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| 1 | Construction of an electronic physical map of Magnaporthe oryzae using genomic position-ready SSR markers显示文摘Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat (SSR)-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were locatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr (avirulence) genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolu- tionally related pathogens. | FENG ShuJie MA JunHong LIN Fei WANG Ling PAN QingHua | 2007 | Chinese Science Bulletin2007，52，24： | 5 |
| 2 | Regional Soil Mapping Using Multi-Grade Representative Sampling and a Fuzzy Membership-Based Mapping Approach显示文摘High-resolution and detailed regional soil spatial distribution information is increasingly needed for ecological modeling and land resource management. For areas with no point data, regional soil mapping includes two steps: soil sampling and soil mapping. Because sampling over a large area is costly, efficient sampling strategies are required. A multi-grade representative sampling strategy, which designs a small number of representative samples with different representative grades to depict soil spatial variations at different scales,could be a potentially efficient sampling strategy for regional soil mapping. Additionally, a suitable soil mapping approach is needed to map regional soil variations based on a small number of samples. In this study, the multi-grade representative sampling strategy was applied and a fuzzy membership-weighted soil mapping approach was developed to map soil sand percentage and soil organic carbon(SOC) at 0–20 and 20–40 cm depths in a study area of 5 900 km2 in Anhui Province of China. First, geographical sub-areas were delineated using a parent lithology data layer. Next, fuzzy c-means clustering was applied to two climate and four terrain variables in each stratum. The clustering results(environmental cluster chains) were used to locate representative samples. Evaluations based on an independent validation sample set showed that the addition of samples with lower representativeness generally led to a decrease of root mean square error(RMSE). The declining rates of RMSE with the addition of samples slowed down for 20–40 cm depth, but fluctuated for 0–20 cm depth. The predicted SOC maps based on the representative samples exhibited higher accuracy, especially for soil depth 20–40 cm, as compared to those based on legacy soil data. Multi-grade representative sampling could be an effective sampling strategy at a regional scale. This sampling strategy, combined with the fuzzy membership-based mapping approach, could be an optional effective framework for regional soil property mapping. A more detailed and accurate soil parent material map and the addition of environmental variables representing human activities would improve mapping accuracy. | YANG Lin A-Xing ZHU ZHAO Yuguo LI Decheng ZHANG Ganlin ZHANG Shujie Lawrence E. BAND | 2017 | Pedosphere2017，27，2： | 5 |
| 3 | Genetic and physical mapping of AvrPi7, a novel avirulence gene of Magnaporthe oryzae using physical position-ready markers显示文摘Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating crop diseases worldwide. The avirulence gene corresponding to rice blast resistance gene Pi7 in field isolate CHL346 was inherited as a single gene, designated AvrPi7, in a segregating population consisting of 189 ascospore progenies derived from a cross between field isolates CHL346 and CHL42. In order to determine the chromosomal location of the AvrPi7 locus, a total of 121 simple sequence repeat (SSR) markers were developed based on the whole-genome sequence of reference isolate 70-15 of M. oryzae. Linkage analysis of the locus with these SSR markers showed that eight SSR markers on chromosome 1 were linked to the locus, among which the closest flanking markers MS1-9 and MS1-15 were 3.2 and 16.4 cM from the locus, respectively. For fine mapping, additional PCR-based makers including eight SSR markers and three candidate avirulence gene (CAG) markers were developed in the region flanking both markers. The AvrPi7 locus was genetically delimited within a 1.6-cM region flanked by markers MS1-21 and MS1-22, and co-segregated with the marker CAG2. To construct a physical map of the AvrPi7 locus, molecular markers linked to the Avr gene were mapped on the supercontigs of the ref-erence isolate 70-15 through bioinformation analysis (BIA). Consequently, the AvrPi7 locus was delim-ited to a 75-kb interval flanked by markers MS1-21 and MS1-22 based on the reference sequence. Merodiploids observed in this study are also discussed. | FENG ShuJie WANG Ling MA JunHong LIN Fei PAN QingHua | 2007 | Chinese Science Bulletin2007，52，7： | 5 |
| 4 | Mapping Detailed Soil Property Using Small Scale Soil Type Maps and Sparse Typical Samples显示文摘Soil type maps at the scale of 1︰1 000 000 are used extensively to provide soil spatial distribution information for soil erosion assessment and watershed management models in China.However,the soil property maps produced through conventional direct linking method usually suffer low accuracy as well as the lack of spatial details within a soil type polygon.This paper presents an effective method to produce detailed soil property map based on representative samples which were extracted from each polygon on the 1︰1000 000 soil type map.The representative sample of each polygon is defined as the location that can represent the largest area within the polygon.The representativeness of a candidate sample is determined by calculating the soil-forming environment condition similarities between the sample and other locations.Once the representative sample of each polygon has been chosen,the property values of the existing typical samples are assigned to the corresponding representative samples with the same soil type.Finally,based on these representative samples,the detailed soil property map could be produced by using existing digital soil mapping methods.The case study in XuanCheng City,Anhui Province of China,demonstrated the proposed method could produce soil property map at a higher level of spatial details and accuracy:1)The soil organic matter(SOM)map produced based on the representative samples can not only depict the detailed spatial distribution of SOM within a soil type polygon but also largely reduce the abrupt change of soil property at the boundaries of two adjacent polygons.2)The Root Mean Squared Error(RMSE)of the SOM map based on the representative samples is1.61,and it is 1.37 for the SOM map produced by using conventional direct linking method.Therefore,the proposed method is an effective approach to produce spatial detailed soil property map with higher accuracy for environment simulation models. | ZHANG Shujie ZHU Axing LIU Wenliang LIU Jing YANG Lin | 2013 | Chinese Geographical Science2013，23，6： | 2 |
| 5 | Boosted photoreduction of diluted CO_(2) through oxygen vacancy engineering in NiO nanoplatelets显示文摘Converting carbon dioxide(CO_(2))to diverse value-added products through photocatalysis can validly alleviate the critical issues of greenhouse effect and energy shortages simultaneously.In particular,based on practical considerations,exploring novel catalysts to achieve efficient photoreduction of diluted CO_(2) is necessary and urgent.However,this process is extremely challenging owing to the disturbance of competitive adsorption at low CO_(2) concentration.Herein,we delicately synthesize oxygen vacancy-laden NiO nanoplatelets(r-NiO)via calcination under Ar protection to reduce diluted CO_(2) through visible light irradiation(>400 nm)assisted by a Ru-based photosensitizer.Benefitting from the strongly CO_(2) adsorption energy of oxygen vacancies,which was confirmed by density functional calculations,the r-NiO catalysts exhibit higher activity and selectivity(6.28×10^(3)µmol·h^(−1)·g^(−1);82.11%)for diluted CO_(2)-to-CO conversion than that of the normal NiO(3.94×10^(3)µmol·h^(−1)·g^(−1);65.26%).Besides,the presence of oxygen vacancies can also promote the separation of electron-hole pairs.Our research demonstrates that oxygen vacancies could act as promising candidates for photocatalytic CO_(2) reduction,offering fundamental guidance for the actual photoreduction of diluted CO_(2) in the future. | Weiyi Chen Xueming Liu Bin Han Shujie Liang Hong Deng Zhang Lin | 2021 | Nano Research2021，14，3： | 2 |
| 6 | Kilometers Long Graphene-Coated Optical Fibers for Fast Thermal Sensing显示文摘The combination of optical fiber with graphene has greatly expanded the application regimes of fiber optics,from dynamic optical control and ultrafast pulse generation to high precision sensing.However,limited by fabrication,previous graphene-fiber samples are typically limited in the micrometer to centimeter scale,which cannot take the inherent advantage of optical fibers—longdistance optical transmission.Here,we demonstrate kilometers long graphene-coated optical fiber(GCF)based on industrial graphene nanosheets and coating technique.The GCF shows unusually high thermal diffusivity of 24.99 mm^(2) s^(-1) in the axial direction,measured by a thermal imager directly.This enables rapid thermooptical response both in optical fiber Bragg grating sensors at one point(18-fold faster than conventional fiber)and in long-distance distributed fiber sensing systems based on backward Rayleigh scattering in optical fiber(15-fold faster than conventional fiber).This work realizes the industrial-level graphene-fiber production and provides a novel platform for two-dimensional material-based optical fiber sensing applications. | Yiyong Guo Bing Han Junting Du Shanshan Cao Hua Gao Ning An Yiwei Li Shujie An Zengling Ran Yue Lin Wencai Ren Yunjiang Rao Baicheng Yao | 2021 | Research2021，，1： | 2 |
| 7 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 8 | Economic growth and strnctural changes in employment and investments in China 1985 ~ 94显示文摘 | Aying Lin Shujie Yao Zongyi Zhang | 1999 | Economic Planning1999，32，： | 1 |
| 9 | Prognostic Significance of MTOR Pathway Component Expression in Neuroendocrine Tumors显示文摘 | Zhi Rong Qian Monica Ter-Minassian Jennifer A. Chan Yu Imamura Susanne M. Hooshmand Aya Kuchiba Teppei Morikawa Lauren K. Brais Anastassia Daskalova Rachel Heafield Xihong Lin David C. Christiani Charles S. Fuchs Shuji Ogino Matthew H. Kulke | 2013 | Journal of Clinical Oncology2013，，27： | 1 |
| 10 | A multifunctional neuromodulation platform utilizing Schwann cell-derived exosomes orchestrates bone microenvironment via immunomodulation, angiogenesis and osteogenesis显示文摘Recent evidence highlights multifaceted biological needs to recapitulate the bone microenvironment for bone regeneration.Neurotization has great potential for realizing multi-system modulations in bone tissue engineering(BTE).However,a neural strategy involving all the key bone repair steps temporally has not yet been reported.In this study,we reported the neural tissue engineering hydrogel-encapsulated Schwann cell-derived exosomes(SC Exo).This sustained-release SC Exo system prominently enhanced bone regeneration by promoting innervation,immunoregulation,vascularization,and osteogenesis in vivo.Moreover,the in vitro results further confirmed that this system significantly induced M2 polarization of macrophages,tube formation of HUVECs,and BMSCs osteogenic differentiation.Furthermore,BMSCs osteogenesis was promoted by upregulating the TGF-β1/SMAD2/3 signaling pathway.In summary,a novel cell-free and easily prepared SC Exo neural engineering was successfully developed to promote bone regeneration by orchestrating the entire bone healing microenvironment,which may provide a new strategy for tissue engineering and clinical treatment of bone defects. | Zhichao Hao Lin Ren Zhen Zhang Zaiwu Yang Shujie Wu Gen Liu Bin Cheng Jun Wu Juan Xia | 2023 | Bioactive Materials2023，，5： | 1 |
| 11 | miR-124 Represses ROCK1 Expression to Promote Neurite Elongation Through Activation of the PI3K/Akt Signal Pathway显示文摘 | Xi Gu Siying Meng Shuhu Liu Chunhong Jia Yingying Fang Shuji Li Congcong Fu Qiancheng Song Lifang Lin Xuemin Wang | 2014 | Journal of Molecular Neuroscience2014，，1： | 1 |
| 12 | Multi-attribute group decision-making method with linguistic q-rung orthopair fuzzy information based on bi-direction Choquet integral显示文摘Purpose-The purpose of this paper is to develop the linguistic q-rung orthopair fuzzy set(LqROFS)information VIKOR method based on the bi-direction Choquet integral(BDCI),taking into account the correlation between information.The method can enrich the existing studies related to LqROFS information and better solve the problem of MAGDM problem.Design/methodology/approach-Since applying Choquet integral(CI)depict information interaction is a common operation in MAGDM.However,the traditional CI has some limitations.The unidirectional alignment may affect the MAGDM results.Therefore,a LqROFS-VIKOR method based on BDCI is proposed,where BDCI is used to aggregate the decision matrix.Furthermore,it is not reasonable to apply exact numbers to express the similarity between two qualitative data.Then a new method of defining similarity using linguistics is proposed.The similarity is used to calculate attribute weights.Findings-The validity and potential application of MAGMD method with linguistic q-rung orthopair fuzzy information based on BDCI are demonstrated in a numerical examples study.Originality/value-According to the study of available literature,the current research on LqROFS is incomplete.The existing studies of both similarity and aggregate operators have certain shortcomings.The definition of similarity proposed in this paper is more in line with reality.And compared with the existing methods,the BDCI-based aggregate operator can describe the interaction between information more reasonably.Based on this VIKOR method based on BDCI under the LqROFS environment can better select the alternative. | Ling Weng Jian Lin Shujie Lv | 2023 | International Journal of Intelligent Computing and Cybernetics2023，16，2： | 0 |
| 13 | CALCULATION OF THE SECOND-ORDER OPTICAL SUSCEPTIBILITIES OF SOME BENZENE DERIVATIVE MOLECULES AND CRYSTALS显示文摘The microscopic and macroscopic second-order optical susceptibilities of ten benzene derivative molecules and three crystals were calculated and discussed,basing on the nonlinear optical active group theory. | LIN Shujie CHEN Chuangtian | 1990 | Chinese Physics Letters1990，7，6： | 0 |
| 14 | Nanozymes for biomedical applications in orthopaedics显示文摘As the next generation of artificial enzymes,nanozymes have attracted increasing attention in biomedical applications due to their multienzyme-like characteristics,multifunctionalities,low cost,and high stability.By taking advantage of their diverse activities,a growing number of nanozyme-mediated therapeutic strategies have been developed for various diseases.Herein,we provide a brief review of the representative studies of nanozymes,especially in orthopaedic diseases over the past decade,which include arthritis,osteoporosis,bone regeneration,bacteria-associated infections,and osteosarcoma.Moreover,the future potential applications and some major challenges are also discussed.This review would not only provide some instructive views of nanozymes but also promote the development of enzyme-mimetic strategies in orthopaedics. | Anqi Lin Shujie Liu Hui Wei | 2023 | Particuology2023，，5： | 0 |
| 15 | miR-424(322)-5p targets Ezh1 to inhibit the proliferation and differentiation of myoblasts显示文摘The proliferation and differentiation of myoblasts are considered the key biological processes in muscle development and muscle-related diseases,in which the miRNAs involved remain incompletely understood.Previous research reported that miR-424(322)-5p is highly expressed in mouse skeletal muscle.Therefore,C2C12 cells are used as a model to clarify the effect of miR-424(322)-5p on the proliferation and differentiation of myoblasts.The data show that miR-424(322)-5p exhibits a decreasing trend upon myogenic differentiation.Overexpression of miR-424(322)-5p inhibits the proliferation of myoblasts,manifested by downregulation of proliferation marker genes(CCNB1,CCND2,and CDK4),decreased percentage of EdU^(+)cells,and reduced cell viability.In contrast,these phenotypes are promoted in myoblasts treated with an inhibitor of miR-424(322)-5p.Interestingly,its gain of function inhibits the expression of myogenic regulators,including MyoD,MyoG,MyHC,and Myf5.Additionally,immunofluorescence staining of MyHC and MyoD shows that overexpression of miR-424(322)-5p reduces the number of myotubes and decreases the myotube fusion index.Consistently,inhibition of its function mediated by an inhibitor promotes the expressions of myogenic markers and myotube fusion.Mechanistically,gene prediction and dual-luciferase reporter experiments confirm that enhancer of zeste homolog 1(Ezh1)is one of the targets of miR-424(322)-5p.Furthermore,knockdown of Ezh1 inhibits the proliferation and differentiation of myoblasts.Compared with NC and inhibitor treatment,inhibitor+si-EZH1 treatment rescues the phenotypes of proliferation and differentiation mediated by the miR-424(322)-5p inhibitor.Taken together,these data indicate that miR-424(322)-5p targets Ezh1 to negatively regulate the proliferation and differentiation of myoblasts. | Yongqi Yue Xinxin Feng Yige Jia Shujie Luo Menglei Jiang Jiandan Luo Yonglin Hua Jiyue Zhang Yaqiu Lin Jian Li Yan Xiong | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 0 |
| 16 | Characterization strategy of polymeric transition metal species transformation for high-purity metal recovery显示文摘The aqueous metal species with similar chemical properties are usually extracted together,limiting deep separation for high-purity metal.However,rare attention has been paid to metal speciation characterization and transformation during separation.Herein,the hydrolysis evolution of polymeric metal species was investigated systematically by electrospray ionization time-of-flight mass spectrometry(ESI-TOF-MS).The transformation evolutions were visualized with respect to characteristic vanadium species(V_(1),V_(2),V_(3),V_(4) and V_(10)),chromium species(Cr_(1) and Cr_(2)),tungsten(W_(1),W_(2),W_(4),W_(6) and W_(10))and molybdenum(Mo_(1),Mo_(2) and Mo_(4))species.The key characteristics(such as specfic pHs and concentrations)for speciation variation were revealed.The polymerization behavior of several transition metals can be semiquantitative characterized by this strategy.The sufficient speciation transformation provides a solid base for metal speciation chemistry,and guides further development of high-purity metal recovery. | Jiawei Wen Yan Wu Shujie Lin Pengge Ning | 2021 | Green Chemical Engineering2021，2，3： | 0 |
| 17 | Poly(L-lactide)-grafted Bioglass/Poly(lactide-co-glycolide) Scaffolds with Supercritical CO2 Foaming Reprocessing for Bone Tissue Engineering显示文摘 | DONG Shujun WANG Lin LI Qiushi CHEN Xuesi LIU Shujie ZHOU Yanmin | 2017 | Chemical Research in Chinese Universities2017，33，3： | 0 |
| 18 | Lattice-strained nanotubes facilitate efficient natural sunlight-driven CO_(2) photoreduction显示文摘Photocatalytic reduction of CO_(2) holds tremendous promise for alleviating the energy crisis.Despite the progress that has been,made,there are still some challenges to overcome,such as the realization under real sunlight rather than the simulation condition.In this work,ultrathin Ni_(2)(OH)(PO_(4))nanotubes(NTs)prepared through hydrothermal route are applied as a novel catalyst for photocatalytic reduction of CO_(2) under real sunlight.The prepared Ni_(2)(OH)(PO_(4))NTs exhibit a 11.3 μmol·h^(-1) CO production rate with,96.1% CO selectivity.Interestingly,Ni_(2)(OH)(PO_(4))NTs have a positive impact on the facilitation of photoreduction in diluted CO_(2).Notably,when the system is performed under real sunlight,Ni_(2)(OH)(PO_(4))NTs afford an accumulated CO of ca.26.8 nmol with,96.9% CO selectivity,exceeding most previous inorganic catalysts under simulated irradiation in the laboratory.Our experimental results demonstrate that the multisynergetic effects induced by surface-OH and the lattice strain serve as highly active sites for CO_(2) molecular adsorption and activation as well as electron transfer,hence enhancing photoreduction activity.Therefore,this work provides experimental basis that CO_(2) photocatalysis can be put into practical use. | Shujie Liang Xueming Liu Zuqi Zhong Bin Han Xiaohui Zhong Weiyi Chen Kainan Song Hong Deng Zhang Lin | 2021 | Nano Research2021，14，8： | 0 |
| 19 | Bifidobacterium adolescentis orchestrates CD143^(+)cancer-associated fibroblasts to suppress colorectal tumorigenesis by Wnt signaling-regulated GAS1显示文摘Background The interplay between gut microbiota and tumor microenvironment(TME)in the pathogenesis of colorectal cancer(CRC)is not well explored.Here,we elucidated the functional role of Bifidobacterium adolescentis(B.a)on CRC and investigated its possible mechanism on the manipulation of cancer-associated fibroblasts(CAFs)in CRC.Methods Different CRC animal models and various cell line models were established to explore the function of B.a on CRC.The single-cell RNA sequencing(scRNA-seq)or flow cytometry was used to detect the cell subsets in the TME of CRC.Western blot,quantitative real-time polymerase chain reaction(qRT-PCR),or immunofluorescence staining were performed to examine the activation of Wnt signaling and growth arrest specific 1(GAS1)on CD143+CAFs.Chromatin immunoprecipitation quantitative real-time PCR(CHIP-qPCR)was performed to investigate the regulation of transcription factor 4(TCF4)on GAS1.Multi-immunofluorescence assay examined the expression level of CD143 and GAS1 on tissue microarray.Results We found that B.a abundance was significantly reduced in CRC patients from two independent cohorts and the bacteria database of GMrepo.Supplementation with B.a suppressed ApcMin/+spontaneous or AOM/DSS-induced tumorigenesis in mice.scRNA-seq revealed that B.a facilitated a subset of CD143+CAFs by inhibiting the infiltration of Th2 cells,while promoting the TNF-alpha+B cells in TME.CD143+CAFs highly expressed GAS1 and exhibited tumor suppressive effect.Mechanistically,GAS1 was activated by the Wnt/β-catenin signaling in CD143+CAFs.B.a abundance was correlated with the expression level of CD143 and GAS1.The level of CD143+CAFs predicted the better survival outcome in CRC patients.Conclusions These results highlighted that B.a induced a new subset of CD143+CAFs by Wnt signaling-regulated GAS1 to suppress tumorigenesis and provided a novel therapeutic target for probiotic-based modulation of TME in CRC. | Shujie Chen Lina Fan Yifeng Lin Yadong Qi Chaochao Xu Qiwei Ge Ying Zhang Qiwen Wang Dingjiacheng Jia Lan Wang Jianmin Si Liangjing Wang | 2023 | Cancer Communications2023，43，9： | 0 |
| 20 | Dynamic changes in butyrate levels regulate satellite cell homeostasis by preventing spontaneous activation during aging显示文摘The gut microbiota plays a pivotal role in systemic metabolic processes and in particular functions,such as developing and preserving the skeletal muscle system.However,the interplay between gut microbiota/metabolites and the regulation of satellite cell(SC)homeostasis,particularly during aging,remains elusive.We propose that gut microbiota and its metabolites modulate SC physiology and homeostasis throughout skeletal muscle development,regeneration,and aging process.Our investigation reveals that microbial dysbiosis manipulated by either antibiotic treatment or fecal microbiota transplantation from aged to adult mice,leads to the activation of SCs or a significant reduction in the total number.Furthermore,employing multi-omics(e.g.,RNA-seq,16S r RNA gene sequencing,and metabolomics)and bioinformatic analysis,we demonstrate that the reduced butyrate levels,alongside the gut microbial dysbiosis,could be the primary factor contributing to the reduction in the number of SCs and subsequent impairments during skeletal muscle aging.Meanwhile,butyrate supplementation can mitigate the antibiotics-induced SC activation irrespective of gut microbiota,potentially by inhibiting the proliferation and differentiation of SCs/myoblasts.The butyrate effect is likely facilitated through the monocarboxylate transporter 1(Mct1),a lactate transporter enriched on membranes of SCs and myoblasts.As a result,butyrate could serve as an alternative strategy to enhance SC homeostasis and function during skeletal muscle aging.Our findings shed light on the potential application of microbial metabolites in maintaining SC homeostasis and preventing skeletal muscle aging. | Shujie Chen Liujing Huang Bingdong Liu Huimin Duan Ze Li Yifan Liu Hu Li Xiang Fu Jingchao Lin Yinlan Xu Li Liu Dan Wan Yulong Yin Liwei Xie | 2024 | Science China(Life Sciences)2024，67，4： | 0 |
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