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| 1 | Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines:Role of cytokines显示文摘AIM:To study KRAS/BRAF mutations in colorectalcancer(CRC)that influences the efficacy of treatment.To develop strategies for overcoming combination of treatment.METHODS:Five colonic cell-lines were investigated:DLD-1 with KRAS(G13D)mutation,HT 29 and Colo205 with BRAF(V600E)mutation as well as the wild type(Wt)cell-lines Caco2 and Colo-320.DLD-1(KRAS),HT-29(BRAF)and Caco2(Wt)cell lines were treated with cytokines(TNFα50 ng,IL-1β1 ng and IFNγ50ng)and harvested at different time points(1-24 h).KRAS inhibition was performed by the siRNA-approach.Two colorectal cancer cells DLD-1 and Caco2 were used for KRAS inhibition.About 70%confluency were confirmed before transfection with small interferring RNA(siRNA)oligonucleotides.All the synthetic siRNA sequences were designed in our laboratory.Total RNA and protein was isolated from the cells for RT-PCR and Western blotting.Densitometry of the Western blotting was analyzed with the Image J software(NIH).Results are shown as mean±SD.RESULTS:RT-PCR analysis in non-stimulated cells showed a low basal expression of TNFαand IL-1βin the DLD-1 KRAS-mutated cell-line,compared to Caco2wild type.No detection was found for IL-6 and IFNγin any of the studied cell lines.In contrast,pro-angiogenic chemokines(CXCL1,CXCL8)showed a high constitutive expression in the mutated cell-lines DLD-1(KRAS),HT-29 and Colo205(BRAF),compared to wild type(Caco2).The anti-angiogenic chemokine(CXCL10)showed a high basal expression in wild-type,compared to mutated cell-lines.KRAS down-regulation by siRNA showed a significant decrease in CXCL1 and CXCL10gene expression in the DLD-1(KRAS)cell-line in comparison to wild type(Caco2)at 72 h after KRAS silencing.In contrast,the specific KRAS inhibition resulted in an up-regulation of CXCL1 and CXCL10.The results of our study show a higher expression of pro-angiogenic chemokines at basal level in mutated cell-lines,which was further increased by cytokine treatment.CONCLUSION:To summarize,basal chemokine gene expression for pro-angiogenic chemokines was high in mutated as compared to wild type cell-lines.This reflects the likely existence of a different microenvironment in tumours consistent of wild type or mutated cells.This may help to rationalize the choice of molecular targets for suitable therapeutic investigation in clinical studies. | Sajjad Khan Silke Cameron Martina Blaschke Federico Moriconi Naila Naz Ahmad Amanzada Giuliano Ramadori Ihtzaz Ahmed Malik | 2014 | World Journal of Gastroenterology2014，20，11： | 6 |
| 2 | Identification of Rice QTLs for Important Agronomic Traits with Long-Kernel CSSL-Z741 and Three SSSLs显示文摘Rice kernel shape affects kernel quality(appearance) and yield(1000-kernel weight) and therefore is an important agronomic trait, but its inheritance is complicated. We identified a long-kernel rice chromosome segment substitution line(CSSL), Z741, derived from Nipponbare as a recipient and Xihui 18 as a donor parent. Z741 has six substitution segments distributed on rice chromosomes 3, 6, 7, 8 and 12 with an average replacement length of 5.82 Mb. Analysis of a secondary F2 population from a cross between Nipponbare and Z741 identified 20 QTLs for important agronomic traits. The kernel length of Z741 is controlled by a major QTL(qKL3) and a minor QTL(qKL7). Candidate gene prediction and sequencing indicated that qKL3 may be an allele of OsPPKL1, which encodes a protein phosphatase implicated in brassinosteroid signaling, and qKL7 is an unreported QTL. Finally, we validated eight QTLs(qKL3, qKL7, qRLW3-1, qRLW7, qPH3-1, qKWT3, qKWT7 and qNPB6) using three selected singlesegment substitution lines(SSSLs), S1, S2 and S3. Also, we detected five QTLs(qKL6, qKW3, qKW7, qKW6 and qRLW6) in S1, S2 and S3, which were not found in the Nipponbare/Z741 F2 population. However, qNPB3, qNPB7 and qPL3 QTLs were not validated by the three SSSLs in 2019, suggesting that minor QTLs are susceptible to environmental factors. These results lay the foundation for studying the biodiversity of kernal length and molecular breeding of different kernel types. | Wang Hui Zhang Jiayu Naz Farkhanda Li Juan Sun Shuangfei He Guanghua Zhang Ting Ling Yinghua Zhao Fangming | 2020 | Rice science2020，27，5： | 5 |
| 3 | Seedling-lump integrated non-destructive monitoring for automatic transplanting with Intel RealSense depth camera显示文摘Non-destructive plant growth parameters measurement is an important concern in automatic-seedling transplanting.Recently,several image-basedmonitoring approaches have been proposed and potentially developed for several agricultural applications.The presented study proposed and developed a RealSense-based machine vision system for the close-shot seedling-lump integrated monitoring.The strategy was based on the close-shot depth information.Further,the point cloud clustering and suitable algorithms were applied to obtain the segmentation of 3D seedling models.In addition,the data processing pipeline was developed to assess the differentmorphological parameter of 4 different seedling varieties.The experiments were carried out with 4 different seedling varieties(pepper,tomato,cucumber,and lettuce)and trained under different light conditions(light and dark).Moreover,analysis results showed that therewas not significantly different(p<0.05)found towards light and dark environments due to close-shot near-infrared detection.However,the results revealed that the stem diameter relationship between RealSense and the manual method was found for R^2=0.68 cucumber,R^2=0.54 tomato,R^2=0.35 pepper,and R^2=0.58 lettuce seedlings.Whereas,the seedling height relationship between RealSense and the manual methodwas found higher than R^2=0.99,0.99,0.99,and 0.99 for pepper,tomato,cucumber,and lettuce,respectively.Based on the experiment results,it was concluded that the RGB-D integrated monitoring system with the purposed method could be practiced for nursery seedlings most promisingly without high labour requirements in terms of ease of use.The system revealed a good sturdiness and relevance for plant growth monitoring.Additionally,it has the perspective for future practical value to real-time vision servo operations for transplanting robots. | Tabinda Naz Syed Liu Jizhan Zhou Xin Zhao Shengyi Yuan Yan Sami Hassan Ahmed Mohamed Imran Ali Lakhiar | 2019 | Artificial Intelligence in Agriculture2019，，3： | 4 |
| 4 | Adansonia digitata L.(baobab):a review of traditional information and taxonomic description显示文摘Adansonia digitata L.(Malvaceae) is commonly known as baobab tree native to Africa.Baobab is a multi-purpose tree which offers protection and provides food,clothing and medicine as well as raw material for many useful items.The fruit pulp,seeds,leaves,flowers.roots,and bark of baobab are edible and they have been studied by scientists for their useful properties.The fruit pulp have very high vitamin C.calcium,phosphorus,carbohydrates,fibers,potassium,proteins and lipids content,which can be used in seasoning as an appetizer and also make juices.Seeds contain appreciable quantities of phosphorus,magnesium,zinc,sodium,iron,manganese,whereas they have high levels of lysine,thiamine,calcium and iron.Baobab has numerous biological properties including antimicrobial,anti-malarial,diarrhoea,anaemia,asthma,antiviral,anti-oxidant and anti-inflammatory activities amongst others.Phytochemical investigation revealed the presence of flavonoids.phytosterols.amino acids,fatty acids,vitamins and minerals.The review summarizes the information on various aspects of traditional information,taxonomic description,medicinal properties and importantly nutritional value. | Jitin Rahul Manish Kumar Jain Shishu Pal Singh Rakesh Kant Kama Anuradha Aliya Naz Anup Kumar Gupta Sujeet Kumar Mrityunjay | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，1： | 4 |
| 5 | Antimicrobial potential of Ricinus communis leaf extracts in different solvents against pathogenic bacterial and fungal strains显示文摘Objective:To investigate the in vitro antimicrobial activities of the leaf extract in different solvents viz.,methanol,ethanol and water extracts of the selected plant Ricinus communis.Methods:Ager well diffusion method and agar tube dilution method were carried out to perform the antibacterial and antifungal activity of methanol,ethanol and aqueous extracts.Results:Metbanol leaf extracts were found to be more active against Gram positive bacteria(Bacillus subtilis:ATCC 6059 and Staphylococcus aureus:ATCC 6538)as well as Gram negative bacteria(Pseudomonas aeruginosa:ATCC 7221 and Klebsiella pneumoniae)than ethanol and aqueous leaf extracts.Antifungal activity of methanol and aqueous leaf extracts were also carded out against selected fungal strains as Aspergillus fumigatus and Aspergillus flavus.Methanolic as well as aqueous leaf extracts of Ricinus communis were effective in inhibiting the fungal growth.Conclusions:The efficient antibacterial and antifungai activity of Ricinus communis from the present investigation revealed that the methanol leaf extracts of the selected plant have significant potential to inhibit the growth of pathogenic bacterial and fungal strains than ethanol and aqueous leaf extracts. | Rabia Naz Asghari Bano | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，12： | 4 |
| 6 | A Reliable Stochastic Numerical Analysis for Typhoid Fever Incorporating With Protection Against Infection显示文摘In this paper,a reliable stochastic numerical analysis for typhoid fever incorporating with protection against infection has been considered.We have compared the solutions of stochastic and deterministic typhoid fever model.It has been shown that the stochastic typhoid fever model is more realistic as compared to the deterministic typhoid fever model.The effect of threshold number T*hold in stochastic typhoid fever model.The proposed framework of the stochastic non-standard finite difference scheme(SNSFD)preserves all dynamical properties like positivity,bounded-ness and dynamical consistency defined by Mickens,R.E.The stochastic numerical simulation of the model showed that increase in protection leads to low disease prevalence in a population. | Muhammad Shoaib Arif Ali Raza Muhammad Rafiq Mairaj Bibi Rabia Fayyaz Mehvish Naz Umer Javed | 2019 | Computers, Materials & Continua2019，，6： | 3 |
| 7 | 心脏细胞衰老与Uvrag基因的缺失显示文摘背景:前期工作发现自噬相关基因Uvrag缺失导致年龄相关心肌病,但细胞衰老在此过程中的作用尚不清楚。目的:研究Uvrag基因缺失对心脏细胞衰老的影响。方法:从Uvrag基因缺失纯合子小鼠和野生型小鼠中各取14只雄性小鼠分两批分别饲养到3月龄和8月龄。采用real time RT-PCR检测小鼠心脏组织衰老相关分泌表型相关因子mRNA表达变化,苏木精-伊红染色、天狼猩红染色、衰老相关β-半乳糖苷酶染色进行心肌组织学观察,透射电镜观察小鼠心肌细胞超微结构的变化,蛋白免疫印迹检测小鼠心脏组织p53蛋白表达量。实验方案经上海交通大学动物实验伦理委员会批准。结果与结论:①苏木精-伊红染色和天狼猩红染色结果显示,Uvrag基因缺失导致单个心肌细胞显著增大及心脏纤维化;②透射电镜显示,Uvrag基因缺失小鼠心肌细胞线粒体形态异常、排列紊乱,肌浆网肿胀;③衰老相关β-半乳糖苷酶染色发现,Uvrag基因缺失小鼠心脏衰老细胞显著增多;④real time RT-PCR结果表明,Uvrag基因缺失小鼠心脏衰老相关分泌表型因子表达显著上调;⑤Western blot结果显示Uvrag基因缺失导致心脏组织衰老调控关键蛋白p53表达显著升高;⑥结果说明,自噬相关基因Uvrag缺失促进小鼠心脏细胞衰老;Uvrag基因是潜在的延缓心脏衰老及抗衰老相关心脏疾病的靶分子。 | 来帅威 张沙沙 刘晓芸 Haniya Mazhar Amber Naz 贺林 朱洪新 | 2021 | 中国组织工程研究2021，25，14： | 3 |
| 8 | UVRAG基因缺失促进饥饿诱导的肝脏脂肪变性显示文摘[目的]紫外线辐射耐受相关(ultraviolet resistance-associated gene,UVRAG)是一个自噬相关基因,具有多种生物学功能.利用UVRAG基因缺失的小鼠证实了该基因缺失可阻断体内自噬流,并鉴于自噬对脂代谢有一定调控作用,研究了UVRAG在饥饿诱导的肝脏脂肪变性中的作用.[方法与结果]野生型小鼠和UVRAG基因缺失小鼠给予正常饮食或24 h饥饿处理.在正常生理条件下,油红染色显示UVARG基因缺失小鼠和野生型小鼠肝脏无明显脂质积聚;但是在24 h饥饿条件下,该基因缺失可导致肝脏脂质积聚显著增多,且UVRAG基因缺失小鼠肝脏甘油三酯、游离脂肪酸质量比显著升高,而总胆固醇质量比无显著性差异.实时荧光定量聚合酶链式反应(reverse transcription-polymerize chain reaction,RT-PCR)显示,在正常生理条件下的胆固醇调节元件结合蛋白-1(sterol regulatory element-binding protein1,SREBP-1)基因在UVRAG基因缺失小鼠肝脏表达显著下调,但在饥饿条件下,UVARG基因缺失小鼠和野生型小鼠肝脏SREBP-1表达无显著差异;而在实验组和对照组中其他肝脏脂代谢相关基因CD 36,FAS,CPT-1,PPAR和FGF 21的表达无显著性变化.此外,UVARG基因缺失对脂代谢调控分子AMPK活性无显著影响.在饥饿条件下,UVRAG基因缺失可导致血浆甘油三酯和游离脂肪酸浓度上升,血糖浓度下降.[结论]UVARAG基因缺失促进饥饿诱导的肝脏脂肪变性,这可能与肝脏自噬阻断和肝脏摄取血浆游离脂肪酸增多有关. | 胡晓雯 张沙沙 安琳 AMBER Naz 朱洪新 | 2018 | 上海大学学报(自然科学版)2018，24，3： | 2 |
| 9 | Single-Dose Gamma-Irradiation Induces Up-Regulation of Chemokine Gene Expression and Recruitment of Granulocytes into the Portal Area but Not into Other Regions of Rat Hepatic Tissue显示文摘 | Ihtzaz Ahmed Malik Federico Moriconi Nadeem Sheikh Naila Naz Sajjad Khan Jozsef Dudas Tümen Mansuroglu Clemens Friedrich Hess Margret Rave-Fr?nk Hans Christiansen Giuliano Ramadori | 2010 | The American Journal of Pathology2010，，4： | 2 |
| 10 | Removal of lead and cadmium ions by single and binary systems using phytogenic magnetic nanoparticles functionalized by 3-marcaptopropanic acid显示文摘The present research study is focused on green fabrication of superparamagnetic Phytogenic Magnetic Nanoparticles(PMNPs), and then its surface functionalization with 3-Mercaptopropionic acid(3-MPA). The resulting material(i.e. 3-MPA@PMNPs) characterized by FTIR, powder XRD, SEM, TEM, EDX, VSM, BET and TGA techniques and then further employed for the investigation of the adsorptive removal of lead(Pb^2+) and cadmium(Cd^2+) ions from aqueous solutions in single and binary systems. The material showed fastest adsorptive rate(98.23%) for Pb^2+ and(96.5%) Cd2+within the contact time of 60 min at pH 6.5 in the single system. The experimental data were fitted well to Langmuir isotherm, indicated monolayer adsorption of both metal ions onto 3-MPA@PMNPs and an estimated comparable adsorptive capacity of 68.41 mg·g^-1(Pb2+) and 79.8 mg·g^-1(Cd2+) at p H 6.5. However, kinetic data agreed well with pseudo-second-order model, and indicated that the removal mainly supported chemisorption and/or ion-exchange mechanism. Thermodynamic parameters such asΔGo,ΔHo, and ΔSo, were-3259.20, 119.35 and 20.73 for Pb^2+, and-1491.10, 45.441 and 7.87 for Cd^2+ at temperature 298.15 K, confirmed that adsorption was endothermic, spontaneous and favorable. The material demonstrated higher selectivity of Pb2+ and its removal efficiency was(98.20 ± 0.3)% in binary system experiments. The material persisted performance up-to seven(07) consecutive treatment cycles without losing their stability and offered comparable fastest magnetic separation(35 s) from aqueous solutions. Therefore, it is recommended that the prepared material can be employed to remove toxic heavy metal ions from water/wastewaters and this 'green' method can easily be implemented at large scale in low economy countries. | Imran Ali Changsheng Peng Iffat Naz | 2019 | Chinese Journal of Chemical Engineering2019，27，4： | 2 |
| 11 | Sedative and antinociceptive activities of two new sesquiterpenes isolated from Ricinus communis显示文摘Two new sesquiterpenes, trivially named ricinusoids A(1) and ricinusoids B(2), were isolated from ethyl acetate fraction of Ricinus communis. The structures of new compounds were elucidated by detailed spectroscopic techniques, including 1 D-and 2 D-NMR, UV, IR spectroscopy, and mass spectrometry. The compounds(1-2) were also assessed for in-vivo sedative and analgesic like effects in open field and acetic acid induced writhing tests respectively at 5, 10, and 20 mg·kg^(–1) i.p. Pretreatment of both test compounds caused significant(P ≤ 0.05) reduction in locomotive activity like sedative agents and abdominal constrictions like analgesics. Both compounds(1-2) possessed marked sedative and antinociceptive effects in animal models. | Umar Farooq Ajmal Khan Sadia Naz Abdur Rauf Haroon Khan Afsar Khan Irfan Ullah Syed Majid Bukhari | 2018 | Chinese Journal of Natural Medicines2018，16，3： | 2 |
| 12 | The influence of age at symptom onset and length of follow up on mortality in patients with recent-onset inflammatory polyarthritis 显示文摘 | Naz SM Farragher TM Bunn DK | 2008 | Arthritis Rheum2008，58，4： | 1 |
| 13 | Oxidative stability of olive, corn and soybean oil under different conditions显示文摘 | NAZ S SHEIKH H SIDDIQI R | 2004 | Food Chemistry2004，88，： | 1 |
| 14 | Protection from cyclophosphamide-induced ovarian damage with bone marrow-derived mesenchymal stem cells during puberty显示文摘 | Kilic S Pinarli F Ozogul C Tasdemir N Naz Sarac G Delibasi T | 2014 | Gynecol Endocrinol2014，30，2： | 1 |
| 15 | Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men显示文摘 | Naz R K Chauhan S C | 2001 | Mol Hum Reprod2001，7，1： | 1 |
| 16 | Expression of Alpha and Gamma Interferon Receptors in the Sperm Cell显示文摘 | Rajeshk NAZ Subhash C Chauhan | 2000 | Molecular Reproduction And Development2000，56，： | 1 |
| 17 | Iron and Cobalt Complexes of 4 - Alkyl - 2, 6 - Diiminopyri- dine Ligands: Synthesis and Ethylene Polymerization Ca- talysis 显示文摘 | CAOMPORA P J NAZ A M PALMA R P | 2008 | European Journal of Inorganic Chemistry2008，，11： | 1 |
| 18 | Antibacterial activity directed isolation of compounds frompunica granatum显示文摘 | NAZ S SIDDIQI R AHMAD S | 2007 | J Food Sci2007，72，9： | 1 |
| 19 | Recent progress toward development of vaccines against conception 显示文摘 | Naz RK | 2014 | Expert Rev Vaccines2014，13，1： | 1 |
| 20 | Investigation of fire behavior of rigid polyurethane foams containing fly ash and intumescent flame retardant by using a cone calorimeter 显示文摘 | Naz m U | 2012 | J Appl Polym Sci2012，124，4： | 1 |
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