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| 1 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 2 | The prognostic and therapeutic implications of circulating tumor cell phenotype detection based on epithelial-mesenchymal transition markers in the first-line chemotherapy of HER2-negative metastatic breast cancer显示文摘Background:Epithelial-mesenchymal transition(EMT)is implicated in the metastatic process and presents a chal-lenge to epithelial cell adhesion molecule-based detection of circulating tumor cells(CTCs),which have been demon-strated to be a prognostic indicator in metastatic breast cancer.Although evidence has indicated that heterogeneity of CTCs based on EMT markers is associated with disease progression,no standard recommendations have been established for clinical practice.This study aimed to evaluate the prognostic significance of dynamic CTC detection based on EMT for metastatic breast cancer patients.Methods:We enrolled 108 human epidermal growth factor receptor 2-negative metastatic breast cancer patients from the prospective phase III CAMELLIA study and applied the CanPatrol CTC enrichment technique to identify CTC phenotypes(including epithelial CTCs,biphenotypic epithelial/mesenchymal CTCs,and mesenchymal CTCs)in peripheral blood samples.Receiver operating characteristic curve analyses of total CTC count and the proportion of mesenchymal CTCs for predicting the 1-year progression-free survival(PFS)rate were conducted to determine the optimal cut-off values,and Kaplan-Meier analysis and Cox proportional hazards regression analysis were performed to investigate the prognostic value of the cut-off values of both total CTC count and the proportion of mesenchymal CTCs in combination.Results:For predicting the 1-year PFS rate,the optimal cut-off value of total CTC count was 9.5(Area under the curve[AUC]=0.538,95%confidence interval[CI]=0.418-0.657),and that of the proportion of mesenchymal CTCs was 10.7%(AUC=0.581,95%CI=0.463-0.699).We used the two cut-off values in combination to forecast PFS in which the total CTC count was equaled to or exceeded 10/5 mL with the proportion of mesenchymal CTCs surpassed 10.7%.Patients who met the combined criteria had significantly shorter median PFS than did those who did not meet the criteria(6.2 vs.9.9 months,P=0.010).A nomogram was constructed based on the criteria and significant clinicopatho-logical characteristics with a C-index of 0.613(P=0.010).Conclusions: The criteria, which combine the total CTC count and the proportion of mesenchymal CTCs, may be used to monitor therapeutic resistance and predict prognosis in patients with metastatic breast cancer. | Xiuwen Guan Fei Ma Chunxiao Li Shiyang Wu Shangying Hu Jiefen Huang Xiaoying Sun Jiayu Wang Yang Luo Ruigang Cai Ying Fan Qiao Li Shanshan Chen Pin Zhang Qing Li Binghe Xu | 2019 | Cancer Communications2019，39，1： | 15 |
| 3 | Human γδT-cell subsets and their involvement in tumor mmunity显示文摘 | Dang W u Pin Wu Fuming Qiu Qichun Wei Jian Huang | 2017 | Cellular & Molecular Immunology2017，14，3： | 11 |
| 4 | Effect of Arctium lappa L.in the dextran sulfate sodium colitis mouse model显示文摘AIM:To analyze the possible protective role of Arctium lappa L.(AL)in a murine model of ulcerative colitis(UC).METHODS:BALB/c mice were administered 100 mg/kg AL powder orally each day.After 7 d,colitis was induced by administration of dextran sulfate sodium(DSS)(5% W/V)in drinking water for a further 8 consecutive days.Diarrhea and bloody stools as well as colonic histology were observed.The level of interleukin-6(IL-6)and tu-mor necrosis factor-α(TNF-α)in colonic sections were detected by immunohistochemistry.RESULTS:There were significant differences in mean body weight values and disease activity indices between controls and AL-treated animals.Moreover,the histological findings showed that AL treatment can prevent mucosal edema,submucosal erosions,ulceration,inflammatory cell infiltration and colon damage.In addition,immunohistochemistry analysis showed that the levels of the inflammatory cytokines,IL-6 and TNF-α were also decreased in AL-treated groups.CONCLUSION:We suggest that AL can prevent intestinal damage and decrease inflammatory cytokines in mice with DSS-induced colitis.Thus,AL could prove to be a useful food for UC. | Tzou-Chi Huang Shinn-Shyong Tsai Li-Fang Liu Yu Lin Liu Hung-Jen Liu Kuo Pin Chuang | 2010 | World Journal of Gastroenterology2010，16，33： | 10 |
| 5 | 垃圾气化分析及其对中国实现碳中和与无废城市建设的贡献显示文摘气化是实现化学利用碳资源(尤其是煤)生产化学品和燃料的关键工艺。目前,全球拥有超过272套气化装置,主要应用于煤化工,尤其在中国。由于在一个化工厂通过技术集成达到工业规模的集中生产可实现减少二氧化碳排放并促进碳密集型行业的循环经济,如废物管理、化学和交通行业,因此,近年来全球对垃圾气化的兴趣日益浓厚,尤其是那些在煤气化技术方面积累了丰富的人力资本和运营经验以及拥有完整Coal-to-X产品价值链的国家,如中国,在挖掘通过垃圾气化实现Waste-to-X的潜力方面处于独特的地位。具体而言,废弃物可用于补充煤气化生产如下产品:(1)具有较低或零碳足迹的化学品,具体取决于废物是来源于化石资源还是生物资源。(2)来自生物废弃物的零碳足迹的绿色“氢”,以补充或替代来自煤气化或蒸汽重整得到的“灰色”氢,以供给氢能汽车,并推动工业脱碳。(3)来自生物废弃物的CO_(2)中性合成液体燃料,用于交通工具,以补充或替代石油燃料和合成煤制液体燃料。然而,迄今为止,利用垃圾作为气化原料的操作经验有限,并且文献中也很少涉及以前和当前的工业垃圾气化经验。为了填补这些空白,本研究介绍了1980年代至2000年代德国的两项开创性技术发展—贝伦拉特(Berrenrath)和SVZ黑水泵(即Sekundärrohstoff-Verwertungszentrum:黑水泵二次原料回收中心),并分享了以下三种垃圾与煤共气化技术(即Waste&Coal-to-X)的相关认识和经验教训:固定床气化:鲁奇干法排渣技术以及BGL熔渣气化技术。流化床气化:高温温克勒(HTW-High Temperature Winkler)气化工艺。气流床气化:GSP(Gaskombinat Schwarze Pumpe),即西门子气化工艺。此外,本研究也总结了目前全球100%垃圾气化技术的发展,即Waste-to-X。在气化技术的三种类型中,研发领域和工业界均对流化床与气流床(作为后期气化)技术的结合产生了浓厚的兴趣,这里介绍三个相关技术的重大发展—Ebara-Ube、Enerkem和ThermoChem Recovery International的技术。除此之外,固定床气化工艺的发展也得到了关注,本文介绍了两个相关技术的重大发展—InEnTec(将固定床与等离子气化相结合)和Sierra Energy(改造传统高炉转炉进行垃圾气化)的技术。借鉴以往和当前的国际发展经验,凭借其庞大的气化规模和丰富的专业知识,逐步将垃圾作为原料融入到煤气化中可以作为中国迈向循环经济和无废城市的第一步。将垃圾与煤共气化技术作为通向垃圾气化的桥梁,不仅可以使中国利用现有的人力资本和基础设施,还可以创造新的就业和商业机会,并支持中国实现可持续的垃圾管理策略,即在进行焚烧和填埋之前先进行减量、再利用和再循环。然而,将煤气化技术直接用于垃圾气化具有挑战性并且充满了较大的风险。本研究分享了贝伦拉特和SVZ黑水泵在垃圾与煤共气化方面遇到的各种运行问题以及从中得到的最新认识。然后介绍了这些积累的经验及认识是如何被考虑并应用到德国弗莱贝格工业大学的能源化工所开发的Flexi技术当中,包括:基于BGL熔渣气化技术开发FlexiSlag固定床气化技术。基于GSP气化技术开发FlexiEntrained气流床气化技术。基于HTW气化技术开发FlexiCOORVED气化技术。目前,Flexi技术在德国弗莱贝格开展中试规模运行,其目标是实现:(1)多元原料气化(例如生物质、煤、石油焦、不同类型的废物);(2)100%垃圾气化;(3)灵活的目标产品(即调整配置得到最大化的合成气产出,其中可含有最大化或最小化的甲烷、焦油和油的含量)以支持全球向碳中和和无废城市转型。鉴于全球(包括中国)实现无废城市和碳中和的目标,垃圾气化技术的长远发展不仅要向100%垃圾气化发展,还必须满足以下可持续性标准才能做出实质性的贡献:(1)多元进料,能够灵活利用多种类型的(废物)原料。(2)碳回收率最大化,以确保垃圾中的碳转移到产品中,而不是在整个过程链中以CO_(2)的形式排放到环境中。(3)通过生产玻璃渣、从渣中回收金属和零废水排放,将环境影响降至最低。本研究最后根据上述三个可持续性标准对三种气化类型进行了定性的整体评价,阐述了它们在垃圾气化应用中的优势和劣势。 | LEE Roh Pin SEIDL Ludwig Georg HUANG Qiu-liang MEYER Bernd | 2021 | 燃料化学学报2021，49，8： | 10 |
| 6 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 7 | Influencing Factors on Rice Sheath Blight Epidemics in Integrated Rice-Duck System显示文摘Sheath blight, a disease caused by the fungus Rhizoctonia solani Kuhn (anamorph), has been the most economically significant disease of rice. It was frequently reported that the disease was well-controlled in integrated rice-duck system without the employment of fungicides. However, the effecting factors behind this phenomenon were rarely reported. In this research, experiment was carried out between two treatments, rice combined with ducks (RD) and conventional rice field without ducks rearing (CK) in early season rice paddy, to investigate the variations of sclerotia in floodwater and on rice plant, microclimate 10 cm above the waterline in rice paddy and activity of protective enzymes in rice plants. The results showed that the floating sclerotia in floodwater in RD was 86-91% lower than that in CK, and adhering sclerotia in rice plant in RD was 67-78% lower than that in CK. The relative humidity tested significantly lower and light intensity tested significantly higher in RD. The temperature in the early rice growth stages in RD was slightly lower than that in CK, but it was significantly higher (32.3-36.5°C) in the middle stage rice growth stages. The polyphenoloxidase (PPO) activity in RD were lower than that in CK, but the enhanced activity of phenylalanine ammonia-lyase (PAL), peroxidase (POD) and Chitinase was observed in different stages of rice growth in RD, especially the Chitinase which showed higher activity in all investigating days. | SU Pin LIAO Xiao-lan ZHANG Ya HUANG Huang | 2012 | Journal of Integrative Agriculture2012，11，9： | 6 |
| 8 | Impact of clinical research on public health policy of neonatal screening for congenital heart disease in China显示文摘Congenital heart disease(CHD)is one of the most common congenital malformations,with a prevalence of approximately 1%in live births.[1,2]Among them,1/4 to 1/3 are major CHD,including critical CHD(CCHD)and serious CHD,which require surgical or catheter intervention before 1 year of age.[3]Major CHD,if left untreated,may cause serious outcomes,such as heart failure,cardiogenic shock,acidosis,and hypoxic-ischemic brain damage,and is one of the leading causes of infant death and childhood disability.[4]The missed diagnosis rate of CCHD in four nurseries ranges from 13%to 48%in developed countries,[4,5]but in China,is>71%before serious symptoms occur after birth.[6]Therefore,screening for CHD is useful for early detection,diagnosis,and treatment and helps to improve prognosis and reduces the medical,economic,and mental burden to both the family and the society.[5,7] | Miao Yang Youping Tian Pin Jia Xiaojing Ma Xiaoling Ge Xiaojing Hu Quming Zhao Fang Liu Bing Jia Weili Yan Qing Gu Guoying Huang | 2022 | Chinese Medical Journal2022，，11： | 3 |
| 9 | γδT17 Cells Promote the Accumulation and Expansion of Myeloid-Derived Suppressor Cells in Human Colorectal Cancer显示文摘 | Pin Wu Dang Wu Chao Ni Jun Ye Wuzhen Chen Guoming Hu Zhen Wang Changrong Wang Zhigang Zhang Wenjie Xia Zhigang Chen Ke Wang Tao Zhang Jinghong Xu Yuehua Han Ting Zhang Xianguo Wu Jianwei Wang Weihua Gong Shu Zheng Fuming Qiu Jun Yan Jian Huang | 2014 | Immunity2014，，5： | 2 |
| 10 | Prophylactic Use of Transjugular Intrahepatic Portosystemic Shunt Aids in the Treatment of Refractory Ascites: Metaregression and Trial Sequential Meta-analysis显示文摘 | Ren Pin Chen Xiao Ju Zhu Ge Zhi Ming Huang Xiao Hua Ye Chang Yuan Hu Guang Rong Lu De Yi Lu Igor Longe Phemba | 2014 | Journal of Clinical Gastroenterology2014，，3： | 2 |
| 11 | Influencing factors on the preservation of lytic bacteriophage VP3显示文摘Long-term and stable preservation of bacteriophages is of crucial importance.Although many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is still little understanding,and a systematic description is lacking.In this study,we explored the influence of different factors on the preservation of lytic bacteriophage VP3,one of the typing bacteriophages of Vibrio cholerae O1 biotype El Tor,and attempted to optimize its preservation.We examined external factors,including temperature,solution,and cryoprotectant,in stable cooling/freezing conditions or alternate cooling/freezing and thawing.We found that whether in Luria-Bertani(LB)medium or SM buffer,in terms of 20-week stable cooling or freezing,−20℃ was the most damaging while 4℃,−80℃,and−196℃ were protective.Thirteen cycles of alternate cooling/freezing and thawing caused a loss in the survival rates of bacteriophages.The addition of cryoprotectant,glycerol(30%,w/v)or dimethyl sulfoxide(DMSO,10%,w/v)significantly improved the survival rates of bacteriophages preserved at−20℃.However,at 4℃,−80℃,and−196℃,the cryoprotectant effect was only slightly positive or even harmful.In summary,for bacteriophage VP3,the best preservation method is to directly preserve the bacteriophage stocks in LB medium at−80℃ or−196℃ instead of storing them in SM buffer or adding cryoprotectant.Our results provided insights into the external influencing factors on bacteriophage VP3 during preservation at low temperature and can be applied to the optimization of bacteriophage preservation in the future. | Yue Xiao Pin Huang Zhenzhou Huang Keyi Yu Yang Song Ning Guo Hang Dai Mengnan Jiang Yi Xu Duochun Wang Qiang Wei | 2022 | Biosafety and Health2022，4，5： | 2 |
| 12 | The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells显示文摘 | Yi-Hong Hsin Chun-Feng Chen Shing Huang Tung-Sheng Shih Ping-Shan Lai Pin Ju Chueh | 2008 | Toxicology Letters2008，，3： | 2 |
| 13 | Cell typespecific activation of P38 MAPK in the brain regions of hypoxic preconditioned mice显示文摘 | Bu Xiangning Huang Pin Qi Zhifeng | 2007 | Neurochem Int2007，51，： | 1 |
| 14 | The application of proton spectrometers at the SG-III facility for ICF implosion areal density diagnostics显示文摘Charged particle diagnostics is one of the required techniques for implosion areal density diagnostics at the SG-III facility.Several proton spectrometers are under development, and some preliminary areal density diagnostics have been carried out. The response of the key detector, CR39, to charged particles was investigated in detail. A new track profile simulation code based on a semi-empirical model was developed. The energy response of the CR39 detector was calibrated with the accelerator protons and alphas from a241 Am source. A proton spectrometer based on the filtered CR39 detector was developed, and D–D primary proton measurements were implemented. A step range filter spectrometer was developed,and preliminary areal density diagnostics was carried out. A wedged range filter spectrometer array made of Si with a higher resolution was designed and developed at the SG-III facility. A particle response simulation code by the Monte Carlo method and a spectra unfolding code were developed. The capability was evaluated in detail by simulations. | Xing Zhang Jianhua Zheng Ji Yan Zhenghua Yang Ming Su Yudong Pu Pin Yang Xufei Xie Li Chen Ming Chen Tianxuan Huang Shao'en Jiang Shenye Liu Jiamin Yang | 2015 | High Power Laser Science and Engineering2015，3，4： | 1 |
| 15 | Accuracy improvement of wireEDM by real-time wire tension control显示文摘 | Yan Mu Tian Huang Pin Hsum | 2004 | International Journal of Machine Tools2004，2004，44： | 1 |
| 16 | Technology Forecasting Via Published Patent Applications and Patent Grants 显示文摘 | Dar- Zcn Chem Chang - Pin Lin Mu - Hsuan Huang ctal | 2012 | Journal of Marine Science and Technology2012，20，4： | 1 |
| 17 | ACE2 expression is regulated by AhR in SARS-CoV-2-infected macaques显示文摘During the current outbreak of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),more than 115 million people have been infected,and 2.5 million have died.1,2 Despite such great harm to human health,the pathogenesis of COVID-19 remains unclear.As the first step in the pathogenetic process,viral entry is mediated by the binding of the SARS-CoV-2 surface spike(S)protein to angiotensin-converting enzyme 2(ACE2)on host cells,such as lung epithelial cells.As an alternative to S protein-blocking strategies,manipulating host cell ACE2 expression may exert an inhibitory effect on SARS-CoV-2 infection.However,the molecular mechanism regulating ACE2 expression remains unclear. | Jiadi Lv Pin Yu Zhenfeng Wang Wei Deng Linlin Bao Jiangning Liu Fengli Li Qiangqiang Zhu Nannan Zhou Qi Lv Guanpeng Wang Shunyi Wang Yabo Zhou Jiangping Song Wei-Min Tong Yuying Liu Chuan Qin Bo Huang | 2021 | Cellular & Molecular Immunology2021，18，5： | 1 |
| 18 | Measuring competitive ad- vantage with an asset - light valuation model 显示文摘 | Chiun - Sin Lin Chih - Pin Huang | 2011 | Afxican Journal of Business Management2011，，13： | 1 |
| 19 | Kinetics study on the formation of resol with high content of hydroxymethyl group 显示文摘 | Huang G S Pin G Zhang Y F | 2008 | J Appl Polym Sci2008，107，5： | 1 |
| 20 | Cell typespecific activation of p38 MAPK in the brain regions of hypoxie preconditioned mice 显示文摘 | Bu Xiangning Huang Pin Qi Zhifeng etal | 2007 | Neurochem Int2007，51，： | 1 |
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