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| 1 | Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)显示文摘Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods. | RAO Abdul Qayyum HUSSAIN S. Sarfraz SHAHZAD M. Saqib BOKHARI S. Yassir Abbas RAZA M. Hashim RAKHA Allah MAJEED A. SHAHID A. Ali SALEEM Zafar HUSNAIN Tayyab RIAZUDDIN S. | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，4： | 9 |
| 2 | Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)显示文摘The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens.Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence.Messenger RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic lines.Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance.The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate.Data recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more yield.Moreover,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size. | Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN Tayyab HUSNAIN | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，4： | 4 |
| 3 | In vitro Selection for Fusarium Wilt Resistance in Gladiolus显示文摘Cormels pieces of four Fusarium susceptible Gladiolus cultivars (Friendship,Peter Pears,Victor Borge and Novalux) formed friable calli when cultured in vitro on Murashige and Skoog basal medium containing various concentrations of auxin and cytokinin. The friable calli established cell suspensions. Plantlet regeneration was obtained from the control callus,control cell suspension derived callus and in vitro selected Fusarium oxysporum Schlecht. resistant cell-lines of Friendship. The in vitro cormlets showed 85–95% germination after breaking dormancy of 8weeks at 4 ℃. Cell suspensions of all four Gladiolus cultivars were found to be highly sensitive to fusaric acid. Gradual increase in fusaric acid concentrations to the cell-suspension cultures decreased cell growth considerably. One albino plant was found from the second generation of the in vitro selected cell line of Friendship. The albino plant was found to be highly susceptible to F. oxysporum. The cormlets of all in vitro selected cell lines of Friendship were inoculated with a conidial suspension of the F. oxysporum before planting and were also sprayed with the same spore suspension for further characterization when the height of plants was about 6 cm. The four selected cell lines showed the same response whether or not they were inoculated with conidia of the F. oxysporum. Plantlets of all of the selected cell lines exhibited significant growth as compared with the control after application of conidia of the F. oxysporum. | Idrees Ahmad Nasir Sheikh Riazuddin | 2008 | Journal of Integrative Plant Biology2008，50，5： | 2 |
| 4 | Curcumin preconditioning enhances the efficacy of adipose-derived mesenchymal stem cells to accelerate healing of burn wounds显示文摘Background:Following recent findings from our group that curcumin preconditioning augments the therapeutic efficacy of adipose-derived stem cells in the healing of diabetic wounds in rats,we aimed to investigate the regenerative effects of curcumin preconditioned adipose-derived mesenchymal stem cells(ASCs)for better recovery of acid inflicted burns in this study.Methods:ASCs were preconditioned with 5μM curcumin for 24 hours and assessed for proliferation,migration,paracrine release potential and gene expression comparative to naive ASCs.Subsequently,the healing capacity of curcumin preconditioned ASCs(Cur-ASCs)versus naive ASCs was examined using acidic wounds in rats.For this,acid inflicted burns of 20 mm in diameter were made on the back of male Wistar rats.Then,2×10^(6) cells of Cur-ASCs and naive ASCs were intradermally injected in the wound periphery(n=6)for comparison with an untreated saline control.Post-transplantation,wounds were macroscopically analysed and photographed to evaluate the percentage of wound closure and period of re-epithelization.Healed wound biopsies were excised and used for histological evaluation and expression analysis of wound healing markers at molecular level by quantitative PCR and western blotting.Results:We found that Cur-ASCs exhibited greater proliferation,migration and paracrine potential in vitro.Further,Cur-ASCs showed more effective recovery than naive ASCs as exhibited by gross morphology,faster wound closure and earlier re-epithelialization.Masson’s trichrome and hematoxylin and eosin staining demonstrated the improved architecture of the healing burns,as evidenced by reduced infiltration of inflammatory cells,compact collagen and marked granulation in Cur-ASC treated rats.Corroborating these findings,molecular assessment showed significantly reduced expressions of pro-inflammatory factors(interleukin-1 beta,interleukin-6,tumor necrosis factor alpha)a with striking upsurge of an oxidative marker(superoxide dismutase 1),proangiogenic factors(vascular endothelial growth factor,hepatocyte growth factor,hypoxia-inducible factor-1 alpha)and collagen markers(transforming growth factor beta 1,fibroblast growth factor-2,collagen type 1 alpha 1),verifying that Cur-ASCs modulate the regulation of pro-inflammatory and healing markers at burn sites.Conclusions:Treatment with Cur-ASCs resulted in faster re-epithelization of acid inflicted burns compared to the treatment with naive ASCs.Based on observed findings,we suggest the transplantation of Cur-ASCs is a valuable therapy for the potent clinical management of acidic burns. | Maryam Azam Hafiz Ghufran Hira Butt Azra Mehmood Ramla Ashfaq Asad M.Ilyas Muhammad R.Ahmad Sheikh Riazuddin | 2021 | Burns & Trauma2021，9，1： | 2 |
| 5 | Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests显示文摘 | Maqbool S B Riazuddin S Loc N T | 2001 | Mol Breed2001，7，： | 1 |
| 6 | Alterations of the CIB2calcium-and integrin-binding protein cause Usher syndrome type 1J and nonsyndromic deafness DFNB48显示文摘 | Riazuddin S Belyantseva IA Giese AP | 2012 | Nat Genet2012，44，11： | 1 |
| 7 | Novel mutations of MYO15A associated with profound deafness in consanguineous families and moderately severe hearing loss in a patient with Smith-Magenis syndrome 显示文摘 | Liburd N Ghosh M Riazuddin S | 2001 | Hum Genet2001，109，： | 1 |
| 8 | Expression of multiple insecticidal genes confers bread resistance against a range of different rice pests显示文摘 | Maqbool S B Riazuddin S Loc N T | 2001 | Molecular Breeding2001，，7： | 1 |
| 9 | Ascochyta blight of chick- pea production of phytotoxins and disease management显示文摘 | Shahid A A Husnain T Riazuddin S | 2008 | Bio- technol Adv2008，26，6： | 1 |
| 10 | Mutations ofMY06 are associated with recessive deafness, DFNB37显示文摘 | Ahmed ZM Morell RJ Riazuddin S | 2003 | Am J Hum Genet2003，72，5： | 1 |
| 11 | Mutations of MYO6 are associated with recessive deafness, DFNB37显示文摘 | Ahmed ZM Morell RJ Riazuddin S | 2003 | Am J Hum Genet2003，72，5： | 1 |
| 12 | Usher syndrome 1D and non--syndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of the novel cadherin-- like gene CDH23显示文摘 | Bork JM Peters LM Riazuddin S | 2001 | Am J Human Genetics2001，68，： | 1 |
| 13 | Bacillus thuringiensis isolates active against sucking insects 显示文摘 | RIAZUDDIN SHEIKH | | United States Patent:60，156，： | 1 |
| 14 | Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests显示文摘 | Shahina Bano Maqbool Sheikh Riazuddin Nguyen Thi Loc Angharad M.R. Gatehouse John A. Gatehouse Paul Christou | 2001 | Molecular Breeding2001，，1： | 1 |
| 15 | Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)显示文摘 | RAO Abdul Qayyum HUSSAIN S. Sarfraz SHAHZAD M. Saqib BOKHARI S. Yassir Abbas RAZA M. Hashim RAKHA Allah MAJEED A. SHAHID A. Ali SALEEM Zafar HUSNAIN Tayyab RIAZUDDIN S | | Journal of Zhejiang University Science0，，： | 1 |
| 16 | Distinctive Audiometric Profile Associated with DFNB21 Alleles of TECTA显示文摘 | Naz S Alasti F Mowjoodi A Riazuddin S Sanati MH | | 0，，： | 1 |
| 17 | Mutations in a novel gene,TMIE,are associated with hearing loss linked to the DFNB6 locus显示文摘 | Naz S Giguere CM Kohrman DC Mitchem KL Riazuddin S Morell RJ Ramesh A Srisailpathy S Deshmukh D Riazuddin S Griffith AJ Friedman TB Smith RJ Wilcox ER | | 0，，03： | 1 |
| 18 | Mutations of the protocadherin gene PCDH15 cause Usher syndrome type 1F显示文摘 | Ahmed Z M Riazuddin S Bernstein S L | 2001 | Am J Hum Genet2001，69，1： | 1 |
| 19 | Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)显示文摘 | Abdul Qayyum Rao S. Sarfraz Hussain M. Saqib Shahzad S. Yassir Abbas Bokhari M. Hashim Raza Allah Rakha A. Majeed A. Ali Shahid Zafar Saleem Tayyab Husnain S. Riazuddin | | 0，，： | 1 |
| 20 | Mutations of ESPN cause autosomal recessive deafness and vestibular dysfunc-tion显示文摘 | Naz S Griffith AJ Riazuddin S Hampton LL Battey JF Jr Khan SN Riazuddin S Wilcox ER Friedman TB | | 0，，08： | 1 |
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