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| 1 | Additive manufacturing of hydroxyapatite bioceramic scaffolds: Dispersion, digital light processing, sintering, mechanical properties, and biocompatibility显示文摘Hydroxyapatite(HA)bioceramic scaffolds were fabricated by using digital light processing(DLP)based additive manufacturing.Key issues on the HA bioceramic scaffolds,including dispersion,DLP fabrication,sintering,mechanical properties,and biocompatibility were discussed in detail.Firstly,the ffects of dispersant dosage,solid loading,and sintering temperature were studied.The optimal dispersant dosage,solid loading,and sintering temperature were 2wt%,50vol%,and 1250℃,respectively.Then,the mechanical properties and biocompatibility of the HA bioceramic scaffolds were investigated.The DLP-prepared porous HA bioceramic scaffold was found to exhibit excellent mechanical properties and degradation behavior.From this study,DLP technique shows good potential for manufacturing HA bioceramic scaffolds. | Chengwei FENG Keqiang ZHANG Rujie HE Guojiao DING Min XIA Xinxin JIN Chen XIE | 2020 | Journal of Advanced Ceramics2020，9，3： | 12 |
| 2 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 3 | A Comparative Study on Hydrodynamic Performance of Double Deflector Rectangular Cambered Otter Board显示文摘In the present work,the hydrodynamic performance of the double deflector rectangular cambered otter board was studied using wind tunnel experiment,flume tank experiment and numerical simulation.Results showed that the otter board had a good hydrodynamic performance with the maximum lift-to-drag ratio(K_(MAX) = 3.70).The flow separation occurred when the angle of attack(AOA) was at 45?,which revealed that the double deflector structure of the otter board can delay the flow separation.Numerical simulation results showed a good agreement with experiment ones,and could predict the critical AOA,which showed that it can be used to study the hydrodynamic performance of the otter board with the advantage of flow visualization.However,the drag coefficient in flume tank was much higher than that in wind tunnel,which resulted in a lower lift-to-drag ratio.These may be due to different fluid media between flume tank and wind tunnel,which result in the big difference of the vortexes around the otter board.Given the otter boards are operated in water,it was suggested to apply both flume tank experiment and numerical simulation to study the hydrodynamic performance of otter board. | XU Qingchang FENG Chunlei HUANG Liuyi XU Jiqiang WANG Lei ZHANG Xun LIANG Zhenlin TANG Yanli ZHAO Fenfang WANG Xinxin | 2018 | Journal of Ocean University of China2018，17，5： | 5 |
| 4 | Zonal method solution of radiative heat transfer in a one-dimensional long roller-hearth furnace in CSP显示文摘A radiative heat transfer mathematical model for a one-dimensional long furnace was set up in a through-type roller-hearth furnace (TTRHF) in compact strip production (CSP). To accurately predict the heat exchange in the furnace, modeling of the complex gas energy-balance equation in volume zones was considered, and the heat transfer model of heating slabs and wall lines was coupled with the radiative heat transfer model to identify the surface zonal temperature. With numerical simulation, the temperature fields of gas, slabs, and wall lines in the furnace under one typical working condition were carefully accounted and analyzed. The fundamental theory for analyzing the thermal process in TTRHF was provided. | Wenfei Wu Yanhui Feng Xinxin Zhang | 2007 | Journal of University of Science and Technology Beijing2007，14，4： | 4 |
| 5 | Optimization of a serpentine flow field with variable channel heights and widths for PEM fuel cells显示文摘The present study proposes a modified serpentine flow field design in which the channel heights vary along each straight flow path to enhance reactant transport and liquid water removal. An optimization approach, combining a simplified conjugate-gradient method (inverse solver) and a three-dimensional, two-phase, non-isothermal fuel cell model (direct solver), has been developed to optimize the key geometric parameters. The optimal design has tapered channels for channels 1, 3 and 4 and increasing heights for channels 2 and 5 with the flow widths first increasing and then decreasing. The optimal channel heights and widths enhance the efficiency by 22.51% compared with the basic design having all heights and widths of 1 mm. The diverging channels have a greater impact on cell performance than fine adjustments of the channel widths for the present simulation conditions. The channel heights have more effect on the sub-rib convection, while the channel widths affect the uniformity of the fuel delivery more. The reduced channel heights of channels 2–4 significantly enhance the sub-rib convection to effectively transport oxygen to and liquid water out of the diffusion layer. The final diverging channel prevents significant leakage of fuel to the outlet via sub-rib convection. | LIN Lin, ZHANG XinXin, FENG HuTing & WANG XiaoDong Department of Thermal Engineering, School of Mechanical Engineering, University of Science and Technology Beijing, Bejing 100083, China | 2010 | Science China(Technological Sciences)2010，53，2： | 4 |
| 6 | Influence of the NAO on Wintertime Surface Air Temperature over East Asia:Multidecadal Variability and Decadal Prediction显示文摘In this paper,we investigate the influence of the winter NAO on the multidecadal variability of winter East Asian surface air temperature(EASAT)and EASAT decadal prediction.The observational analysis shows that the winter EASAT and East Asian minimum SAT(EAmSAT)display strong in-phase fluctuations and a significant 60-80-year multidecadal variability,apart from a long-term warming trend.The winter EASAT experienced a decreasing trend in the last two decades,which is consistent with the occurrence of extremely cold events in East Asia winters in recent years.The winter NAO leads the detrended winter EASAT by 12-18 years with the greatest significant positive correlation at the lead time of 15 years.Further analysis shows that ENSO may affect winter EASAT interannual variability,but does not affect the robust lead relationship between the winter NAO and EASAT.We present the coupled oceanic-atmospheric bridge(COAB)mechanism of the NAO influences on winter EASAT multidecadal variability through its accumulated delayed effect of~15 years on the Atlantic Multidecadal Oscillation(AMO)and Africa-Asia multidecadal teleconnection(AAMT)pattern.An NAO-based linear model for predicting winter decadal EASAT is constructed on the principle of the COAB mechanism,with good hindcast performance.The winter EASAT for 2020-34 is predicted to keep on fluctuating downward until~2025,implying a high probability of occurrence of extremely cold events in coming winters in East Asia,followed by a sudden turn towards sharp warming.The predicted 2020/21 winter EASAT is almost the same as the 2019/20 winter. | Jianping LI Tiejun XIE Xinxin TANG Hao WANG Cheng SUN Juan FENG Fei ZHENG Ruiqiang DING | 2022 | Advances in Atmospheric Sciences2022，39，4： | 4 |
| 7 | Early assessment of the safety and immunogenicity of a third dose(booster)of COVID-19 immunization in Chinese adults显示文摘Inducing durable and effective immunity against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)via vaccination is essential to combat the current pandemic of coronavirus disease 2019(COVID-19).It has been noticed that the strength of anti-COVID-19 vaccination-induced immunity fades over time,which calls for an additional vaccination regime,as known as booster immunization,to restore immunity among previously vaccinated populations.Here we report a pilot open-label trial of a third dose of BBIBP-CorV,an inactivated SARS-CoV-2 vaccine(Vero cell),on 136 participants aged between 18 to 63 years.Safety and immunogenicity in terms of neutralizing antibody titers and cytokine/chemokine responses were analyzed as the main endpoint until day 28.While systemic reactogenicity was either absent or mild,SARS-CoV-2-specific neutralizing antibody titers rapidly arose in all participants within 4 weeks,surpassing the peak antibody titers elicited by the initial two-dose immunization regime.Broad increases of cellular immunity-associated cytokines and chemokines were also detected in the majority of participants after the third vaccination.Furthermore,in an exploratory study,a newly developed recombinant protein vaccine,NVSI-06-08(CHO Cells),was found to be safe and even more effective than BBIBP-CorV in eliciting humoral immune responses in BBIBP-CorV-primed individuals.Together,these results indicate that a third immunization schedule with either homologous or heterologous vaccine showed favorable safety profiles and restored potent SARS-CoV-2-specific immunity,providing support for further trials of booster vaccination in larger populations. | Yuntao Zhang Yunkai Yang Niu Qiao Xuewei Wang Ling Ding Xiujuan Zhu Yu Liang Zibo Han Feng Liu Xinxin Zhang Xiaoming Yang | 2022 | Frontiers of Medicine2022，16，1： | 3 |
| 8 | Late Tremadoc and Early Floian Graptolites from Central Hunan,China显示文摘迟了的 Tremadoc 和早 Floian 的十四 graptolite 种在 Yiyang 区域从 Nanba 节被描述,湖南省。在他们之中, Paradelograptus onubensis, Kiaerograptus bulmani, K。迟了的 Tremadoc 的 kutchini 和 Paratemnograptus isolatus 首先在中国被发现。基于当前的 graptolite 材料,在中国的上面的 Tremadocian 的相应地完全的 graptolite 地区如下被认出(在登上顺序) :Adelograptus tenellus 地区, Aorograptus victoriae 地区, Araneograptus murrayi 地区和 Hunnegraptus copiosus 地区。在 Nanba 节, Hunnegraptus copiosus 地区被 Tetragraptus 直接覆盖接近地区,在 Floian 的底下面没有中断。 | LIMing FENG Hongzhen JI Xinxin LILixia | 2010 | Acta Geologica Sinica(English Edition)2010，84，6： | 3 |
| 9 | Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity显示文摘The continuing challenges that limit effectiveness of tumor therapeutic vaccines were high heterogeneity of tumor immunogenicity, low bioactivity of antigens, as well as insufficient lymph nodes(LNs) drainage of antigens and adjuvants. Transportation of in situ neoantigens and adjuvants to LNs may be an effective approach to solve the abovementioned problems. Therefore, an FA-TSL/AuNCs/SV nanoplatform was constructed by integrating simvastatin(SV) adjuvant loaded Au nanocages(AuNCs)as cores(AuNCs/SV) and folic acid modified thermal-sensitive liposomes(FA-TSL) as shells to enhance de novo antitumor immunity. After accumulation in tumor guided by FA, AuNCs mediated photothermal therapy(PTT) induced the release of tumor-derived protein antigens(TDPAs) and the shedding of FATSL. Exposed AuNCs/SV soon captured TDPAs to form in situ recombinant vaccine(AuNCs/SV/TDPAs). Subsequently, AuNCs/SV/TDPAs could efficiently transport to draining LNs owing to the hyperthermia induced vasodilation effect and small particle size, achieving co-delivery of antigens and adjuvant for initiation of specific T cell response. In melanoma bearing mice, FA-TSL/AuNCs/SV and laser irradiation effectively ablated primary tumor, against metastatic tumors and induced immunological memory. This approach served a hyperthermia enhanced platform drainage to enable robust personalized cancer vaccination. | Cuixia Zheng Xinxin Liu Yueyue Kong Lei Zhang Qingling Song Hongjuan Zhao Lu Han Jiannan Jiao Qianhua Feng Lei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 2 |
| 10 | Computational and Experimental Study of Cooling Process in Coke Dry Quenching Experimental Shaft显示文摘Owing to its merits in energy savings, environmental protection and coke quality improvement, the Coke Dry Quenching (CDQ) technology is favorite in coke making industry today. In order to validate the mathematical model developed for the cooling process of real CDQ shaft, the one-seventh scale experimental setup was established. With the air as the working fluid and the coke as the packed bed, the coke and gas temperatures and the pressure drop in the cooling shaft were measured. A mathematical model based on the non-Darcian and non-thermal equilibrium model for the cooling process of the CDQ experimental shaft was presented. The computational results were compared to the experimental data. It was found that most numerical predictions were supported by the measured values. | Huafei Liu Xinxin Zhang Maolin Wu Yanhui Feng Yingbo Wang Dinan Wang Lie Xu Wenghua Zheng | 2002 | Journal of Thermal Science2002，11，2： | 2 |
| 11 | Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis显示文摘 | Wei Li Baoyang Hu XuepengWang Tianda Li Tongtong Cui Dawei Yu Chao Liu Liyuan Jiang Guihai Feng Lei wang Rui Fu Xinxin Zhang Jie Hao Yukai Wang Liu Wang Qi Zhou | 2018 | Cell Research2018，28，1： | 2 |
| 12 | Comprehensive Characteristics of MicroRNA Expression Profile Conferring to Rhizoctonia solani in Rice显示文摘MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice.To investigate whether miRNAs regulate the resistance to R.solani,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,respectively.By taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the libraries.Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R.solani challenge.Thirty-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction.Moreover,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to R.solani infection confirmed by Northern blot.Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R.solani.Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease. | CAO Wenlei CAO Xinxin ZHAO Jianhua ZHANG Zhaoyang FENG Zhiming OUYANG Shouqiang ZUO Shimin | 2020 | Rice science2020，27，2： | 2 |
| 13 | Numerical and experimental study on coke size distribution in bell-type charging in the CDQ shaft显示文摘The mechanism of coke size segregation in the radial direction of pre-chamber in coke dry quenching(CDQ)shaft was investigated by experiment and numerical analysis.The experimental apparatus was a cold three-dimensional semicircle model for bell-type charging,which was used to study the influences of different kinds of bells and different stock line levels on the radial dis- tribution of coke size in the radial direction of a pre-chamber.A mathematical model was used,which is capable of estimating the radial average size distribution as well as the radial deposit distribution of each particle size for multiple size coke with a few fixed parameters.The calculated results agree well with the experimental data,which proves the reliability of both experimental study and numerical analysis. | Zhicheng Liu Yanhui Feng Xinxin Zhang Lie Xu Zhendong Yu | 2008 | Journal of University of Science and Technology Beijing2008，15，3： | 2 |
| 14 | Assessing the genetic diversity of the critically endangered Chinese sturgeon Acipenser sinensis using mitochondrial markers and genome-wide single-nucleotide polymorphisms from RAD-seq显示文摘As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2006, for economic purposes.The natural population of Chinese sturgeon has declined since then, as these dams block its migratory route to the original spawning grounds in the middle reaches of the Yangtze River. In 2013 and 2014, there was an absence of spawning where it typically happened near the Gezhouba Dam. Nevertheless, from April to June in 2015, over 1,000 larvae with different body lengths(10–35 cm) were detected along the Shanghai Yangtze Estuary; but only little is currently known about the population genetic structure of the Chinese sturgeon. Herein, we inferred population genetic parameters from 462 available Chinese sturgeon specimens based on a 421-bp fragment of the mitochondrial DNA(mtDNA) D-loop region and 1,481,620 SNPs(singlenucleotide polymorphisms) generated by restriction site-associated DNA sequencing(RAD-seq). For the D-loop dataset, 15 haplotypes were determined. Randomly picked 23 individuals, representing the 15 D-loop haplotype groups, were subsequently used for further RAD-seq validation. The average nucleotide diversity calculated from the mtDNA and RAD datasets was 0.0086 and 0.000478, respectively. The overall effective female population size was calculated to be 1,255 to 2,607, and the long-term effective population size was estimated to range from 11,950 to 119,500. We observed that the genetic variability and the effective female population size of the current population in the Yangtze River are severely low, which are similar to the data reported over 10 years ago. The deduced relatively small effective population of female fish, limiting the genetic connectivity among Chinese sturgeon, should be considered a serious threat to this endangered species. | Jian Liu Xinxin You Pao Xu Ping Zhuang Yueping Zheng Kai Zhang Min Wang Yunyun Lv Gangchun Xu Feng Zhao Jianhui Wu Houyong Fan Jianan Xu Zhiqiang Ruan Chao Bian Kai Liu Dongpo Xu Jinhui Chen Junmin Xu Qiong Shi | 2018 | Science China(Life Sciences)2018，61，9： | 2 |
| 15 | IRE1α is essential for Xenopus pancreas development显示文摘Inositol requiring enzyme-1(IRE1) is highly conserved from yeasts to humans.Upon the endoplasmic reticulum(ER) stress,IRE1 activates X-box-binding protein 1(XBP1) by unconventionally splicing XBP1 mRNA,which activates the unfolded protein response(UPR) to restore ER homeostasis.In mice,IRE1α inactivity leads to embryonic death and IRE1α plays an essential role in extraembryonic tissues and the placenta.However,its precise action in the embryo proper is still unknown.In this study,the loss of function analysis was performed to investigate the function of Xenopus IRE1α(xIRE1α) during pancreas development.Firstly,the complete open reading frame of xIRE1α was amplified and the expression pattern was detected.The effects of Xenopus IRE1αand XBP1 during embryo development were detected with whole-mount in situ hybridization.The results demonstrated that xIRE1α was much closer to human IRE1α when compared with their sequence alignment.xIRE1αwas expressed strongly in developing pancreas and the knockdown of xIRE1α inhibited the differentiation and specification of the pancreas.xIRE1α,which was required for cytoplasmic splicing of XBP1 pre-mRNA and XBP1 MO,also showed inhibitory effects on pancreas development.These results suggest that xIRE1α is essential for pancreas development during embryogenesis and functions via the XBP1 dependent pathway. | Li Yuan Xinxin Li Jiaojiao Feng Chenyang Yin Fang Yuan Xinru Wang | 2014 | The Journal of Biomedical Research2014，28，2： | 2 |
| 16 | The leaf extract of crofton weed(Eupatorium adenophorum)inhibits primary root growth by inducing cell death in maize root border cells显示文摘The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear.In this study,we investigated the effects of the leaf extract of crofton weed(LECW) on primary root(PR) growth in maize seedlings.Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner.Physiological analysis indicated that the LECW induced reactive oxygen species(ROS) accumulation in root tips,thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips,finally leading to cell death in root border cells(RBCs) and PR growth inhibition.The LECW also inhibited pectin methyl esterase(PME) activity,thereby decreasing the RBC number.Taken together,our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in RBCs.The present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants. | Jinhu Ma Xinxin Feng Xiaohuan Yang Yongheng Cao Weifeng Zhao Liangliang Sun | 2020 | Plant Diversity2020，42，3： | 2 |
| 17 | Multiplexed cytokine profiling of serum for detection of colorectal cancer显示文摘 | Zhang Pengjun Wen Xinyu Gu Feng Deng Xinxin Liu Yulan Li Juan Jia Xingwang Dong Zhennan Tian Yaping | 2013 | Future Oncol2013，，7： | 2 |
| 18 | Evaluation of the ventilation system in an LNG cargo tank construction platform (CTCP) by the AHP-entropy weight method显示文摘Owing to the vulnerability of Invar to moisture in the membranes of LNG tanker cargo tank construction platforms(CTCPs),an energy-efficient ventilation system is needed to maintain a suitable thermal-moisture environment and a healthy workplace.However,the optimal distribution of supply and return devices that would guarantee worker satisfaction,air quality,and energy efficiency is unclear.Therefore,we conducted numerical simulations to determine the worker satisfaction indices(temperature,relative humidity,and carbon monoxide concentration satisfactions),air quality index(contaminant-removal efficiency),and energy efficiency index(heat-removal efficiency)with supply vane angles ranging from-75°to 0°and two return vent positions(the bottom return vent and the top return vent).The analytic hierarchy process(AHP)entropy weight method was employed to determine the optimal supply vane angle and return vent position for three design targets by considering these indices simultaneously.The results indicated that,with a supply angle of-45°and a bottom return vent,worker satisfaction and air quality were prioritized.Furthermore,a high energy performance of the ventilation system was achieved with a-15°supply angle and a bottom return vent.Moreover,a comprehensive graph of supply vane angles at both return heights,which could provide a reference for optimizing the ventilation system in LNG-CTCPs,is described. | Dachuan Shi Yuejiao Guo Xinxin Gu Guozeng Feng Yang Xu Shaozhe Sun | 2022 | Building Simulation2022，15，7： | 2 |
| 19 | Effects of sulfide on microbial fuel cells with platinum and nitrogen-doped carbon powder cathodes显示文摘 | Yujie Feng Xinxin Shi Xin Wang He Lee Jia Liu Youpeng Qu Weihua He S.M. Senthil Kumar Byung Hong Kim Nanqi Ren | 2011 | Biosensors and Bioelectronics2011，，1： | 1 |
| 20 | Synthesis of confined Ag nanowires within mesoporous silica via double solvent technique and their catalytic properties显示文摘 | Xiubing Huang Wenjun Dong Ge Wang Mu Yang Li Tan Yanhui Feng Xinxin Zhang | 2011 | Journal of Colloid And Interface Science2011，，1： | 1 |
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