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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Porphyromonas gingivalis exacerbates ulcerative colitis via Porphyromonas gingivalis peptidylarginine deiminase显示文摘Abstract:Ulcerative Colitis(UC)has been reported to be related to Porphyromonas gingivalis(P.gingivalis).Porphyromonas gingivalis peptidylarginine deiminase(PPAD),a virulence factor released by P.gingivalis,is known to induce inflammatory responses.To explore the pathological relationships between PPAD and UC,we used homologous recombination technology to construct a P.gingivalis strain in which the PPAD gene was deleted(Δppad)and aΔppad strain in which the PPAD gene was restored(comΔppad).C57 BL/6 mice were orally gavaged with saline,P.gingivalis,Δppad,or comΔppad twice a week for the entire 40 days(days 0-40),and then,UC was induced by dextran sodium sulfate(DSS)solution for 10 days(days 31-40).P.gingivalis and comΔppad exacerbated DDS-induced colitis,which was determined by assessing the parameters of colon length,disease activity index,and histological activity index,butΔppad failed to exacerbate DDS-induced colitis.Flow cytometry and ELISA revealed that compared withΔppad,P.gingivalis,and comΔppad increased T helper 17(Th17)cell numbers and interleukin(IL)-17 production but decreased regulatory T cells(Tregs)numbers and IL-10 production in the spleens of mice with UC.We also cocultured P.gingivalis,Δppad,or comΔppad with T lymphocytes in vitro and found that P.gingivalis and comΔppad significantly increased Th17 cell numbers and decreased Treg cell numbers.Immunofluorescence staining of colon tissue paraffin sections also confirmed these results.The results suggested that P.gingivalis exacerbated the severity of UC in part via PPAD. | Xida Zhao Jingbo Liu Chong Zhang Ning Yu Ze Lu Shuwei Zhang Yuchao Li Qian Li Junchao Liu Dongjuan Liu Yaping Pan | 2021 | International Journal of Oral Science2021，13，3： | 5 |
| 3 | A New Species of the Genus Thermophis(Serpentes: Colubridae) from Shangri-La, Northern Yunnan, China, with a Proposal for an Eclectic Rule for Species Delimitation显示文摘The hot-spring snakes, Thermophis, were previously known only from the Tibet Autonomous Region and Western Sichuan, China. During the past two years, three adult hot-spring snakes(2 females, 1 male) were sampled in Shangri-La, northern Yunnan, China, thus expanding their known distribution region towards the southeast. This site is the southeastern-most corner of the Tibetan Plateau and the southernmost tip of the Hengduan Mountains(Mts.). Phylogenetic analysis of mitochondrial(mt) and nuclear(n) DNA segments suggested that the three specimens belong to the genus of Thermophis. Morphologically, the new species is more similar to T. zhaoermii. However, it is distinguished from T. zhaoermii in the number of maxillary teeth(15), distance between the two eyes/head width, rostral width/height, mental width/height, in one character limited to female: head width/length, and in four characters restricted to male: occurrence of the reduction from 10 to 8(8 to 6, 6 to 4) scales in each dorsal row on the tail. There are differences in morphology, genetics(mt DNA, n DNA), and geography between the putative new species and T. zhaoermii, the new species meets our proposed eclectic and feasible 'four-differences' rule. | Lifang PENG Changhu LU Song HUANG Peng GUO Yaping ZHANG | 2014 | Asian Herpetological Research2014，5，4： | 4 |
| 4 | Application of next-generation sequencing to screen for pathogenic mutations in 123 unrelated Chinese patients with Marfan syndrome or a related disease显示文摘Marfan syndrome(MFS) is a systemic connective tissue disease principally affecting the ocular, skeletal and cardiovascular systems. This autosomal dominant disorder carries a prevalence of 1:3,000 to 1:5,000. This study aims to define the mutational spectrum of MFS related genes in Chinese patients and to establish genotype-phenotype correlations in MFS. Panel-based targeted next-generation sequencing was used to analyze the FBN1, TGFBR1 and TGFBR2 genes in 123 unrelated Chinese individuals with MFS or a related disease. Genotype-phenotype correlation analyses were performed in mutation-positive patients. The results showed that 97 cases/families(78.9%;97/123) harbor at least one(likely) pathogenic mutation, most of which were in FBN1;four patients had TGFBR1/2 mutations;and one patient harbored a SMAD3 mutation. Three patients had two FBN1 mutations, and all patients showed classical MFS phenotypes. Patients with a dominant negative-FBN1 mutation had a higher prevalence of ectopia lentis(EL). Patients carrying a haploinsufficiency-FBN1 mutation tended to have aortic dissection without EL. This study extends the spectrum of genetic backgrounds of MFS and enriches our knowledge of genotype-phenotype correlations. | Jiacheng Li Chaoxia Lu Wei Wu Yaping Liu Rongrong Wang Nuo Si Xiaolu Meng Shuyang Zhang Xue Zhang | 2019 | Science China(Life Sciences)2019，62，12： | 4 |
| 5 | Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies显示文摘Neutralizing monoclonal antibodies(nAbs)to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)represent promising candidates for clinical intervention against coronavirus disease 2019(COVID-19).We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain(RBD)of the viral spike(S)protein and the receptor angiotensin converting enzyme 2(ACE2).However,the structural basis for their potent neutralizing activity remains unclear.Here,we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2.The bivalent binding of the full-length IgG is found to associate with more RBDs in the“up”conformation than the monovalent binding of Fab,perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein.Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities.This work provides a structural basis for further understanding the mechanism of nAbs,especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit. | Renhong Yan Ruoke Wang Bin Ju Jinfang Yu Yuanyuan Zhang Nan Liu Jia Wang Qi Zhang Peng Chen Bing Zhou Yaning Li Yaping Shen Shuyuan Zhang Long Tian Yingying Guo Lu Xia Xinyue Zhong Lin Cheng Xiangyang Ge Juanjuan Zhao Hong-Wei Wang Xinquan Wang Zheng Zhang Linqi Zhang Qiang Zhou | 2021 | Cell Research2021，31，5： | 4 |
| 6 | Synthesis of MoX2 (X =Se or S) monolayers with high-concentration 1T'phase on 4H/fcc-Au nanorods for hydrogen evolution显示文摘Controlled synthesis of transition metal dichalcogenide (TMD) monolayers with unusual crystal phases has attracted increasing attention due to their promising applications in electrocatalysis.However,the facile and large-scale preparation of TMD monolayers with high-concentration unusual crystal phase still remains a challenge.Herein,we report the synthesis of MoX2 (X =Se or S) monolayers with high-concentration semimetallic 1T'phase by using the 4H/face-centered cubic (fcc)-Au nanorod as template to form the 4H/fcc-Au@MoX2 nanocomposite.The concentrations of 1T'phase in the prepared MoSe2 and MoS2 monolayers are up to 86% and 81%,respectively.As a proof-of-concept application,the obtained Au@MoS2 nanocomposite is used for the electrocatalytic hydrogen evolution reaction (HER) in acid medium,exhibiting excellent performance with a low overpotential of 178 mV at the current density of 10 mNcm^2,a small Tafel slope of 43.3 mV/dec,and excellent HER stability.This work paves a way for direct synthesis of TMD monolayers with high-concentration of unusual crystal phase for the electrocatalytic application. | Zhengqing Liu Xiao Zhang Yue Gong Qipeng Lu Zhicheng Zhang Hongfei Cheng Qinglang Ma Junze Chen Meiting Zhao Bo Chen Ye Chen Xue-Jun Wu Pengfei Yin Lin Gu Yaping Du Hua Zhang | 2019 | Nano Research2019，12，6： | 3 |
| 7 | Luminous composite ultrathin films of CdTe quantum dots/silk fibroin co-assembled with layered doubled hydroxide:Enhanced photoluminescence and biosensor application显示文摘Quantum dots(QDs)luminescent films are extensively applied to optoelectronics and optical devices.However,QDs aggregation results in the quenching of their fluorescence property which limits their practical applications to a greater extent.In order to resolve this issue,3-mercaptopropionic acid(3-MPA)functionalized Cadmium Tellurium(CdTe)QDs were stabilized by silk fibroin(SB)and coassembled with layered doubled hydroxide(LDH)to form(QDs@SF/LDH)_(n)ultrathin films(UTFs)via the layer-by-layer(LBL)technique.UV-Vis absorption and fluorescence spectroscopy showed a stepwise and normal growth of the films upon increasing the number of deposition cycles.XRD and AFM studies confirmed the formation of a periodic layered structure and regular surface morphology of the thin films.As compared to(CdTe QDs/LDH)_(n)UTFs,the(CdTe QDs@SF/LDH)_(n)UTFs displayed fluorescence enhancement and longer fluorescent lifetime,both in solid states and aqueous solutions.Furthermore compared with the solution state,the fluorescence enhancement of SF-RC and SF-β are,respectively,7 times and 17 times in the(CdTe QDs@SF/LDH)_(n)UTFs,indicating that the LDH nanosheets favor the fluorescence enhancement effect on the CdTe QDs@SF.The fabricated materials displayed fluorescence response to a biological molecule such as immune globulin,lgG.Thus,the(CdTe QDs@SF/LDH)_(n)UTFs has a potential to be used as biosensor. | Muhammad Sohail Haroone Ling Li Aftab Ahmad Yaping Huang Ruili Ma Ping Zhang Yuehua Hu Qari Muhammad Kaleem Jun Lu | 2018 | Journal of Materiomics2018，4，2： | 3 |
| 8 | Surface engineering of titania nanotubes incorporated with double-layered extracellular vesicles to modulate inflammation and osteogenesis显示文摘Titania nanotubes(TNT)generated on titanium implant are emerged as important modification technique to facilitate bone regeneration.Mesenchymal stem cells(MSCs)-derived exosomes are membrane bound extracellular vesicles(EVs),which play an important role in tissue regeneration.The objective of this study was to generate an EVs hybrid TNT aiming at regulating inflammation,MSCs recruitment and osteogenesis.We isolated EVs from MSCs(MSCs EVs)and 3-day osteogenically differentiated MSCs(3d EVs).MSC EVs and 3d EVs exhibited round morphology under TEM,which also showed robust internalization by human bone marrow derived MSCs(hBMSCs).Next,we fabricated 3d EVs/MSC EVs hybrid TNT.When inflammatory macrophages were co-cultured with EVs hybrid TNT,the gene and protein expression of inflammatory cytokine were significantly reduced.Macrophage morphology was also examined by confocal laser scanning microscopy(CLSM)and scanning electron microscopy(SEM).Further migratory ability study using hBMSCs indicated significant enhancement of MSCs migration in EVs hybrid TNT.In addition,we further demonstrated significant increase of osteogenic differentiation of hBMSCs in EVs hybrid TNT.This study suggests that EVs hybrid TNT may serve as a viable therapeutic approach to enhance osteogenesis and bone regeneration. | Qingyu Zhao Yi Zhang Lan Xiao Haiping Lu Yaping Ma Qi Liu Xin Wang | 2021 | Regenerative Biomaterials2021，8，3： | 3 |
| 9 | Genotypic variation in spatiotemporal distribution of canopy light interception in relation to yield formation in cotton显示文摘Background: Within-canopy interception of photosynthetically active radiation(PAR) impacts yield and other agronomic traits in cotton(Gossypium hirsutum L.). Field experiments were conducted to investigate the influence of 6 cotton varieties(they belong to 3 different plant types) on yield, yield distribution, light interception(LI), LI distribution and the relationship between yield formation and LI in Anyang, Henan, in 2014 and 2015.Result: The results showed that cotton cultivars with long branches(loose-type) intercepted more LI than did cultivars with short branches(compact-type), due to increased LI in the middle and upper canopy. Although loose-type varieties had greater LI, they did not yield significantly higher than compact-type varieties, due to decreased harvest index. Therefore, improving the harvest index by adjusting the source-to-sink relationship may further increase cotton yield for loose-type cotton. In addition, there was a positive relationship between reproductive organ biomass accumulation and canopy-accumulated LI, indicating that enhancing LI is important for yield improvement for each cultivar. Furthermore, yield distribution within the canopy was significantly linearly related to vertical LI distribution.Conclusion: Therefore, optimizing canopy structure of different plant type and subsequently optimizing LI distribution within the cotton canopy can effectively enhance the yield. | XING Fangfang HAN Yingchun FENG Lu ZHI Xiaoyu WANG Guoping YANG Beifang FAN Zhengyi LEI Yaping DU Wenli WANG Zhanbiao XIONG Shiwu LI Xiaofei Ll Yabing | 2018 | Journal of Cotton Research2018，1，3： | 3 |
| 10 | Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda显示文摘The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas. | Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang | 2022 | Protein & Cell2022，13，7： | 2 |
| 11 | Keratin 5-Cre-driven deletion of Ncstn in an acne inversa-like mouse model leads to a markedly increased IL-36a and Sprr2 expression显示文摘Familial acne inversa (AI) is an autoinflammatory disorder that affects hair follicles and is caused by loss-of-function mutations in y-secretase component genes.We and other researchers showed that nicastrin (NCSTN) is the most frequently mutated gene in familial AI.In this study,we generated a keratin 5-Cre-driven epidermis-specific Ncstn conditional knockout mutant in mice.We determined that this mutant recapitulated the major phenotypes of AI,including hyperkeratosis of hair follicles and inflammation.In Ncstnflox/flox;K5-Cre mice,the IL-36a expression level markedly increased starting from postnatal day 0 (P0),and this increase occurred much earlier than those of TNF-α,IL-23A,IL-1 3,and TLR4.RNA-Seq analysis indicated that Sprr2d,a member of the small proline-rich protein 2 family,in the skin tissues of the Ncstnflox/flox,;K5-Cre mice was also upregulated on P0.Quantitative reverse-transcription polymerase chain reaction showed that other Sprr2 genes had a similar expression pattern.Our findings suggested that IL-36a might be a key inflammatory cytokine in the pathophysiology of AI and implicate malfunction of the skin barrier in the pathogenesis of AI. | Jun Yang Lianqing Wang Yingzhi Huang Keqiang Liu Chaoxia Lu Nuo Si Rongrong Wang Yaping Liu Xue Zhang | 2020 | Frontiers of Medicine2020，14，3： | 2 |
| 12 | Dehydrated layered double hydroxides: Alcohothermal synthesis and oxygen evolution activity显示文摘分层的双氢氧化物(LDH ) 是一个班二维(2D ) 有在水的媒介的广泛的应用程序和开发得好的综合方法的分层的材料。在这个工作,我们为与使脱水的画廊制作 NiFe-LDHs 介绍一个 alcohothermal 合成方法。建议过程为金属离子的同时的降水包含脲的不完全的水解作用,与结果导致使脱水的结构的形成的水缺乏的乙醇环境。使画廊脱水的层结构的形成被 X 光检查衍射(XRD ) ,以及由一个随后的水和过程证实。这里介绍的方法论为制作基于 Fe 的 LDH (NiFe-LDH 和 NiCoFe-LDH ) 也是适用的 nanoarrays,它因为包含的不同降水过程,不能在水的媒介在一样的条件下面被生产。LDH nanoarrays 展览在氧进化反应的优秀 electrocatalytic 性能,由于他们的高内在的活动和唯一的结构的特征。在摘要,这研究不仅为综合介绍一个新方法 LDH 材料,而且为 electrocatalysis 向高度活跃、柔韧的电极提供一条新线路。 | Zhiyi Lu Li Qian Wenwen Xu Yang Tian Ming Jiang Yaping Li Xiaoming Sun Xue Duan | 2016 | Nano Research2016，9，10： | 2 |
| 13 | A new model for dust emission by saltation bombardment显示文摘 | Hua Lu Yaping Shao | 1999 | J Geophy Res1999，104，16： | 1 |
| 14 | The opposing effects of intracerebroventricularly injected norepinephrine on oxytocin and vasopressin neurons in the paraventricular nucleus of the rat显示文摘 | Mei Jun Lu Shaogang | 1998 | Neurosci Lett1998，244，1： | 1 |
| 15 | Multiple origins of Tibetan Y chromosomes显示文摘 | Yaping Qian Binzhi Qian Bing Su Jiankun Yu Yuehai Ke Zhengtao Chu Lei Shi Daru Lu Jiayou Chu Li Jin | 2000 | Human Genetics2000，，4： | 1 |
| 16 | Adenocarcinoma transformed into squamous cell carcinoma in non-small cell lung cancer显示文摘Non-small cell lung cancer(NSCLC)is the most common form of lung cancer which remains the deadliest malignancy worldwide(Siegel et al.,2019).In general,NSCLC can be divided into several subtypes,including adenocarcinoma(ADC),squamous cell carcinoma(SCC),adeno-squamous cell carcinoma(AD-SCC)and large cell carcinoma(LCC). | Yueqiang Jiang Jun Zhang Jin Feng Yaping Lu Yuan Fan Ling Cheng Xin Liao Liya Hu Shiying Yu Tiejun Yin | 2021 | Journal of Genetics and Genomics2021，48，7： | 1 |
| 17 | Toward quantitative prediction of dust storms:an integrated wind erosion modelling system and its applications 显示文摘 | Lu Hua Shao Yaping | 2001 | Environmental Modelling Software2001，16，3： | 1 |
| 18 | Study on the toxic effects induced by different arsenicals in primary cultured rat astroglia显示文摘 | Yaping Jin Guifan Sun Xin Li Gexin Li Chunwei Lu Long Qu | 2004 | Toxicology and Applied Pharmacology2004，，3： | 1 |
| 19 | Multiple origins of Tibetan Y chromosomes显示文摘 | Yaping Qian Binzhi Qian Bing Su Jiankun Yu Yuehai Ke Zhengtao Chu Lei Shi Daru Lu Jiayou Chu Li Jin | 2000 | Human Genetics2000，，4： | 1 |
| 20 | hClock gene expression in human colorectal carcinoma显示文摘 | Liying Wang Bozan Chen Yaping Wang Ning Sun Chao Lu Ruizhe Qian Luchun Hua | 2013 | Molecular Medicine Reports2013，，4： | 1 |
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