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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | ShHTL7 is a non-canonical receptor for strigolactones in root parasitic weeds显示文摘 | Yao, Ruifeng Wang, Fei Ming, Zhenhua Du, Xiaoxi Chen, Li Wang, Yupei Zhang, Wenhao Deng, Haiteng Xie, Daoxin | 2017 | Cell Research2017，27，6： | 15 |
| 3 | Effect of miniscalpel-needle on relieving the pain of myofascial pain syndrome: a systematic review显示文摘OBJECTIVE: To evaluate the effect and safety of miniscalpel-needle(MSN) on reducing the pain of myofascial pain syndrome(MPS).METHODS: We reviewed the available literatures inception up to February 2014 using Pubmed, EMBASE, Cochrane Library, Chinese National Knowledge Infrastructure Database, Chinese Biomedical Database and Wanfang Database.RESULTS: Eight randomized controlled trials were finally identified. The main controls involved acupuncture, medications, injection, massage and cupping. We found that all of the studies agreed on the potential benefit of MSN as a strategy for MPS and the superiority compared to the controls, however,randomized methods applied in most of the trials could be criticized for their high or unclear risk of bias. Further research is also needed to clarify questions around the appropriate frequency and number of treatment sessions of MSN.CONCLUSION: This review shows that MSN might have the effect on MPS, even though there were some limitations in the studies included in the review. Studies with robust methodology are warranted to further test its pain-relieving effect on MPS. | Liu Tong Peng Yuanyuan Zhu Shipeng Chen Huan Li Fuyun Hong Peixin Cao Bingyan Peng Bo Fan Yifan Chen Yupei Zhang Li | 2015 | Journal of Traditional Chinese Medicine2015，35，6： | 6 |
| 4 | A PIF7-CONST ANS-Centered Molecular Regulatory Network Underlying Shade-Accelerated Flowering显示文摘To compete with their neighbors for light and escape shaded environments,sun-loving plants have devel-oped the shade-avoidance syndrome(SAS),a set of responses including alteration of plant architecture and initiation of early flowering and seed set.Previous studies on SAS mainly focused on dissecting molec-ular basis of hypocotyl elongation in seedlings under shade light;however,the molecular mechanisms underlying shade-accelerated flowering in adult plants remain unknown.In this study,we found that CONSTANS(CO)and PHYTOCHROME-INTERACTING FACTOR 7(PIF7)have an additive effect on shade-induced flowering,but that LONG HYPOCOTYL IN FAR-RED1(HFR1)represses early flowering by binding to CO and PIF7 and preventing the binding of CO to the promoter of FLOWERING LOCUS T(FT)and the binding of PIF7 to the promoter of pri-MIR156E/F.Under shade,de-phosphorylated PIF7 and accumulated CO,balanced by HFR1,upregulate the expression of FT,TSF,SOC1,and SPLs to accelerate flowering.Moreover,we found that the function of PIF7 in flowering time is independent of phyA.Collectively,these regulatory interactions establish a crucial link between the light signal and genetic network that regulates flowering transition under shade. | Renshan Zhang Chuanwei Yang Yupei Jiang Lin Li | 2019 | Molecular Plant2019，12，12： | 5 |
| 5 | Effects of Electrode on Resistance Switching Properties of ZnMn_2O_4 Films Deposited by Magnetron Sputtering显示文摘ZnMn_2O_4 films for resistance random access memory(RRAM) were fabricated with different device structures by magnetron sputtering. The effects of electrode on I-V characteristics, resistance switching behavior, endurance and retention characteristics of ZnMn_2O_4 films were investigated. The ZnMn_2O_4 films, using p-Si and Pt as bottom electrode, exhibit bipolar resistive switching(BRS) behavior dominated by the space-charge-limited conduction(SCLC) mechanism in the high resistance state(HRS) and the filament conduction mechanism in the low resistance state(LRS), but the ZnMn_2O_4 films using n-Si as bottom electrodes exhibit both bipolar and unipolar resistive switching behaviors controlled by the Poole-Frenkel(P-F) conduction mechanism in both HRS and LRS. Ag/ZnMn_2O_4/p-Si device possesses the best endurance and retention characteristics, in which the number of stable repetition switching cycle is over 1000 and the retention time is longer than 106 seconds. However, the highest RHRS/R_(LRS) ratio of 104 and the lowest V_(ON) and V_(OFF) of 3.0 V have been observed in Ag/ZnMn_2O_4/Pt device. Though the Ag/ZnMn_2O_4/n-Si device also possesses the highest RHRS/R_(LRS) ratio of 104, but the highest values of V_(ON),V_(OFF), RHRS and R_(LRS), as well as the poor endurance and retention characteristics. | 王华 li zhida xu jiwen zhang yupei yang ling | 2016 | Journal of Wuhan University of Technology(Materials Science)2016，31，6： | 2 |
| 6 | Yes-associated protein (YAP65) in relation to Smad7 expression in humanpancreatic ductal adenocarcinoma显示文摘 | Junchao Guo J?rg Kleeff Yupei Zhao Junsheng Li Thomas Giese Irene Esposito Markus Büchler Murray Korc Helmut Friess | 2006 | International Journal of Molecular Medicine2006，，5： | 2 |
| 7 | Selective potassium uptake via biocompatible zeolite–polymer hybrid microbeads as promising binders for hyperkalemia显示文摘Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with low curative effects and various side effects.Herein,direct serum potassium uptake was realized via zeolite–heparin-mimicking-polymer hybrid microbeads.The preparation process involved the synthesis of the heparin-mimicking polymer via the in situ cross-linking polymerization of acrylic acid and N-vinylpyrrolidone in polyethersulfone solution,the fabrication of microbeads via zeolite-mixing,electro-spraying and phase-inversion,and the subsequent aqueous-phase modifications based on ion-exchange and metal-leaching.An ultra-high(about 88%)amount of zeolite could be incorporated and well locked inside the polymer matrix.Potassium uptake capability was verified in water,normal saline and human serum,showing high selectivity and fast adsorption.The microbeads exhibited satisfying blood compatibility,negligible hemolysis ratio,prolonged clotting time,inhibited contact activation,and enhanced antifouling property toward serum proteins and cells.The proposed approach toward zeolite–heparin-mimicking-polymer hybrid microbeads provided a cheap,efficient and safe treatment protocol of hyperkalemia for the high-risk patients. | Zhoujun Wang Wei Sun Zhiwei Wei Jianxu Bao Xin Song Yupei Li Haifeng Ji Jue Zhang Chao He Baihai Su Weifeng Zhao Changsheng Zhao | 2021 | Bioactive Materials2021，6，2： | 2 |
| 8 | Increased COMT expression in pancreatic cancer and correlation with clinicopathologic parameters显示文摘Catechol-O-methyl transferase (COMT) is an enzyme involved in estrogen metabolism. Proteomic and immunoproteomic screens suggested COMT might be an immunogenic membrane antigen in human pancreatic cancer. The aim of this study was to investigate the dynamic expression of COMT in pancreatic ductal adenocarcinoma (PDAC) and noncancerous pancreatic tissue, and to determine the relationship between COMT expression and clinicopathologic parameters. COMT expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and western blot in five pancreatic cell lines and five pairs of PDAC and noncancerous pancreatic tissue. Immunohistochemistry was used to evaluate COMT expression in tissue microarrays and 20 cases of paraffin-embedded clinical specimens. The results indicated that COMT expression was detected in AsPC-1, BxPC-3, MIA PaCa-2, Capan-1 and SW1990 pancreatic cell lines, and in five pairs of PDAC and noncancerous pancreatic tissue, at the mRNA and protein levels. Immunohistochemistry analysis revealed that COMT expression was significantly higher in PDAC than in nonmalignant pancreatic tissue. High expression of COMT significantly correlated to early T stages. Therefore, we conclude that COMT might serve as a potential biomarker for applied clinical pathology in PDAC. | WU WenMing ZHANG Jie ZHOU Li YOU Lei ZHAO YuPei LI Ji | 2012 | Science China(Life Sciences)2012，55，9： | 2 |
| 9 | Targeting the Neddylation Pathway to Suppress the Growth of Prostate Cancer Cells: Therapeutic Implication for the Men’s Cancer显示文摘 | Xiaofang Wang Lihui Li Yupei Liang Chunjie Li Hu Zhao Dingwei Ye Menghong Sun Lak Shin Jeong Yan Feng Shen Fu Lijun Jia Xiaomao Guo Zhen Chen | 2014 | BioMed Research International2014，，： | 1 |
| 10 | Phase separation of the nuclear pore complex facilitates selective nuclear transport to regulate plant defense against pathogen and pest invasion显示文摘The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to dominate the nucleocytoplasmic trafficking essential for many important signaling events in yeast and mammals.How plant NPC central barrier controls selective transport is a crucial question remaining to be elucidated.In this study,we uncovered that phase separation of the central barrier is critical for the permeability and selectivity of plant NPC in the regulation of various biotic stresses.Phenotypic assays of nup62 mutants and complementary lines showed that NUP62 positively regulates plant defense against Botrytis cinerea,one of the world’s most disastrous plant pathogens.Furthermore,in vivo imaging and in vitro biochemical evidence revealed that plant NPC central barrier undergoes phase separation to regulate selective nucleocytoplasmic transport of immune regulators,as exemplified by MPK3,essential for plant resistance to B.cinerea.Moreover,genetic analysis demonstrated that NPC phase separation plays an important role in plant defense against fungal and bacterial infection as well as insect attack.These findings reveal that phase separation of the NPC central barrier serves as an important mechanism to mediate nucleocytoplasmic transport of immune regulators and activate plant defense against a broad range of biotic stresses. | Jiaojiao Wang Gaofeng Pei Yupei Wang Dewei Wu Xiaokang Liu Gaoming Li Jianfang He Xiaolin Zhang Xiaoyi Shan Pilong Li Daoxin Xie | 2023 | Molecular Plant2023，16，6： | 1 |
| 11 | Resistance Switching Properties of Ag/Zn Mn_2O_4/p-Si Fabricated by Magnetron Sputtering for Resistance Random Access Memory显示文摘有 Ag/ZnMn 2 O 4/p-Si 被磁控管劈啪作响制作方法。可靠、重复 ZnMn 2 O 4 电影与狭窄的分散和 3V 切换电压在高、低的电阻的二个明确的状态之间被获得。高电阻状态和低电阻状态的电阻比率在约 10 个 3 数量级并且直到大约 10 个 3 测试周期的范围被发现。Ag/ZnMn 2 O 4/ 希腊语的第二十三个字母设备比 10 6 秒长,在二之间的电阻比率在房间温度比 10 3 高说遗体,为 nonvolatile 存储器申请显示出 RRAM 设备的显著可靠性表演。为 HRS (高电阻状态) 和 LRS (低电阻状态) 的相等的模拟电路被阻抗光谱学也学习。 | 王华 LI Zhida XU Jiwen ZHANG Yupei YANG Ling QIU Wei | 2015 | Journal of Wuhan University of Technology(Materials Science)2015，30，6： | 1 |
| 12 | Guidelines for the diagnosis and treatment of acute pancreatitis in China (2021)显示文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China. | Fei Li Shouwang Cai Feng Cao Rufu Chen Deliang Fu Chunlin Ge Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Ang Li Haimin Li Shengping Li Weiqin Li Yixiong Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Changqing Yan Yinmo Yang Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 1 |
| 13 | Author Correction: 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘In the initial published version of this article, there was a mistake in the P value for the correlation between gene-expression changes and 5 hmC changes in tumors. The correct P value should be same as the P value shown in Fig. S6A: 9.8?×?10?6 (mistakenly shown as “9.8?×?106” in the main text). This correction does not affect the description of results or the conclusions of this study, since the range of P value is between 0 and 1. | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2019 | Cell Research2019，29，7： | 1 |
| 14 | Effects of extracts from soothing-liver and invigorating-spleen formulas on the injury induced by oxidative stress in the hepatocytes of rats with non-alcoholic fatty liver disease induced by high-fat diet显示文摘OBJECTIVE:To investigate effects of the extracts from soothing-liver and invigorating-spleen formulas on the injury due to oxidative stress,mediated by the Nuclear factor-like 2(Nrf2)-Antioxidant response element(ARE) pathway,in the hepatocytes of rats with non-alcoholic fatty liver disease(NAFLD) induced by high-fat diet.METHODS:Soothing-liver and invigorating-spleen formula mixtures were prepared for five groups:normal,model,soothing-liver formula group(SLG),invigorating-spleen formula group(ISG),integrated formula group(IG).The rat model of NAFLD was induced by feeding rats a high-fat diet(HFD).After 16 weeks,the hepatic tissue was examined following Haematoxylin-Eosin(H&E) staining and with Transmission electron microscopy(TEM).Levels of hepatic lipids,serum lipids and hepatic functions were measured using a biochemical analyser.Hepatocytes were isolated from the livers of rats and were identified by cellar immunohistochemistry,cellular immunofluorescence and flow cytometry.The expression levels of Nrf2,Kelch-like epichlorohydrin-associated protein 1(Keap-1),haeme oxygenase-1(HO-1) and NAD(P)H:quinone oxidoreductase 1(NQO1) m RNAs were assessed by real-time fluorescence quantitative PCR.Nrf2,Keap-1,HO-1 and NQO1 proteins were measured by Western blotting.Finally,the levels of oxidative stress factors Superoxide Dismutase(SOD),malonaldehyde(MDA) and Glutathione peroxidase(GSH-Px) in hepatocytes were measured by WST-1,TBA and colorimetry.RESULTS:The H & E and TEM results showed that the NAFLD model rats successfully reproduced typical pathogenetic and histopathological features.The liver function and levels of hepatic lipids and serum lipids from the model rats were dramatically increased.Compared with the model group,the levels of hepatic lipids,serum lipids and hepatic function in the treatment groups were ameliorated to different degrees.The yields of purified hepatocytes in each rat were 4-5 × 108.The viability of the isolated hepatocytes was higher than 95%,with a purity over 93.2%.Cellular immunohistochemistry analysis showed that the hepatocytes were brown,while in the cellular immunofluorescence analysis,the hepatocytes showed green fluorescence.The expression levels of Nrf2,Keap-1,HO-1 and NQO1 m RNA and protein in the hepatocytes were significantly higher in the model group than in the normal group(P < 0.05,P < 0.01).Compared with the model group,the expression of Nrf2,Keap-1,HO-1 and NQO-1 m RNAs and proteins in all treatment groups increased,especially in the IG(P < 0.01).CONCLUSION:The extracts from soothing-liver and invigorating-spleen formulas may protect the liver against the injury induced by oxidative stress in hepatocytes by influencing the Nrf2-ARE pathway,which may be the mechanism having the potential for prevention and treatment of NAFLD. | Tu Guifang Gong Xiangwen Yang Qinhe Li Yuanyuan Wang Guanlong Liang Yinji Zhang Yupei Yan Haizhen Lin Chunmei Zhang Jinwen | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 1 |
| 15 | A breakthrough trial of an artificial liver without systemic heparinization in hyperbilirubinemia beagle models显示文摘The development of wearable artificial livers was restricted to device miniaturization and bleeding risk with water-soluble anticoagulants.Herein,a double-deck column filled with solid anticoagulant microspheres and Kevlar porous microspheres(KPMs,bilirubin adsorbents)was connected with the principle machine of wearable artificial liver(approximately 9 kg)to treat hyperbilirubinemia beagles for the first time.With the initial normal dose of heparin,the double-deck column could afford 3 h hemoperfusion in whole blood without thrombus formation.The removal efficiency of the double-deck column for total bilirubin(TBIL)was 31.4%.Interestingly,the excessive amounts of hepatocyte metabolites were also decreased by approximately 25%.The“anticoagulant+column”realized safe and effective whole blood hemoperfusion without the plasma separation system and heparin pump;however,the proposed principle machine of wearable artificial liver and“anticoagulant+column”cannot completely replace the bio-liver now.The intelligence of the device and the versatility of the adsorbent need to be improved;moreover,advanced experimental techniques need to be developed to validate the survival rates in animals.Overall,this study is a meaningful trial for the development of wearable artificial livers in the future. | Yilin Wang Shanshan Wang Xueqin He Yupei Li Tao Xu Lin Xu Bo Yang Xinnian Fan Weifeng Zhao Changsheng Zhao | 2023 | Bioactive Materials2023，，2： | 0 |
| 16 | Privacy-Preserving Incentive Mechanism for Platoon Assisted Vehicular Edge Computing with Deep Reinforcement Learning显示文摘Platoon assisted vehicular edge computing has been envisioned as a promising paradigm of implementing offloading services through platoon cooperation.In a platoon,a vehicle could play as a requester that employs another vehicles as performers for workload processing.An incentive mechanism is necessitated to stimulate the performers and enable decentralized decision making,which avoids the information collection from the performers and preserves their privacy.We model the interactions among the requester(leader)and multiple performers(followers)as a Stackelberg game.The requester incentivizes the performers to accept the workloads.We derive the Stackelberg equilibrium under complete information.Furthermore,deep reinforcement learning is proposed to tackle the incentive problem while keeping the performers’information private.Each game player becomes an agent that learns the optimal strategy by referring to the historical strategies of the others.Finally,numerical results are provided to demonstrate the effectiveness and efficiency of our scheme. | Xumin Huang Yupei Zhong Yuan Wu Peichun Li Rong Yu | 2022 | China Communications2022，19，7： | 0 |
| 17 | Consensus of clinical diagnosis and treatment for non-functional pancreatic neuroendocrine neoplasms with diameter<2 cm显示文摘In clinical practice,pancreatic neuroendocrine neoplasms(pNENs)with a diameter smaller than 2 cm are commonly referred to as small pNENs.Due to their generally favorable biological characteristics,the diagnosis and treatment of small pNENs differ from other pNENs and are somewhat controversial.In response to this,the Chinese Pancreatic Surgery Association,Chinese Society of Surgery,Chinese Medical Association have developed a consensus on the diagnosis and treatment of small pNENs,which is based on evidence-based medicine and expert opinions.This consensus covers various topics,including concepts,disease assessment,treatment selection,follow-up,and other relevant aspects. | Wu Wenming Cai Shouwang Chen Rufu Fu Deliang Ge Chunlin Hao Chunyi Hao Jihui Huang Heguang Jian Zhixiang Jin Gang Li Fei Li Haimin Li Shengping Li Weiqin LiYixiong Liang Tingbo Liu Xubao Lou Wenhui Miao Yi Mou Yiping Peng Chenghong Qin Renyi Shao Chenghao Sun Bei Tan Guang Wang Huaizhi Wang Lei Wang Wei Wang Weilin Wei Junmin Wu Heshui Wu Zheng Yan Changqing Yang Yinmo Yin Xiaoyu Yu Xianjun Yuan Chunhui Zhao Yupei | 2023 | Journal of Pancreatology2023，6，3： | 0 |
| 18 | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility显示文摘Cellular behaviors and functions can be regulated by mechanical cues from microenvironments,which are transmitted to nucleus through the physical connections of cytoskeletons in the cells.How these physical connections determine transcriptional activity were not clearly known.The actomyosin,which generates intracellular traction force,has been recognized to control the nuclear morphology.Here,we have revealed that microtubule,the stiffest cytoskeleton,is also involved in the process of nuclear morphology alteration.The microtubule negatively regulates the actomyosin-induced nuclear invaginations but not the nuclear wrinkles.Moreover,these nuclear shape changes are proven to mediate the chromatin remodeling,which essentially mediates cell gene expression and phenotype determination.The actomyosin disruption leads to the loss of chromatin accessibility,which can be partly recovered by microtubule interference through nuclear shape control.This finding answers the question of how mechanical cues regulate chromatin accessibility and cell behaviors.It also provides new insights into cell mechanotransduction and nuclear mechanics. | Jiwen Geng Zhefeng Kang Qian Sun Man Zhang Peng Wang Yupei Li Jiameng Li Baihai Su Qiang Wei | 2023 | Research2023，，3： | 0 |
| 19 | Digestive tract reconstruction in pancreaticoduodenectomy in University Hospitals of China: a national questionnaire survey显示文摘Background Pancreaticoduodenectomy(PD)has been widely applied in general hospitals in China;however,there is still a lack of unified standards for each surgical technique and procedure.This survey is intended to investigate the current status of digestive tract reconstruction after PD in university hospitals in China.Method A cross-sectional survey was conducted among the members of the Young Elite Pancreatic Surgery Club of China by using the Questionnaire for Digestive Tract Reconstruction after Pancreaticoduodenectomy.The questionnaire was disseminated and collected by point-to-point communication via WeChat public platforms.Results A total of 73 valid questionnaires were returned from 65 university hospitals in 28 provincial divisions of China's Mainland.The respondents who performed PD surgery with an annual volume of over 100 cases accounted for 63%.Generally,laparoscopic PD was performed less often than open PD.Child and Whipple reconstructions accounted for 70%and 26%,respectively.The sequence of pancreatoenteric,biliary-enteric,and gastrointestinal reconstruction accounted for 84%of cases.In pancreatoenteric anastomosis,double-layer anastomosis is the most commonly employed type,accounting for approximately 67%,while single-layer anastomosis accounts for 30%.Of the double-layer anastomoses,duct-to-mucosa/dunking(94%/4%)PJ was performed with duct-mucosa using the Blumgart method(39%)and Cattel-Warren(29%),with continuous/interrupted sutures in the inner layer(69%/31%)and continuous/interrupted sutures in the outer layer(53%/23%).In single-layer anastomosis,continuous/interrupted sutures accounted for 41%/45%.In hepatojejunostomy,single-layer/double-layer suture accounted for 79%/4%,and continuous/interrupted suture accounted for 75%/9%.Forty-six percent of the responding units had not applied double-layer biliary-intestinal anastomosis in the last 3 years,75%of the responding surgeons chose the anastomosis method according to bile duct diameter,with absorbable/non-absorbable suture accounting for 86%/12%.PD/pylorus-preserving PD accounted for 79%/11%of GJ cases,the distance between GJ and HJ<30 cm,30-50 cm and>50 cm were 11%,75%,and 14%,respectively.Antecolic/retrocolic GJ accounted for 71%/23%of cases.Twenty-two percent of GJ cases employed Braun anastomosis,while 55%and 19%of GJ cases used linear cutting staplers/tube-type staplers,respectively;60%/14%were reinforced/not reinforced via manual suturing after stapler anastomosis.Manual anastomosis in GJ surgery employed absorbable/non-absorbable sutures(91%/9%).Significant differences in reconstruction techniques were detected between different volumes of PD procedures(<100/year and>100/year),regions with different economic development levels,and between north and south China.Conclusion Digestive tract reconstruction following PD exists heterogeneity in Chinese university hospitals.Corresponding prospective clinical studies are needed to determine the consensus on pancreatic surgery that meets the clinical reality in China. | Jishu Wei Qiang Xu Yuhua Zhang Jiabin Jin Xiaodong Tian Qiaofei Liu Zipeng Lu Zheng Wang Shanmiao Gou Song Gao Xianlin Han Yefei Rong Niandong Ji Ye Lin Guolin Li Shi Chen Feng Cao Hua Chen Wenming Wu Yupei Zhao the Young Elite Pancreatic Surgery Club of China | 2022 | Journal of Pancreatology2022，5，3： | 0 |
| 20 | Novel technologies in cfDNA analysis and potential utility in clinic显示文摘The profiling of plasma cell-free DNA(cfDNA)is becoming a valuable tool rapidly for tumor diagnosis,monitoring and prognosis.Diverse plasma cfDNA technologies have been in routine or emerging use,including analyses of mutations,copy number alterations,gene fusions and DNA methylation.Recently,new technologies in cfDNA analysis have been developed in laboratories,and potentially reflect the status of epigenetic modification,the immune microenvironment and the microbiome in tumor tissues.In this review,the authors discuss the principles,methods and effects of the current cfDNA assays and provide an overview of studies that may inform clinical applications in the near future. | Jie Li Mengyue Xu Junya Peng Jingqiao Wang Yupei Zhao Wenming Wu Xun Lan | 2021 | Chinese Journal of Cancer Research2021，33，6： | 0 |
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