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| 1 | Aberrant glycosylation of the anti-Thomsen-Friedenreich glycotope immunoglobulin G in gastric cancer patients显示文摘AIM: To study whether alterations in the glycosylation of immunoglobulin G (IgG) specific to the ThomsenFriedenreich glycotope (TF) have diagnostic and prognostic potential in gastric cancer. METHODS: Serum samples were obtained from patients with histologically verified gastric carcinoma (n = 89), healthy blood donors (n = 40), and patients with benign stomach diseases (n = 22). The lectin-enzymelinked immunosorbent assay-based glycoprofiling of TF-specific IgG (anti-TF IgG) was performed using synthetic TF-polyacrylamide conjugate as antigen, total IgG purified by affinity chromatography on protein G sepharose, and lectins of various sugar specificities: mannose-specific concanavalin A (ConA), fucose-specificAleuria aurantia lectin (AAL) and sialic acid-specific Sambucus nigra agglutinin (SNA). The sensitivity and specificity of the differences between cancer patients and controls were evaluated by receiver operator characteristic (ROC) curve analysis. Overall survival was analyzed by the Kaplan-Meier method. Time-dependent ROC curve statistics were applied to determine cut-off values for survival analysis. All calculations and comparisons were performed using the GraphPad Prism 5 and SPSS 15.0 software.RESULTS: The level of TF-specific IgG was significantly increased in cancer patients compared with non-cancer controls (P < 0.001). This increase was pronounced mostly in stage 1 of the disease. Cancer patients showed a higher level of ConA binding to antiTF-IgG (P < 0.05) and a very low level of SNA lectin binding (P = 0.0001). No appreciable stage-dependency of the binding of any lectin to anti-TF IgG was found. A strong positive correlation between the binding of AAL and SNA was found in all groups studied (r = 0.71-0.72; P < 0.0001). The changes in ConA reactivity were not related to those of the fucoseor sialic acid-specific lectin. Changes in the SNA binding index and the ConA/SNA binding ratio demonstrated good sensitivity and specificity for stomach cancer: sensitivity 78.79% (95%CI: 61.09-91.02) and 72.73% (95%CI: 57.21-85.04); specificity 79.17 (95%CI: 65.01-89.53) and 88.64% (95%CI: 71.8-96.6), for the SNA binding index and the ConA/SNA binding ratio, respectively. The other combinations of lectins did not improve the accuracy of the assay. The low level of ConA-positive anti-TF IgG was associated with a survival benefit in cancer patients (HR = 1.56; 95%CI: 0.78-3.09; P = 0.19), especially in stages 3-4 of the disease (HR = 2.17; 95%CI: 0.98-4.79; P = 0.048). A significantly better survival rate was found in all cancer patients with a low reactivity of anti-TF IgG to the fucose-specific AAL lectin (HR = 2.39; 95%CI: 1.0-5.7;P = 0.038).CONCLUSION: The changes in the TF-specific IgG glycosylation pattern can be used as a biomarker for stomach cancer detection, and to predict patient survival. | Kristel Kodar Jelena Izotova Kersti Klaamas Boris Sergeyev Lilian Jrvekülg Oleg Kurtenkov | 2013 | World Journal of Gastroenterology2013，19，23： | 3 |
| 2 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 3 | Effect of Mo,Se,Zn and Cr treatment on the yield, element concentration and carotenoid Agric显示文摘 | Bicas P A Daood H G Kodar I | 2001 | Food Chem2001，43，： | 1 |
| 4 | effect of Mo,Se,Zn and Cr treatments on the yield显示文摘 | Biacs P A Dood H G KOdar I | 1995 | Agric food Chen1995，43，12： | 1 |
| 5 | An increased level of the Concanavalin A-positive IgG in the serum of patients with gastric cancer as evaluated by a lectin enzyme-linked immunosorbent assay (LELISA)显示文摘 | Klaamas K Kodar K Kurtenkov O | 2008 | Neoplasma2008，55，2： | 1 |
| 6 | An increased level of the Concanavalin A-positive IgG in the serum of patients with gastric cancer as evaluated by a lectin enzyme-linked immunosorbent assay (LELISA)显示文摘 | Klaamas K Kodar K Kurtenkov O | 2008 | Neoplasma2008，55，2： | 1 |
| 7 | Microbubble contrast agent for Color Doppler US:Effect on breast masses, Work in progress显示文摘 | Kodar R P Cosgrove D Mc Cready V R | 2006 | Radiology2006，198，3： | 1 |
| 8 | The Pivotal Role of TNF -α in Rheumatoid Arthritis显示文摘As a main pro-inflammatory factor, the up-regulation of TNF-α in RA patients plays a pivotal role in RA pathogenesis. The process of TNF production starts with the binding of a ligand( which is commonly a microbial product) to a cell surface Toll receptor, which stimulates a signal transduction pathway that activates NF - κB transcription factors. Activated NF - κB enters nuclei and induces the transcription of genes associated with inflammation, including those coding for TNF. The production of TNF and other inflammatory cytokines serves to recruit other inflammatory cells, which in turn release cytokines and subsequently amplify the immune response. TNF exerts its effect via binding of TNFRland TNFR2 receptors. Many experimental and clinical study have proved the effectiveness of TNF - αa ctivity blockade. Recent therapeutic interventions, including TNF - α and IL - 1 inhibitors, strongly support the importance of cytokines in RA. The mechanism of anti - TNF - α action in vivo is complex and probably includes suppression of other pro - inflammatory cytokines, decreased synovial cellular infiltration, interference with osteoclast activation, and decreased angiogenisis. Studies using anti -TNF agents clearly show that they can slow or prevent the progression of bone and cartilage damage in RA. This activity probably involves suppression of osteoclasts in joint lesions. Etanereept, Infliximab and adalimumab are the three main biologic agents that were fo-cused on clinically at present. | Kodar Lee | 2004 | 中医药学刊2004，22，10： | 1 |
| 9 | Immunoglobulin G Fc N-glycan profiling in patients with gastric cancer by LC-ESI-MS: relation to tumor progression and survival显示文摘 | Kristel Kodar Johannes Stadlmann Kersti Klaamas Boris Sergeyev Oleg Kurtenkov | 2012 | Glycoconjugate Journal2012，，1： | 1 |
| 10 | Effect of Mo,Se,Zn and Cr treatments on the yield, element concentration, and carotenoid ontent of carrot显示文摘 | Bicas P A Daood H G Kodar I | 1995 | Agric Food Chem1995，43，： | 1 |
| 11 | Effeet of Mo, Se, Zn and Cr treatments on the yield, element concentration and carotenoid content of car rot显示文摘 | Biacs P A Dood H G Kodar I | 1995 | J Agr Food Chem1995，43，12： | 1 |
| 12 | 停不住地聊天! 超年轻魅力YouTuber kemio显示文摘Vine、Twitter、Instagram还有YouTube。现在年轻人无人不知的人气网红就是kemio。这个月附录中的贴纸也是他的格言,Kemio拥有平成独特的魅力。——1995年出生的你眼中的平成是?我出生的时候就是平成,所以没有记忆了,1990年代的记忆几乎都是零。小时候的事情都没什么印象了,所以软盘那些,数字化的改变我都没有感觉。小学时候的话印象最深的就是早安少女组合,超级喜欢她们.我很喜欢偶像的,还会学她们跳舞。然后高中时候拼命打工。仿佛这辈子只有赚钱。 | Kodar | 2019 | 美眉2019，0，5： | 0 |
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