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| 1 | A Chromosome-Scale Genome Assembly of Paper Mulberry(Bmussonetia papyrifera)Provides New Insights into Its Forage and Papermaking Usage显示文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide. | Xianjun Peng Hui Liu Peilin Chen Feng Tang Yanmin Hu Fenfen Wang Zhi Pi Meiling Zhao Naizhi Chen Hui Chen Xiaokang Zhang Xueqing Yan Min Liu Xiaojun Fu Guofeng Zhao Pu Yao Lili Wang He Dai Xuming Li Wei Xiong Wencai Xu Hongkun Zheng Haiyan Yu Shihua Shen | 2019 | Molecular Plant2019，12，5： | 18 |
| 2 | Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)显示文摘Passion fruit(Passiflora edulis Sims)is an economically valuable fruit that is cultivated in tropical and subtropical regions of the world.Here,we report an~1341.7Mb chromosome-scale genome assembly of passion fruit,with 98.91%(~1327.18Mb)of the assembly assigned to nine pseudochromosomes.The genome includes 23,171 protein-coding genes,and most of the assembled sequences are repetitive sequences,with long-terminal repeats(LTRs)being the most abundant.Phylogenetic analysis revealed that passion fruit diverged after Brassicaceae and before Euphorbiaceae.Ks analysis showed that two whole-genome duplication events occurred in passion fruit at 65 MYA and 12 MYA,which may have contributed to its large genome size.An integrated analysis of genomic,transcriptomic,and metabolomic data showed that‘alpha-linolenic acid metabolism’,‘metabolic pathways’,and‘secondary metabolic pathways’were the main pathways involved in the synthesis of important volatile organic compounds(VOCs)in passion fruit,and this analysis identified some candidate genes,including GDP-fucose Transporter 1-like,Tetratricopeptide repeat protein 33,protein NETWORKED 4B isoform X1,and Golgin Subfamily A member 6-like protein 22.In addition,we identified 13 important gene families in fatty acid pathways and eight important gene families in terpene pathways.Gene family analysis showed that the ACX,ADH,ALDH,and HPL gene families,especially ACX13/14/15/20,ADH13/26/33,ALDH1/4/21,and HPL4/6,were the key genes for ester synthesis,while the TPS gene family,especially PeTPS2/3/4/24,was the key gene family for terpene synthesis.This work provides insights into genome evolution and flavor trait biology and offers valuable resources for the improved cultivation of passion fruit. | Zhiqiang Xia Dongmei Huang Shengkui Zhang Wenquan Wang Funing Ma Bin Wu Yi Xu Bingqiang Xu Di Chen Meiling Zou Huanyu Xu Xincheng Zhou Rulin Zhan Shun Song | 2021 | Horticulture Research2021，8，1： | 15 |
| 3 | MicroRNAs expression profiles in adipose tissues and liver from sex-linked dwarf and normal chickens显示文摘 | Yaqiong Ye Li Deng Meile Liang Lifeng Xu Lihua Zhang Yongjiang Ma Yugu Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，8： | 4 |
| 4 | Short-working-distance optical imaging system and method for surface detection of underwater structures显示文摘In the surface imaging of underwater structures, long working distance will reduce image quality due to the turbidity of water. To acquire high definition and large field of view(FOV) images for surface detection, a short-working-distance underwater imaging system is proposed based on camera array. A multi-view calibration and rectification method is developed. A look-up table(LUT) method and a multi-resolution spline(MRS) method are applied to stitch array images real-time and seamlessly.Experiments both in the air and in the water are conducted. Strength and weakness of the LUT and MRS methods are discussed.Based on the results, the effectiveness in surface detection of underwater structures is verified. | LIU Cong WANG ChengFei XU YingJun LI Xu LUO ChunHao DAI MeiLing SHAO XinXing HE XiaoYuan | 2018 | Science China(Technological Sciences)2018，61，5： | 4 |
| 5 | Frequency-domain diagonal extension imaging显示文摘The pixel size of a charge-coupled device(CCD)camera plays a major role in the image resolution,and the square pixels are attributed to the physical anisotropy of the sampling frequency.We synthesize the high sampling frequency directions from multiple frames acquired with different angles to enhance the resolution by 1.4×over conventional CCD orthogonal sampling.To directly demonstrate the improvement of frequency-domain diagonal extension(FDDE)microscopy,lens-free microscopy is used,as its resolution is dominantly determined by the pixel size.We demonstrate the resolution enhancement with a mouse skin histological specimen and a clinical blood smear sample.Further,FDDE is extended to lens-based photography with an ISO 12233 resolution target.This method paves a new way for enhancing the image resolution for a variety of imaging techniques in which the resolution is primarily limited by the sampling pixel size,for example,microscopy,photography,and spectroscopy. | Shan Jiang Meiling Guan Jiamin Wu Guocheng Fang Xinzhu Xu Dayong Jin Zhen Liu Kebin Shi Fan Bai Shu Wang Peng Xi | 2020 | Advanced Photonics2020，2，3： | 3 |
| 6 | The effects of altered BMP4 signaling in first branchialarch- derived murine embryonic orofacial tissues显示文摘The first branchial arch(BA1),which is derived from cranial neural crest(CNC)cells,gives rise to various orofacial tissues.Cre mice are widely used for the determination of CNC and exploration of gene functions in orofacial development.However,there is a lack of Cre mice specifically marked BA1’s cells.Pax2-Cre allele was previously generated and has been widely used in the field of inner ear development.Here,by compounding Pax2-Cre and R26R-mTmG mice,we found a specific expression pattern of Pax2^(+)cells that marked BA1’s mesenchymal cells and the BA1-derivatives.Compared to Pax2-Cre;R26R-mTmG allele,GFP^(+)cells were abundantly found both in BA1 and second branchial arch in Wnt1-Cre;R26R-mTmG mice.As BMP4 signaling is required for orofacial development,we over-activated Bmp4 by using Pax2-Cre;pMes-BMP4 strain.Interestingly,our results showed bilateral hyperplasia between the upper and lower teeth.We also compare the phenotypes of Wnt1-Cre;pMes-BMP4 and Pax2-Cre;pMes-BMP4 strains and found severe deformation of molar buds,palate,and maxilla-mandibular bony structures in Wnt1-Cre;pMes-BMP4 mice;however,the morphology of these orofacial organs were comparable between controls and Pax2-Cre;pMes-BMP4 mice except for bilateral hyperplastic tissues.We further explore the properties of the hyperplastic tissue and found it is not derived from Runx2^(+)cells but expresses Msx1,and probably caused by abnormal cell proliferation and altered expression pattern of p-Smad1/5/8.In sum,our findings suggest altering BMP4 signaling in BA1-specific cell lineage may lead to unique phenotypes in orofacial regions,further hinting that Pax2-Cre mice could be a new model for genetic manipulation of BA1-derived organogenesis in the orofacial region. | Jue Xu Meiling Chen Yanan Yan Qiaoxue Zhao Meiying Shao Zhen Huang | 2021 | International Journal of Oral Science2021，13，4： | 3 |
| 7 | Vacuum carburization of 12Cr2Ni4A low carbon alloy steel with lanthanum and cerium ion implantation显示文摘As bearing parts, 12 Cr2 Ni4 A is expected to have high hardness and excellent fatigue strength, so carburizing is employed to improve the inherit properties of 12 Cr2 Ni4 A. However, the traditional carburizing is limited by poor microstructure distribution and low rate of carburizing. The rare earth ion implantation is known to help improving the properties of tribology, corrosion resistance and oxidation resistance of metal. In this article, the RE implantation is employed to assist the carburizing. Lanthanum and cerium ion implantations are initially used to assist 12 Cr2 Ni4 A low pressure vacuum carburization.The microstructure, content of retained austenite, hardness, thickness of layer and carbon diffusion were analyzed by optical microscopy(OM), scanning electron microscopy(SEM), X-ray diffraction(XRD) and Rockwell/Vickers hardness tester, respectively. It was shown that lanthanum and cerium implantations can improve structure of the vacuum carburizing layer, and enhance the uniformity of carbon element distribution on the carburized surface. Meanwhile the RE implantation plays a positive role in promoting the surface hardness and carburized rate. The lanthanum element has more significant effect on surface hardness and content of retained austenite than cerium element. The surface hardness of lanthanum element implanted layer was 62.9 HRC with 9.6% content of retained austenite, while the carburizing rate of cerium implanted layer increased by 12.4%. | Meiling Dong Xiufang Cui Yuhui Zhang Guo Jin Caiwen Yue Xin Zhao Zhaobing Cai Binshi Xu | 2017 | Journal of Rare Earths2017，35，11： | 3 |
| 8 | Blockchain-Based Reliable Fog-Cloud Service Solution for IIoT显示文摘Industrial Internet of things(IIoT)deploys a large number of smart devices to obtain industrial data,which will be transmitted to cloud for analysis to improve industrial productivity.The management of large-scale devices is complicated,and it’s also a challenge to choose a high-quality cloud service for data analysis as the number of service with similar functions increases.To address these issues,we propose a reliable fog-cloud service solution with blockchain-based fog-cloud architecture.In fog layer,we build a management blockchain between fog servers and design a management method for industrial devices;In cloud layer,we construct a service blockchain between cloud service providers to form an open'service market'.Quality of service and reputation based matching algorithm and reputation-based consensus algorithm are designed.The simulation results show correctness and efficiency of algorithms,and validate effectiveness of our proposed solution. | DAI Meiling XU Siya SHAO Sujie GUO Shaoyong QIU Xuesong XIONG Ao | 2021 | Chinese Journal of Electronics2021，30，2： | 3 |
| 9 | Nonhematopoietic erythropoietin derivative protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis显示文摘Objective:Carbamylated EPO(CEPO)is a derivative of erythropoietin(EPO)by subjecting it to carbamylation.It does not stimulate erythropoiesis,but effectively protects tissue from injury.The present study was to investigate the effect of CEPO treatment using in vitro models of hypoxia/reoxygenation(H/R).Methods:Cardiomyocytes were exposed to hypoxia(95% N2 and 5% CO2)for 1 hour followed by 4 hours of reoxygenation(95% O2 and 5% CO2).CEPO was administered after hypoxia,just before reoxygenation.The apoptotic cardiomyocytes were determined by flow cytometry.The level of protein was assessed by western blot analysis.Results:CEPO treatment significantly decreased the apoptotic cardiomyocytes by 54.20% compared with H/R group.Western blot analysis showed that CEPO administration increased the level of Bcl-2(an antiapoptotic protein)by 62.22% compared with H/R group.Conclusion:Acute administration of CEPO protected cardiomyocytes from H/R-induced apoptosis.CEPO protected cardiomyocytes with a concomitant upregulation of Bcl-2 after H/R injury. | Xuan Xu Xiaohong Shan Zhijuan Cao Meiling Wu Qi Chen Yuehua Li | 2008 | Journal of Nanjing Medical University2008，22，2： | 2 |
| 10 | Effects of Application of Cassava Alcohol Anaerobic Fermentation Liquid and Mulching of Film on Overwintering and Next Year's Growth and Yield of Banana显示文摘The effects of application of cassava alcohol anaerobic fermentation liquid and mulching of film on overwintering and next year's growth and yield of banana were investigated in this study.The results showed that the application of cassava alcohol anaerobic fermentation liquid with COD concentration of 1 200-2 000 mg/L(900 m^3/hm^2) and mulching of film significantly increased the soil organic matter and available potassium contents in banana orchard,improved the cold resistance of banana seedlings,increased the pseudostem height,pseudostem circumference,green leaf number and chlorophyll content of next year's banana seedlings,brought the flower bud emergence period and harvest period forward,and significantly increased the yield per plant. | Yunchuan MO Yingyi DENG Xu ZHENG Yixin YE Ganhui MO Minzheng WEI Weiliu LI Feng LI Jiechun PAN Chanchan QIN Meiling SU Yao FU | 2016 | Agricultural Science & Technology2016，17，7： | 2 |
| 11 | An investigation on tile fusibility characteristicsoflow-rankcoalsandbiomassmixturcs显示文摘 | Li Fenghai Xu Meiling Wang Tao | 2015 | Fuel2015，158，： | 1 |
| 12 | A multicentre randomised trial comparing weekly paclitaxel<ce:hsp sp='0.25'/>+<ce:hsp sp='0.25'/>S-1 with weekly paclitaxel<ce:hsp sp='0.25'/>+<ce:hsp sp='0.25'/>5-fluorouracil for patients with advanced gastric cancer显示文摘 | Dingzhi Huang Yi Ba Jianping Xiong Nong Xu Zhao Yan Zhixiang Zhuang Zhuang Yu Huiping Wan Yang Zhang Ting Deng Rongsheng Zheng Zengqing Guo Chunhong Hu Meiling Wang Zhonghe Yu Yang Yao Jichang Meng | 2013 | European Journal of Cancer2013，，： | 1 |
| 13 | Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic–ischemic encephalopathy显示文摘 | Xinhua Zhang Qinfen Zhang Wei Li Dekang Nie Weiwei Chen Chunxiang Xu Xin Yi Jinhong Shi Meiling Tian Jianbing Qin Guohua Jin Wenjuan Tu | 2014 | Journal of Neuroscience Research2014，，1： | 1 |
| 14 | Experimental Wave Attenuation Study over Flexible Plants on a Submerged Slope显示文摘Using plants is a kind of environmentally-friendly coastal protection to attenuate wave energy. In this paper, a set of experiments were conducted to investigate the wave attenuation performance using flexible grasses on a submerged slope, and the wave attenuation coefficient for these experiments was calculated for different still water depths, slope and grass configurations. It was found that the slope plays a significant role in wave attenuation. The wave attenuation coefficient increases with increasing relative row number and relative density. For a small relative row number, the two configurations from the slope top to its toe and from the slope toe to its top performed equally to a large extent. For a medium relative row number, the configuration from the slope toe to its top performed more poorly than that from the slope top to its toe; however, it performed better than that from the slope top to its toe for a high relative row number. With a single row of grasses close to the slope top from the slope toe, the wave attenuation coefficient shows double peaks. With increasing grass rows or still water depth, the grass location corresponding to the maximum wave attenuation coefficient is close to the slope top. The dimensional analysis and the least square method were used to derive an empirical equation of the wave attenuation coefficient considering the effect of relative density, the slope, the relative row number and the relative location of the middle row, and the equation was validated to experimental data. | YIN Zegao YANG Xiaoyu XU Yuanzhao DING Meiling LU Haixiang | 2017 | Journal of Ocean University of China2017，16，6： | 1 |
| 15 | Immunochromatographic Assay for Ultrasensitive Detection of Aflatoxiu B1 in Maize by Highly Luminescent Quantum Dot Beads显示文摘 | Meiling Ren Hengyi Xu Xiaolin Huang | 2014 | American Chemical Society2014，6，14： | 1 |
| 16 | The influence of water soluble pentosan on viscoelasticity of gluten显示文摘 | MA Fumin XU Shiying XU Meiling | 2012 | Journal of Food Engineering2012，111，1： | 1 |
| 17 | Energy performance and consumption for biogas heat pump air conditioner显示文摘 | Xu Zhenjun Wu Huaizhi Wu Meiling | 2010 | Energy2010，35，12： | 1 |
| 18 | On the least signless Laplacian eigenvalue ofsome graphs显示文摘 | Yu Guanglong Guo Shuguang Xu Meiling | 2013 | Linear Algebra Appl2013，26，： | 1 |
| 19 | On the least signless Laplacian eigenvalue ofnon-bipartite unicyclic graphs with both given order and diameter 显示文摘 | Guo Shuguang Xu Meiling Yu Guanglong | 2014 | Ars Combin2014，114，： | 1 |
| 20 | Neurodegeneration-associated FUS is a novel regulator of circadian gene expression显示文摘Background:Circadian rhythms are oscillating physiological and behavioral changes governed by an internal molecular clock,and dysfunctions in circadian rhythms have been associated with ageing and various neurodegenerative diseases.However,the evidence directly connecting the neurodegeneration-associated proteins to circadian control at the molecular level remains sparse.Methods:Using meta-analysis,synchronized animals and cell lines,cells and tissues from FUS R521C knock-in rats,we examined the role of FUS in circadian gene expression regulation.Results:We found that FUS,an oscillating expressed nuclear protein implicated in the pathogenesis of amyotrophic lateral sclerosis(ALS)and frontotemporal dementia(FTD),exerted a novel feedback route to regulate circadian gene expression.Nr1d1-encoded core circadian protein REV-ERBαbound the Fus promoter and regulated the expression of Fus.Meanwhile,FUS was in the same complex as PER/CRY,and repressed the expression of E box-containing core circadian genes,such as Per2,by mediating the promoter occupancy of PSF-HDAC1.Remarkably,a common pathogenic mutant FUS(R521C)showed increased binding to PSF,and caused decreased expression of Per2.Conclusions:Therefore,we have demonstrated FUS as a modulator of circadian gene expression,and provided novel mechanistic insights into the mutual influence between circadian control and neurodegeneration-associated proteins. | Xin Jiang Tao Zhang Haifang Wang Tao Wang Meiling Qin Puhua Bao Ruiqi Wang Yuwei Liu Hung-Chun Chang Jun Yan Jin Xu | 2018 | Translational Neurodegeneration2018，7，1： | 1 |
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