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| 1 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 2 | Matrix metalloproteinase-13 is regulated by toll-like receptor-9 incolorectal cancer cells and mediates cellular migration显示文摘 | Timo Rath Julia St?ckle Martin Roderfeld Annette Tschuschner Jürgen Graf Elke Roeb | 2011 | Oncology Letters2011，，3： | 2 |
| 3 | The psychological factors and effectiveness of postoperative lumbar disc hernia and lumbar spinal stenosis显示文摘 | Heno J Timo A Krohn G B | 2000 | Spine2000，32，1： | 1 |
| 4 | Single-cell analysis of reg- ulatory gene expression in quieseent and activated mouse skele- tal muscle satellite cells显示文摘 | COMELISON TIMO D WOLD J 13 | 2003 | Developmental Biology2003，191，： | 1 |
| 5 | Microbe repelling coated stainless steel analysed by field emission scanning electron microscopy and physicochemical methods显示文摘 | Mari Raulio Mikael J?rn Juhana Ahola Jouko Peltonen Jarl B. Rosenholm Sanna Tervakangas Jukka Kolehmainen Timo Ruokolainen Pekka Narko Mirja Salkinoja-Salonen | 2008 | Journal of Industrial Microbiology & Biotechnology2008，，7： | 1 |
| 6 | Clonal differences in growth and phenolics of willows exposed to elevated ultraviolet-B radiation显示文摘 | RIITTA T TIMO V PEDRO J A et ai | 2003 | Basic Appl Ecol2003，4，3： | 1 |
| 7 | TIMP-1/-2 and transient elastography allow non invasive diagnosis of cystic fibrosis associated liver disease显示文摘 | Timo Rath Katrin Menendez Menendez Marion Kügler Lisa Hage Christian Wenzel Richard Schulz Jürgen Graf Lutz N?hrlich Elke Roeb Martin Roderfeld | 2012 | Digestive and Liver Disease2012，，9： | 1 |
| 8 | The psychological factors and effectiveness of postoperative lumbar disc hernia and lumbar spinal stenosis显示文摘 | Heno J Timo A Krohngrimbenghe B | 2000 | Spine2000，25，1： | 1 |
| 9 | 5-HT3 Receptor antagonists in the prophylaxis of acute vomiting induced by moderately emetogenic chemotherapy a randomised study显示文摘 | Timo J Vesa V | 1993 | Eur J Cancer1993，29，12： | 1 |
| 10 | lnvestigating Stability and Linearity of a German M 1 Money Demand Function显示文摘 | Helmut L ti tkepohl Timo Teaavirta J u rgen Wolters | 1999 | Jourhal of Applied Econometrics1999，，14： | 1 |
| 11 | The bacterial ghost platform system production and applications 显示文摘 | Timo L Verena J K Abbas M | 2010 | Bioengineered Bugs2010，1，5： | 1 |
| 12 | 显示文摘 | Timo thy S Haddad R S Hong G J | 1999 | Polymer Preprints1999，40，1： | 1 |
| 13 | Effect of 12‐month statin therapy on antioxidant potential of LDL and serum antioxidant vitamin concentrations显示文摘 | Tommi Vasankari Markku Ahotupa Jorma Viikari Ilpo Nuotio Timo Strandberg Hannu Vanhanen Helena Gylling Tatu Miettinen Matti J Tikkanen | 2004 | Annals of Medicine2004，，： | 1 |
| 14 | Valproic acid combined with 13-cis retinoic acid and 1,25-dihydroxyvitamin D3 in the treatment of patients with myelodysplastic syndromes 显示文摘 | Timo S Timo T Eeva J | | Haematologica0，92，8： | 1 |
| 15 | Cardiovascular autonomic function correlates with the response to aerobic training in healthy sedentary subjects 显示文摘 | Hautala Arto J Timo H | 2003 | Am J Physiol Heart Circ Physiol2003，285，： | 1 |
| 16 | The Psychological Factors and Effectiveness of Postoperative Lumbar Disc Hernia and Lumbar Spinal Stenosis显示文摘 | Heno J Timo A Krohn-Grimbenghe B | 2000 | Spine2000，32，1： | 1 |
| 17 | Navigated versus conventional transfixation of AC joint injuries: Feasibility and accuracy显示文摘 | Timo Stü big Torsten J? hnisch Maximilian Petri Nael Hawi Christian Zeckey Christian Krettek Musa Citak Rupert Meller | 2013 | Computer Aided Surgery . 2013 (3-4)2013，，： | 1 |
| 18 | Evaluating cataract surgery gains by assessing patients’ quality of life using the VF-7 1 1 Mari Piik, Kaija Aalto, and Helena Ivaska conducted the patient interviews.显示文摘 | Risto J Uusitalo Tiina Brans Timo Pessi Ahti Tarkkanen | 1999 | Journal of Cataract & Refractive Surgery1999，，7： | 1 |
| 19 | Twist predicts poor outcome of patients with astrocytic glioma显示文摘 | Nordfors Kristiina Haapasalo Joonas Makela Katri Granberg Kirsi J Nykter Matti Korja Miikka Paavonen Timo Haapasalo Hannu Soini Ylermi | 2015 | Journal of Clinical Pathology2015，，11： | 1 |
| 20 | The psychological factors and effectiveness of postoperative lumbar disc hernia and lumbar spinal stenosis显示文摘 | Heno J Timo A Krohn GB | 2000 | Spine2000，32，1： | 1 |
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