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| 1 | Aberrant glycosylation of the anti-Thomsen-Friedenreich glycotope immunoglobulin G in gastric cancer patients显示文摘AIM: To study whether alterations in the glycosylation of immunoglobulin G (IgG) specific to the ThomsenFriedenreich glycotope (TF) have diagnostic and prognostic potential in gastric cancer. METHODS: Serum samples were obtained from patients with histologically verified gastric carcinoma (n = 89), healthy blood donors (n = 40), and patients with benign stomach diseases (n = 22). The lectin-enzymelinked immunosorbent assay-based glycoprofiling of TF-specific IgG (anti-TF IgG) was performed using synthetic TF-polyacrylamide conjugate as antigen, total IgG purified by affinity chromatography on protein G sepharose, and lectins of various sugar specificities: mannose-specific concanavalin A (ConA), fucose-specificAleuria aurantia lectin (AAL) and sialic acid-specific Sambucus nigra agglutinin (SNA). The sensitivity and specificity of the differences between cancer patients and controls were evaluated by receiver operator characteristic (ROC) curve analysis. Overall survival was analyzed by the Kaplan-Meier method. Time-dependent ROC curve statistics were applied to determine cut-off values for survival analysis. All calculations and comparisons were performed using the GraphPad Prism 5 and SPSS 15.0 software.RESULTS: The level of TF-specific IgG was significantly increased in cancer patients compared with non-cancer controls (P < 0.001). This increase was pronounced mostly in stage 1 of the disease. Cancer patients showed a higher level of ConA binding to antiTF-IgG (P < 0.05) and a very low level of SNA lectin binding (P = 0.0001). No appreciable stage-dependency of the binding of any lectin to anti-TF IgG was found. A strong positive correlation between the binding of AAL and SNA was found in all groups studied (r = 0.71-0.72; P < 0.0001). The changes in ConA reactivity were not related to those of the fucoseor sialic acid-specific lectin. Changes in the SNA binding index and the ConA/SNA binding ratio demonstrated good sensitivity and specificity for stomach cancer: sensitivity 78.79% (95%CI: 61.09-91.02) and 72.73% (95%CI: 57.21-85.04); specificity 79.17 (95%CI: 65.01-89.53) and 88.64% (95%CI: 71.8-96.6), for the SNA binding index and the ConA/SNA binding ratio, respectively. The other combinations of lectins did not improve the accuracy of the assay. The low level of ConA-positive anti-TF IgG was associated with a survival benefit in cancer patients (HR = 1.56; 95%CI: 0.78-3.09; P = 0.19), especially in stages 3-4 of the disease (HR = 2.17; 95%CI: 0.98-4.79; P = 0.048). A significantly better survival rate was found in all cancer patients with a low reactivity of anti-TF IgG to the fucose-specific AAL lectin (HR = 2.39; 95%CI: 1.0-5.7;P = 0.038).CONCLUSION: The changes in the TF-specific IgG glycosylation pattern can be used as a biomarker for stomach cancer detection, and to predict patient survival. | Kristel Kodar Jelena Izotova Kersti Klaamas Boris Sergeyev Lilian Jrvekülg Oleg Kurtenkov | 2013 | World Journal of Gastroenterology2013，19，23： | 3 |
| 2 | Observation of chaotic polarization attractors from a graphene mode locked soliton fiber laser显示文摘We have demonstrated an all-fiber passively mode locked erbium-doped fiber laser(EDFL) based on graphene–polyvinyl-alcohol film. By watchfully adjusting the polarization controller, two different polarization attractors, including polarization locked vector solitons and a circular attractor, can be observed. This is first time, to the best of our knowledge, to explore the dynamics polarization attractors exhibited by a vector soliton generated from an EDFL based on graphene. | 赵畅 黄千千 Mohammed Al Araimi Aleksey Rozhin Sergey Sergeyev 牟成博 | 2019 | Chinese Optics Letters2019，17，2： | 2 |
| 3 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 4 | The classification of microstructures of clay soils 显示文摘 | Sergeyev Y M Grabowska-Olszewska B Osipov V I | 1980 | Journal of Microscopy1980，120，12： | 2 |
| 5 | Outcome of percutaneous surgery stratified according to body mass index and kidney stone size显示文摘 | Sergeyev I Koi PT Jacobs SL | 2007 | Surg Laparosc Endosc Percutan Tech2007，17，3： | 1 |
| 6 | Discriete coil-globule transition of large DNA induced by cations surfantant 显示文摘 | Melhikoov S M Sergeyev V G Yoshikawa K | 1995 | J Ant Chem Soc1995，117，9： | 1 |
| 7 | Recovery of traceability links between software documentation and source code显示文摘 | Marcus A Malefic J I Sergeyev A | 2005 | International Journal of Software Engineering and Knowledge Engineering(IJSEKE)2005，15，5： | 1 |
| 8 | Firms R&D decisions under incomplete information显示文摘 | Grishagin V A Sergeyev Y D Silipo D B | 2001 | European Journal of Operational Research2001，29，1： | 1 |
| 9 | Global optimization:fractal approach and non-redundant parallelism显示文摘 | Strongin R G Sergeyev Y D | 2003 | Journal of Global Optimization2003，27，： | 1 |
| 10 | Discrete coil-globule transition of large DNA induced by cationic surfactant 显示文摘 | MELNIKOV S M SERGEYEV V G YOSHIKAWA K | 1995 | J Am Chem Soc1995，117，9： | 1 |
| 11 | Sensitivity analysis and optimal design of 3D frame structures for stress and frequency constraints 显示文摘 | Sergeyev O Morz Z | 2000 | Computers & Structures2000，75，： | 1 |
| 12 | Outcome of percutaneous surgery stratified according to body mass index and kidney stone size显示文摘 | Sergeyev I Koi PT Jacobs SL | | 0，，03： | 1 |
| 13 | Preparation and use of hyperimmune serum for prophylax- is and therapy of ebola virus infection 显示文摘 | Kudoyarova-Zubavichene NM Sergeyev NN Chepurnov AA | 1999 | J Infect Dis1999，179，1： | 1 |
| 14 | A model system to quantitate tumor bur-den in locoregional lymph nodes during cancer spread显示文摘 | Moore A Sergeyev N Bredow S | 1998 | Invasion Metastasis1998，18，4： | 1 |
| 15 | The use of discrete characters in discriminant analysis for diagnosis of pulmonary tuberculosis and for classification of patients differing in treatment efficiency based on polymorphisms at nine codominant loci:HP,GC,TF,PI,PGM1,GLO1,C3,ACP1,and ESD显示文摘 | SERGEYEV AS AGAPOVA RK BOGADELNIKOVA IV | 2003 | Russian J Genetics2003，39，7： | 1 |
| 16 | Immunoglobulin G Fc N-glycan profiling in patients with gastric cancer by LC-ESI-MS: relation to tumor progression and survival显示文摘 | Kristel Kodar Johannes Stadlmann Kersti Klaamas Boris Sergeyev Oleg Kurtenkov | 2012 | Glycoconjugate Journal2012，，1： | 1 |
| 17 | Discotic liquid crystals: A new generation of organic semioonduetors 显示文摘 | Sergeyev S Pisula W Geerts Y H | 2007 | Chemcial Society Review2007，36，12： | 1 |
| 18 | Spiral attractor created by vector solitons显示文摘Mode-locked lasers emitting a train of femtosecond pulses called dissipative solitons are an enabling technology for metrology,high-resolution spectroscopy,fibre optic communications,nano-optics and many other fields of science and applications.Recently,the vector nature of dissipative solitons has been exploited to demonstrate mode locked lasing with both locked and rapidly evolving states of polarisation.Here,for an erbium-doped fibre laser mode locked with carbon nanotubes,we demonstrate the first experimental and theoretical evidence of a new class of slowly evolving vector solitons characterized by a double-scroll chaotic polarisation attractor substantially different from Lorenz,Ro¨ssler and Ikeda strange attractors.The underlying physics comprises a long time scale coherent coupling of two polarisation modes.The observed phenomena,apart from the fundamental interest,provide a base for advances in secure communications,trapping and manipulation of atoms and nanoparticles,control of magnetisation in data storage devices and many other areas. | Sergey V Sergeyev Chengbo Mou Elena G Turitsyna Alexey Rozhin Sergei K Turitsyn Keith Blow | 2014 | Light(Science & Applications)2014，3，1： | 1 |
| 19 | An efficient algorithm for the zero crossing detection in digitized measurement signal显示文摘 | A Molinaro Y D Sergeyev | 2001 | Measurement2001，30，3： | 1 |
| 20 | Determination of the three-dimensional structure of oligosaccharides in the solid state from experimental ^13C NMR data and ab initio chemical shift surfaces显示文摘 | Sergeyev I Moyna G | 2005 | Carbohydrate Research2005，340，6： | 1 |
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