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| 1 | The first offshore natural gas hydrate production test in South China Sea显示文摘Natural gas hydrates (NGH)is one of key future clean energy resources.Its industrialized development will help remit the huge demand of global natural gas,relieve the increasing pressure of the environment, and play a vital role in the green sustainable growth of human societies.Based on nearly two decades' studying on the reservoir characteristics in the South China Sea (SCS)and the knowledge of reservoir system,the China Geological Survey (CGS)conducted the first production test on an optimal target selected in Shenhu area SCS in 2017.Guided by the 'three-phase control'exploitation theory which focused on formation stabilization,technologies such as formation fluid extraction,well drilling and completing, reservoir stimulating,sand controlling,environmental monitoring,monitoring and preventing of secondary formation of hydrates were applied.The test lasted for 60 days from May 10^th when starting to pump, drop pressure and ignite to well killing on July 9^th,with gas production of 3.09×10^5 m^3 in total,which is a world record with the longest continuous duration of gas production and maximal gas yield.This successful test brings a significant breakthrough on safety control of NGH production. | Jin-fa Li Jian-liang Ye Xu-wen Qin Hai-jun Qiu Neng-you Wu Hai-long Lu Wen-wei Xie Jing-an Lu Fei Peng Zhen-qiang Xu Cheng Lu Zeng-gui Kuang Jian-gong Wei Qian-yong Liang Hong-feng Lu Bei-bei Kou | 2018 | China Geology2018，1，1： | 117 |
| 2 | DK crush technique: modified treatment of bifurcation lesions in coronary artery显示文摘 | CHEN Shao-liang YE Fei ZHANG Jun-jie ZHU Zhong-sheng LIN Song SHAN Shou-jie LIU Zhi-zhong LIU Yan DUAN Bao-xiang GE Jun-bo | 2005 | Chinese Medical Journal2005，，20： | 35 |
| 3 | A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy显示文摘anti-CTLA-4 抗体由阻止 B7-CTLA-4 相互作用接近否定发信号引起肿瘤拒绝,这被假定。在比临床上有效的 dosing 完成的血浆层次更加高的集中,令人惊讶地, anti-CTLA-4 抗体 Ipilimumab 不由 CTLA-4 也不 CTLA-4 绑定堵住任何一 B7 trans-endocytosis 到使不能调动或联系房间的 B7。因而, Ipilimumab 不从人性化的任何一个 CTLA4 基因在树枝状的房间(DC ) 上增加 B7 (Ctla4 h/h ) 或人的 CD34 + 茎重新组成房间的 NSG 老鼠。在 Ctla4 高效地表示人和老鼠 CTLA4 基因,绑在人的 anti-CTLA-4 抗体然而并非老鼠 CTLA-4 的 h/m 老鼠导致 Treg 弄空和 Fc 受体依赖者肿瘤拒绝。堵住的抗体 L3D10 比得上在引起肿瘤拒绝的非阻塞的 Ipilimumab。显著地,输在人类化期间堵住活动的 L3D10 子孙在导致 Treg 弄空和肿瘤拒绝仍然保持充分能干。有效地在淋巴的器官堵住 CD4 T 细胞激活和 de novo CD8 T 细胞 priming 的 Anti-B7 抗体否定地不影响 Ipilimumab 的 immunotherapeutic 效果。因此,临床上有效的 anti-CTLA-4 mAb 由独立于检查点封锁的机制引起肿瘤拒绝但是依赖于主人 Fc 受体。我们为 CTLA-4 检查点的一个重评价的数据电话封锁假设并且为安全、有效的 anti-CTLA-4 mAbs 的下一代提供新卓见。 | Xuexiang Du Fei Tang Mingyue Liu Juanjuan Su Yan Zhang Wei Wu Martin Devenport Christopher A Lazarski Peng Zhang Xu Wang Peiying Ye Changyu Wang Eugene Hwang Tinghui Zhu Ting Xu Pan Zheng Yang Liu | 2018 | Cell Research2018，28，4： | 25 |
| 4 | Clinical practice guidelines for sentinel lymph node biopsy in patients with early-stage breast cancer: Chinese Society of Breast Surgery (CSBrS) practice guidelines 2021显示文摘Axillary lymph node assessment is one of the important indicators in the clinical pathological staging of breast cancer.Studies have shown that sentinel lymph node biopsy(SLNB)has the advantages of fewer complications and less trauma than conventional axillary lymph node dissection(ALND)/[1] Common mapping methods for SLNB include blue dye,radioisotopes,a combination of blue dye and radioisotopes,and fluorescence imaging. | Jing-Ming Ye Bao-Liang Guo Qian Liu Fei Ma Hong-Jin Liu Qian Wu Ling Xin Yuan-Jia Cheng Hong Zhang Shuang Zhang Xue-Ning Duan Jian-Guo Zhang Yin-Hua Liu | 2021 | Chinese Medical Journal2021，，8： | 26 |
| 5 | 电针和经皮穴位电刺激技术在生殖医学中的应用:专家共识(英文)显示文摘本文从传统针刺治疗不孕症的选穴依据与现代电针/经皮穴位电刺激技术的相关原理着手,从最优刺激参数选择、穴位辨证、疗程与治疗次数的确定及基本原理等方面阐述了电针/经皮穴位电刺激技术在多囊卵巢综合症、取卵镇痛、卵巢储备功能降低、胚胎移植以及男性少/弱精症等生殖领域中的具体应用。首次对电针/经皮穴位电刺激技术在生殖领域优势病种中的应用进行了系统的分析和总结,在最优刺激参数选择、穴位辨证、疗程与治疗次数的确定等方面提出了一系列专家共识,并为临床实践提供了指导意见和理论依据。 | Fan QU Rong LI Wei SUN Ge LIN Rong ZHANG Jing YANG Li TIAN Guo-gang XING Hui JIANG Fei GONG Xiao-yan LIANG Yan MENG Jia-yin LIU Li-ying ZHOU Shu-yu WANG Yan WU Yi-jing HE Jia-yu YE Song-ping HAN Ji-sheng HAN | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 24 |
| 6 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 7 | Therapeutic effect of Zijin capsule in liver fibrosis in rats显示文摘TherapeuticefectofZijincapsuleinliverfibrosisinratsCAIDaYongZHAOGang,CHENJiaChun,YEGanMei,BINGFeiHongandFANBuWuSubjecth... | CAI Da Yong, ZHAO Gang, CHEN Jia Chun, YE Gan Mei, BING Fei Hong and FAN Bu Wu | 1998 | World Journal of Gastroenterology1998，4，3： | 20 |
| 8 | Individual variability in the disposition of and response to clopidogrel. Pharmacogenomics and beyond显示文摘 | Xie, Hong-Guang | 2011 | 南京医科大学学报(自然科学版)2011，31，6： | 20 |
| 9 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 10 | High-intensity focused ultrasound treatment for patients with unresectable pancreatic cancer显示文摘BACKGROUND: High-intensity focused ultrasound (HIFU) is a non-invasive method of solid tissue ablation therapy. However, only a few studies have reported the effect of HIFU for unresectable pancreatic cancer. This study aimed to evaluate the clinical benefits, survival time and complications associated with the use of HIFU ablation in patients with unresectable pancreatic cancer. METHODS: Twenty-five patients with unresectable pancreatic cancer were enrolled in our study. All patients received HIFU therapy for tumors at least once. The therapeutic effects of HIFU was evaluated in terms of Karnofsky performance status (KPS) scores, pain relief, serum CA19-9, and imaging by B-US and CT before and after the therapy. We also recorded median overall survival time and complications caused by the treatment. RESULTS: In the 25 patients, KPS scores were above 60, and increased KPS was observed in 23 patients after treatment. Pain relief occurred in 23 patients. Serum CA19-9 levels were significantly reduced one month after HIFU treatment and became negative in 5 patients. B-US revealed enhanced tumor echogenicity in 13 patients and decreased tumor blood supply in 9. Tumor necrosis was confirmed by CT in 8 patients one month after HIFU treatment. The median overall survival time was 10 months, and the 1-year survival rate was 42%. No severe complications were observed after HIFU treatment. CONCLUSION: HIFU can effectively relieve pain, increase KPS, decrease tumor growth and prolong the survival time of patients with unresectable pancreatic cancer. | Peng-Zhou Li, Shai-Hong Zhu, Wei He, Li-Yong Zhu, Sheng-Ping Liu, Yan Liu, Guo-Hui Wang, Fei Ye Department of General Surgery ,Department of Radiology, Third Xiangya Hospital, Central South University, Changsha 410013, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，6： | 19 |
| 11 | Antibacterial Effect of Cinnamon Oil Combined with Thyme or Clove Oil显示文摘Antibacterial effect of cinnamon oil combined with thyme or clove oil was studied in this paper.Agar dilution method was used to determine the minimum inhibitory concentrations(MICs) of the essential oils(EOs) of cinnamon,thyme,and clove oil against three Gram-positive bacteria(Bacillus subtilis,Bacillus cereus,and Staphylococcus aureus),and two Gram-negative bacteria(Escherichia coli and Salmonella typhimurium).The results showed that cinnamon was a promising antibacterial substance with MIC ranged from 0.1 to 0.4 μL mL-1 for the five bacterial species.Agar dilution checkerboard method was used to test the combined antibacterial effect of cinnamon oil with thyme or clove oil.Combination of cinnamon and thyme oil showed an additive effect against all selected bacteria,and combination of cinnamon and clove oil displayed an additive effect against B.subtilis,B.cereus,S.aureus,and an indifferent effect against E.coli and S.typhimurium.Furthermore,gas chromatography-mass spectrometry(GC-MS) measurement was used to analyze the components of the EOs,and the main components of cinnamon,thyme,and clove were cinnamaldehyde,thymol,carvacrol and p-cymene,and eugenol,respectively. | LU Fei DING Yi-cheng YE Xing-qian DING Yu-ting | 2011 | Agricultural Sciences in China2011，10，9： | 19 |
| 12 | Capping CsPbBr3 with ZnO to improve performance and stability of perovskite memristors显示文摘技术与快信息存储为设备导致了迫切需要并且处理的信息的快速的发展,高密度,和低精力消费。Memristors 被认为是有所有上述的优点的下一代的记忆设备。最近,有机金属的卤化物 perovskites 被报导为 memristors 正在答应活跃材料,尽管他们有差的稳定性和低劣性能。此处,我们第一次基于无机的卤化物 perovskite (CsPbBr 3, CPB ) 报导稳定、高效的 memristors 的制造。设备有电导致地双极的抵抗切换(物件)并且有大开/关比率的存储器行为(> 10 5),低工作电压(< 1 V )并且精力消费,长数据保留(> 10 4 s ),并且高环境的稳定性,它经由在设备以内盖住的 ZnO 被完成。如此的一个图案能被使适应各种各样的设备。另外,在 CPB 和 ZnO 之间的异质接面以快速的反应速度赋予设备以超过 10 3 的导致光的物件效果(< 1 ms ) ,它使我们能由同时改变光和电场调节电阻状态。信息存储和处理能力的联合完成的如此的多功能的设备为超越传统的体系结构的未来计算有潜在的应用程序。 | Ye Wu Yi Wei Yong Huang Fei Cao Dejian Yu Xiaoming Li Haibo Zeng | 2017 | Nano Research2017，10，5： | 17 |
| 13 | Intra-herb pharmacokinetics interaction between quercetin and isorhamentin显示文摘瞄准:橡黄素和 isorhamnetin 是一些植物摘录的普通成分,例如银杏叶子的摘录和 Hippophae rhamnoides L 的全部的黄酮。在 isorhamnetin 和橡黄素之间的 intra 植物 pharmacokinetics 相互作用在现在的学习被调查。方法:人的 MDR1 cDNA transfected MDCKII 房间被用来验证 isorhamnein 是否与 P-gp 交往了。Caco-2 运输试金并且一使随机化,在老鼠的 3 方法转线路 pharmacokinetics 学习被用来调查 pharmacokinetics 相互作用。HPLC 被用来决定房间运输样品。橡黄素和 isorhamnetin 的全部的血浆集中被处理被液体层析双人脚踏车团 spectrometry (LC-MS/MS ) 与 β-glucuronidase 和 sulfatase 决定。结果:越过人的 MDR1 cDNA transfected MDCKII 房间, Caco-2 房间和野类型的 MDCKII 房间的 isorhamnetin 的渗透比率(吸收性的 permeability/secretive 渗透) 分别地是 0.25 ± 0 .02, 0.74 ± 0 .05,和 1.41 ± 0 .06。这结果在 isorhamnetin 的房间流出证明了 P-gp 的角色。当越过 Caco-2 房间单层与对方一起共同搬运时, isorhamnetin 和橡黄素的渗透比率到 4.3 和 2.2 次增加了。在到老鼠的与对方一起的 coadministration 以后, C 72 h , 和 isorhamnetin 和橡黄素的 AUC 0 ∞
显著地与单个管理相比增加了的最大 , AUC 0。结论:上述结果证明了 intra 植物是在橡黄素和 isorhamentin 之间的 pharmacokinetics 相互作用。而另外的药流出抽, P-gp 可能起一个重要作用,例如多药抵抗伙伴蛋白质 2 并且乳癌抵抗蛋白质,可能被包含。除药植物相互作用以外,因此, intra 植物相互作用可能与草药底的疗法的宽使用被带进看法。 | Ke LAN Jian-lin HE Yang TIAN Fei TAN Xue-hua JIANG Ling WANG Li-ming YE | 2008 | Acta Pharmacologica Sinica2008，29，11： | 16 |
| 14 | AKT-modified autologous intracoronary mesenchymal stem cells prevent remodeling and repair in swine infarcted myocardium显示文摘成年骨头的背景移植导出髓的间充质的干细胞(MSC ) 为心脏的修理作为策略被建议了后面的心肌的损坏。然而,代替失去的心肌层的房间移植策略被无能限制交付抵抗 peritransplantation 接枝房间死亡的大量房间。因此,我们开始孤立并且膨胀成年的猪骨头,并且设计这些房间到 overexpress AKT1 (蛋白质 kinase B )导出髓的 MSC ,为了测试假设,那 AKT1 设计了 MSC 对 apoptosis 更抵抗并且能在移植以后提高心脏的修理进 ischemic 猪 heart.Methods CDS ( AKT1 的规定领域) AKT1-cDNA 碎片被放大,并且 MSC 是有 pCDH1-AKT1 shuttling plasmid 的 transfected 追随者合成。西方的弄污分析和即时反向的抄写聚合酶链反应(RT-PCR ) 被执行。心肌的梗塞(Ml ) 模型在 Meishan 猪被构造,并且心脏的功能被磁性的回声成像(MRI ) 评估大小和 4 个星期以后的 echocardiography。所有猪被分到四个组:控制(A) , DMEM (B) , MSC (C) ,和 AKT-transfected (D) 。MSC 是有 AKT1 基因,和自体同源的标记 BrdU 的干细胞的 transfected | YU Yun-sheng SHEN Zhen-ya YE Wen-xue HUANG Hao-yue HUA Fei CHEN Yi-huan CHEN Ke LAO Wei-jie TAO Li | 2010 | Chinese Medical Journal2010，，13： | 15 |
| 15 | The application of geostatistics in grain size trend analysis: A case study of eastern Beibu Gulf显示文摘有 71 件表面沉积样品,从东方 Beibu 海湾收集。片刻参数(即吝啬的尺寸,排序并且偏斜度) 在使用谷物尺寸分析以后被获得。geostatistical 分析然后被使用为这些参数学习空间自相关;当时范围,在 semivariogram 的一个参数米空间自相关的规模,被估计。结果显示为排序系数的范围身体上是有意义的。从谷物尺寸趋势分析模型计算的趋势向量根据以前的研究与年度海洋发行量模式和沉积运输率一致。因此,从吝啬的尺寸的 semivariogram 导出的范围能在谷物尺寸趋势分析被用作典型距离,它可以移开基于经验或严峻的方法得到典型距离的传统的方法引起的偏爱。因此从分析能也在未来为沉积采样密度的决心为有用信息提供的 geostatistical 的结果回答工作。 | MA Fei WANG Yaping LI Yan YE Changjiang XU Zhiwei ZHANG Fan | 2010 | Journal of Geographical Sciences2010，20，1： | 14 |
| 16 | Antioxidant and immunomodulatory activities of a polysaccharide from Flammulina velutipes显示文摘OBJECTIVE: To evaluate the antioxidant and immunomodulatory activities of a unique polysaccharide from the medicinal fungus Flammulina velutipes in vitro.METHODS: Using water extraction and alcohol precipitation, crude polysaccharides were obtained. After purification by DEAE-cellulose 52 ion exchange chromatography and Sephacryl S-300 HR gel filtration chromatography, High performance liquid chromatography equipped with evaporative light-scattering detector, Infrared radiation and Nuclear magnetic resonance were used to evaluate the structure of the polysaccharide. Its immunomodulatory activity was measured by examining the production of nitric oxide(NO) and cytokine secretion, and via lymphocyte proliferation experiments. Its effects on the scavenging activities of hydroxyl radical, superoxide anion and reducing power were also measured.RESULTS: A water-soluble polysaccharide, Flammulina velutipes polysaccharide I-A(FVP I-A), was obtained with a molecular mass of 8.14×104Da determined by high performance gel permeation chromatography. An in vitro antioxidant assay indicated that FVP I-A could scavenge hydroxyl radical, superoxide anion and possessed reducing power and could largely promote NO production and augment the interleukin-1β, interleukin-6, and tumor necrosis factor-α secretion by RAW264.7 macrophages(P<0.05). Moreover, FVP I-A could promote lymphocyte proliferation(P<0.05), and synergistically enhance the augmentation of the proliferation of mouse lymphocytes by concanavalin A and lipopolysaccharides(P<0.01, P<0.05).CONCLUSION: The FVP I-A obtained from Flammulina velutipes possessed antioxidant activity and could enhance non-specific and specific immune responses in vitro. | Ming Wu Xia Luo Xiaoyan Xu Wei Wei Mengyao Yu Nan Jiang Liming Ye Zhirong Yang Xiaofan Fei | 2014 | Journal of Traditional Chinese Medicine2014，34，6： | 14 |
| 17 | Current achievements and future prospects in the genetic breeding of chrysanthemum:a review显示文摘Chrysanthemum(Chrysanthemum morifolium Ramat.)is a leading flower with applied value worldwide.Developing new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes,plant architectures,flowering times,postharvest quality,and biotic and abiotic stress tolerance in a time-and cost-efficient manner is the ultimate goal for breeders.Various breeding strategies have been employed to improve the aforementioned traits,ranging from conventional techniques,including crossbreeding and mutation breeding,to a series of molecular breeding methods,including transgenic technology,genome editing,and marker-assisted selection(MAS).In addition,the recent extensive advances in high-throughput technologies,especially genomics,transcriptomics,proteomics,metabolomics,and microbiomics,which are collectively referred to as omics platforms,have led to the collection of substantial amounts of data.Integration of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important traits.Several attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of chrysanthemum.However,applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge,primarily due to the high heterozygosity and polyploidy of the species.This review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum. | Jiangshuo Su Jiafu Jiang Fei Zhang Ye Liu Lian Ding Sumei Chen Fadi Chen | 2019 | Horticulture Research2019，6，1： | 13 |
| 18 | Uncarboxylated osteocalcin ameliorates hepatic glucose and lipid metabolism in KKAy mice via activating insulin signaling pathway显示文摘Osteocalcin,expressed in osteoblasts of the bone marrow,undergoes post-translational carboxylation and deposits in mineralized bone matrix.A portion of osteocalcin remains uncarboxylated(uncarboxylated osteocalcin,GluOC)that is released into blood where it functions as a hormone to regulate insulin secretion and insulin sensitivity.As insulin resistance is closely associated with metabolic syndrome,this study is aimed to elucidate how GluOC regulates glucose and lipid metabolism in KKAy mice,an animal model displaying obese,hyperglycemia,hyperinsulinemia,insulin resistance,and hepatic steatosis.GluOC(3,30 ng/g per day,ig)was orally administered to female KKAy mice for 4 weeks.Whole-body insulin sensitivity,glucose metabolism,hepatic steatosis,dyslipidemia were examined using routine laboratory assays.We found that GluOC administration significantly enhanced insulin sensitivity in KKAy mice by activating hepatic IRβ/PI3K/Akt pathway and elevated the whole-body insulin sensitivity with decreased FPI and HOMA-IR index.Furthermore,GluOC administration alleviated hyperglycemia through suppressing gluconeogenesis and promoting glycogen synthesis in KKAy mice and in cultured hepatocytes in vitro.Moreover,GluOC administration dose-dependently ameliorated dyslipidemia and attenuated hepatic steatosis in KKAy mice by inhibiting hepatic de novo lipogenesis and promoting fatty-acidβ-oxidation.These results demonstrate that GluOC effectively enhances hepatic insulin sensitivity,improves hyperglycemia and ameliorates hepatic steatosis in KKAy mice,suggesting that GluOC could be a promising drug candidate for treating metabolic syndrome. | Xiao-lin Zhang Ya-nan Wang Lu-yao Ma Zhong-sheng Liu Fei Ye Jian-hong Yang | 2020 | Acta Pharmacologica Sinica2020，41，3： | 13 |
| 19 | Chinese physicians' perceptions of fecal microbiota transplantation显示文摘AIM: To explore Chinese physicians' perceptions towards fecal microbiota transplantation(FMT) and to provide information and an assessment of FMT development in China.METHODS: A self-administered questionnaire was developed according to the FMT practice guidelines and was distributed to physicians in hospitals via Internet Research Electronic Data Capture(REDcap) software and electronic mails to assess their attitudes toward and knowledge of FMT. The questionnaire included a brief introduction of FMT that was followed by 20 questions. The participants were required to respond voluntarily, under the condition of anonymity and without compensation. Except for the fill-in-the-blank questions, all of the other questions were required in the REDcap data collection systems, and the emailed questionnaires were completed based on eligibility.RESULTS: Up to December 9, 2014, 844 eligible questionnaires were received out of the 980 distributed questionnaires, with a response rate of 86.1%. Among the participants, 87.3% were from tertiary hospitals, and there were 647(76.7%) gastroenterologists and 197(23.3%) physicians in other departments(nongastroenterologists). Gastroenterologists' awareness of FMT prior to the survey was much higher than non-gastroenterologists'(54.3 vs 16.5%, P < 0.001); however, acceptance of FMT was not statistically different(92.4 vs 87.1%, P = 0.1603). Major concerns of FMT included the following: acceptability to patients(79.2%), absence of guidelines(56.9%), and administration and ethics(46.5%). On the basis of understanding, the FMT indications preferred byphysicians were recurrent Clostridium difficile infection(86.7%), inflammatory bowel disease combined with Clostridium difficile infection(78.6%), refractory ulcerative colitis(70.9%), ulcerative colitis(65.4%), Crohn's disease(59.4%), chronic constipation(43.7%), irritable bowel syndrome(39.1%), obesity(28.1%) and type 2 diabetes(23.9%). For donor selection, the majority of physicians preferred individuals with a similar gut flora environment to the recipients. 76.6% of physicians chose lower gastrointestinal tract as the administration approach. 69.2% of physicians considered FMT a safe treatment. CONCLUSION: Chinese physicians have awareness and a high acceptance of FMT, especially gastroenterologists, which provides the grounds and conditions for the development of this novel treatment in China. Physicians' greatest concerns were patient acceptability and absence of guidelines. | Rong-Rong Ren Gang Sun Yun-Sheng Yang Li-Hua Peng Shu-Fang Wang Xiao-Hong Shi Jing-Quan Zhao Yong-Ling Ban Fei Pan Xue-Hong Wang Wei Lu Jian-Lin Ren Ying Song Jiang-Bin Wang Qi-Ming Lu Wen-Yuan Bai Xiao-Ping Wu Zi-Kai Wang Xiao-Mei Zhang Ye Chen | 2016 | World Journal of Gastroenterology2016，22，19： | 12 |
| 20 | Suppression of esophageal cancer cell growth using curcumin,(-)-epigallocatechin-3-gallate and lovastatin显示文摘AIM:To determine the effects of curcumin,(-)-epigallocatechin-3-gallate (EGCG),lovastatin,and their combinations on inhibition of esophageal cancer.METHODS:Esophageal cancer TE-8 and SKGT-4 cell lines were subjected to cell viability methyl thiazolyl tetrazolium and tumor cell invasion assays in vitro and tumor formation and growth in nude mouse xenografts with or without curcumin,EGCG and lovastatin treatment.Gene expression was detected using immunohistochemistry and Western blotting in tumor cell lines,tumor xenografts and human esophageal cancer tissues,respectively.RESULTS:These drugs individually or in combinations significantly reduced the viability and invasion capacity of esophageal cancer cells in vitro.Molecularly,these three agents reduced the expression of phosphorylated extracellular-signal-regulated kinases (Erk1/2),c-Jun and cyclooxygenase-2 (COX-2),but activated caspase 3 in esophageal cancer cells.The nude mouse xenograft assay showed that EGCG and the combinations of curcumin,EGCG and lovastatin suppressed esophageal cancer cell growth and reduced the expression of Ki67,phosphorylated Erk1/2 and COX-2.The expression of phosphorylated Erk1/2 and COX-2 in esophageal cancer tissue specimens was also analyzed using immunohistochemistry.The data demonstrated that 77 of 156 (49.4%) tumors expressed phosphorylated Erk1/2 and that 121 of 156 (77.6%) esophageal cancers expressed COX-2 protein.In particular,phosphorylated Erk1/2 was expressed in 23 of 50 (46%) cases of esophageal squamous cell carcinoma (SCC) and in 54 of 106 (50.9%) cases of adenocarcinoma,while COX-2 was expressed in 39 of 50 (78%) esophageal SCC and in 82 of 106 (77.4%) esophageal adenocarcinoma.CONCLUSION:The combinations of curcumin,EGCG and lovastatin were able to suppress esophageal cancer cell growth in vitro and in nude mouse xenografts,these drugs also inhibited phosphorylated Erk1/2,c-Jun and COX-2 expression. | Fei Ye Gui-Hong Zhang Bao-Xiang Guan Xiao-Chun Xu | 2012 | World Journal of Gastroenterology2012，18，2： | 12 |
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