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| 1 | Intestinal lysozyme liberates Nod1 ligands from microbes to direct insulin trafficking in pancreatic beta cells显示文摘Long-range communication between intestinal symbiotic bacteria and extra-intestinal organs can occur through circulating bacterial signal molecules, through neural circuits, or through cytokines or hormones from host cells. Here we report that Nod1 ligands derived from intestinal bacteria act as signal molecules and directly modulate insulin trafficking in pancreatic beta cells. The cytosolic peptidoglycan receptor Nod1 and its downstream adapter Rip2 are required for insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, Nod1 and Rip2 localize to insulin vesicles, recruiting Rab1a to direct insulin trafficking through the cytoplasm. Importantly, intestinal lysozyme liberates Nod1 ligands into the circulation, thus enabling long-range communication between intestinal microbes and islets. The intestine-islet crosstalk bridged by Nod1 ligands modulates host glucose tolerance. Our study defines a new type of inter-organ communication based on circulating bacterial signal molecules, which has broad implications for understanding the mutualistic relationship between microbes and host. | Qin Zhang Ying Pan Benhua Zeng Xiaojiao Zheng Haifang Wang Xueying Shen Hui Li Qian Jiang Jiaxu Zhao Zhuo-Xian Meng Pingping Li Zhengjun Chen Hong Wei Zhihua Liu | 2019 | Cell Research2019，29，7： | 11 |
| 2 | Fe_3O_4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration显示文摘Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed. | Xiaoliang Zhang Manli He Jia-Hui Liu Rong Liao Lianqin Zhao Jingru Xie Ruijue Wang Sheng-Tao Yang Haifang Wang Yuanfang Liu | 2014 | Chinese Science Bulletin2014，59，27： | 8 |
| 3 | Directly patterned substrate-free plasmonic “nanograter” structures with unusual Fano resonances显示文摘The application of three-dimensional(3D)plasmonic nanostructures as metamaterials(MMs),nano-antennas,and other devices faces challenges in producing metallic nanostructures with easily definable orientations,sophisticated shapes,and smooth surfaces that are operational in the optical regime and beyond.Here,we demonstrate that complex 3D nanostructures can be readily achieved with focused-ion-beam irradiation-induced folding and examine the optical characteristics of plasmonic“nanograter”structures that are composed of free-standing Au films.These 3D nanostructures exhibit interesting 3D hybridization in current flows and exhibit unusual and well-scalable Fano resonances at wavelengths ranging from 1.6 to 6.4 mm.Upon the introduction of liquids of various refractive indices to the structures,a strong dependence of the Fano resonance is observed,with spectral sensitivities of 1400 nm and 2040 nm per refractive index unit under figures of merit of 35.0 and 12.5,respectively,for low-order and high-order resonance in the near-infrared region.This work indicates the exciting,increasing relevance of similarly constructed 3D free-standing nanostructures in the research and development of photonics and MMs. | Ajuan Cui Zhe Liu Jiafang Li Tiehan H Shen Xiaoxiang Xia Zhiyuan Li Zhijie Gong Hongqiang Li Benli Wang Junjie Li Haifang Yang Wuxia Li Changzhi Gu | 2015 | Light(Science & Applications)2015，4，1： | 8 |
| 4 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 5 | The EGFR-P38 MAPK axis up-regulates PD-L1 through miR-675-5p and down-regulates HLA-ABC via hexokinase-2 in hepatocellular carcinoma cells显示文摘Background:Immunotherapy has been shown to be a promising strategy against human cancers.A better understanding of the immune regulation in hepatocellular carcinoma(HCC)could help the development of immunotherapy against HCC.The epidermal growth factor receptor(EGFR)signaling is frequently activated in HCC and plays important roles in tumorigenesis.However,its role in HCC immunity is still largely unknown.This study aimed to investigate the impact of EGFR signaling on programmed death-ligand 1(PD-L1)and human leukocyte antigen class-I(HLA-I)expression in HCC cells and its underlying mechanisms.Methods:The expression of phosphorylated EGFR(p-EGFR),PD-L1,and HLAI(HLA-ABC)in HCC specimens was detected by immunohistochemistry,and their correlations were analyzed.PD-L1 and HLA-ABC expression in EGFRactivated HCC cells were detected by quantitative real-time PCR,Western blotting,and flow cytometry,and T cell-mediated lysis was performed to test the immunosuppressive effects of PD-L1 and HLA-ABC alterations in HCC cells.Furthermore,the underlying mechanisms of EGFR activation-induced PD-L1 up-regulation and HLA-ABC down-regulation were explored by animal experiments,luciferase reporter assay,and gene gain-and loss-of-function studies.Results:p-EGFR was positively correlated with PD-L1 and negatively correlated with HLA-ABC expression in HCCs.EGFR activation by its ligand EGF up-regulated PD-L1 and down-regulated HLA-ABC in HCC cells,which was functionally important and could be abolished by the EGFR inhibitor,gefitinib,both in vitro and in vivo.Mechanistically,enhanced P38 mitogenactivated protein kinase(MAPK)activation down-regulated microRNA-675-5p(miR-675-5p)and up-regulated glycolysis-related enzyme hexokinase 2(HK2);miR-675-5p down-regulation enhanced the stability of PD-L1 mRNA probably via the 3’-untranslated region(3’-UTR)of PD-L1 and thereby caused PD-L1 accumulation,and HK2 up-regulation enhanced aerobic glycolysis and mediated a decrease in HLA-ABC.Conclusions:The EGFR-P38 MAPK axis could up-regulate PD-L1 through miR-675-5p and down-regulate HLA-ABC via HK2 in HCC cells.Our study reveals a novel signaling network that may cause immune suppression in HCC and suggests that EGFR signaling can be targeted for HCC immunotherapy. | Zongcai Liu Fen Ning Yanna Cai Huiying Sheng Ruidan Zheng Xi Yin Zhikun Lu Ling Su Xiaodan Chen Chunhua Zeng Haifang Wang Li Liu | 2021 | Cancer Communications2021，41，1： | 5 |
| 6 | PEGylation of Double-Walled Carbon Nanotubes for Increasing Their Solubility in Water显示文摘Polyethylene glycol(PEG)functionalized double-walled carbon nanotubes(DWNTs)have been synthesized by a[2+1]cycloaddition reaction and characterized by transmission electron microscopy,atomic force microscopy,Raman spectroscopy,thermal gravimetric analysis,and UV-visible spectroscopy.Functionalization affords a large increase in the aqueous solubility of DWNTs.The saturated concentrations of DWNTs functionalized with diazido-terminated PEG800(with a molecular weight of 800)and azido-terminated PEG750 monomethylether(with a molecular weight of 750)are very similar-0.36 and 0.37 mg/mL(DWNTs equivalent concentration),respectively. | Haiyu Nie Wei Guo Yuan Yuan Zengpei Dou Zujin Shi Zhen Liu Haifang Wang Yuanfang Liu | 2010 | Nano Research2010，3，2： | 4 |
| 7 | Interaction of multi-walled carbon nanotubes and zinc ions enhances cytotoxicity of zinc ions显示文摘More and more nanomaterials enter the environment along with their production, application and deposal. They may alter the biological effect of pollutants already existing in the real environment by different interactions. Therefore efforts should also be paid to investigate the combined toxicity of nanomaterials and pollutants. Herein, we studied the combined toxicity of oxidized multi-walled carbon nanotubes(O-MWCNTs) and zinc ions on cells. It is found that cytotoxicity of the combined O-MWCNTs and zinc ions elevates significantly, compared with O-MWCNTs or zinc ions alone. This result comes from the assays of cell morphology, cell viability and proliferation, cell membrane integrity, mitochondrial membrane potential and cell apoptosis. Mechanism studies indicate that O-MWCNTs absorb zinc ions and form slight aggregation. These enhance remarkably the cellular uptake of O-MWCNTs, and thus induce the death of cells by bringing in more zinc ions into cells. Our study indicates that the existence of nanomaterials could change the bioconsequence of other pollutants and emphasizes the importance of the combined toxicity research in the presence of nanomaterials. | Lin Wang Jia-Hui Liu Zheng-Mei Song Yi-Xin Yang Aoneng Cao Yuanfang Liu Haifang Wang | 2016 | Science China Chemistry2016，59，7： | 2 |
| 8 | Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei显示文摘Titania nanotubes(TiO2-NTs)are a potential drug vehicle for use in nanomedicine.To this end,a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out.TiO2-NTs were first conjugated with a fl uorescent label,fl uorescein isothiocyanate(FITC).FITC-conjugated titania nanotubes(FITC-TiO2-NTs)internalized in mouse neural stem cells(NSCs,line C17.2)can be directly imaged by confocal microscopy.The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells.After co-incubation with cells for 24 h,FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca.More interestingly,the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h.Atomic force microscopy(AFM)and transmission electron microscopy(TEM)were also employed in tracking the nanotubes in the cell.These results will be of benefit in future studies of TiO2-NTs for use as a drug vehicle,particularly for DNA-targeting drugs. | Yanli Wang Jia Wang Xiaoyong Deng Jiao Wang Haifang Wang Minghong Wu Zheng Jiao Yuanfang Liu | 2009 | Nano Research2009，2，7： | 2 |
| 9 | Reactive oxygen species and nitric oxide induce senescence of rudimentary leaves and the expression profiles of the related genes in Litchi chinensis显示文摘Litchi is one of the most important subtropical evergreen fruit trees in southern Asia.Previous studies indicated that high-temperature conditions encourage growth of rudimentary leaves in panicles and suppress flowering.We have demonstrated that methyl viologen dichloride hydrate(MV)and sodium nitroprusside(SNP)promoted flowering in litchi partially by inhibiting the growth of rudimentary leaves via reactive oxygen species(ROS)and nitric oxide(NO).In the present study,we examined the microstructure and ultrastructure,programmed cell death(PCD)ratio,nuclei morphology of the rudimentary leaves,and the expression of senescence-related genes after the treatment with ROS or NO.The results showed that chromatins of the ROS-or NO-treated cells in the rudimentary leaves were condensed.Fusion of the cytoplasm-digesting vesicles with the vacuole and degradation of cytoplasm forming scattered debris were found in those of the treated cells.Treatment with ROS or NO increased the cell PCD ratio.Morphology of the nuclei stained by propidium iodide(PI)showed that nuclei shape became irregular after the ROS or NO treatment.Further,the expression levels of LcRboh,LcMC-1-like,and LcPirin were higher in the ROS-and NO-treated rudimentary leaves than those in the control ones,suggesting that these genes may be involved in the ROS and NO-induced senescence and abscission of the rudimentary leaves in litchi.Our results suggested that ROS and NO play an important role in inducing the senescence of the rudimentary leaves,and ROS-and NO-induced PCD may be involved in the regulation of the rudimentary leaf growth in litchi. | Haifang Yang Hye-Ji Kim Houbin Chen Yong Lu Xingyu Lu Congcong Wang Biyan Zhou | 2018 | Horticulture Research2018，5，1： | 2 |
| 10 | Stable isotope labeling of nanomaterials for biosafety evaluation and drug development显示文摘Quantitative information,such as environmental migration,absorption,biodistribution,biotransformation,and elimination,is fundamental and essential for the nanosafety evaluations of nanomaterials.Due to the complexity of biological and environmental systems,it is challenging to develop quantitative approaches and tools that could characterize intrinsic behaviors of nanomaterials in the organisms.The isotopic tracers are ideal candidates to tune the physical properties of nanomaterials while preserving their chemical properties.In this review article,we summarized the stable isotope labeling methods of nanomaterials for evaluating their environmental and biological effects.The skeleton labeling protocols of carbon nanomaterials and metal/metal oxide nanoparticles were introduced.The advantages and disadvantages of stable isotope labeling were discussed in comparison with other quantitative methods for nanomaterials.The quantitative information of nanomaterials in environmental and biological systems was summarized along with the biosafety data.The benefits for drug development of nanomedicine were analyzed based on the targeting effects,persistent accumulation,and safety.Finally,the challenges and future perspectives of stable isotope labeling in nanoscience and nanotechnology were discussed. | Xue-Ling Chang Lingyun Chen Boning Liu Sheng-Tao Yang Haifang Wang Aoneng Cao Chunying Chen | 2022 | Chinese Chemical Letters2022，33，7： | 2 |
| 11 | Applications of ^(14)C-AMS in biomedical sciences (Bio-^(14)C-AMS)显示文摘Accelerator mass spectrometry (AMS) is an ultrasensitive measure for tracing 14C labeled molecules in vivo or detecting the biomarker for assessment of carcino-genesis. In this review, basic principles, wide applications and new progresses of 14C-bio-AMS are presented. It has been a new advanced tool for measuring the adduction of biologcial molecules with xenobiotics, including carcinogens, drugs, agrochemicals, nicotine, etc. The successful applications have proven the effectiveness of AMS to assessing cancer risk, screening drug toxicity and studying nutrients uptake. In particular, AMS is characterized by measuring xenobiotics at very low dose levels relevant to human environmental exposure. It is sensitive and precise to an attomole (10~18 mole) or less of 14C per mg carbon. Although it has some shortcomings, undoubtedly, AMS possesses an evident merit of high sensitivity and will have widespread applications in the biomedical sciences. | WANG Haifang, SUN Hongfang & LIU YuanfangDepartment of Technical Physics, Peking University, Beijing 100871,ChinaCorrespondence should be addressed to Liu Yuangfang (e-mail: yliu@pku.edu.cn) | 2001 | Chinese Science Bulletin2001，46，7： | 1 |
| 12 | MicroRNA-101 inhibits proliferation of pulmonary microvascular endothelialcells in a rat model of hepatopulmonary syndrome by targeting the JAK2/STAT3 signalingpathway显示文摘 | Liguo Wang Liwei Zhuang Haifang Rong Yuening Guo Xiaohua Ling Ruifeng Wang Xin Yu Wei Zhang | 2015 | Molecular Medicine Reports2015，，6： | 1 |
| 13 | Isolation and purification of salvianolic acid A and salvianolic acid B from Salvia miltiorrhiza by high-speed countercurrent chromatography and comparison of their antioxidant activity 显示文摘 | SUN Yinshi ZHU Haifang WANG Jianhua | 2009 | Journal of Chromatography B2009，877，89： | 1 |
| 14 | A glucose biosensorbased on Prussian blue/chitosan hybrid film显示文摘 | WANG Xueying GU Haifang YIN Fan | 2009 | Biosensors andbioelectronics2009，24，5： | 1 |
| 15 | Biodistribution of carbon single-wall nanotubes in mice显示文摘 | Wang Haifang Wang J Deng XY | 2004 | J Nanosci Nanotecb2004，4，： | 1 |
| 16 | Association of two porcine reproductive and respiratory syndrome virus (PRRSV) receptor genes, CD163 and SN with immune traits显示文摘 | Fengli Wang Haifang Qiu Qingde Zhang Zhongzhen Peng Bang Liu | 2012 | Molecular Biology Reports2012，，4： | 1 |
| 17 | Neurotoxicity of low-dose repeatedly intranasal instillation of nano- and submicron-sized ferric oxide particles in mice显示文摘 | Bing Wang Weiyue Feng Motao Zhu Yun Wang Meng Wang Yiqun Gu Hong Ouyang Huajian Wang Ming Li Yuliang Zhao Zhifang Chai Haifang Wang | 2009 | Journal of Nanoparticle Research2009，，1： | 1 |
| 18 | A unified model for the analysis of facts devices in damping power system oscillations-part iii:unified power flow controller显示文摘 | Wang Haifang | 2000 | IEEE Transaction on Power Deliver2000，15，3： | 1 |
| 19 | Dynamic self-assembly synthesis and controlled release as drug vehicles of porous hollow silica nanoparticles显示文摘 | Shun Tan Qiuxia Wu Jia Wang Yanli Wang Xiaolei Liu Keke Sui Xiaoyong Deng Haifang Wang Minghong Wu | 2011 | Microporous and Mesoporous Materials2011，，2： | 1 |
| 20 | Atomic Force Microscopy study of the structural effects induced by echinomycin binding to DNA显示文摘 | Yolanda D Tseng Haifang Ge Xiuzhu Wang | 2005 | J Mol Bid2005，345，： | 1 |
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