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| 1 | The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility显示文摘Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) cells.Methods: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ kits.Results: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ROS.Conclusions: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy. | Fengjiao Jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang | 2019 | Cancer Biology & Medicine2019，16，1： | 20 |
| 2 | Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds显示文摘最近,我们报导了一条化学途径从老鼠成纤维细胞产生 pluripotent 干细胞。然而,是否化学上导致了 pluripotent 干细胞(CiPSCs ) ,能从要示威的另外的房间类型遗体被导出。用系跟踪,这里,我们首先从成纤维细胞验证 CiPSCs 的产生。下次,我们证明从外胚层的神经干细胞(NSC ) 和从内胚叶的小肠的上皮的房间(IEC ) 能是化学上 reprogrammed 进 pluripotent 干细胞。CiPSCs 源于 NSC 和 IEC 类似于老鼠处于增长率,全球基因表示侧面, epigenetic 地位,自强和区别能力的胚胎的干细胞,和 germline 传播才能。有趣地, pluripotency 基因 Sall4 从不同房间类型,和一样的核心在化学 reprogramming 过程在起始的阶段被表示小分子为 reprogramming 被要求,在从这些多样的房间类型位于化学 reprogramming 下面的分子的机制建议保存。我们的分析也证明这些小分子的使用应该被调整从不同房间类型满足 reprogramming 的要求。一起,这些调查结果证明那条完整的化学 reprogramming 途径能在不同织物起源的房间被使用并且建议化学 reprogramming 是有要延长到更起始的类型的潜力的有希望的策略。 | Junqing Ye Jian Ge Xu Zhang Lin Cheng Zhengyuan Zhang Shan He Yuping Wang Hua Lin Weifeng Yang Junfang Liu Yang Zhao Hongkui Deng | 2016 | Cell Research2016，26，1： | 19 |
| 3 | Variety distribution pattern and climatic potential productivity of spring maize in Northeast China under climate change显示文摘This study was based on the daily meteorological data of 101 meteorological stations from 1971 to 2000 and the 0.25°×0.25° grid data from 1951 to 2100 simulated by RegCM3 under the future A1B climatic scenario published by National Climate Center,in combination with the demand of climatic condition for maize growth in Northeast China.The trajectory of agricultural climatic resources and the effects of climate change on variety distribution and climatic potential productivity of spring maize in Northeast China under future climate change were analyzed.The main agro-climatic resource factors include:the initial date daily average temperature stably passing 10℃(≥10℃),the first frost date,the days of growing period,the ≥10℃ accumulated temperature,and the total radiation and precipitation in the growing period.The results showed that:(1) in the coming 100 years,the first date of ≥10℃ would be significantly advanced,and the first frost date would be delayed.The days of growing period would be extended,the ≥10℃ accumulated temperature and the total radiation would be significantly increased.However,no significant change was found in precipitation.(2) Due to the climate change,the early-maturing varieties will be gradually replaced by late-maturing varieties in Northeast China,and the planting boundaries of several maize varieties would be extended northward and eastward.(3) There would be a significant change in the climatic potential productivity of maize in Northeast China with the high-value gradually moving towards northeast.(4) It was an effective way to increase the climatic potential productivity of maize by appropriate adjustment of sowing date. | YUAN Bin GUO JlanPing YE MingZhu ZHAO JunFang | 2012 | Chinese Science Bulletin2012，57，26： | 16 |
| 4 | I-IBV life cycle is restricted in mouse hepatocytes .~xpressing human NTCP显示文摘 | Hanjie Li Qiuyu Zhuang Yuze Wang Tianyi'ng Zhang Jinghua Zhao Yali Zhang Junfang Zhang Yi Lin Quan Yuan Ningshao Xia Jiahuai Han | 2014 | Cellular & Molecular Immunology2014，11，2： | 14 |
| 5 | Fields experiments in North China show no decrease in winter wheat yields with night temperature increased by 2.0-2.5°C显示文摘The trends of daily maximum and minimum temperature in global warming indicated that the daily minimum temperature (Tmin) has risen more than twice as fast as the daily maximum temperature (Tmax) during the 20th century. Most researchers have focused on how the crops respond to daily mean temperature, whereas few controlled experiments were carried out to investigate how the crops respond to the Tmin rise. In particular, no experiment research has reported on how crops respond to the higher night temperature, which was the main trend in the climate warming. Taking winter wheat as the test crop, we investigated how the winter wheat growth and yields responded to the higher night temperature. In the field experiments, infrared heaters were used to increase higher night temperature (HNT) by about 2.5°C in contrast to the normal night temperature (CK) in two whole growth durations of winter wheat in 2008-2009 and 2009-2010 in North China. The results indicated that, compared to the CK treatment, winter wheat yield did not decline in HNT treatment, which increased temperatures by 2.0 2.5°C in both Warmer year (WY) and Colder year (CY). Furthermore, winter wheat yield in CY increased significantly in HNT treatment. HNT treatment in CY could significantly promote tillering and increase the effective panicles, which increased grain yield significantly (by more than 30% compared with CK). HNT treatment in CY contributed to an increase in the effective panicles and Kernels significantly, although making a significant reduction in 1000-grain weight, but did not lead to the yield decline. Under the HNT treatment, the whole growth duration of the winter wheat was shortened and the phenological dates were earlier except for the beginning of overwintering; the beginnings of the overwintering phase were postponed substantially and the ends of the overwintering phase were ahead of date compared to CK, which shortened the duration of overwintering considerably. We draw on our own studies to show examples of higher night temperature impact on winter wheat in a relatively cold year and relatively warm year in North China. Our results refer to winter wheat in North China, not all main winter wheat producing regions, in Huang-Huai and Southwest of China. Some uncertainties of our predictions derive from fast progress in crop breeding, the variability of climate, and the role of adaptive actions in the future. As expected, the adaptation measures should be considered to cope with the impacts of global warming on crops, and further research and assessments should be conducted. | FANG ShiBo TAN KaiYan REN SanXue ZHANG XinShi ZHAO JunFang | 2012 | Science China Earth Sciences2012，55，6： | 13 |
| 6 | Feasibility and value of quantitative dynamic contrast enhancement MR imaging in the evaluation of sinonasal tumors显示文摘 | Xian Junfang Du Huarui Wang Xinyan Yan Fei Zhang Zhengyu Hao Hui Zhao Bo Tong Yajian Zhang Jue Han Demin | 2014 | Chinese Medical Journal2014，，12： | 13 |
| 7 | Value of magnetic resonance imaging including dynamic contrast-enhanced magnetic resonance imaging in differentiation between inverted papilloma and malignant tumors in the nasal cavity显示文摘 | Wang Xinyan Zhang Zhengyu Chen Xiaoli Li Jing Xian Junfang | 2014 | Chinese Medical Journal2014，，9： | 13 |
| 8 | Symmetry and tendency judgment of Ms≥8. 0 strong earthquakes in Chile显示文摘The Ms≥8. 0 strong earthquakes occurring in Chile since 1800 were analyzed using the ternary,quaternary,and quinary commensurability methods and the butterfly structure diagram,and it was believed that the earthquake signal in Chile in 2014 is relatively strong,a large earthquake is likely to occur in Chile in2014. An analysis of spatial epicenter migrations showed that the longitudinal and latitudinal epicenter migrations have symmetry and synchronism,and there were five obvious northward migrations and four southward migrations. The symmetry axis of the longitudinal migrations is at about 71. 7°W and that of the latitudinal migrations is at about 30°S; these spatial symmetry axes are located at the subduction zone on the western margin of South America,where two major plates( the Nazca Plate and the South American Plate) converge. | Jin Junfang Yin Shuyan Yan Junping | 2014 | Geodesy and Geodynamics2014，5，1： | 12 |
| 9 | Analysis and improvement of missile three-loop autopilots显示文摘The non-minimum phase feature of tail-controlled missile airframes is analyzed.Three selection strategies for desired performance indexes are presented.An acceleration autopilot design methodology based on output feedback and optimization is proposed.Performance and robustness comparisons between the two-loop and classical three-loop topologies are made.Attempts to improve the classical three-loop topology are discussed.Despite the same open-loop structure,the classical three-loop autopilot shows distinct characteristics from a two-loop autopilot with PI compensator.Both the two-loop and three-loop topologies can stabilize a static unstable missile.However, the finite actuator resource is the crucial factor dominating autopilot function. | Lin Defu Fan Junfang Qi Zaikang Mou Yu | 2009 | Journal of Systems Engineering and Electronics2009，20，4： | 10 |
| 10 | Two-photon-excited near-infrared emissive carbon dotsas multifunctional agents for fluorescence imaging andphotothermal therapy显示文摘C dots (CDs) have shown great potential in bioimaging and phototherapy.However, it is challenging to manipulate their fluorescent properties andtherapeutic efficacy to satisfy the requirements for clinic applications. In thisstudy, we prepared S, Se-codoped CDs via a hydrothermal method anddemonstrated that the doping resulted in excitation wavelength-independentnear-infrared (NIR) emissions of the CDs, with peaks at 731 and 820 nm.Significantly, the CDs exhibited a photothermal conversion efficiency of -58.2%,which is the highest reported value for C nanostructures and is comparable tothat of Au nanostructures. Moreover, the CDs had a large two-photon absorptioncross section (-30,045 GM), which allowed NIR emissions and the photothermalconversion of the CDs through the two-photon excitation (TPE) mechanism. Invitro and in vivo tests suggested that CDs can function as new multi functionalphototheranostic agents for the TPE fluorescence imaging and photothermaltheraov of cancer cells. | Minhuan Lan Shaojing Zhao Zhenyu Zhang Li Yan Liang Guo Guangle Niu Jinfeng Zhang Junfang Zhao Hongyan Zhang Pengfei Wang Guangyu Zhu Chun-Sing Lee Wenjun Zhang | 2017 | Nano Research2017，10，9： | 9 |
| 11 | Modulation of Gut Microbiota in Pathological States显示文摘人类微生态是寄居在人体中的微生物聚集体,且主要存在于胃肠道(GIT)中。肠道微生态随着人体发育而演化,并在人类健康和疾病中起着重要作用。近年来,由于微生态会影响宿主代谢、生理学和免疫系统发育,而且微生态紊乱可能导致许多疾病,其越来越受到人们的关注。肠道微生态可能与恶性肿瘤有一定联系,如胃癌和结直肠癌;也可能与其他一些疾病有关,如非酒精性脂肪肝(NAFLD)、被称为工业化世界'生活方式疾病'的肥胖和糖尿病、冠心病以及中枢神经系统紊乱。虽然分子技术革命为我们更准确地研究肠道微生态提供了必要的工具,但是我们需要更精确地阐明其与某些人类疾病病理变化的关系,明确微生态在不同疾病中的作用是新的治疗策略发展的基础。本文概述了肠道微生态对人类健康的重要影响以及调整肠道菌群结构的潜在用途,如菌群移植用于治疗耐药艰难梭菌(C.difficile)的感染。通过微生态干预调整肠道区域以改善人类健康的概念虽刚刚兴起,但其治疗意义显著。因此,抑制有害菌、促进有益菌可能会保护人类健康,并且这些努力将为探索发展更加合理的治疗方案打下基础。 | Yulan Wang BaohongWang Junfang wu xiangyang Jiang Huiru Tang Ole H. Nielsen | 2017 | Engineering2017，3，1： | 9 |
| 12 | Design and experimental study of the control system for precision seed-metering device显示文摘The precision seeding technique has been developed at full speed along with the continuous development of new agricultural technologies,especially those concerning cultivated patterns.The seed-metering device is the key component of a precision seeder.A ground wheel is used to drive the seed-metering device of the conventional direct seeder.However,the wheel bears high resistance and easily slips.Moreover,the adjustment of the precision seeder’s seeding rate is more difficult.In order to solve these problems,a control system which could keep the rotational speed of the seed-metering device consistent with the seeder’s working speed for the precision seed-metering device was designed.The control system includes a Hall sensor,a single chip microcomputer system,a motor control module,a stepper motor and a display.The control system used a Hall sensor to measure the seeder’s working speed and employed a single chip microcomputer system to predict the rotational speed of seed-metering device.It would then determine the relationship between the seeder’s working speed and the rotational speed of the seed metering-device according to the seeder’s working state,distance between seeds and the requirement of sowing rate.The system could effectively reduce the influence of inhomogeneous sowing caused by the ground wheel’s slipping.The system was found to be reliable by the experiment.The seeding control system could also make the speed of the seed-metering device and seeder’s uniform,improving the uniformity of the amount of seeding,and achieving the goal of design.This new design provides a platform to solve problems of the seed-metering device and the seeder. | Zhai Jianbo Xia Junfang Zhou Yong Zhang Shun | 2014 | International Journal of Agricultural and Biological Engineering2014，7，3： | 9 |
| 13 | Heavy metal(loid) pollution in mine wastes of a Carlin-type gold mine in southwestern Guizhou, China and its environmental impacts显示文摘Mercury pollution resulting from artisanal gold mining is a serious environmental problem in many developing countries. In this study, we analyzed heavy metal(loid) contamination in mine wastes from a Carlin-type gold mine in southwestern Guizhou, China. Highly elevated levels of As, Hg, Tl, Sb, and Cd—up to 5844, 28,29, 581 and 3.0 mg/kg, respectively—were observed in the mine wastes, but no significant accumulation of Cr, Ni, Cu,Zn, and Pb was found. The smelting process of gold ores had significant impacts on the enrichment of As, Tl, and Hg in the mine wastes. Due to the significant metal(loid)pollution in this gold mine, the ecological risks are classified as ‘‘very high.'' Hg and As are the major pollutants with a relative contribution of 55.9 % and 24.2 % to the risk index, respectively. Phytoremediation could be used to reduce heavy metal contamination and recycle the gold simultaneously. Hg–As–Tl pollution should be considered in gold extraction at Carlin-type gold mines. | Ping Li Junfang Zhang Jianxu Wang Zhonggen Li | 2015 | Chinese Journal Of Geochemistry2015，34，3： | 9 |
| 14 | Actin cytoskeleton-dependent pathways for ADMA-induced NF-κB activation and TGF-β high expression in human renal glomerular endothelial cells显示文摘不对称的 dimethylarginine (ADMA ) ,一个内长的氮的氧化物 synthase 禁止者,被认为是在长期的肾疾病(CKD ) 的前进的一个独立风险因素。它能由增加转变生长因素(TGF )-1 表示导致肾纤维变性,但是它的分子的机制是不清楚的。现在的学习的目的是在人的肾的 glomerular endothelial 房间(HRGEC ) 在导致 ADMA 的 TGF-1 高表示调查肌动朊细胞骨架的角色。压力纤维的结构被 immunofluorescence 设想,原子 factor-B (NF-B )DNA 有约束力的活动被 electrophoretic 活动性移动试金估计, TGF-1 表示被西方的污点分析估计。结果证明 ADMA 导致了压力纤维, NF-B 的 DNA 绑定,和 TGF-1 的增加的表示的汇编。当肌动朊细胞骨架的动力学被使解聚肌动朊的代理人 cytochalasin 使不安时, D 和稳定肌动朊的代理人 jasplakinolide,或压力纤维的脱离由 nicotineamide 腺嘌 dinucleotide 磷酸盐 oxidase 禁止者 apocynin 和 p38 绑激活 mitogen 的蛋白质 kinase 禁止者 SB203580, NF-B 的导致 ADMA 的 DNA 绑定和 TGF-1 表示被禁止。这些结果在 HRGEC 在导致 ADMA 的 NF-B 激活和 TGF-1 高表示揭示了肌动朊细胞骨架依赖者机制。肌动朊细胞骨架的特定的指向可以是有用策略在 CKD 阻止激活 ADMA 的肾纤维变性。 | Liyan Wang Dongliang Zhang Junfang Zheng Yiduo Feng Yu Zhang Wenhu Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，11： | 9 |
| 15 | Synthesis,luminescent properties,and theoretical study of novel Sm^(3+) and Dy^(3+) complexes containing β-diketone and 1,10-phenanthroline显示文摘Two new ternary complexes of 1-(2-thienyl)-3-(p-phenylethynylphenyl)-1,3-propanedione(HTPP) and 1,10-phenanthroline(phen) with Sm3+ and Dy3+ were synthesized.The composition of the ternary complexes was characterized as Sm(TPP)3phen and Dy(TPP)3phen,respectively,by infrared(IR) spectra,chemical analysis,elemental analysis,and thermodynamic analysis.At room temperature,under UV light excitation,the Sm3+ and Dy3+ complexes exhibit characteristic emissions of the central ions.It is found that the fluorescence intensity of Sm(TPP)3phen is stronger than that of Dy(TPP)3phen.In order to explain this phenomenon,an accurate quantum chemistry calculation was carried out,and the result is in good agreement with the experiment data. | LIU Xingwang GAO Saishengtai WANG Li SHEN Leijun JIANG Jiadong GAO Junfang | 2011 | Rare Metals2011，30，1： | 7 |
| 16 | Simulating Net Carbon Budget of Forest Ecosystems and Its Response to Climate Change in Northeastern China Using Improved FORCCHN显示文摘As dominant biomes,forests play an important and indispensable role in adjusting the global carbon balance under climate change.Therefore,there are scientific and political implications in investigating the carbon budget of forest ecosystems and its response to climate change.Here we synthesized the most recent research progresses on the carbon cycle in terrestrial ecosystems,and applied an individual-based forest ecosystem carbon budget model for China(FORCCHN) to simulate the dynamics of the carbon fluxes of forest ecosystems in the northeastern China.The FORCCHN model was further improved and applied through adding variables and modules of precipitation(rainfall and snowfall) interception by tree crown,understory plants and litter.The results showed that the optimized FORCCHN model had a good performance in simulating the carbon budget of forest ecosystems in the northeastern China.From 1981 to 2002,the forests played a positive role in absorbing carbon dioxide.However,the capability of forest carbon sequestration had been gradually declining during the the same period.As for the average spatial distri-bution of net carbon budget,a majority of the regions were carbon sinks.Several scattered areas in the Heilongjiang Province and the Liaoning Province were identified as carbon sources.The net carbon budget was apparently more sensitive to an increase of air temperature than change of precipitation. | ZHAO Junfang YAN Xiaodong JIA Gensuo | 2012 | Chinese Geographical Science2012，22，1： | 6 |
| 17 | Research and Practice of Dynamic Network Security Architecture for IaaS Platforms显示文摘Network security requirements based on virtual network technologies in IaaS platforms and corresponding solutions were reviewed.A dynamic network security architecture was proposed,which was built on the technologies of software defined networking,Virtual Machine(VM)traffic redirection,network policy unified management,software defined isolation networks,vulnerability scanning,and software updates.The proposed architecture was able to obtain the capacity for detection and access control for VM traffic by redirecting it to configurable security appliances,and ensured the effectiveness of network policies in the total life cycle of the VM by configuring the policies to the right place at the appropriate time,according to the impacts of VM state transitions.The virtual isolation domains for tenants’VMs could be built flexibly based on VLAN policies or Netfilter/Iptables firewall appliances,and vulnerability scanning as a service and software update as a service were both provided as security supports.Through cooperation with IDS appliances and automatic alarm mechanisms,the proposed architecture could dynamically mitigate a wide range of network-based attacks.The experimental results demonstrate the effectiveness of the proposed architecture. | Lin Chen Xingshu Chen Junfang Jiang Xueyuan Yin Guolin Shao | 2014 | Tsinghua Science and Technology2014，19，5： | 6 |
| 18 | Audiological evaluation in Chinese patients with mitochondrial encephalomyopathies显示文摘 | Liu Yuhe Xue Junfang Zhao Danhua Chen Li Yuan Yun Wang Zhaoxia | 2014 | Chinese Medical Journal2014，，12： | 6 |
| 19 | SOA-based RFID public services infrastructure:architecture and its core services显示文摘Radio frequency identification(RFID)has prominent advantages compared with other autoidentification technologies.Combining RFID with network technology,physical object tracking and information sharing can possibly be carried out in an innovative way.Regarding open-loop RFID applications,RFID public services infrastructure(PSI)is presented,PSI architecture is designed,and service modules are implemented,and a demonstrative application system,blood management and traceability system,is studied to verify PSI.Experimental results show the feasibility of the proposed architecture and the usability of PSI framework software. | Zeng Junfang Li Ran Luo Jin Liu Yu | 2009 | Journal of Systems Engineering and Electronics2009，20，3： | 5 |
| 20 | Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis显示文摘An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a 'reporter' gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species. | Junfang Lin Mingyao Zheng Jie Wang Wei Shu Liqiong Guo | 2008 | Progress in Natural Science:Materials International2008，18，7： | 5 |
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