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| 1 | 河北小五台山国家级自然保护区森林群落与环境的关系显示文摘植物与环境之间的关系是一个复杂的演变过程,运用数量生态学方法探讨森林群落的物种组成、种群的生态特征、不同植物群落与环境之间的关系,有助于保护该区森林群落的稳定性和生物多样性。根据148个森林群落样方数据,选用双向指示种分析(TWINSPAN)和典范对应分析(CCA)方法,对河北小五台山国家级自然保护区森林群落进行分类和排序研究。结果表明:(1)TWINSPAN将该区的森林群落分为20个类型;(2)CCA排序结果较好地反映出群落分布格局与环境梯度的关系,各个森林群落类型在前两轴分异明显,在11个环境因子中,海拔、坡位、凋落层厚度、土壤导电率、土壤温度、土壤湿度、土壤厚度和干扰程度这8个环境因子对森林群落的分布起较大的作用,影响森林群落的分布格局,形成不同的植被类型。(3)乔木层优势种的CCA二维排序图所揭示的环境梯度与群落类型的分布有很大的相似性;t值双序图阐明了海拔、凋落层厚度、土壤温度、干扰程度等环境因子对森林群落乔木层优势种有着重要影响。采用TWINSPAN分类与CCA排序的方法,较好地解释了森林群落与环境因子的关系,为小五台山地区森林生态系统的科学管理和保护提供了理论依据,研究结果也为同类地区森林生态系统研究及保护提供参考和借鉴。 | 白晓航 张金屯 曹科 王云泉 Sehrish Sadia 曹格 | 2017 | 生态学报2017，37，11： | 32 |
| 2 | 黑参多糖抗疲劳作用的分子机制显示文摘目的:探讨黑参多糖对疲劳型小鼠机体的改善作用及其抗疲劳作用机制。方法:通过对小鼠进行负重游泳实验,建立一种由肌肉锻炼引起的疲劳模型,小鼠灌胃黑参多糖(50、100、200 mg/(kg mb·d))28 d后,记录力竭游泳时间,测定血清中血乳酸(blood lactic acid,BLA)、血清尿素氮(blood urea nitrogen,BUN)、白细胞介素(interleukin,IL)-6水平,检测肝脏中肝糖原、丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,应用免疫印迹实验评估促炎因子IL-1β、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达水平,通过逆转录实时荧光定量聚合酶链式反应法分析小鼠骨骼肌中过氧化物酶体增殖物激活受体γ共激活因子1α(peroxisome proliferator-activated receptorγcoactivator-1α,PGC-1α)、过氧化物酶体增殖物激活受体α(atherosclerosisperoxisomeproliferator activated receptoryα,PPARα)基因表达情况。结果:与负重游泳对照组相比,黑参多糖显著延长负重游泳组小鼠负重游泳时间(P<0.05);与不负重游泳对照组相比,黑参多糖显著降低血清中BLA、BUN、IL-6和肝脏中MDA水平(P<0.05),提高肝脏中SOD、GSH-Px活力和肝糖原含量(P<0.05),抑制肝脏中与炎症反应相关的IL-1β和TNF-α表达,同时显著提高PGC-1α、PPARα的表达水平(P<0.05)。结论:黑参多糖可以通过调节骨骼肌中PGC-1α、PPARα基因表达和抑制肝部炎症反应发挥抗疲劳作用。 | 刘兴龙 赵迎春 陈雪艳 佘欣鑫 SADIA Khatoon 姜颖 解慧勇 蒋白楠 郑毅男 刘文丛 丁传波 | 2020 | 食品科学2020，41，5： | 21 |
| 3 | 小五台山亚高山草甸的群落特征及物种多样性显示文摘采用样带和样方相结合的方法沿海拔梯度取样,利用数量生态学方法对小五台山国家级自然保护区亚高山草甸群落进行分析。结果表明,1)双向指示种分析(TWINSPAN)将该区的草甸群落分为9个类型;2)RDA排序结果较好地反映出群落分布格局与环境梯度的关系,各个群落类型在前两轴分异明显;第一轴反映了群落分布格局与坡向、土壤温度、海拔、土壤厚度极显著相关(P<0.01),与土壤湿度显著相关(P<0.05);第二轴反映了群落分布格局与坡度、土壤厚度极显著相关(P<0.01);3)环境因子的蒙特卡罗随机置换检验表明,坡向、海拔、坡度显著影响群落分布(P<0.05),对植被类型的形成具有关键作用;4)草甸群落Patrick丰富度指数随着海拔的升高呈下降趋势,随着坡向值的增加、土壤温度的升高呈逐渐上升趋势;Shannon-Wiener多样性指数随土壤厚度的增大呈微小下降趋势;Pielou均匀度指数随着海拔的升高、土壤湿度的增大呈上升趋势,随着坡向值的增加、土壤温度的升高呈逐渐下降趋势;Simpson优势度指数随着坡度的增大表现出微小的下降趋势,随土壤厚度的增大呈上升趋势。 | 白晓航 张金屯 曹科 王云泉 Sehrish Sadia 曹格 | 2016 | 草业科学2016，33，12： | 17 |
| 4 | Molecular cloning and expression profile of an abiotic stress and hormone responsive MYB transcription factor gene from Panax ginseng显示文摘v-myb 鸟的 myeloblastosis 病毒的 oncogene 相当或相同的事物 MYB 家庭组成抄写因素的最丰富的组之一并且在植物在发展过程和防卫回答起重要作用。人参人参属人参 C ·迈耶·米布基因被克隆并且指定了为 PgMYB1。PgMYB1 的 cDNA 长是 762 基础对并且编码在于的 R2R3 类型蛋白质 238 氨基酸。Subcellular 本地化证明那 PgMYB1-mGFP5 熔化蛋白质在原子核是明确地局部性的。理解 PgMYB1 的功能的角色,我们在不同纸巾并且在各种各样的条件下面调查了 PgMYB1 的表示模式。量的即时聚合酶链反应和西方的污点分析证明 PgMYB1 比在茎和种子在根,叶子,和侧面的根在高水平被表示。PgMYB1 的表示由 abscisic 酸,水杨酸酸, NaCl,和寒冷是起来调整的由甲基寒心、下面调整的 jasmonate。这些结果建议 PgMYB1 可能涉及对环境压力和荷尔蒙作出回应。 | Sadia Afrin Jie Zhu Hongzhe Cao Jingjia Huang Hao Xiu Tiao Luo Zhiyong Luo | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 15 |
| 5 | 不同沙棘籽提取物的抗氧化和抗菌活性研究显示文摘使用Soxhlet提取装置,用氯仿、乙酸乙酯、丙酮、甲醇4种不同溶剂对沙棘籽分别进行8小时萃取。粗提物被用来进行抗氧化和抗菌活性筛选。在离体模型(清除DPPH自由基和脂质体模型系统)中的粉末减少和抗氧化活性评价显示在各种提取物中使用甲醇作为萃取剂的提取物具有最高的抗氧化活性。甲醇提取物也具有最高的抗菌活性。最低抑制浓度(MIC)评价显示,在加入甲醇提取物对蜡状芽孢杆菌、凝结芽孢杆菌、枯草芽孢杆菌、单核细胞增生利斯特菌、小肠结肠炎耶尔森氏菌进行培养,MIC浓度分别为200,300,300,300,和350ppm。研究结果表明,沙棘种子应用于医药和食品储藏。 | P. S. Negi A. S. Chauhan G. A. Sadia Y. S. Rohinishree R. S. Ramteke 郭海 郭敏 | 2006 | 国际沙棘研究与开发2006，4，1： | 11 |
| 6 | JA-mediated transcriptional regulation of secondary metabolism in medicinal plants显示文摘植物生产在植物环境相互作用并且对关於生命、不能生活的压力起关键作用的大量第二等的代谢物。而且,许多第二等的代谢物为大量疾病有药品的功效(癌症,疟疾,等等) 。biosynthetic 基因的控制抄写是在植物调整第二等的新陈代谢的主要机制之一。象 MYC, MYB, WRKY 和 AP2/ERF 那样的几个抄写因素家庭被发现了在不同药用的植物涉及第二等的新陈代谢的规定。另外,生合成和第二等的代谢物的合适的累积被表明分子 jasmonic 酸(JA ) 也导致。这评论提供卓见进在药用的植物种类的一个范围表明小径和第二等的新陈代谢(vinblastine,烟碱, artemisinin, taxol 和人参皂甙) 的调停 JA 的 transcriptional 规定的 JA。 | Sadia Afrin Jing-Jia Huang Zhi-Yong Luo | 2015 | Science Bulletin2015，60，12： | 10 |
| 7 | Germacrone Induces Apoptosis in Human Hepatoma HepG2 Cells through Inhibition of the JAK2/STAT3 Signalling Pathway显示文摘Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-induced apoptosis in HepG2 cells. HepG2 cells were incubated with germacrone for 24 h, the protein expression of p-STAT3, STAT3, p-JAK2 and JAK2 was detected by Western Blotting, and RT-PCR was used to determine the expression of STAT3, p53, Bcl-2 and Bax at transcriptional levels. Besides that, HepG2 cells were pre-treated with AG490 or IL-6 for 2 h, and then incubated with germacrone for 24 h. The expression of p-JAK2, JAK2, p-STAT3, STAT3, p53, Bax and Bcl-2 was detected by Western blotting. The activity of HepG2 cells was tested by MTT assay. The apoptosis of HepG2 cells and levels of reactive oxygen species (ROS) were flow cytometrically measured. The results showed that germacrone exposure decreased p-STAT3 and p-JAK2 and regulated expression of p53 and Bcl-2 family members at the same time. Moreover, IL-6 enhanced the activation of the JAK2/STAT3 signalling pathway and therefore attenuated the germacrone-induced apoptosis. Suppression of JAK2/STAT3 signalling pathway by AG490, an inhibitor of JAK2, resulted in apoptosis and an increase in ROS in response to germacrone exposure. We therefore conclude that germacrone induces apoptosis through the JAK2/STAT3 signalling pathway. | 柳昀熠 郑倩 方斌 王维 马凤云 Sadia Roshan Amal Banafa 陈明洁 常俊丽 邓小敏 李克秀 杨广笑 何光源 | 2013 | Journal of Huazhong University of Science and Technology(Medical Sciences)2013，33，3： | 9 |
| 8 | Fucoidan Induces Apoptosis of HepG2 Cells by Down-regulating p-Stat3显示文摘Fucoidan is one of the main bioactive components of polysaccharides. The current study was focused on the anti-tumor effects of fucoidan on human heptoma cell line HepG2 and the possible mechanisms. Fucoidan treatment resulted in cell cycle arrest and apoptosis of HepG2 cells in a dose-dependent manner detected by MTT assay, flow cytometry and fluorescent microscopy. The results of flow cytometric analysis revealed that fucoidan induced G2/M arrest in the cell cycle progression. Hoechst 33258 and Annexin V/PI staining results showed that the apoptotic cell number was increased, which was associated with a dose-dependent up-regulation of Bax and down-regulation of Bcl-2 and p-Stat3. In parallel, the up-regulation of p53 and the increase in reactive oxygen species were also observed, which may play important roles in the inhibition of HepG2 growth by fucoidan. In the meantime,Cyclin B1 and CDK1 were down-regulated by fucoidan treatment. Down-regulation of p-Stat3 by fucoidan resulted in apoptosis and an increase in ROS in response to fucoidan exposure. We therefore concluded that fucoidan induces apoptosis through the down-regulation of p-Stat3. These results suggest that fucoidan may be used as a novel anti-cancer agent for hepatocarcinoma. | Sadia Roshan 柳昀熠 Amal Banafa 陈慧洁 李克秀 杨广笑 何光源 陈明洁 | 2014 | Journal of Huazhong University of Science and Technology(Medical Sciences)2014，34，3： | 7 |
| 9 | Preparation and properties of chitosan–metal complex:Some factors influencing the adsorption capacity for dyes in aqueous solution显示文摘Chitosan–metal complexes have been widely studied in wastewater treatment, but there are still various factors in complex preparation which are collectively responsible for improving the adsorption capacity need to be further studied. Thus, this study investigates the factors affecting the adsorption ability of chitosan–metal complex adsorbents, including various kinds of metal centers, different metal salts and crosslinking degree. The results show that the chitosan–Fe( Ⅲ) complex prepared by sulfate salts exhibited the best adsorption efficiency(100%) for various dyes in very short time duration(10 min), and its maximum adsorption capacity achieved 349.22 mg/g. The anion of the metal salt which was used in preparation played an important role to enhance the adsorption ability of chitosan–metal complex. SO_4^(2-) ions not only had the effect of crosslinking through electrostatic interaction with amine group of chitosan polymer, but also could facilitate the chelation of metal ions with chitosan polymer during the synthesis process.Additionally, the p H sensitivity and the sensitivity of ionic environment for chitosan–metal complex were analyzed. We hope that these factors affecting the adsorption of the chitosan–metal complex can help not only in optimizing its use but also in designing new chitosan–metal based complexes. | Sadia Rashid Chensi Shen Jing Yang Jianshe Liu Jing Li | 2018 | Journal of Environmental Sciences2018，30，4： | 6 |
| 10 | Concomitant-chemoradiotherapy-associated oral lesions in patients with oral squamous-cell carcinoma显示文摘Objective:Oral squamous-cell carcinoma(OSCC)accounts for >90% of oral cancers affecting adults mostly between the fourth to seventh decades of life.The most common OSCC treatment is concomitant chemoradiotherapy(CCRT)having both locoregional and distant control,but CCRT has acute and chronic toxic effects on adjacent normal tissue.This study aimed to determine the side effects of CCRT on the oral mucosa and to characterize the clinicopathology of oral lesions in patients with OSCC.Methods:This descriptive,cross-sectional study was certified by the Ethical Review Committee(UHS/Education/126-12/2728)of the University of Health Sciences,Lahore,Pakistan.OSSC patients(n=81)with various histological subtypes,grades,and stages were recruited,and findings on their oral examination were recorded.These patients received 70,90,and 119 Gy of radiotherapy dosages in combination with the chemotherapy drugs cisplatin and 5-fluorouracil.Data were analyzed using SPSS 20.0.Results:The most common presentation of OSCC was a nonhealing ulcer(63%) involving tongue(55.6%).Clinical findings included mucositis(92.6%)and xerostomia of mild,moderate,and severe degrees in 11.1%,46.9%,and 35.8% cases,respectively.Ulcers(87.7%),palpable lymph nodes(64.2%),limited mouth opening(64.2%)and fistula(40.7%) were also observed.In females,the association of radiotherapy dosage with limited mouth opening,xerostomia,and histological grading was statistically significant(P<0.05).The association of chemotherapy drugs with xerostomia(P=0.003)was also statistically significant.Conclusions:CCRT induced mucositis,xerostomia,and trismus in patients with OSCC. | Sadia Minhas Muhammad Kashif Wasif Altaf Nadeem Afzal Abdul Hanan Nagi | 2017 | Cancer Biology & Medicine2017，14，2： | 6 |
| 11 | 土壤中重金属形态分布对化学提取的影响显示文摘重金属在土壤中有多种赋存形态,其形态分布不仅决定了土壤污染的程度,也在很大程度上影响着对土壤重金属污染的修复效果。鉴于目前关于重金属的形态分布对化学提取效率影响研究不足的问题,本文以Pb、Cr实际污染的土壤(A1、B1)以及配制的污染土壤(A2、B2)为研究对象,采用改进的BCR连续提取法对土壤重金属的形态进行分析,选取氯化钙、盐酸、氢氧化钠、柠檬酸、乙二胺四乙酸二钠、谷氨酸二乙酸四钠和皂角苷7种化学提取剂对土壤重金属进行提取,对比提取前后重金属的形态分布来确定重金属形态分布对提取效率的影响。结果表明:人工配制污染土壤A2、B2中可提取态重金属的含量(88.07%、79.81%)高于实际污染土壤A1、B1(80.80%、37.55%)。EDTA(乙二胺四乙酸二钠)对A1、A2、B1、B2中重金属的提取效率最高分别为80.09%、89.80%、24.22%、53.63%,7种提取剂振荡提取结果显示,可提取形态重金属含量越高,重金属的提取率越高。对化学提取后重金属的形态进行分析,发现除B1外,EDTA和GLDA(谷氨酸二乙酸四钠)对A1、A2、B2可提取态的去除量占总去除量的比例都达到92%以上,证明了重金属整体的提取率主要是由可提取态重金属的提取率所决定的。 | 臧小龙 谷庆宝 孟柯 Sadia Qayyum 彭昌盛 | 2017 | 环保科技2017，23，1： | 6 |
| 12 | 吉马酮抑制肝癌BEL7402细胞增殖机制初步研究显示文摘目的研究吉马酮抑制肝癌Bel7402细胞增殖的作用机理。方法通过MTT法比较吉马酮对Bel7402和L02细胞增殖的影响,流式细胞仪检测并确定吉马酮对Bel7402细胞凋亡和活性氧含量的影响。Western-blot法检测吉马酮处理前后p53、Bax和Bcl-2蛋白合成的变化。结果实验浓度范围内,吉马酮可选择性浓度依赖性的抑制Bel7402细胞的增殖;吉马酮剂量依赖性的促进肝癌Bel7402细胞凋亡和上调活性氧含量;吉马酮显著促进抑癌蛋白p53和Bax的表达,抑制抗凋亡蛋白Bcl-2的表达。结论吉马酮在体外能明显抑制肝癌Bel7402细胞的增殖,上述变化可能通过上调p53和Bax、下调Bcl-2及上调活性氧含量来完成。 | Sadia Roshan 柳昀熠 郑倩 王维 方斌 杨广笑 何光源 陈明洁 张淑琴 | 2014 | 时珍国医国药2014，25，12： | 5 |
| 13 | Molecular study of HCV detection,genotypes and their routes of transmission in North West Frontier Province,Pakistan显示文摘Objective:To determine hepatitis C virus(HCV) genotypes and explore the associated risk factors in chronic HCY patients.Methods:A total of 116 patients with chronic hepatitis C were subjected to polymerase chain reaction(PCR) based detection out of which 112 chronic HCV patients[53 male(47.32%),59 female(52.68%);mean age(43.76±16.40) years;mean BMI(23.8± 3.9)kg]were enrolled in this study.The frequency of 6 HCV genotypes and associated risk factors were evaluated from five districts of North West Frontier Province(NWFP).Results:Genotype 3was the most prevalent in 73 samples(65.17%) followed by genotype 1 in 24(21.42%) and genotype2 in 13(11.60%) samples.Genotype3 had significantly high prevalence(P=0.0002).The results showed that 48(42.85%) samples were infected with HCV 3a:25(22.32%) with 3b;14(12.50%) with 1a;10(8.92%)with 1b;11(9.82%) with 2a;2(1.78%) with 2b;and 2 were untypable.The distribution of HCV genotypes in Murdan.Charsadda,Peshawar.Sawabi and Nowshehra districts was different.Use of unsterile equipment lor medication,barbers and previous history of hospitalization were the main risk factors for HCV transmission.Conclusions:Cenotype 3a and 3b.1a.1b and 2a are the common genotypes in NWFP.Genotype 4.5.and 6 can not be found in a single sample.The level of awareness about various modes ot transmission of HCV among the population is found to be verv low. | Asher Zaman Safi Yasir Waheed Joharia Sadat Solat-Ul-Islam Sadia Salahuddin Umar Saeed Muhammad Ashraf | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，7： | 5 |
| 14 | Fucoidan Induces G_1 Phase Arrest and Apoptosis through Caspases-dependent Pathway and ROS Induction in Human Breast Cancer MCF-7 Cells显示文摘Fucoidan is an active component of seaweed, which inhibits proliferation and induces apoptosis of several tumor cells while the detailed mechanisms underlying this process are still not clear.In this study, the effect of Fucoidan on the proliferation and apoptosis of human breast cancer MCF-7cells and the molecular mechanism of Fucoidan action were investigated. Viable cell number of MCF-7 cells was decreased by Fucoidan treatment in a dose-dependent manner as measured by MTT assay.Fucoidan treatment resulted in G1 phase arrest of MCF-7 cells as revealed by flow cytometry, which was associated with the decrease in the gene expression of cyclin D1 and CDK-4. Annexin V/PI staining results showed that the number of apoptotic cells was associated with regulation of cytochrome C, caspase-8, Bax and Bcl-2 at transcriptional and translational levels. Both morphologic observation and Hoechst 33258 assay results confirmed the pro-apoptotic effect of Fucoidan. Meanwhile, the ROS production was also increased by Fucoidan treatment, which suggested that Fucoidan induced oxidative damage in MCF-7 cells. The results of present study demonstrated that Fucoidan could induce G1 phase arrest and apoptosis in MCF-7 cells through regulating the cell cycle and apoptosis-related genes or proteins expression, and ROS generation is also involved in these processes. | Amal M. Banafa Sadia Roshan 柳昀熠 陈慧洁 陈明洁 杨广笑 何光源 | 2013 | Journal of Huazhong University of Science and Technology(Medical Sciences)2013，33，5： | 5 |
| 15 | ω-3多不饱和脂肪酸抗肿瘤新途径——抑制具核梭杆菌黏附宿主细胞显示文摘【背景】大量文献报道ω-3多不饱和脂肪酸尤其是二十二碳六烯酸(Docosahexaenoic Acid,DHA)与二十碳五烯酸(Eicosapentaenoic Acid,EPA)具有抗肿瘤作用,但是其抗肿瘤机制还不够完善。【目的】探究ω-3多不饱和脂肪酸、具核梭杆菌以及结直肠癌三者之间的关联。【方法】在检测二十二碳六烯酸、二十碳五烯酸、α-亚麻酸(α-Linolenic Acid,ALA)等ω-3多不饱和脂肪酸对人结直肠腺癌细胞Caco-2、正常结肠上皮细胞NCM460生长影响的基础上,检测DHA等3种多不饱和脂肪酸对具核梭杆菌黏附人体细胞以及Fap2、FadA、RadD等具核梭杆菌毒力关键基因表达的影响。【结果】30μg/mL的DHA、EPA、ALA对Caco-2生长抑制分别为9.09%、4.95%、7.52%,而对NCM460生长抑制达31.15%、25.48%、29.11%,而且相关抑制作用仅具有浓度依赖性而无时间依赖性。经30μg/mL的DHA、EPA、ALA预处理的具核梭杆菌黏附Caco-2细胞的能力分别下降81.04%(P=0)、93.63%(P=0)和68.63%(P=0);而共培养时加入DHA、EPA、ALA对具核梭杆菌黏附Caco-2细胞的能力没有显著影响。同时,30μg/mLDHA处理导致F.nucleatum的Fap2基因显著下降10.22%(P=0.027);30μg/mL EPA处理导致FadA、Fap2基因分别显著下降23.49%(P=0)、15.09%(P=0.003);30μg/mL ALA处理导致FadA基因显著下降26.75%(P=0.012)。【结论】综合上述实验结果以及DHA、EPA、ALA仅能短时间抑制具核梭杆菌生长等文献报道,我们认为,DHA、EPA等ω-3多不饱和脂肪酸并非简单地直接杀伤或抑制肿瘤细胞和F.nucleatum;抑制FadA、Fap2等黏附相关基因表达,降低F.nucleatum黏附宿主细胞能力是其抗肿瘤作用的关键组成部分。ω-3多不饱和脂肪酸等活性物质对F.nucleatum等在结直肠肿瘤发生、发展中发挥重要作用的肠道细菌的影响与机制应深入开展研究。 | 其力格尔 Sadia Nawab 范殊璇 曹博 李雨昕 田明振 张商浩 马伟 邓子新 | 2021 | 微生物学通报2021，48，3： | 4 |
| 16 | Are serum leptin levels predicted by lipoproteins, vitamin D and body composition?显示文摘BACKGROUND Both obesity and vitamin D deficiency are important health issues in Pakistan.The connection between body composition, Vitamin D and leptin in young adults is important to be studied as body composition may affect bone health and therefore the possibility of osteoporosis in later life. Few studies have attempted to investigate the effect of body composition and leptin with vitamin D in adolescence.AIM To investigate the association of serum leptin with body composition, lipids and25-hydroxyvitamin D(25 OHD) in adults.METHODS This cross-sectional study was conducted on 167 apparently healthy adults.Demographics were recorded, bioelectrical impedance analysis was performed and clinical history noted. Serum leptin was measured using DIA source kit on ELISA and total 25 OHD was measured on ADVIA-Centaur; Siemens. Total cholesterol and high density lipoprotein cholesterol were quantified using Enzymatic Endpoint Method and Cholesterol Oxidase-Phenol Aminophenazone method respectively. Biochemical analysis was done in the Departments of Pathology and Laboratory Medicine and Biological and Biomedical Sciences, Aga Khan University Hospital Karachi Pakistan.RESULTS Median age of the group(n = 167) was 20 years(IQR 27-20); 55.7% were females.Majority(89.2%, n = 149) of the study group was 25 OHD deficient, 6%(n = 10)had insufficient serum 25 OHD levels and 4.8%(n = 8) had sufficient D levels.Females, had higher median leptin levels [2.71(IQR 4.76-1.66 ng/mL)] compared to their counterparts [1.3(3.60-0.54 ng/mL), P < 0.01]. Multiple regression analysis suggested that basal metabolic rate, muscle mass, body fat percent, bone mass and serum 25 OHD were the most contributing factors to serum leptin levels. Bone mass and serum 25 OHD in fact bore a negative correlation with leptin.CONCLUSION The results indicate that basal metabolic rate, muscle mass, body fat percent, bone mass and serum 25 OHD have an impact on serum leptin. Being a cross sectional study causal relationship between leptin and other variables could not be determined. | Aysha Habib Khan Syeda Sadia Fatima Ahmed Raheem Lena Jafri | 2019 | World Journal of Diabetes2019，10，4： | 4 |
| 17 | The amazing potential of fungi:50 ways we can exploit fungi industrially显示文摘Fungi are an understudied,biotechnologically valuable group of organisms.Due to the immense range of habitats that fungi inhabit,and the consequent need to compete against a diverse array of other fungi,bacteria,and animals,fungi have developed numerous survival mechanisms.The unique attributes of fungi thus herald great promise for their application in biotechnology and industry.Moreover,fungi can be grown with relative ease,making production at scale viable.The search for fungal biodiversity,and the construction of a living fungi collection,both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products.This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology.We provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable researchers.We also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products.Fungi have provided the world with penicillin,lovastatin,and other globally significant medicines,and they remain an untapped resource with enormous industrial potential. | Kevin D.Hyde Jianchu Xu Sylvie Rapior Rajesh Jeewon Saisamorn Lumyong Allen Grace T.Niego Pranami D.Abeywickrama Janith V.S.Aluthmuhandiram Rashika S.Brahamanage Siraprapa Brooks Amornrat Chaiyasen K.W.Thilini Chethana Putarak Chomnunti Clara Chepkirui Boontiya Chuankid Nimali I.de Silva Mingkwan Doilom Craig Faulds Eleni Gentekaki Venkat Gopalan Pattana Kakumyan Dulanjalee Harishchandra Hridya Hemachandran Sinang Hongsanan Anuruddha Karunarathna Samantha C.Karunarathna Sehroon Khan Jaturong Kumla Ruvishika S.Jayawardena Jian-Kui Liu Ningguo Liu Thatsanee Luangharn Allan Patrick G.Macabeo Diana S.Marasinghe Dan Meeks Peter E.Mortimer Peter Mueller Sadia Nadir Karaba N.Nataraja Sureeporn Nontachaiyapoom Meghan O’Brien Watsana Penkhrue Chayanard Phukhamsakda Uma Shaanker Ramanan Achala R.Rathnayaka Resurreccion B.Sadaba Birthe Sandargo Binu C.Samarakoon Danushka S.Tennakoon Ramamoorthy Siva Wasan Sriprom T.S.Suryanarayanan Kanaporn Sujarit Nakarin Suwannarach Thitipone Suwunwong Benjarong Thongbai Naritsada Thongklang Deping Wei S.Nuwanthika Wijesinghe Jake Winiski Jiye Yan Erandi Yasanthika Marc Stadler | 2019 | Fungal Diversity2019，，4： | 3 |
| 18 | Chokeberry(Aronia melanocarpa)as a new functional food relationship with health:an overview显示文摘As a consumer product,black chokeberry(Aronia melanocarpa)is diffi cult to consume due to its pungent taste.It is mainly used in various food industries to produce a variety of juices,preserves,extracts,fruit teas and nutritional supplements.Traditionally,Potawatomi Native Americans used black chokeberry to treat colds,coughs and fever.It has been found to contain numerous active ingredients.Currently,there are many health and medical products that contain black chokeberry.The purpose of this review is to understand the nutritional benefi ts of black chokeberry and to explore its benefi cial effects on biological systems in vitro,thereby emphasizing its therapeutic prospects. | Yiwen Zhang Yingchun Zhao Xinglong Liu Xueyan Chen Chuanbo Ding Ling Dong Jinping Zhang Shuwen Sun Qiteng Ding Sadia Khatoom Zhiqiang Cheng Wencong Liu Liqian Shen Fengyan Xiao | 2021 | Journal of Future Foods2021，1，2： | 3 |
| 19 | 水稻OsNCED4基因的克隆及功能初探显示文摘脱落酸(abscisic acid,ABA)是一种抑制植物生长的植物激素,因能促使叶子脱落而得名,在植物逆胁迫中发挥关键作用。由NCED基因编码的9-顺-环氧类胡萝卜素双加氧酶(NCED),是ABA在高等植物中的关键酶。本研究已成功从水稻Ilmibyeo(O.sativa L.ssp.japonica)基因组中克隆得到Os NCED4,生物信息学分析表明,Os NCED4基因位于水稻第7号染色体上,ORF全长1749 bp,无内含子结构,编码582个氨基酸,具有NCED家族的RPE65保守结构;以该基因和其启动子序列构建组织表达载体pOsNCED4::GUS、pCaMV35S::OsNCED4:EGFP和RNAi干扰载体p Ca MV35S::OsNCED4:RNAi,用于基因表达和功能分析,并利用农杆菌介导的方法,成功获得了相应遗传载体的转基因植株;GUS染色结果表明,该基因在叶片、叶耳、小穗等组织部位均有表达;RT-PCR结果显示,Os NCED4在水稻不同生育期的根、茎、叶、叶鞘和穗子中均有不同程度的表达,但表达量具有明显的差异;亚细胞定位结果显示,OsNCED4基因编码的蛋白定位于细胞核;与野生型植株相比,RNAi植株的花粉育性无明显差异,而结实率显著下降。该研究结果为进一步研究水稻OsNCED4基因表达的调控,以及为水稻生长发育上的功能研究提供了科学依据。 | 周丽 李梦婷 朱骞 Sadia Nadir 李伟 郭效琼 冯天 陈丽娟 李东宣 | 2020 | 分子植物育种2020，18，7： | 3 |
| 20 | Towards Collaborative Robotics in Top View Surveillance:A Framework for Multiple Object Tracking by Detection Using Deep Learning显示文摘Collaborative Robotics is one of the high-interest research topics in the area of academia and industry.It has been progressively utilized in numerous applications,particularly in intelligent surveillance systems.It allows the deployment of smart cameras or optical sensors with computer vision techniques,which may serve in several object detection and tracking tasks.These tasks have been considered challenging and high-level perceptual problems,frequently dominated by relative information about the environment,where main concerns such as occlusion,illumination,background,object deformation,and object class variations are commonplace.In order to show the importance of top view surveillance,a collaborative robotics framework has been presented.It can assist in the detection and tracking of multiple objects in top view surveillance.The framework consists of a smart robotic camera embedded with the visual processing unit.The existing pre-trained deep learning models named SSD and YOLO has been adopted for object detection and localization.The detection models are further combined with different tracking algorithms,including GOTURN,MEDIANFLOW,TLD,KCF,MIL,and BOOSTING.These algorithms,along with detection models,help to track and predict the trajectories of detected objects.The pre-trained models are employed;therefore,the generalization performance is also investigated through testing the models on various sequences of top view data set.The detection models achieved maximum True Detection Rate 93%to 90%with a maximum 0.6%False Detection Rate.The tracking results of different algorithms are nearly identical,with tracking accuracy ranging from 90%to 94%.Furthermore,a discussion has been carried out on output results along with future guidelines. | Imran Ahmed Sadia Din Gwanggil Jeon Francesco Piccialli Giancarlo Fortino | 2021 | IEEE/CAA Journal of Automatica Sinica2021，8，7： | 3 |
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