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| 1 | Dihydromyricetin and Salvianolic acid B inhibit alpha-synuclein aggregation and enhance chaperone-mediated autophagy显示文摘Background:Progressive accumulation ofα-synuclein is a key step in the pathological development of Parkinson’s disease.Impaired protein degradation and increased levels ofα-synuclein may trigger a pathological aggregation in vitro and in vivo.The chaperone-mediated autophagy(CMA)pathway is involved in the intracellular degradation processes ofα-synuclein.Dysfunction of the CMA pathway impairsα-synuclein degradation and causes cytotoxicity.Results:In the present study,we investigated the effects on the CMA pathway andα-synuclein aggregation using bioactive ingredients(Dihydromyricetin(DHM)and Salvianolic acid B(Sal B))extracted from natural medicinal plants.In both cell-free and cellular models ofα-synuclein aggregation,after administration of DHM and Sal B,we observed significant inhibition ofα-synuclein accumulation and aggregation.Cells were co-transfected with a Cterminal modifiedα-synuclein(SynT)and synphilin-1,and then treated with DHM(10μM)and Sal B(50μM)16 hours after transfection;levels ofα-synuclein aggregation decreased significantly(68%for DHM and 75%for Sal B).Concomitantly,we detected increased levels of LAMP-1(a marker of lysosomal homeostasis)and LAMP-2A(a key marker of CMA).Immunofluorescence analyses showed increased colocalization between LAMP-1 and LAMP-2A withα-synuclein inclusions after treatment with DHM and Sal B.We also found increased levels of LAMP-1 and LAMP-2A both in vitro and in vivo,along with decreased levels ofα-synuclein.Moreover,DHM and Sal B treatments exhibited anti-inflammatory activities,preventing astroglia-and microglia-mediated neuroinflammation in BAC-α-syn-GFP transgenic mice.Conclusions:Our data indicate that DHM and Sal B are effective in modulatingα-synuclein accumulation and aggregate formation and augmenting activation of CMA,holding potential for the treatment of Parkinson’s disease. | Jia-Zhen Wu Mustafa Ardah Caroline Haikal Alexander Svanbergsson Meike Diepenbroek Nishant N.Vaikath Wen Li Zhan-You Wang Tiago F.Outeiro Omar M.El-Agnaf Jia-Yi Li | 2019 | Translational Neurodegeneration2019，8，1： | 11 |
| 2 | CAD and CAE Integration Through Scientific Visualization Techniques for Illumination Design显示文摘Engineers tend to use different software to perform tasks such as geometry modeling, database management, numerical analysis, and visualization. This may cause decrease of productivity and loss of information during the conversion process between different data file formats. This paper presents a computer aided design (CAD) and computer aided engineering (CAE) system integration using scientific visualization tools and techniques. It deals with the development of a 3D CAD add-in for lighting analysis which uses the CAD model as 3D interface for creating a lighting scheme, processing, and visualizing 2D or 3D illuminance fields. Visualization features as color and contour mapping were developed using the visualization toolkit (VTK) toolkit. The application integrates all functionalities of the 3D CAD with tools for light sources database management, pre-processing, processing, and post-processing of illuminance fields in a single environment. This approach increases productivity and eliminates the need for different software. | Tiago Martinuzzi Buriol Sergio Scheer | 2008 | Tsinghua Science and Technology2008，13，S1： | 10 |
| 3 | Familial aggregation in inflammatory bowel disease:Is it genes or environment?显示文摘Inflammatory bowel disease (IBD) develops in genetically susceptible individuals due to the influence of environmental factors, leading to an abnormal recognition of microbiota antigens by the innate immune system which triggers an exaggerated immune response and subsequent bowel tissue damage. IBD has been more frequently found in families, an observation that could be due to either genetic, environmental or both types of factors present in these families. In addition to expanding our knowledge on IBD pathogenesis, defining the specific contribution to familial IBD of each one of these factors might have also clinical usefulness. We review the available evidence on familial IBD pathogenesis. | Tiago Nunes Gionata Fiorino Silvio Danese Miquel Sans | 2011 | World Journal of Gastroenterology2011，17，22： | 9 |
| 4 | Exercise training in hypertension:Role of microRNAs显示文摘Hypertension is a complex disease that constitutes an important public health problem and demands many studies in order to understand the molecular mechanisms involving his pathophysiology. Therefore, an increasing number of studies have been conducted and new therapies are continually being discovered. In this context, exercise training has emerged as an important non-pharmacological therapy to treat hypertensive patients, minimizing the side effects of pharmacological therapies and frequently contributing to allow pharmacotherapy to be suspended. Several mechanisms have been associated with the pathogenesis of hypertension, such as hyperactivity of the sympathetic nervous system and renin-angiotensin aldosterone system,impaired endothelial nitric oxide production, increased oxygen-reactive species, vascular thickening and stiffening, cardiac hypertrophy, impaired angiogenesis, and sometimes genetic predisposition. With the advent of microRNAs(miRNAs), new insights have been added to the perspectives for the treatment of this disease, and exercise training has been shown to be able to modulate the miRNAs associated with it. Elucidation of the relationship between exercise training and miRNAs in the pathogenesis of hypertension is fundamental in order to understand how exercise modulates the cardiovascular system at genetic level. This can be promising even for the development of new drugs. This article is a review of how exercise training acts on hypertension by means of specific miRNAs in the heart, vascular system, and skeletal muscle. | Vander Josédas Neves Tiago Fernandes Fernanda Roberta Roque Ursula Paula RenóSoci Stéphano Freitas Soares Melo Edilamar Menezes de Oliveira | 2014 | World Journal of Cardiology2014，6，8： | 9 |
| 5 | Nature:通过控制血管生长来开发治疗癌症等多种疾病的新型疗法显示文摘最近,一项刊登在国际杂志Nature上的研究报告中,来自耶鲁大学的研究人员通过研究揭示了如何通过控制血管发育来帮助开发治疗心血管疾病以及癌症的新型疗法。 | Yu, Pengchun Wilhelm, Kerstin Dubrac, Alexandre Tung, Joe K. Alves, Tiago C. Fang, Jennifer S. Xie, Yi Zhu, Jie Chen, Zehua De Smet, Frederik Zhang, Jiasheng Jin, Suk-Won Sun, Lele Sun, Hongye Kibbey, Richard G. Hirschi, Karen K. Hay, Nissim Carmeliet, Peter Chittenden, Thomas W. Eichmann, Anne Potente, Michael Simons, Michael | 2017 | 现代生物医学进展2017，17，22： | 9 |
| 6 | 以用户为中心的新兴电力市场模式显示文摘随着可再生能源在现代能源产业中的占比不断提高,其对现行电力系统和电力市场的运行机制所带来的影响也日趋明显;同时,通过与数字信息产业深度结合,分布式能量管理手段也处于飞速发展阶段。在这二者的驱动下,一种以用户为中心的新兴电力市场模式正以不同的表现形式出现在世界各地。文章以高度概括的方式介绍了这一新兴电力市场模式,浅析了其在不同表现形式下的机理和特征。基于这一新兴电力市场模式在短期内所取得的发展成就,有理由相信此类新兴电力市场模式将逐步成为未来电力市场中的重要组成部分。 | Pierre Pinson Thomas Baroche Fabio Moret Tiago Sousa Etienne Sorin Shi You | 2017 | 供用电2017，34，12： | 6 |
| 7 | The Use of Data Mining Techniques in Rockburst Risk Assessment显示文摘目前在世界范围内的很多地下矿山,岩爆已经成为一个与矿山采矿生产密切相关的重要现象。深入理解这类现象,不仅有助于岩爆管理,而且还有可能节约采矿成本,减少人身伤亡事故。其中,实验室实验是深入研究岩爆机理的一个重要途径。在本文作者前期的研究中,已经建立了实验室岩爆实验数据库。与此同时,借助于数字采矿技术,也建立了岩爆最大应力和岩爆风险指数的预测模型。为实现基于矿山地质条件和矿山井巷建筑结构特性对岩爆类型即岩爆强度等级的准确预测,本文的重点是,基于对岩爆实例的分析来建立岩爆影响矩阵,明确岩爆现象的诱发因子,并厘清这些影响因子之间的相互关系。运用人工神经网络(ANN)和初始贝叶斯分类器等数字矿山技术,对矿山岩爆数据库进行了更深入的研究。最后给出了研究得出的各项结论。 | Luis Ribeiro e Sousa Tiago Miranda Rita Leal e Sousa Joaquim Tinoco | 2017 | Engineering2017，3，4： | 6 |
| 8 | Fluoroquinolones for the treatment of latent Mycobacterium tuberculosis infection in liver transplantation显示文摘Solid organ transplantation(SOT)is the best treatment option for end-stage organ disease.Newer immunosuppressive agents have reduced the incidence of graft rejection but have increased the risk of infection,particularly due to the reactivation of latent infections due to opportunistic agents such as Mycobacterium tuberculosis.Active tuberculosis(TB)after SOT is a significant cause of morbidity and mortality.Most cases of posttransplant TB are secondary to reactivation of latent tuberculosis infection(LTBI)due to the effects of long-term immunosuppressive therapy.Risk minimization strategies have been developed to diagnose LTBI and initiate treatment prior to transplantation.Isoniazid with vitamin B6 supplementation is the treatment of choice.However,liver transplantation(LT)candidates and recipients have an increased risk of isoniazid-induced liver toxicity,leading to lower treatment completion rates than in other SOT populations.Fluoroquinolones(FQs)exhibit good in vitro antimycobacterial activity and a lower risk of drug-induced liver injury than isoniazid.In the present review,we highlight the disease burden posed by posttransplant TB and summarize the emerging clinical evidence supporting the use of FQs for the treatment of LTBI in LT recipients and candidates. | Jose Tiago Silva Rafael San-Juan Mario Fernández-Ruiz José María Aguado | 2019 | World Journal of Gastroenterology2019，25，26： | 5 |
| 9 | Postpartum depression:A systematic review of the genetics involved显示文摘Postpartum depression is one of the most prevalent psychopathologies. Its prevalence is estimated to be between 10% and 15%. Despite its multifactorial etiology, it is known that genetics play an important role in the genesis of this disorder. This paper reviews epidemiological evidence supporting the role of genetics in postpartum depression(PPD). The main objectives of this review are to determine which genes and polymorphisms are associated with PPD and discuss how this association may occur. In addition, this paper explores whether these genes are somehow related to or even the same as those linked to Major Depression(MD). To identify gaps in the current knowledge that require investigation, a systematic review was conducted in the electronic databases Pub Med, LILACS and Sci ELO using the index terms 'postpartum depression' and 'genetics'. Literature searches for articles in peerreviewed journals were made until April 2014. PPD was indexed 56 times with genetics. The inclusion criteria were articles in Portuguese, Spanish or English that were available by institutional means or sent by authors upon request; this search resulted in 20 papers. Genes and polymorphisms traditionally related to MD, which are those involved in the serotonin, catecholamine, brain-derived neurotrophic factor and tryptophan metabolism, have been the most studied, and some have been related to PPD. The results are conflicting and some depend on epigenetics, which makes the data incipient. Further studies are required to determine the genes that are involved in PPD and establish the nature of the relationship between these genes and PPD. | Tiago Castro e Couto Mayra Yara Martins Brancaglion António Alvim-Soares Lafaiete Moreira Frederico Duarte Garcia Rodrigo Nicolato Humberto Corrêa Regina Amélia Lopes P Aguiar Henrique Vitor Leite | 2015 | World Journal of Psychiatry2015，5，1： | 5 |
| 10 | Transjugular intrahepatic portosystemic shunt for Budd-Chiari syndrome:A comprehensive review显示文摘Budd-Chiari syndrome(BCS)is a relatively rare clinical condition with a wide range of symptomatology,caused by the obstruction of the hepatic venous outflow.If left untreated,it has got an high mortality rate.Its management is based on a step-wise approach,depending on the clinical presentation,and includes different treatment from anticoagulation therapy up to Interventional Radiology techniques,such as transjugular intrahepatic portosystemic shunt(TIPS).TIPS is today considered a safe and highly effective treatment and should be recommended for BCS patients,including those awaiting orthotopic liver transplantation.In this review the pathophysiology,diagnosis and treatment options of BCS are presented,with a special focus on published data regarding the techniques and outcomes of TIPS for the treatment of BCS.Moreover,unresolved issues and future research will be discussed. | Riccardo Inchingolo Alessandro Posa Martin Mariappan Tiago Kojun Tibana Thiago Franchi Nunes Stavros Spiliopoulos Elias Brountzos | 2020 | World Journal of Gastroenterology2020，26，34： | 5 |
| 11 | 急性缺血性卒中的机械取栓治疗:ESO、ESMINT、ESNR和EAN共同支持的2014/2015 ESO-卡罗林斯卡卒中更新会议共识声明显示文摘这份卒中后机械取栓治疗共识声明的原始版本于2014年11月16—18日在斯德哥尔摩召开的欧洲卒中组织(European Stroke Organisation, ESO)-卡罗林斯卡卒中更新会议期间得到批准。根据会议安排,在2015年利用新的临床试验数据对该声明进行了更新。2015年2月,在瑞士伯尔尼召开的ESO冬季培训会期间组织了面对面会议对共识声明进行修订,然后通过电子邮件交换意见,最终版本得到各学会的认可。推荐意见与原始版本一致,证据等级更新到2015年2月20日为止,在2015年5月15日获得确认。ESO-卡罗林斯卡卒中更新会议的目的在于总结卒中治疗研究的最新进展并探讨如何将这些最新研究结果应用于临床实践。共识会议选择了一些议题进行讨论,与会者草拟一份共识声明并进行讨论。该声明接受了ESO指南委员会的指导。这份共识声明包括对急性卒中后机械取栓治疗的推荐意见,得到欧洲卒中组织ESO、欧洲神经微创治疗学会(European Society of Minimally Invasive Neurological Therapy, ESMINT)、欧洲神经放射学学会(European Society of Neuroradiology, ESNR)和欧洲神经病学学会(European Academy of Neurology, EAN)的支持。 | Nils Wahlgren Tiago Moreira Patrik Michel Thorsten Steiner Olav Jansen Christophe Cognard Heinrich P Mattle Wim van Zwam Staffan Holmin Turgut Tatlisumak Jesper Petersson Valeria Caso Werner Hacke Mikael Mazighi Marcel Arnold Urs Fischer Istvan Szikora Laurent Pierot Jens Fiehler Jan Gralla Franz Fazekas Kennedy R Lees 高洁 徐格林 | 2016 | 国际脑血管病杂志2016，24，1： | 5 |
| 12 | Upper gastrointestinal bleeding risk scores: Who, when andwhy?显示文摘Upper gastrointestinal bleeding(UGIB) remains a significant cause of hospital admission. In order to stratify patients according to the risk of the compli-cations, such as rebleeding or death, and to predict the need of clinical intervention, several risk scores have been proposed and their use consistently recommended by international guidelines. The use of risk scoring systems in early assessment of patients suffering from UGIB may be useful to distinguish high-risks patients, who may need clinical intervention and hospitalization, from low risk patients with a lower chance of developing complications, in which management as outpatients can be considered. Although several scores have been published and validated for predicting different outcomes, the most frequently cited ones are the Rockall score and the Glasgow Blatchford score(GBS). While Rockall score, which incorporates clinical and endoscopic variables, has been validated to predict mortality, the GBS, which is based on clinical and laboratorial parameters, has been studied to predict the need of clinical intervention. Despite the advantages previously reported, their use in clinical decisions is still limited. This review describes the different risk scores used in the UGIB setting, highlights the most important research, explains why and when their use may be helpful, reflects on the problems that remain unresolved and guides future research with practical impact. | Sara Monteiro Tiago Cúrdia Gonçalves Joana Magalhães JoséCotter | 2016 | World Journal of Gastrointestinal Pathophysiology2016，7，1： | 5 |
| 13 | High prevalence of vitamin A deficiency in Crohn's disease patients according to serum retinol levels and the relative dose-response test显示文摘AIM:To assess the vitamin A status of patients with Crohn's disease(CD) by evaluating serum retinol levels and the relative dose response(RDR) test(liver retinol stores).METHODS:Vitamin A nutritional status was measured by serum retinol obtained by high performance liquid chromatography and the RDR test for evaluation of the hepatic stores.Body composition was performed by densitometry by dual-energy X-ray absorptiometry.Vitamin A dietary intake was assessed from a semiquantitative food frequency questionnaire.RESULTS:This study included 38 CD patients and 33 controls.Low serum retinol concentrations were detected in 29% of CD patients vs 15% in controls(P < 0.005).The RDR test was positive in 37% of CD patients vs 12% in controls,which indicated inadequate hepatic vitamin A stores(P < 0.005).Individuals with hypovitaminosis A had lower BMI and body fat compared with those without this deficiency.There was no association between vitamin A deficiency and its dietary intake,ileal location,presence of disease activity and prior bowel resections.CONCLUSION:Patients with CD have higher prevalence of vitamin A deficiency,as assessed by two independent methods. | Márcia Soares-Mota Tianny A Silva Luanda M Gomes Marco AS Pinto Laura MC Mendon?a Maria Lúcia F Farias Tiago Nunes Andrea Ramalho Cyrla Zaltman | 2015 | World Journal of Gastroenterology2015，21，5： | 4 |
| 14 | 2024 Adult Compendium of Physical Activities:A third update of the energy costs of human activities显示文摘Background:The Compendium of Physical Activities was published in 1993 to improve the comparability of energy expenditure values assigned to self-reported physical activity(PA)across studies.The original version was updated in 2000,and again in 2011,and has been widely used to support PA research,practice,and public health guidelines.Methods:This 2024 update was tailored for adults 19-59 years of age by removing data from those≥60 years.Using a systematic review and supplementary searches,we identified new activities and their associated measured metabolic equivalent(MET)values(using indirect calorimetry)published since 2011.We replaced estimated METs with measured values when possible.Results:We screened 32,173 abstracts and 1507 full-text papers and extracted 2356 PA energy expenditure values from 701 papers.We added303 new PAs and adjusted 176 existing MET values and descriptions to reflect the addition of new data and removal of METs for older adults.We added a Major Heading(Video Games).The 2024 Adult Compendium includes 1114 PAs(912 with measured and 202 with estimated values)across 22 Major Headings.Conclusion:This comprehensive update and refinement led to the creation of The 2024 Adult Compendium,which has utility across research,public health,education,and healthcare domains,as well as in the development of consumer health technologies.The new website with the complete lists of PAs and supporting resources is available at http://gffzz09a845152db44f16s0xpoonfbuxkq6fuv.ffgz.tsg.suse.edu.cn. | Stephen D.Herrmann Erik A.Willis Barbara E.Ainsworth Tiago V.Barreira Mary Hastert Chelsea L.Kracht John M.Schuna Jr. Zhenghui Cai Minghui Quan Catrine Tudor-Locke Melicia C.Whitt-Glover David R. Jacobs Jr. | 2024 | Journal of Sport and Health Science2024，13，1： | 4 |
| 15 | Prevalence and risk factors of infections by multiresistant bacteria in cirrhosis: A prospective study显示文摘 | Javier Fernández Juan Acevedo Miriam Castro Orlando Garcia Carlos Rodríguez de Lope Daria Roca Marco Pavesi Elsa Sola Leticia Moreira Anibal Silva Tiago Seva‐Pereira Francesco Corradi Jose Mensa Pere Ginès Vicente Arroyo | 2012 | Hepatology2012，，： | 4 |
| 16 | N-Acetyltransferase 2 genetic polymorphisms and risk of colorectal cancer显示文摘AIM:To investigate the possible association between meat intake,cigarette smoking and N-acetyltransferase 2 (NAT2) genetic polymorphisms on colorectal cancer (CRC) risk.METHODS:Patients with CRC were matched for gender and age to healthy controls.Meat intake and cigarette smoking were assessed using a specific frequency questionnaire.DNA was extracted from peripheral blood and the genotypes of the polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism.Five NAT2 alleles were studied (WT,M1,M2,M3 and M4) using specific digestion enzymes.RESULTS:A total of 147 patients with colorectal cancer (76 women and 90 men with colon cancer) and 212 controls were studied.The mean age of the two groups was 62 years.More than half the subjects (59.8% in the case group and 51.9% in the control group) were NAT2 slow acetylators.The odds ratio for colorectal cancer was 1.38 (95% CI:0.90-2.12) in slow acetylators.Although the number of women was small (n=76 in the case group),the cancer risk was found to be lower in intermediate (W/Mx) acetylators [odds ratio (OR):0.55,95% confidence interval (95% CI):0.29-1.02].This difference was not observed in men (OR:0.56,95% CI:0.16-2.00).Among NAT2 fast acetylators (W/W or W/Mx),meat consumption more than 3 times a week increased the risk of colorectal cancer (OR:2.05,95% CI:1.01-4.16).In contrast,cigarette smoking increased the risk of CRC among slow acetylators (OR:1.97,95% CI:1.02-3.79).CONCLUSION:The risk of CRC was higher among fast acetylators who reported a higher meat intake.Slow NAT2 acetylation was associated with an increased risk of CRC. | Tiago Donizetti da Silva Aledson Vitor Felipe Jacqueline Miranda de Lima Celina Tizuko Fujiyama Oshima Nora Manoukian Forones | 2011 | World Journal of Gastroenterology2011，17，6： | 3 |
| 17 | Dealing with robot-assisted surgery for rectal cancer:current status and perspectives显示文摘The laparoscopic approach for treatment of rectal cancer has been proven feasible and oncologically safe, and is able to offer better short-term outcomes than traditional open procedures, mainly in terms of reduced length of hospital stay and time to return to working activity. In spite of this, the laparoscopic technique is usually practised only in high-volume experienced centres, mainly because it requires a prolonged and demanding learning curve. It has been estimated that over 50 operations are required for an experienced colorectal surgeon to achieve proficiency with this technique. Robotic surgery enables the surgeon to perform minimally invasive operations with better vision and more intuitive and precise control of the operating instruments, thus promising to overcome some of the technical difficulties associated with standard laparoscopy. It has high-definition threedimensional vision, it translates the surgeon's hand movements into precise movements of the instruments inside the patient, the camera is held and moved by the first surgeon, and a fourth robotic arm is available as a fixed retractor. The aim of this review is to summarise the current data on clinical and oncologic outcomes of robot-assisted surgery in rectal cancer, focusing on short- and long-term results, and providing original data from the authors' centre. | Roberto Biffi Fabrizio Luca Paolo Pietro Bianchi Sabina Cenciarelli Wanda Petz Igor Monsellato Manuela Valvo Maria Laura Cossu Tiago Leal Ghezzi Kassem Shmaissany | 2016 | World Journal of Gastroenterology2016，22，2： | 3 |
| 18 | Older Adult Compendium of Physical Activities:Energy costs of human activities in adults aged 60 and older显示文摘Purpose:To describe the development of a Compendium for estimating the energy costs of activities in adults>60 years(OA Compendium).Methods:Physical activities(PAs)and their metabolic equivalent of task(MET)values were obtained from a systematic search of studies published in 4 sport and exercise databases(PubMed,Embase,SPORTDiscus(EBSCOhost),and Scopus)and a review of articles included in the 2011 Adult Compendium that measured PA in older adults.MET values were computed as the oxygen cost(VO_(2),mL/kg/min)during PA divided by 2.7 m L/kg/min(MET_(60+))to account for the lower resting metabolic rate in older adults.Results:We identified 68 articles and extracted energy expenditure data on 427 PAs.From these,we derived 99 unique Specific Activity codes with corresponding MET_(60+)values for older adults.We developed a website to present the OA Compendium MET_(60+)values:http://gffzz20a12edcdf3c446fs0xpoonfbuxkq6fuv.ffgz.tsg.suse.edu.cnThe OA Compendium uses data collected from adults>60 years for more accurate estimation of the energy cost of PAs in older adults.It is an accessible resource that will allow researchers,educators,and practitioners to find MET_(60+)values for older adults for use in PA research and practice. | Erik A.Willis Stephen D.Herrmann Mary Hastert Chelsea L.Kracht Tiago V.Barreira John M.Schuna Jr. Zhenghua Cai Minghui Quan Scott A.Conger Wendy J.Brown Barbara E.Ainsworth | 2024 | Journal of Sport and Health Science2024，13，1： | 3 |
| 19 | Assessing the relationship between the rumen microbiota and feed efficiency in Nellore steers显示文摘Background:Ruminants rely upon a complex community of microbes in their rumen to convert host-indigestible feed into nutrients.However,little is known about the association between the rumen microbiota and feed efficiency traits in Nellore(Bos indicus)cattle,a breed of major economic importance to the global beef market.Here,we compare the composition of the bacterial,archaeal and fungal communities in the rumen of Nellore steers with high and low feed efficiency(FE)phenotypes,as measured by residual feed intake(RFI).Results:The Firmicutes to Bacteroidetes ratio was significantly higher(P<0.05)in positive-RFI steers(p-RFI,low feed efficiency)than in negative-RFI(n-RFI,high feed efficiency)steers.The differences in bacterial composition from steers with high and low FE were mainly associated with members of the families Lachnospiraceae,Ruminococcaceae and Christensenellaceae,as well as the genus Prevotella.Archaeal community richness was lower(P<0.05)in p-RFI than in n-RFI steers and the genus Methanobrevibacter was either increased or exclusive of p-RFI steers.The fungal genus Buwchfawromyces was more abundant in the rumen solid fraction of n-RFI steers(P<0.05)and a highly abundant OTU belonging to the genus Piromyces was also increased in the rumen microbiota of highefficiency steers.However,analysis of rumen fermentation variables and functional predictions indicated similar metabolic outputs for the microbiota of distinct FE groups.Conclusions:Our results demonstrate that differences in the ruminal microbiota of high and low FE Nellore steers comprise specific taxa from the bacterial,archaeal and fungal communities.Biomarker OTUs belonging to the genus Piromyces were identified in animals showing high feed efficiency,whereas among archaea,Methanobrevibacter was associated with steers classified as p-RFI.The identification of specific RFI-associated microorganisms in Nellore steers could guide further studies targeting the isolation and functional characterization of rumen microbes potentially important for the energy-harvesting efficiency of ruminants. | Déborah Romaskevis Gomes Lopes Márcio de Souza Duarte Alex J.La Reau Ibrahim Zuniga Chaves Tiago Antônio de Oliveira Mendes Edenio Detmann Cláudia Braga Pereira Bento Maria Eugênia Zerlotti Mercadante Sarah Figueiredo Martins Bonilha Garret Suen Hilário Cuquetto Mantovani | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 3 |
| 20 | Crohn's disease environmental factors in the developing world: A case-control study in a statewide catchment area in Brazil显示文摘AIM To identify environmental risk factors associated with the development of Crohn's disease(CD) in order to reassess the hygiene hypothesis.METHODS A hospital-based, case-control study was carried out with CD patients(n = 145) and controls(n = 163)representing a socioeconomically diverse statewide catchment area in Brazil. Controls were recruited from caregivers of patients seen in different outpatient clinics at the same hospital. A multi-item survey with 94 questions regarding family history of CD, perinatal and childhood circumstances, living conditions, tobacco use and familial socioeconomic status was carried out by interviewers. RESULTS On the univariate analysis, predictive variables for CD included being male, under age of 40, a high education level, urban dweller, smaller family size, exposure to enteric pathogens and user of treated water(P < 0.005). On the multivariate analysis, variables significantly associated with CD were male gender(OR = 2.09), under age 40(OR = 3.10), white(OR = 2.32), from a small family in childhood(OR = 2.34) and adulthood(OR = 3.02), absence of viral infections in childhood(OR = 2.23), exposure to enteric pathogens(OR = 2.41), having had an appendectomy(OR = 2.47) and prior or current smoker(OR = 2.83/1.12). CONCLUSION Most variables supporting the 'hygiene hypothesis' are associated with the development of CD but are not independent predictors of the diagnosis. | Valéria Cristina Loureiro Salgado Ronir Raggio Luiz Neio Boechat Bianca C Schorr Isabella S Leao Tiago Nunes Cyrla Zaltman | 2017 | World Journal of Gastroenterology2017，23，30： | 2 |
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