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| 1 | Aberrant glycosylation of the anti-Thomsen-Friedenreich glycotope immunoglobulin G in gastric cancer patients显示文摘AIM: To study whether alterations in the glycosylation of immunoglobulin G (IgG) specific to the ThomsenFriedenreich glycotope (TF) have diagnostic and prognostic potential in gastric cancer. METHODS: Serum samples were obtained from patients with histologically verified gastric carcinoma (n = 89), healthy blood donors (n = 40), and patients with benign stomach diseases (n = 22). The lectin-enzymelinked immunosorbent assay-based glycoprofiling of TF-specific IgG (anti-TF IgG) was performed using synthetic TF-polyacrylamide conjugate as antigen, total IgG purified by affinity chromatography on protein G sepharose, and lectins of various sugar specificities: mannose-specific concanavalin A (ConA), fucose-specificAleuria aurantia lectin (AAL) and sialic acid-specific Sambucus nigra agglutinin (SNA). The sensitivity and specificity of the differences between cancer patients and controls were evaluated by receiver operator characteristic (ROC) curve analysis. Overall survival was analyzed by the Kaplan-Meier method. Time-dependent ROC curve statistics were applied to determine cut-off values for survival analysis. All calculations and comparisons were performed using the GraphPad Prism 5 and SPSS 15.0 software.RESULTS: The level of TF-specific IgG was significantly increased in cancer patients compared with non-cancer controls (P < 0.001). This increase was pronounced mostly in stage 1 of the disease. Cancer patients showed a higher level of ConA binding to antiTF-IgG (P < 0.05) and a very low level of SNA lectin binding (P = 0.0001). No appreciable stage-dependency of the binding of any lectin to anti-TF IgG was found. A strong positive correlation between the binding of AAL and SNA was found in all groups studied (r = 0.71-0.72; P < 0.0001). The changes in ConA reactivity were not related to those of the fucoseor sialic acid-specific lectin. Changes in the SNA binding index and the ConA/SNA binding ratio demonstrated good sensitivity and specificity for stomach cancer: sensitivity 78.79% (95%CI: 61.09-91.02) and 72.73% (95%CI: 57.21-85.04); specificity 79.17 (95%CI: 65.01-89.53) and 88.64% (95%CI: 71.8-96.6), for the SNA binding index and the ConA/SNA binding ratio, respectively. The other combinations of lectins did not improve the accuracy of the assay. The low level of ConA-positive anti-TF IgG was associated with a survival benefit in cancer patients (HR = 1.56; 95%CI: 0.78-3.09; P = 0.19), especially in stages 3-4 of the disease (HR = 2.17; 95%CI: 0.98-4.79; P = 0.048). A significantly better survival rate was found in all cancer patients with a low reactivity of anti-TF IgG to the fucose-specific AAL lectin (HR = 2.39; 95%CI: 1.0-5.7;P = 0.038).CONCLUSION: The changes in the TF-specific IgG glycosylation pattern can be used as a biomarker for stomach cancer detection, and to predict patient survival. | Kristel Kodar Jelena Izotova Kersti Klaamas Boris Sergeyev Lilian Jrvekülg Oleg Kurtenkov | 2013 | World Journal of Gastroenterology2013，19，23： | 3 |
| 2 | Evaluation of prenatal risk factors for prediction of outcome in right heart lesions: CVP Score in fetal right heart defects显示文摘 | Ana Luisa Neves Leigh Mathias Marilyn Wilhm Jennifer Leshko Kersti K. Linask Tiago Henriques-Coelho José C. Areias James C. Huhta | 2014 | The Journal of Maternal-Fetal & Neonatal Medicine2014，，14： | 2 |
| 3 | Comparison of knowledge, attitudes and behaviour regarding smoking among Estonian and Finnish physicians显示文摘 | Kersti P?rna Kaja Rahu No?l C. Barengo Mati Rahu Patrick H. Sandstr?m Vesa J. Jormanainen Markku T. Myllykangas | 2005 | Sozial - und Pr?ventivmedizin SPM2005，，6： | 1 |
| 4 | Ecological risk assessment of the deliberate release of genetically modified microorganisms显示文摘 | KERSTI GUST JANET K JANSSON | 1993 | A MBIO1993，22，4： | 1 |
| 5 | Geographies of food: ’Afters’显示文摘 | Cook Ian Hobson Kersty Hallett Lucius Guthman Julie Murphy Andrew Hulme Alison Sheller Mimi Crewe Louise Nally David Roe Emma Mather Charles Kingsbury Paul Slocum Rachel Imai Shoko Duruz Jean Philo Chris Buller Henry Goodman Mike Hayes-C | 2011 | Progress in Human Geography2011，，1： | 1 |
| 6 | Long-term chan ges and seasonal development of phytoplankton in a strongly stratified hypertrophic lake显示文摘 | Kersti K Reet L Peeter N Ingmar O | 2005 | Hydrobiologia2005，547，： | 1 |
| 7 | Comparison of fertilization, cleavage and pregnancy rates of oo- cytes from large and small follicles显示文摘 | Christina Bergh Helena Broden Kersti Lundin and Lars Hamberg- er | 1998 | Human Reproduction1998，13，7： | 1 |
| 8 | Differences in human papillomavirus type distribution in high‐grade cervical intraepithelial neoplasia and invasive cervical cancer in Europe显示文摘 | Wiebren A. Tjalma Alison Fiander Olaf Reich Ned Powell Andrzej M. Nowakowski Benny Kirschner Robert Koiss John O’Leary Elmar A. Joura Mats Rosenlund Brigitte Colau Doris Schledermann Kersti Kukk Vasileia Damaskou Maria Repanti Radu Vladareanu Larisa Kolom | 2012 | Int J Cancer2012，，4： | 1 |
| 9 | Long-term changes and seasonal development of phytoplankton in a strongly stratified hypertrophic lake显示文摘 | Kersti K Reet L Peeter N | 2005 | Hydrobiologia2005，547，： | 1 |
| 10 | A new method for the N-deacetylation of carbohydrates显示文摘 | Christina Erbing Kersti Granath Lennart Kene | 1976 | Carbohydrate Research1976，47，2： | 1 |
| 11 | Comparison of Socioeconomic Differences in Self-Perceived Health in Estonia and Finland显示文摘 | Kersti Pamalnge Ringmets | 2010 | Scandinavian Journal of Public Health2010，，3： | 1 |
| 12 | Long-term changes and seasonal development of phytoplankton in a strongly stratified hypertrophic lake 显示文摘 | Kersti K Reet L Peeter N Ingmar O | 2005 | Hydrobiologia2005，547，： | 1 |
| 13 | Cell guidance by magnetic nanowires显示文摘 | Fredrik Johansson Malin Jonssen Kersti Alm | 2010 | Experimental Cell research2010，316，： | 1 |
| 14 | Immunoglobulin G Fc N-glycan profiling in patients with gastric cancer by LC-ESI-MS: relation to tumor progression and survival显示文摘 | Kristel Kodar Johannes Stadlmann Kersti Klaamas Boris Sergeyev Oleg Kurtenkov | 2012 | Glycoconjugate Journal2012，，1： | 1 |
| 15 | Long-term changes and seasonal development of phytoplankton in a strongly stratified hypertrophic lake显示文摘 | Kersti K Reet L Peeter N | 2005 | Hydro-Biologia2005，547，： | 1 |
| 16 | Influence of ovarian stimulation with HP hMG or recombinant FSH on embryo quality parameters in patients undergoing IVF 显示文摘 | Soren Z Kersti L Janssens R | 2007 | Hum Reprod2007，22，9： | 1 |
| 17 | Influence of ovar- ian stimulation with HP - hMG or recombinant FSH on embryo quality parameters in patients undergoing IVF 显示文摘 | Soren Ziebe Kersti Lundin Ronny Janssens | 2007 | Human Reproduction2007，22，9： | 1 |
| 18 | Extraction of genomic DNA from yeasts for PCR-based applications显示文摘 | Marko L Kersti K Arnold K | 2011 | Biotechniques2011，50，5： | 1 |
| 19 | Influence of ovarian stim- ulation with HP-hMG or recombinant FSH on embryo quality pa- rameters in patients undergoing IVF显示文摘 | Soren Ziebe Kersti L Janssens R | 2007 | Hum Reprod2007，22，9： | 1 |
| 20 | Long-term changes and seasonal development of phyto-plankton in a strongly stratified hypertrophic lake 显示文摘 | Kersti K Reet L Peeter N ea al | 2005 | Hydrobiologia2005，547，： | 1 |
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