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| 1 | Enhanced migration of tissue inhibitor of metalloproteinase overexpressing hepatoma cells is attributed to gelatinases: Relevance to intracellular signaling pathways显示文摘AIM: To study the effect of gelatinases (especially MMP-9)on migration of tissue inhibitor of metalloproteinase (TIMP-1) overexpressing hepatoma cells.METHODS: Wild type HepG2 cells, cells stably transfected with TIMP-1 and TIMP-1 antagonist (MMP-9-H401A, a catalytically inactive matrix metalloproteinase (MMP) which still binds and neutralizes TIMP-1) were incubated in Boyden chambers either with or without Galardin (a synthetic inhibitor of MMP-1, -2, -3, -8, -9) or a specific inhibitor of gelatinases.RESULTS: Compared to wild type HepG2 cells, the cells overexpressing TIMP-1 showed 115% migration (P<0.05)and the cells overexpressing MMP-9-H401A showed 62% migration (P<0.01). Galardin reduced cell migration dose dependently in all cases. The gelatinase inhibitor reduced migration in TIMP-1 overexpressing cells predominantly.Furthermore, we examined intracellular signal transduction pathways of TIMP-1-dependent HepG2 cells. TIMP-1deactivates cell signaling pathways of MMP-2 and MMP-9involving p38 mitogen-activated protein kinase. Specific blockade of the ERK pathway suppresses gelatinase expression either in the presence or absence of TIMP-1.CONCLUSION: Overexpressing functional TIMP-1-enhanced migration of HepG2-TIMP-1 cells depends on enhanced MMP-activity, especially MMP-9. | Elke Roeb Anja-Katrin Bosserhoff Sabine Hamacher Bettina Jansen Judith Dahmen Sandra Wagner Siegfried Matern | 2005 | World Journal of Gastroenterology2005，11，8： | 7 |
| 2 | Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC显示文摘AIM:To investigate the proteolytic contribution of tumor-associated macrophages(TAM)in tumor invasion,we analyzed whether TAM at the invasive front of small HCC in Abcb4-/--mice show an enhanced expression of MMP-9. METHODS:Liver cryosections of the hepatocellular carcinoma(HCC)invasive front from 12 mo old Abcb4-/--mice were stained for collagen typeⅠand MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies.Afterwards,the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies.Finally, photographs of the invasive tumor front were digitally overlaid and analyzed. RESULTS:After complete bleaching of the primary dye,specific fluorescence staining of a third antigen, here F4/80,was successfully performed on the same histological section.With this method,we were able to identify conglomerates of matrix metalloproteinase (MMP-9)expressing macrophages within the tumor capsule of HCC. CONCLUSION:MMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC.The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry,facilitating visualization of at least three different antigens plus nuclei in one single histological section. | Martin Roderfeld Timo Rath Frank Lammert Christian Dierkes Jürgen Graf Elke Roeb | 2010 | World Journal of Hepatology2010，2，5： | 6 |
| 3 | Matrix metalloproteinase-13 is regulated by toll-like receptor-9 incolorectal cancer cells and mediates cellular migration显示文摘 | Timo Rath Julia St?ckle Martin Roderfeld Annette Tschuschner Jürgen Graf Elke Roeb | 2011 | Oncology Letters2011，，3： | 2 |
| 4 | Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane 显示文摘 | Sohlenkamp C Wood C C Roeb G W | 2002 | Plant Physiology2002，130，4： | 1 |
| 5 | Matrixmetalloproteinases and colorectal cancer显示文摘 | Roeb E Matern S | 2003 | Med Klin2003，98，12： | 1 |
| 6 | Activity and cellular origin of gelatinases in patients with colon and rectal carcinoma显示文摘 | Roeb E Dietrich CG Winograd R | 2010 | Cancer2010，92，： | 1 |
| 7 | TIMP-1/-2 and transient elastography allow non invasive diagnosis of cystic fibrosis associated liver disease显示文摘 | Timo Rath Katrin Menendez Menendez Marion Kügler Lisa Hage Christian Wenzel Richard Schulz Jürgen Graf Lutz N?hrlich Elke Roeb Martin Roderfeld | 2012 | Digestive and Liver Disease2012，，9： | 1 |
| 8 | Economic comparison of solar hydrogen generation by means of thermochemical cycles and electrolysis显示文摘 | D. Graf N. Monnerie M. Roeb M. Schmitz C. Sattler | 2008 | International Journal of Hydrogen Energy2008，，17： | 1 |
| 9 | Activity and cellular origin of gelatinases in patients with colon and rectal carcinoma differential activity of matrix metalloproteinase-9 显示文摘 | Roeb E Dietrich CG Winograd R | 2001 | Cancer2001，92，10： | 1 |
| 10 | Solar Hydrogen Production by a Two-Step Cycle Based on Mixed Iron Oxides 显示文摘 | Roeb M Christian S Kliiser R | 2006 | Journal of Solar Energy Engineering2006，，128： | 1 |
| 11 | Extrace llularmatrix -from basic research to clinical significance:an overview with special concideration of matrix metallop roteinases显示文摘 | Hamacher S Matem S Roeb E | 2004 | Dtsch Med Wochenschr2004，129，38： | 1 |
| 12 | Matrix matelloproteinases and colorectal cancer显示文摘 | Roeb E Matern S | 2003 | Med Klin(Munich)2003，98，12： | 1 |
| 13 | Concentrating on Solar Electricity and Fuels 显示文摘 | M Roeb H Miiler-Steinhagen | 2010 | Science2010，329，5993： | 1 |
| 14 | Mechanisms of fibrinolysis in chronic liver injury (with special emphasis on MMPs and TIMPs) 显示文摘 | Roderfeld M Hemmann S Roeb E | 2007 | Z Gastroenterol2007，45，1： | 1 |
| 15 | PAX3-FOXO1 controls expression of the p57Kip2 cell-cycle regulator through degradation of EGR1 显示文摘 | Roeb W Boyer A Cavenee WK | 2007 | PNAS2007，104，18： | 1 |
| 16 | Matrix metalloproteinase and colorectal cancers显示文摘 | Roeb E Matem S | 2003 | Med Klim(Munich)2003，98，12： | 1 |
| 17 | Extracellular matrix-from basic research to clinical significance:an overview with special consideration of matrix metalloproteinases 显示文摘 | Hamacher S Matern S Roeb E | 2004 | Dtsch Med Wochenschr2004，129，38： | 1 |
| 18 | TIMP expression in toxic and cholestatic liver injury in rat显示文摘 | Purucker E Breuer B | 1997 | J Hepatol1997，27，3： | 1 |
| 19 | Mechanisms of fibrinolysis in chronic liver injury (with special emphasis on MMPs and TIMPs) 显示文摘 | Roderfeld M Hemmann S Roeb E | 2007 | Z Gastroen- terol2007，45，1： | 1 |
| 20 | Accumulation of assimilates in stem-rust-infected wheat leaves显示文摘 | Keutgen N Roeb G W Fuhr F | 1995 | Journal of Agronomy and Crop Science1995，175，5： | 1 |
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