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| 1 | Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)显示文摘The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens.Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence.Messenger RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic lines.Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance.The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate.Data recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more yield.Moreover,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size. | Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN Tayyab HUSNAIN | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，4： | 4 |
| 2 | In vitro Selection for Fusarium Wilt Resistance in Gladiolus显示文摘Cormels pieces of four Fusarium susceptible Gladiolus cultivars (Friendship,Peter Pears,Victor Borge and Novalux) formed friable calli when cultured in vitro on Murashige and Skoog basal medium containing various concentrations of auxin and cytokinin. The friable calli established cell suspensions. Plantlet regeneration was obtained from the control callus,control cell suspension derived callus and in vitro selected Fusarium oxysporum Schlecht. resistant cell-lines of Friendship. The in vitro cormlets showed 85–95% germination after breaking dormancy of 8weeks at 4 ℃. Cell suspensions of all four Gladiolus cultivars were found to be highly sensitive to fusaric acid. Gradual increase in fusaric acid concentrations to the cell-suspension cultures decreased cell growth considerably. One albino plant was found from the second generation of the in vitro selected cell line of Friendship. The albino plant was found to be highly susceptible to F. oxysporum. The cormlets of all in vitro selected cell lines of Friendship were inoculated with a conidial suspension of the F. oxysporum before planting and were also sprayed with the same spore suspension for further characterization when the height of plants was about 6 cm. The four selected cell lines showed the same response whether or not they were inoculated with conidia of the F. oxysporum. Plantlets of all of the selected cell lines exhibited significant growth as compared with the control after application of conidia of the F. oxysporum. | Idrees Ahmad Nasir Sheikh Riazuddin | 2008 | Journal of Integrative Plant Biology2008，50，5： | 2 |
| 3 | Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds显示文摘Background:Following recent findings from our group that curcumin preconditioning augments the therapeutic efficacy of adipose-derived stem cells in the healing of diabetic wounds in rats,we aimed to investigate the regenerative effects of curcumin preconditioned adipose-derived mesenchymal stem cells(ASCs)for better recovery of acid inflicted burns in this study.Methods:ASCs were preconditioned with 5μM curcumin for 24 hours and assessed for proliferation,migration,paracrine release potential and gene expression comparative to naive ASCs.Subsequently,the healing capacity of curcumin preconditioned ASCs(Cur-ASCs)versus naive ASCs was examined using acidic wounds in rats.For this,acid inflicted burns of 20 mm in diameter were made on the back of male Wistar rats.Then,2×10^(6) cells of Cur-ASCs and naive ASCs were intradermally injected in the wound periphery(n=6)for comparison with an untreated saline control.Post-transplantation,wounds were macroscopically analysed and photographed to evaluate the percentage of wound closure and period of re-epithelization.Healed wound biopsies were excised and used for histological evaluation and expression analysis of wound healing markers at molecular level by quantitative PCR and western blotting.Results:We found that Cur-ASCs exhibited greater proliferation,migration and paracrine potential in vitro.Further,Cur-ASCs showed more effective recovery than naive ASCs as exhibited by gross morphology,faster wound closure and earlier re-epithelialization.Masson’s trichrome and hematoxylin and eosin staining demonstrated the improved architecture of the healing burns,as evidenced by reduced infiltration of inflammatory cells,compact collagen and marked granulation in Cur-ASC treated rats.Corroborating these findings,molecular assessment showed significantly reduced expressions of pro-inflammatory factors(interleukin-1 beta,interleukin-6,tumor necrosis factor alpha)a with striking upsurge of an oxidative marker(superoxide dismutase 1),proangiogenic factors(vascular endothelial growth factor,hepatocyte growth factor,hypoxia-inducible factor-1 alpha)and collagen markers(transforming growth factor beta 1,fibroblast growth factor-2,collagen type 1 alpha 1),verifying that Cur-ASCs modulate the regulation of pro-inflammatory and healing markers at burn sites.Conclusions:Treatment with Cur-ASCs resulted in faster re-epithelization of acid inflicted burns compared to the treatment with naive ASCs.Based on observed findings,we suggest the transplantation of Cur-ASCs is a valuable therapy for the potent clinical management of acidic burns. | Maryam Azam Hafiz Ghufran Hira Butt Azra Mehmood Ramla Ashfaq Asad M.Ilyas Muhammad R.Ahmad Sheikh Riazuddin | 2021 | Burns & Trauma2021，9，1： | 2 |
| 4 | Bacillus thuringiensis isolates active against sucking insects 显示文摘 | RIAZUDDIN SHEIKH | | United States Patent:60，156，： | 1 |
| 5 | Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests显示文摘 | Shahina Bano Maqbool Sheikh Riazuddin Nguyen Thi Loc Angharad M.R. Gatehouse John A. Gatehouse Paul Christou | 2001 | Molecular Breeding2001，，1： | 1 |
| 6 | Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests显示文摘 | Shahina Bano Maqbool Sheikh Riazuddin Nguyen Thi Loc Angharad M.R. Gatehouse John A. Gatehouse Paul Christou | 2001 | Molecular Breeding2001，，1： | 1 |
| 7 | Enhanced hepatic differentiation of mesenchymal stem cells after pretreatment with injured liver tissue显示文摘 | Sadia Mohsin Sulaiman Shams Ghazanfar Ali Nasir Mohsin Khan Sana Javaid Awan Shaheen N. Khan Sheikh Riazuddin | 2010 | Differentiation2010，，1： | 1 |
| 8 | Functional Null Mutations of MSRB3 Encoding Methionine Sulfoxide Reductase Are Associated with Human Deafness DFNB74显示文摘 | Zubair M. Ahmed Rizwan Yousaf Byung Cheon Lee Shaheen N. Khan Sue Lee Kwanghyuk Lee Tayyab Husnain Atteeq Ur Rehman Sarah Bonneux Muhammad Ansar Wasim Ahmad Suzanne M. Leal Vadim N. Gladyshev Inna A. Belyantseva Guy Van Camp Sheikh Riazuddin Thomas B. Fri | 2011 | The American Journal of Human Genetics2011，，1： | 1 |
| 9 | RNA interference: The story of gene silencing in plants and humans显示文摘 | Mahmood-ur-Rahman Imran Ali Tayyab Husnain Sheikh Riazuddin | 2008 | Biotechnology Advances2008，，3： | 1 |
| 10 | Expression of multiple insecticidal sines confers broad resistance against a range of different rice pests显示文摘 | Shahina Bano Maqbool Sheikh Riazuddin Nguyen Thi Loc et ai | 2001 | Molecular Breeding2001，7，1： | 1 |
| 11 | Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience显示文摘AIM: To assess the association between chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) in Pakistan, and the genotype distribution among these HCC patients.METHODS: One hundred and sixty-one subjects with HCC were included in this study. Liver biopsy was performed on 145 of the patients; sixteen were excluded because they failed to fulfill the inclusion criteria. Qualitative polymerase chain reaction (PCR) was performed for hepatitis B virus and HCV. Samples positive for HCV RNA were genotyped using genotype-specif ic PCR and conf irmed by HCV 5' noncoding region sequencing analysis. RESULTS: Chronic HCV infection was identified a major risk factor (63.44% of tested HCC patients) forthe development of HCC. The time from HCV infection to appearance of cancer was 10-50 years. In the HCC patient population, broader distributions of genotypes were present with genotype 3a as the predominant genotype. Using the type-specific genotyping method, we found HCV genotype 3a in 40.96%, 3b in 15.66%, 1a in 9.63%, and 1b in 2.40% of HCC tissue samples. About 28% of cases were found with mixed genotypes. Two cases were unable to be genotyped because of low viral load. Sixty-six percent of treated patients with cirrhosis had an end of treatment response, but unfortunately they relapsed quickly when the treatment was discontin-ued, and HCC developed during a median 3.8 years. CONCLUSION: There was a strong association between chronic HCV infection and HCC in Pakistan, and between HCV genotype 3a and HCC. | Muhammad Idrees Shazia Rafi que Irshad-ur Rehman Haji Akbar Muhammad Zubair Yousaf Sadia Butt Zunaira Awan Sobia Manzoor Madiha Akram Mahwish Aftab Bushra Khubaib Sheikh Riazuddin | 2009 | World Journal of Gastroenterology2009，15，40： | 1 |
| 12 | RNAi as a new therapeutic strategy against HCV显示文摘 | Saba Khaliq Sadaf A. Khaliq Muzna Zahur Bushra Ijaz Shah Jahan Muhammad Ansar Sheikh Riazuddin Sajida Hassan | 2009 | Biotechnology Advances2009，，1： | 1 |
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