 | Journal of Food Composition and Analysis2011，24，： | 1 |
| 20 | RETRACTED ARTICLE: ShRNA-mediated gene silencing of Heat shock protein 70 inhibits human colon cancer cell growth in vitro and in vivo显示文摘 | Haidong Cai Shaojun Yin Fang Ma Fei Yu Dan Li Mingli Lv Yingsheng Chen Zhongwei Lv | 2012 | Molecular and Cellular Biochemistry2012，，1： | 1 |
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