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| 1 | microRNA-218 suppresses the proliferation, invasion and promotes apoptosis of pancreatic cancer cells by targeting HMGB1显示文摘Objective: To detect the expression profiles of micro RNA-218(mi R-218) in human pancreatic cancer tissue(PCT) and cells and their effects on the biological features of human pancreatic cancer cell line PANC-1 and observe the effect of mi R-218 on the expression of the target gene high mobility group box 1(HMGB1), with an attempt to provide new treatment methods and strategies for pancreatic cancer.Methods: The expressions of mi R-218 in PCT and normal pancreas tissue as well as in various pancreatic cancer cell lines including As PC-1, Bx PC-3, and PANC-1 were determined with quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR). The change of mi R-218 expression in PANC-1 cells was detected using qR T-PCT after the transfection of miR-218 mimic for 48 h. Cell Counting Kit-8(CCK-8) was applied for detecting the effect of mi R-218 on the activity of PANC-1 cells. The effects of mi R-218 on the proliferation and apoptosis of PANC-1 cells were analyzed using the flow cytometry. The effect of mi R-218 on the migration of PANC-1 cells was detected using the Trans-well migration assay. The HMGB1 was found to be a target gene of mi R-218 by luciferase reporter assay, and the effect of mi R-218 on the expression of HMGB1 protein in cells were determined using Western blotting.Results: As shown by q RT-PCR, the expressions of mi R-218 in PCT and in pancreatic cancer cell line significantly decreased when compared with the normal pancreatic tissue(NPT)(P<0.01). Compared with the control group, the miR-218 expression significantly increased in the PANC-1 group after the transfection of mi R-218 mimic for 48 h(P<0.01). Growth curve showed that the cell viability significantly dropped after the overexpression of mi R-218 in the PANC-1 cells for two days(P<0.05). Flow cytometry showed that the S-phase fraction significantly dropped after the overexpression of mi R-218(P<0.01) and the percentage of apoptotic cells significantly increased(P<0.01). As shown by the Trans-well migration assay, the enhanced mi R-218 expression was associated with a significantly lower number of cells that passed through a Transwell chamber(P<0.01). Luciferase reporter assay showed that, compared with the control group, the relative luciferase activity significantly decreased in the mi R-218 mimic group(P<0.01). As shown by the Western blotting, compared with the control group, the HMGB1 protein expression significantly decreased in the PANC-1 group after the transfection of mi R-218 mimic for 48 h(P<0.01).Conclusions: The mi R-218 expression decreases in human PCT and cell lines. mi R-218 can negatively regulate the HMGB1 protein expression and inhibit the proliferation and invasion of pancreatic cancer cells. A treatment strategy by enhancing the mi R-218 expression may benefit the patients with pancreatic cancer. | Zhe Liu Yuanhong Xu Jin Long Kejian Guo Chunlin Ge Ruixia Du | 2015 | Chinese Journal of Cancer Research2015，27，3： | 7 |
| 2 | Thick Miocene eolian deposits on the Huajialing Mountains:The geomorphic evolution of the western Loess Plateau显示文摘The geomorphic evolution of northwestern China during the Cenozoic has been a subject of much geological interest because of its link with the uplift of the Himalayan-Tibetan complex.Much information about these changes is recoverable from the sedimentary sequences of the region.We report here on the thick eolian deposits mantling the Huajialing Mountains,a relatively flat mountain range in the western Loess Plateau.Correlation of magnetic susceptibility stratigraphy with the QA-I Miocene eolian sequence dates a 134.7 m section(NL-VII) for the interval from 18.7 to 11.8 Ma,as confirmed by micro-mammalian fossils.These eolian deposits demonstrate a much wider distribution of the Miocene eolian deposits,and also indicate that the topography contrasts in the western Loess Plateau,including the uplifts of the Huajialing Mountains and the bedrock highlands in the Qinan region,were formed by the early Miocene.The near-continuous Miocene eolian sequence from 18.7 to 11.8 Ma indicates that the substratum of Huajialing had not experienced any intense tectonic changes during this time interval,which suggests further,the relative tectonic stability of the nearby Tibetan Plateau. | ZHAN Tao GUO ZhengTang WU HaiBin GE JunYi ZHOU Xin WU ChunLin ZENG FangMing | 2011 | Science China Earth Sciences2011，54，2： | 7 |
| 3 | microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression显示文摘Objective: The purpose of this study was to examine the effect of gemcitabine(GEM) on micro RNA-218(mi R-218) expression in human pancreatic cancer cells.Methods: Quantitative reverse transcription polymerase chain reaction(q RT-PCR) was performed to examine the differences in mi R-218 expression between the GEM-sensitive Bx PC-3 pancreatic cancer cells and GEM-resistant PANC-1 cells. The effect of GEM on the expression of mi R-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with HMGB1 si RNA to knock down the expression of HMGB1 or with the recombinant HMGB1 expression vector(pc DNA3.1-HMGB1) to overexpress HMGB1. The effect of ectopic expression of HMGB1 on the apoptosis of mi R-218-transfected and GEMtreated PANC-1 cells was examined by flow cytometric analysis.Results: The mi R-218 expression level was lower in GEM-resistant PANC-1 cells compared to GEMsensitive Bx PC-3 cells(P<0.05). The percentage of apoptotic PANC-1 cells was significantly increased in the mi R-218 mimic + GEM group compared to the mimic ctrl + GEM group and the normal control group(P<0.01). The HMGB1 expression level was markedly decreased in PANC-1 cells transfected with HMGB1 si RNA but was significantly increased in PANC-1 cells transfected with the recombinant HMGB1 expression vector, pc DNA3.1-HMGB1(P<0.01). The proportion of apoptotic PANC-1 cells was significantly lower in the mi R-218 mimic + GEM + pc DNA3.1-HMGB1 group compared to the mi R-218 mimic + GEM + HMGB1 si RNA group(P<0.01).Conclusions: The expression level of mi R-218 was downregulated in the GEM-resistant cell line. mi R-218 promoted the sensitivity of PANC-1 cells to GEM, which was achieved mainly through regulating the expression of HMGB1 in PANC-1 cells. | Zhe Liu Ruixia Du Jin Long Kejian Guo Chunlin Ge Shulong Bi Yuanhong Xu | 2015 | Chinese Journal of Cancer Research2015，27，3： | 2 |
| 4 | High resolution estimates of paleo-CO 2 levels through the Campanian (Late Cretaceous) based on Ginkgo cuticles显示文摘 | Cheng Quan Chunlin Sun Yuewu Sun Ge Sun | 2008 | Cretaceous Research2008，，2： | 1 |
| 5 | Sedimentological features of Cretaceous--Tertiary (K/T) boundary section along Heilongjiang River,Northeast China显示文摘The Jiayin area along the Heilongjiang River in Northeast China has attracted stratigraphic and sedimentological interests recently because the Cretaceous--Tertiary (K/T) boundary should be located in this non-marine succession.Dinosaur remains disappear suddenly at the upper part of the Maastrichtian,whereas plants gradually evolved throughout the succession.Analysis of sedimentary facies has been carried out and paleo-environments through Upper Cretaceous to lower Paleocene sections are reconstructed.Fluvial to lacustrine sedimentary environments are inferred from the facies.Several debris flow events have been identified,and it is in these deposits that the dinosaur fossils are concentrated. | Shigeyuki Suzuki Hakuyu Okada SUN Chunlin SUN Ge | 2010 | Global Geology2010，13，1： | 1 |
| 6 | A decision support system for irrigation water allocation along the middle reaches of the Heihe River Basin,Northwest China显示文摘 | Ge Yingchun Li Xin Huang Chunlin | 2013 | Environmental Modelling&Software2013，47，： | 1 |
| 7 | A Decision Support System for irrigation water allocation along the middle reaches of the Heihe River Basin, Northwest China显示文摘 | Yingchun Ge Xin Li Chunlin Huang Zhuotong Nan | 2013 | Environmental Modelling and Software2013，，： | 1 |
| 8 | Guidelines for the diagnosis and treatment of acute pancreatitis in China (2021)显示文摘Acute pancreatitis(AP)is a common acute abdominal condition of the digestive system.In recent years,treatment concepts,methods,and strategies for the diagnosis of AP have advanced,and this has played an important role in promoting the standardization of AP diagnosis and treatment and improving the treatment quality of AP patients.On the basis of previous guidelines and expert consensus,this guideline adopts an evidence-based,problem-based expression;synthesizes important clinical research data at home and abroad in the most recent 5 years;and forms 29 recommendations through multidisciplinary expert discussion,including diagnosis,treatment,and follow-up.It is expected to provide evidence support for the treatment of AP in the clinical setting in China. | Fei Li Shouwang Cai Feng Cao Rufu Chen Deliang Fu Chunlin Ge Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Ang Li Haimin Li Shengping Li Weiqin Li Yixiong Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Changqing Yan Yinmo Yang Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 1 |
| 9 | Contribution of non-Saccharomyces yeasts to aroma-active compound production,phenolic composition and sensory profile in Chinese Vidal icewine显示文摘To acquire candidate non-Saccharomyces strains for preparation of high-quality Vidal icewine,11 representative indigenous non-Saccharomyces strains were selected for analyzing their production of organic acids,phenolic compounds,aroma-active compounds and evaluating their sensory profile in Vidal juice.The results showed that the contents of organic acids in the wines were controlled and balanced well by the non-Saccharomyces strains as well as polyphenol concentrations were well improved during the fermentation.The strains differed from each other in their ability to produce aroma compounds,causing diversity and complexity of aroma characteristics in wine.Fermentation by Saccharomycopsis crataegensis YC30(Sc)and Hanseniaspora uvarum QTX22(Hu)could enhance the sweet and floral notes in Vidal icewine,especially Sc,which has never been reported before.It can not only enrich the aroma categories,but also increase the content ofδ-decalactone,γ-decalactone,ethyl isobutyrate,ethyl butyrate,phenethyl acetic acid,etc.The evaluation and optimization of non-Saccharomyces yeast strains could provide theoretical basis for the improvement of ice wine quality,and supply new ideas and strategies for the brewing of ice wine. | Qian Ge Chunfeng Guo Yue Yan Xiangyu Sun Tingting Ma Jing Zhang Caihong Li Chunlin Gou Tianli Yue Yahong Yuan | 2022 | Food Bioscience2022，46，2： | 1 |
| 10 | Association of sonographically detected calcification with thyroid carcinoma 显示文摘 | Wang Ning Xu Yuanhong Ge Chunlin | 2006 | Head Neck2006，28，12： | 1 |
| 11 | A Serum Biomarker Model to Diagnose Pancreatic Cancer Using Proteomic Fingerprint Technology显示文摘OBJECTIVE To establish a serum protein pattern model for screening pancreatic cancer. METHODS Twenty-nine serum samples from patients with pancreatic cancer were collected before surgery,and an additional 57 serum samples from age and sex-matched individuals without cancer were used as controls.WCX magnetic beans and a PBS Ⅱ-C protein chip reader (Ciphergen Biosystems Inc) were employed to detect the protein fingerprint expression of all serum samples. The resulting profiles comparing serum from cancer and normal patients were analyzed with the Biomarker Wizard system,to establish a model using the Biomarker Pattern system software. A double-blind test was used to determine the sensitivity and specificity of the model. RESULTS A group of 4 biomarkers(relative molecular weights were 5,705 Da,4,935 Da,5,318 Da,3,243 Da) were selected to set up a decision tree to produce the classification model to effectively screen pancreatic cancer patients.The results yielded a sensitivity of 100%(20/20),specificity of 97.4% (37/38).The ROC curve was 99.7%.A double-blind test used to challenge the model resulted in a sensitivity of 88.9% and a specificity of 89.5%. CONCLUSION New serum biomarkers of pancreatic cancer have been identified.The pa ern of combined markers provides a powerful and reliable diagnostic method for pancreatic cancer with high sensitivity and specificity. | Chunlin Ge Ning Ma Dianbo Yao Fengming Luan Chaojun Hu Yongzhe Li Yongfeng Liu | 2008 | Chinese Journal of Clinical Oncology2008，5，3： | 0 |
| 12 | Consensus of clinical diagnosis and treatment for non-functional pancreatic neuroendocrine neoplasms with diameter<2 cm显示文摘In clinical practice,pancreatic neuroendocrine neoplasms(pNENs)with a diameter smaller than 2 cm are commonly referred to as small pNENs.Due to their generally favorable biological characteristics,the diagnosis and treatment of small pNENs differ from other pNENs and are somewhat controversial.In response to this,the Chinese Pancreatic Surgery Association,Chinese Society of Surgery,Chinese Medical Association have developed a consensus on the diagnosis and treatment of small pNENs,which is based on evidence-based medicine and expert opinions.This consensus covers various topics,including concepts,disease assessment,treatment selection,follow-up,and other relevant aspects. | Wu Wenming Cai Shouwang Chen Rufu Fu Deliang Ge Chunlin Hao Chunyi Hao Jihui Huang Heguang Jian Zhixiang Jin Gang Li Fei Li Haimin Li Shengping Li Weiqin LiYixiong Liang Tingbo Liu Xubao Lou Wenhui Miao Yi Mou Yiping Peng Chenghong Qin Renyi Shao Chenghao Sun Bei Tan Guang Wang Huaizhi Wang Lei Wang Wei Wang Weilin Wei Junmin Wu Heshui Wu Zheng Yan Changqing Yang Yinmo Yin Xiaoyu Yu Xianjun Yuan Chunhui Zhao Yupei | 2023 | Journal of Pancreatology2023，6，3： | 0 |
| 13 | Recent advance in study of K-Pg boundary in Jiayin of Heilongjiang,China and its adjacent area显示文摘The non-marine Cretaceous-Paleogene boundary(KPgB)in Jiayin of Heilongjiang was first defined and reported in China by the authors research team in 2011.Thereafter the continuous research on the KPgB and its related Late Cretaceous biota in Jiayin has been made by the authors in 2012-2020.The achievements of the research are mainly reflected as follows:(1)a new drilling borehole with 60 m in depth carried out in the Xiaoheyan of Jiayin in 2016,supplemented new palynological evidence for the KPgB definition in 2011;(2)some radiometric dating newly made on the strata related to the KPgB in Jiayin and its neighboring Russian area,provided the supplemental evidence for the KPgB definition in Jiayin;(3)many new fossils found by the authors,represented by the angiosperms Dalembia and Nelumbo,refresh understanding the Late Cretaceous environment of Jiayin;and(4)the TEM method is applied in the study of pollen exine ultrastructure of Pseudointegricorpus,Wodehouseia,and Aquilapollenites,promoting the late Maastrichtian ecological study in Jiayin,related to the KPgB. | SUN Ge SUN Chunlin SUN Yuewu ZHANG Shuqin WU Wenhao YANG Tao LIANG Fei FENG Yuhui Markevich V Ashraf A R Bugdaeva E. Tekleva M. Suzuki S. | 2021 | Global Geology2021，24，4： | 0 |
| 14 | Degradation Behavior and Safe Application Technology of 30% Kresoxim-methyl SC in Cucumber and Soil显示文摘[Objective] The paper was to determine the residue behavior and environmental safety of 30% kresoxim-methyl SC applied on cucumber under greenhouse conditions. [Method] Referring to Test Standard of Pesticide Residues,the residue dynamics of kresoxim-methyl SC in cucumber was determined by GC.[Result]The half lives of kresoxim-methly in cucumber of Yinchuan,Changsha,Harbin were 2. 8-3. 1,2. 4-3. 7,2. 1-5. 1 d,respectively,and the final residue in cucumber after application for 3,5,7 d was less than 0. 500 0 mg / kg that was the limited standards of the state. [Conclusion]Under the proposed cultivation conditions,it is recommended to use the maximum dosage formulation of 270 g. a. i / ha( preparation 0. 09 g / m2),with spraying up to three times and the safe interval of 3- 5 d. | Ge Qian Gou Chunlin Jiang Rui | 2014 | Plant Diseases and Pests2014，5，6： | 0 |
| 15 | Guidelines for the diagnosis and treatment of pancreatic cancer in China (2021)显示文摘The incidence of pancreatic cancer has been rising worldwide,and its clinical diagnosis and treatment remain a great challenge.To present the update and improvements in the clinical diagnosis and treatment of pancreatic cancer in recent years,Chinese Pancreatic Association,the Chinese Society of Surgery,Chinese Medical Association revised the Guidelines for the Diagnosis and Treatment of Pancreatic Cancer in China(2014)after reviewing evidence-based and problem-oriented literature published during 2015-2021,mainly focusing on highlight issues regarding diagnosis and surgical treatment of pancreatic cancer,conversion strategies for locally advanced pancreatic cancer,treatment of pancreatic cancer with oligo metastasis,adjuvant and neoadjuvant therapy,standardized processing of surgical specimens and evaluation of surgical margin status,systemic treatment for unresectable pancreatic cancer,genetic testing,as well as postoperative follow up of patients with pancreatic cancer.Forty recommendation items were finally proposed based on the above issues,and the quality of evidence and strength of recommendations were graded using the Grades of Recommendation,Assessment,Development,and Evaluation system.This guideline aims to standardize the clinical diagnosis and therapy,especially surgical treatment of pancreatic cancer in China,and further improve the prognosis of patients with pancreatic cancer. | Yinmo Yang Xueli Bai Dapeng Bian Shouwang Cai Rufu Chen Feng Cao Menghua Dai Chihua Fang Deliang Fu Chunlin Ge Xiaochao Guo Chunyi Hao Jihui Hao Heguang Huang Zhixiang Jian Gang Jin Fei Li Haimin Li Shengping Li Weiqin Li Yixiong Li Hongzhen Li Tingbo Liang Xubao Liu Wenhui Lou Yi Miao Yiping Mou Chenghong Peng Renyi Qin Chenghao Shao Bei Sun Guang Tan Xiaodong Tian Huaizhi Wang Lei Wang Wei Wang Weilin Wang Junmin Wei Heshui Wu Wenming Wu Zheng Wu Jingyong Xu Changqing Yan Xiaoyu Yin Xianjun Yu Chunhui Yuan Taiping Zhang Jixin Zhang Jun Zhou Yupei Zhao on behalf of the Chinese Pancreatic Surgery Association | 2021 | Journal of Pancreatology2021，4，2： | 0 |
| 16 | Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells显示文摘BACKGROUND: Microglia are very sensitive to environmental changes, often becoming activated by pathological conditions. Activated microglia can exert a dual role in injury and repair in various diseases of the central nervous system, including cerebral ischemia, Parkinson’s disease, and Alzheimer’s disease. OBJECTIVE: An immortal microglial cell line, BV2, was treated with varying concentrations of lipopolysaccharide (LPS) to induce a pathological situation. Supernatant was harvested and incubated with bone marrow mesenchymal stem cells and, concomitantly, bone marrow mesenchymal stem cell differentiation was observed. DESIGN: A controlled observation, in vitro experiment. SETTING: Department of Neurology, First Affiliated Hospital of China Medical University. MATERIALS: Five male 2–3-week-old Sprague Dawley rats were purchased from Animal Laboratory Center of China Medical University and included in this study. The protocol was performed in accordance with ethical guidelines for the use and care of animals. The microglial cell line BV2 was produced by Cell Research Institute of Chinese Academy of Sciences. LPS was produced by Sigma Company, USA. METHODS: This study was performed in the Central Laboratory of China Medical University from September 2006 to March 2007. Rat femoral and tibial bone marrow was collected for separation and primary culture of bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cell cultures were divided into 5 groups: control group, non-activated group, as well as low-, medium-, and high-dose LPS groups. In the control group, bone marrow mesenchymal stem cells were cultured with Dulbecco’s modified Eagle’s medium (DMEM) supplemented with fetal bovine serum (volume fraction 0.1). In the non-activated group, bone marrow mesenchymal stem cells were incubated with non-activated BV2 supernatant. In the low-, medium-, and high-dose LPS groups, bone marrow mesenchymal stem cells were incubated with LPS (0.01, 0.1 and 1 μg/L, respectively)-activated BV2 supernatant. MAIN OUTCOME MEASURES: Expression of glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) in bone marrow mesenchymal stem cells was detected by immunofluorescence staining. RESULTS: GFAP-positive cells were detected in each group; however, the greatest number were found in the high-dose LPS group. The number of GFAP-positive cells was significantly greater in the high- and medium-dose groups, compared to the control, non-activated, and low-dose LPS groups (P < 0.05). However, there was no significant difference between the medium- and high-dose LPS groups (P > 0.05). NSE-positive cells were also detected in each group. However, there was no significant difference between any two groups (P > 0.05). CONCLUSION: Microglia, when activated to some ertent, could induce neuroglial cell differentiation from bone marrow mesenchymal stem cells; however, they did not exhibit the capacity to markedly promote neuronal cell differentiation. When microglia are activated, the capacity to induce bone marrow mesenchymal stem cell differentiation reaches a peak level, but is not increased with greater activation rates. | Xiaoguang Luo Chunlin Ge Yan Ren Hongmei Yu Zhe Wu Qiushuang Wang Chaodong Zhang | 2008 | Neural Regeneration Research2008，3，3： | 0 |
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