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| 1 | 四甲基氯化铵在PCR扩增小麦基因中的关键作用显示文摘利用高简并性引物,用PCR法从小麦DNA或cDNA中合成小麦几丁质酶基因、葡聚糖酶基因和苯丙氨酸解氨酶基因片段。在PCR反应中添加四甲基氯化铵(TMACl)是合成这些特异基因片段的关键。合成的PCR片段都经末端补齐和磷酸化后用于克隆。核酸序列分析证实,这些PCR产物分别与用于设计PCR引物的基因具有高度的同源性。 | 廖玉才 李和平 Rainer Fischer | 1997 | 遗传1997，19，2： | 8 |
| 2 | 重组色氨酸脱羧酶在烟草不同亚细胞区室的表达(英文)显示文摘将萜烯类吲哚生物碱代谢关键酶———色氨酸脱羧酶 (TDC)的编码基因转到烟草 (NicotianatabacumL .)植物体内 ,标定在不同的亚细胞区室表达。通过蛋白免疫印迹法和色胺在植物体内的累积量测定分析 ,对转基因植物进行筛选。结果表明 ,TDC在叶绿体和胞液中高效表达 ,TDC在叶绿体中的表达水平最高 ,高于在胞液中的表达 ,在内质网和液泡中表达水平很低 ,用蛋白免疫印迹法未检出。 | 王淼 李秋荣 Stefano Di FIORE Rainer FISCHER | 2002 | Acta Botanica Sinica2002，44，3： | 2 |
| 3 | MEGAP impedes cell migration via regulating actin and microtubule dynamics and focal complex formation显示文摘 | Ying Yang Marco Marcello Volker Endris Rainer Saffrich Roger Fischer Michael F. Trendelenburg Rolf Sprengel Gudrun Rappold | 2006 | Experimental Cell Research2006，，12： | 1 |
| 4 | Molecular farming of pharmaceutical proteins显示文摘 | Rainer Fischer Neil Emans | 2000 | Transgenic Research (-)2000，，4： | 1 |
| 5 | One Hundred Thirty-Two Consecutive Pediatric Liver Transplants Without Hospital Mortality: Lessons Learned and Outlook for the Future显示文摘 | Dieter C. Broering Jong-Sun Kim Teresa Mueller Lutz Fischer Rainer Ganschow Turan Bicak Lars Mueller Christian Hillert Christian Wilms Bernd Hinrichs Knut Helmke Werner Pothmann Martin Burdelski Xavier Rogiers | 2004 | Annals of Surgery2004，，6： | 1 |
| 6 | Antibody-Based Resistance to Plant Pathogens显示文摘 | Stefan Schillberg Sabine Zimmermann Mei-Yun Zhang Rainer Fischer | 2001 | Transgenic Research2001，，1： | 1 |
| 7 | Detection of patients with coronary artery disease using cardiac magnetic field mapping at rest显示文摘 | Andrej Gapelyuk Niels Wessel Robert Fischer Udo Zacharzowsky Lydia Koch Daniela Selbig Henry Schütt Bianca Sawitzki Friedrich C. Luft Rainer Dietz Alexander Schirdewan | 2007 | Journal of Electrocardiology2007，，5： | 1 |
| 8 | Ohmic contacts on p-GaN : investigation of different contact metals and their thermal treatmen显示文摘 | Wenzel R Fischer G Rainer S F | 2001 | Materials Science in Semiconductor Processing2001，4，4： | 1 |
| 9 | Molecular farming of human tissue transglutaminase in tobacco plants显示文摘 | Angela Sorrentino Stefan Schillberg Rainer Fischer | 2009 | Amino Acids2009，36，： | 1 |
| 10 | The stage‐specific in vitro efficacy of a malaria antigen cocktail provides valuable insights into the development of effective multi‐stage vaccines显示文摘 | Holger Spiegel Alexander Boes Robin Kastilan Stephanie Kapelski Güven Edgue Veronique Beiss Ivana Chubodova Matthias Scheuermayer Gabriele Pradel Stefan Schillberg Andreas Reimann Rainer Fischer | 2015 | Biotechnology Journal2015，，： | 1 |
| 11 | Molecular farming of pharmaceutical proteins显示文摘 | Rainer Fischer | 2000 | Transgenic research:2000，9，4： | 1 |
| 12 | Ohmic contacts on p-GaN (Part II): Impact of semiconductor fabrication and surface treatment显示文摘 | WENZEL R FISCHER G G RAINER S F | 2001 | Materials Science in Semiconductor Processing2001，4，4： | 1 |
| 13 | Ohmic contacts on p-GaN (Part I): investigation of different contact metals and their thermal treatment显示文摘 | WENZEL R FISCHER G G RAINER S F | 2001 | Materials Science in Semiconductor Processing2001，4，4： | 1 |
| 14 | Subretinal Hemorrhages Associated with Age-Related Macular Degeneration in Patients Receiving Anticoagulation or Antiplatelet Therapy显示文摘 | Claudia Kuhli-Hattenbach Ina Barbara Fischer Rainer Schalnus Lars-Olof Hattenbach | 2010 | American Journal of Ophthalmology2010，，2： | 1 |
| 15 | Oculo-auriculo-vertebral spectrum (OAVS): clinical evaluation and severity scoring of 53 patients and proposal for a new classification显示文摘 | Christiane Tasse Stefan B?hringer Sven Fischer Hermann-Josef Lüdecke Beate Albrecht Denise Horn Andreas Janecke Rainer Kling Rainer K?nig Birgit Lorenz Frank Majewski Elisabeth Maeyens Peter Meinecke Beate Mitulla Christopher Mohr Monika Preischl Horst Um | 2005 | European Journal of Medical Genetics2005，，4： | 1 |
| 16 | Moleeular farming of recombinant in plants 显示文摘 | Rainer Fischer Yu-Cai Liao Kurt Hoffman | 1999 | Biol Chem1999，380，： | 1 |
| 17 | Ohmic Contacts on p-GaN(park 1):Investigation of Different Contact Metals and Their Thermal Treatment显示文摘 | Wenzel R Fischer G G Rainer S F | 2001 | Materials Science in Semiconductor Processing2001，，4： | 1 |
| 18 | Comparative survey of plastid and mitochondrial targeting properties of transcription factors in Arabidopsis and rice显示文摘 | Rainer Schwacke Karsten Fischer Bernd Ketelsen Karin Krupinska Kirsten Krause | 2007 | Molecular Genetics and Genomics2007，，6： | 1 |
| 19 | Outcome of liver re‐transplantation in children—Impact and special analysis of early re‐transplantation显示文摘 | Uta Herden Rainer Ganschow Enke Grabhorn Andrea Briem‐Richter Bjoern Nashan Lutz Fischer | 2014 | Pediatr Transplantation2014，，4： | 1 |
| 20 | Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls显示文摘The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods.Lignin peroxidase(LiP)from Phanerochaete chrysosporium is a hemecontaining lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules,including industrial dyes.This enzyme is therefore ideal as a starting point for protein engineering.Accordingly,we subjected two positions(165 and 264)in the environment of the catalytic Trp171 residue to saturation mutagenesis,and the resulting library of 104 independent clones was expressed on the surface of yeast cells.This yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more efficiently.We identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes(Evans blue,amido black 10B and Guinea green)and up to 13-fold higher catalytic activity.Additionally,cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis,achieving significant increases in both enzyme activity and stability compared to a whole-cell biocatalyst.LiPcoated cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 h.The best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles. | Karla IlićĐurđić Raluca Ostafe Olivera Prodanović AleksandraĐurđevićĐelmaš Nikolina Popović Rainer Fischer Stefan Schillberg Radivoje Prodanović | 2021 | Frontiers of Environmental Science & Engineering2021，15，2： | 1 |
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