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| 1 | Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization显示文摘Three-dimensional(3D)printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths,in order to induce improved bone regeneration in defects with a critical size.Given that the successful bone regeneration requires both excellent osteogenesis and vascularization,endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization.In this investigation,customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing ofβ-tricalcium phosphate and osteogenic peptide(OP)containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks.The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone.Angiogenic peptide(AP)containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability.A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds.Both rat endothelial cells(ECs)and rat bone marrow derived mesenchymal stem cells(MSCs)showed high viability on scaffolds.Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP.The in vivo results showed that,toward scaffolds containing both AP and OP,the quick release of AP induced obvious angiogenesis in vivo,while the sustained OP release significantly improved the new bone formation.This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions. | Chong Wang Jiahui Lai Kai Li Shaokui Zhu Bingheng Lu Jia Liu Yujin Tang Yen Wei | 2021 | Bioactive Materials2021，6，1： | 7 |
| 2 | Heterosis and combining ability evaluation for growth traits of blunt snout bream(Megalobrama amblycephala)when crossbreeding three strains显示文摘The blunt snout bream(Megalobrama amblycephala)is a major aquaculture species in the Chinese freshwater polyculture system.In this study,complete diallel crossing of three strains(i.e.,Liangzi[LZ],Poyang[PY]and Yuni[YN])was used to evaluate the combining ability and heterosis effect of intraspecific crossbreeding on the growth performance(i.e.,body weight,total length,body length,body height,and Fulton’s coefficient of condition).The offspring produced from the three strains were reared in a communal pond for 20 months,and nine microsatellites were used to assess their pedigree.Of the749 offspring,708(94.53%)could be assigned directly to a single parental pair.Significant differences were observed in body weight(BW)among the different combinations,indicating that the combining ability of BW from each strain was significantly different.The general combining ability(GCA)of BW from the sire was much higher than that from the dam.The GCA for the dam and sire of the LZ and YN were the largest and the smallest,respectively.The special combining ability of YN$9 PY#was the largest,and YN$9 PY#showed significantly positive heterosis effects on all F1growth traits,except for Fulton’s condition coefficient(K)(P\0.05).In addition,significant positive linear correlations were found between the mean BW of corresponding progeny and the H E of the parental generation(y=600.7x-29.472,r=0.8651,P=0.0026),H O(y=1206.9x-518.14,r=0.7436,P=0.0216),and the polymorphism information content(y=1021.3x-331.31,r=0.8245,P=0.0063)as determined by a correlative test.The findings obtained from the study will be important for future considerations of M.amblycephala stock improvement programs. | Wei Luo Cong Zeng Shaokui Yi Nicholas Robinson Weimin Wang Zexia Gao | 2014 | Chinese Science Bulletin2014，59，9： | 7 |
| 3 | Natural variations in grain length 10(GL10)regulate rice grain size显示文摘Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characterized.Here,we describe the grain length QTL GL10,encoding MADS56,which positively regulates grain length and grain weight.A natural allelic variation of NIL-gl10,containing an~1.0-kb deletion in the first exon that abolishes its transcription,results in shorter grain length,lower grain weight and delayed flowering in gl10 plants.The knockout of GL10 in the HJX74 background leads to grain phenotypes similar to that of NIL-gl10,while overexpression of GL10 results in increased grain length and weight and earlier heading date.GL10 regulates grain length by promoting greater longitudinal cell growth in the grain glume.Additionally,GL10 participates in the regulation of gibberellic acid(GA)signaling pathway genes in young panicle tissues.Analysis of GL10 haplotypes shows obvious divergence between the japonica and indica lineages.Our findings reveal an allelic variation of GL10 that may explain differences in grain length among modern cultivars and could be used to breed rice varieties with optimized grain shape. | Penglin Zhan Shuaipeng Ma Zhili Xiao Fangping Li Xin Wei Shaojun Lin Xiaoling Wang Zhe Ji Yu Fu Jiahao Pan Mi Zhou Yue Liu Zengyuan Chang Lu Li Suhong Bu Zupei Liu Haitao Zhu Guifu Liu Guiquan Zhang Shaokui Wang | 2022 | Journal of Genetics and Genomics2022，49，5： | 3 |
| 4 | Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice显示文摘Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis. | Mumei Wang Xiaopei Zhu Guoqing Peng Minglong Liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan Liu Xianrong Xie Shaokui Wang Aimin Wu Chuxiong Zhuang Hai Zhou | 2022 | Molecular Plant2022，15，6： | 2 |
| 5 | Very high upper critical fields of F-doped Fe-based layered superconductors NdO_(0.88)F_(0.12)FeAs and CeO_(0.88)F_(0.12)FeAs显示文摘Using the solid state reaction method, we have synthesized the polycrystalline F-doped NdO0.88F0.12FeAs and CeO0.88F0.12FeAs with the superconducting transition temperatures at about 48 and 40 K, respectively. To obtain the upper critical field Hc2 of Nd(Ce)O0.88F0.12FeAs samples, we measured the electrical resistivity under magnetic field up to 14 T. Based on the Werthamer-Helfand-Hohenberg (WHH) rela-tion together with the Hc2(T) curves in a relatively high field, we estimated that these superconductors have a rather high upper critical field of about 115 T for Nd-based and 107 T for Ce-based samples, indicating the similarities between these ReO1-xFxFeAs (Re = rare earth element) superconductors and high Tc cuprate su-perconductors. | WU Dan, LI Zheng, SU ShaoKui, DONG Jing, LI Gang, HU WanZheng, ZHENG Ping, CHEN GenFu, LUO JianLin & WANG NanLin Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，6： | 2 |
| 6 | Enhanced uptake and anti-maturation effect of celastrol-loaded mannosylated liposomes on dendritic cells for psoriasis treatment显示文摘Psoriasis is an autoimmune skin disease in which dendritic cells(DCs) trigger the progression of psoriasis by complex interactions with keratinocytes and other immune cells. In the present study,we aimed to load celastrol, an anti-inflammatory ingredient isolated from Chinese herbs, on mannosylated liposomes to enhance DC uptake as well as to induce DC tolerance in an imiquimod-induced psoriasis-like mouse model. Mannose was grafted onto liposomes to target mannose receptors on DCs. The results demonstrated that compared with unmodified liposomes, DCs preferred to take up more fluorescence-labeled mannosylated liposomes. After loading celastrol into mannose-modified liposomes,they effectively inhibited the expression of maturation markers, including CD80, CD86 and MHC-II, on DCs both in vitro and in vivo. Additionally, after intradermal injection with a microneedle, celastrolloaded mannose-modified liposomes(CEL-MAN-LPs) achieved a superior therapeutic effect compared with free drug and celastrol-loaded unmodified liposomes in the psoriasis mouse model in terms of the psoriasis area and severity index, histology evaluation, spleen weight, and expression of inflammatory cytokines. In conclusion, our results clearly revealed that CEL-MAN-LPs was an effective formulation for psoriasis treatment and suggested that this treatment has the potential to be applied to other inflammatory diseases triggered by activated DCs. | Long Xi Zibei Lin Fen Qiu Shaokui Chen Ping Li Xin Chen Zhenping Wang Ying Zheng | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 2 |
| 7 | Fine Mapping of QTLs for Stigma Exsertion Rate from Oryza glaberrima by Chromosome Segment Substitution显示文摘Stigma exsertion is an important trait for outcrossing ability in rice.Stigma exsertion rate(SER)of male sterile lines(MSLs)is a key factor affecting F1-seed production in hybrid rice.In this study,seven QTLs for SER were detected on five chromosomes using a set of single-segment substitution lines(SSSLs)derived from O.glaberrima.Three of the QTLs were mapped in the estimated intervals of 92.5–333.0 kb.qSER-5 was located in a substitution segment of 92.5 kb.qS ER-1 b and qS ER-8 b were respectively limited to 333.0 kb and 107.5 kb by secondary substitution mapping.qSER-1 b and qSER-3 had bigger additive effects of 11.5%and 11.9%,respectively,while the other five QTLs had smaller additive effects from 5.7%to 8.6%.Open reading frames were identified in the regions of qS ER-5 and qSER-8 b in O.sativa and O.glaberrima genomes.Fine mapping of the QTLs laid a foundation for the cloning of genes,and QTLs for SER will be used to develop MSLs with strong ability of outcrossing. | TAN Quanya ZHU Haitao LIU Hui NI Yuerong WU Shengze LUAN Xin LIU Junwei YANG Weifeng YANG Zifeng ZENG Ruizhen LIU Guifu WANG Shaokui ZHANG Guiquan | 2022 | Rice science2022，29，1： | 1 |
| 8 | GL9 from Oryza glumaepatula controls grain size and chalkiness in rice显示文摘Grain size is a key factor influencing grain yield and appearance quality in rice.We identified twelve quantitative trait loci(QTL)for grain length(GL),nine for grain width(GW),and nine for 1000-kernel weight(TKW)using GLU-SSSLs,which are single-segment substitution lines with Oryza glumaepatula as donor parent and Huajingxian 74(HJX74)as recipient parent.Among the QTL,qGL1-2,qGL1-4,qGL9-2,qGW2-2,qGW9-1 and qTKW9-2 contributed to high grain yield.GL9 was identified as a candidate gene for qGL9-2 by map-based cloning and sequencing,and is a novel allele of GS9.The kernel of NIL-gl9was slenderer and longer than that of HJX74,and the TKW and grain yield per plant of NIL-gl9 were higher than those of HJX74.The proportion of grain chalkiness of NIL-gl9 was much lower than that of HJX74.Thus,gl9 increased grain yield and appearance quality simultaneously.Three pyramid lines,NIL-gs3/gl9,NIL-GW7/gl9 and NIL-gw8/gl9,were developed and the kernel of each was longer than that of the corresponding recipient parent lines.The gl9 allele may be beneficial for breeding rice varieties with high grain yield and good appearance quality. | Shaojun Lin Zupei Liu Kui Zhang Weifeng Yang Penglin Zhan Quanya Tan Yajun Gou Shuaipeng Ma Xin Luan Chubing Huang Zhili Xiao Yuanyuan Liu Bihuang Zhu Ruiqing Liang Wenqi Zhou Haitao Zhu Suhong Bu Guifu Liu Guiquan Zhang Shaokui Wang | 2023 | The Crop Journal2023，11，1： | 1 |
| 9 | Toughened Polypropylene with Balanced Rigidity(I):Preparation and Chemical Structure of Toughening Master Batch显示文摘 | Zhang Hongjie Wang Jingwu Cao Shaokui | 2000 | Polymers for Advanced Technologies2000，11，7： | 1 |
| 10 | Reactive, intumescent, halogen‐free flame retardant for polypropylene显示文摘 | Guixun Li Wanjie Wang Shaokui Cao Yanxia Cao Jingwu Wang | 2014 | J Appl Polym Sci2014，，7： | 1 |
| 11 | Melting behaviors, isothermal and non-isothermal crystallization kinetics of nylon 1212显示文摘 | Minying Liu Qingxiang Zhao Yudong Wang Chenggui Zhang Zhishen Mo Shaokui Cao | 2003 | Polymer2003，，8： | 1 |
| 12 | Hierarchical N-Doped Porous Carbons for Zn–Air Batteries and Supercapacitors显示文摘Nitrogen-doped carbon materials with a large specific surface area,high conductivity,and adjustable microstructures have many prospects for energy-related applications.This is especially true for N-doped nanocarbons used in the electrocatalytic oxygen reduction reaction(ORR)and supercapacitors.Here,we report a low-cost,environmentally friendly,large-scale mechanochemical method of preparing N-doped porous carbons(NPCs)with hierarchical micro-mesopores and a large surface area via ball-milling polymerization followed by pyrolysis.The optimized NPC prepared at 1000°C(NPC-1000)offers excellent ORR activity with an onset potential(Eonset)and half-wave potential(E1/2)of 0.9 and 0.82 V,respectively(vs.a reversible hydrogen electrode),which are only approximately 30 mV lower than that of Pt/C.The rechargeable Zn–air battery assembled using NPC-1000 and the NiFe-layered double hydroxide as bifunctional ORR and oxygen evolution reaction electrodes offered superior cycling stability and comparable discharge performance to RuO2 and Pt/C.Moreover,the supercapacitor electrode equipped with NPC prepared at 800℃ exhibited a high specific capacity(431 F g^−1 at 10 mV s^−1),outstanding rate,performance,and excellent cycling stability in an aqueous 6-M KOH solution.This work demonstrates the potential of the mechanochemical preparation method of porous carbons,which are important for energy conversion and storage. | Beibei Guo Ruguang Ma Zichuang Li Shaokui Guo Jun Luo Minghui Yang Qian Liu Tiju Thomas Jiacheng Wang | 2020 | Nano-Micro Letters2020，12，2： | 1 |
| 13 | Toughened polypropylene with hahnced rigidity: (Ⅰ)Preparation and ehanical structure of tlxaghening master batch显示文摘 | Hongjie Zhang Jingwu Wang Shaokui Cao | 2000 | Polym Adv Technol2000，11，： | 1 |
| 14 | Toughened polypropylene ,with balanced rigidity( I ) :Preparation and chemical structure of toughening master batch 显示文摘 | Zhang Hongjie Wang Jingwu Cao Shaokui | 2000 | Polym Adv Technol2000，11，7： | 1 |
| 15 | Ring-opening metathesis polymerization of norbornene derivatives for multifunctionalized all-optical photorefractive polymers with a non-conjugated main chain显示文摘 | Haiyan Pei Wei Li Yingliang Liu Dongfang Wang Jin Wang Jun Shi Shaokui Cao | 2011 | Polymer2011，，1： | 1 |
| 16 | Golgi-localized MORN1 promotes lipid droplet abundance and enhances tolerance to multiple stresses in Arabidopsis显示文摘Lipid droplet(LD)in vegetative tissues has recently been implicated in environmental responses in plants,but its regulation and its function in stress tolerance are not well understood.Here,we identified a Membrane Occupation and Recognition Nexus 1(MORN1)gene as a contributor to natural variations of stress tolerance through genome-wide association study in Arabidopsis thaliana.Characterization of its loss-of-function mutant and natural variants revealed that the MORN1 gene is a positive regulator of plant growth,disease resistance,cold tolerance,and heat tolerance.The MORN1 protein is associated with the Golgi and is also partly associated with LD.Protein truncations that disrupt these associations abolished the biological function of the MORN1 protein.Furthermore,the MORN1 gene is a positive regulator of LD abundance,and its role in LD number regulation and stress tolerance is highly linked.Therefore,this study identifies MORN1 as a positive regulator of LD abundance and a contributor to natural variations of stress tolerance.It implicates a potential involvement of Golgi in LD biogenesis and strongly suggests a contribution of LD to diverse processes of plant growth and stress responses. | Zhan Li Yue Gao Jiapei Yan Shuai Wang Shu Wang Yuanyuan Liu Shaokui Wang Jian Hua | 2023 | Journal of Integrative Plant Biology2023，65，8： | 0 |
| 17 | Uptake and traffcking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model显示文摘Psoriasis is an autoimmune infammatory disease,where dendritic cells(DCs)play an important role in its pathogenesis.In our previous work,we have demonstrated that topical delivery of curcumin-loaded poly(lactic-co-glycolic acid)(PLGA)nanoparticles(NPs)could treat Imiquimod(IMQ)-induced psoriasis-like mice.The objective of this study is to further elucidate biofate of PLGA NPs after intradermal delivery including DCs uptake,and their further traffcking in psoriasis-like mice model by using fuorescence probes.Two-sized DiO/DiI-loaded PLGA NPs of 50±4.9 nm(S-NPs)and 226±7.8 nm(L-NPs)were fabricated,respectively.In vitro cellular uptake results showed that NPs could be internalized into DCs with intact form,and DCs preferred to uptake larger NPs.Consistently,in vivo study showed that L-NPs were more captured by DCs and NPs were frstly transported to skindraining lymph nodes(SDLN),then to spleens after 8 h injection,whereas more S-NPs were transported into SDLN and spleens.Moreover,FRET imaging showed more structurally intact L-NPs distributed in skins and lymph nodes.In conclusion,particle size can affect the uptake and traffcking of NPs by DCs in skin and lymphoid system,which needs to be considered in NPs tailing to treat infammatory skin disease like psoriasis. | Zibei Lin Long Xi Shaokui Chen Jinsong Tao Yan Wang Xin Chen Ping Li Zhenping Wang Ying Zheng | 2021 | Acta Pharmaceutica Sinica B2021，11，4： | 0 |
| 18 | High-Quality de novo Genome Assembly of Huajingxian 74, a Receptor Parent of Single Segment Substitution Lines显示文摘Rice(Oryza sativa L.)is grown nearly worldwide and provides the staple food for more than half of the global population(Luo et al,2017).The genomes of several cultivated rice varieties including Nipponbare(NPB)(Kawahara et al,2013;Sakai et al,2013),IR64(Tanaka et al,2020),93-11(Zhang et al,2018)and R498(Du et al,2017)at chromosome level,and Minghui 63 and Zhenshan 97(Zhang et al,2016)at scaffold level have been assembled,annotated and released,among which the R498 and NPB genomes are widely used as reference genomes in rice research.However,there are thousands of rice cultivars,landraces and wild rice varieties in the world with dramatically different genetic backgrounds,and the genomes of native rice varieties in South China,which is one of the major rice production areas in China,have not been de novo assembled.Huajingxian 74(HJX74)is an indica rice variety bred in South China Agricultural University,Guangdong Province with widely environmental adaptability and high yield(www.ricedata.cn/variety/varis/602548.htm).HJX74 exhibits significant phenotypic and genetic differences from those varieties whose whole genomes have been properly sequenced and assembled(Fig.1). | LI Fangping GAO Yanhao WU Bingqi CAI Qingpei ZHAN Pengling YANG Weifeng SHI Wanxuan LI Xiaohua YANG Zifeng TAN Quanya LUAN Xin ZHANG Guiquan WANG Shaokui | 2021 | Rice science2021，28，2： | 0 |
| 19 | Low-temperature antiferromagnetic order of Ni_4 single-molecule magnets显示文摘The low-temperature specific heat of Ni4 single-molecule magnets (SMMs) is measured in a PPMS He-3 system. The tem- perature-dependent specific heat of Ni4 SMMs shows an antiferromagnetic phase transition at 0.91 K. The experimental results are in good consistency with Monte Carlo simulation of 3D-Ising model, indicating two independent diamond-like antiferro-magnetic structures with an exchange energy J = 0.020 K in Ni4 system. | ZUO JunWei LIU HaiQing LI YanRong SU ShaoKui WANG YunPing | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，1： | 0 |
| 20 | An Efficient Trap Passivator for Perovskite Solar Cells:Poly(propylene glycol)bis(2‑aminopropyl ether)显示文摘Perovskite solar cells(PSCs)are regarded as promising candidates for future renewable energy production.High-density defects in the perovskite films,however,lead to unsatisfactory device performances.Here,poly(propylene glycol)bis(2-aminopropyl ether)(PEA)additive is utilized to passivate the trap states in perovskite.The PEA molecules chemically interact with lead ions in perovskite,considerably passivate surface and bulk defects,which is in favor of charge transfer and extraction.Furthermore,the PEA additive can efficiently block moisture and oxygen to prolong the device lifetime.As a result,PEA-treated MAPbI3(MA:CH3NH3)solar cells show increased power conversion efficiency(PCE)(from 17.18 to 18.87%)and good longterm stability.When PEA is introduced to(FAPbI3)1-x(MAPbBr3)x(FA:HC(NH2)2)solar cells,the PCE is enhanced from 19.66 to 21.60%.For both perovskites,their severe device hysteresis is efficiently relieved by PEA. | Ningli Chen Xiaohui Yi Jing Zhuang Yuanzhi Wei Yanyan Zhang Fuyi Wang Shaokui Cao Cheng Li Jizheng Wang | 2020 | Nano-Micro Letters2020，12，12： | 0 |
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