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| 1 | The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming显示文摘新陈代谢对房间功能的每个方面重要,然而,导致的 pluripotent 干细胞(iPSCs ) 的 metabolome 仍然保持大部分未经勘探。这里,我们报导,用一条 untargeted metabolomics 途径,那人的 iPSCs 份额有从他们的父母房间是不同的胚胎的干细胞(转换字符) 的 pluripotent metabolomic 签名,并且那被变化在涉及细胞的呼吸的代谢物描绘。细胞的 bioenergetics 的检查与我们的 metabolomic 分析支持了,并且证明体的房间在 pluripotency 从一个氧化状态变换成一个 glycolytic 状态。有趣地,各种各样的体的房间的 bioenergetics 与他们的 reprogramming 效率相关。我们进一步识别了在 iPSCs 和转换字符之间不同的代谢物,它揭示了在调整体的房间 reprogramming 起一个关键作用的新奇新陈代谢的小径。我们的调查结果是第一全球性分析 iPSCs 的 metabolome,并且提供机械学的卓见进涉及导致 pluripotency,并且在评估 iPSC 和转换字符等价的规定的新层。 | Athanasia D Panopoulos Oscar Yanes Sergio Ruiz Yasuyuki S Kida Dinh Diep Ralf Tautenhahn Aida Herrerias Erika M Batchelder Nongluk Plongthongkum Margaret Lutz W Travis Berggren Kun Zhang Ronald M Evans Gary Siuzdak Juan Carlos Izpisua Belmonte] | 2012 | Cell Research2012，22，1： | 12 |
| 2 | 植物叶缘锯齿发育的研究进展显示文摘叶缘锯齿是叶片重要的形态特征之一,受到多种叶缘发育因子的调控。笔者回顾了国内外研究成果,详细分析了植物激素、CUC2(CUP SHAPED COTYLEDONE2)和MicroRNA对叶缘锯齿发育的影响,总结了植物中各叶缘因子的表达与功能,并探讨了叶缘锯齿形成的分子机制及其意义。分析表明,在复杂的叶缘发育调控网络中,生长素与CUC2对叶缘的调控起到非常重要的作用,大部分叶缘因子的调控功能依赖于生长素和CUC2。最后,展望了叶缘锯齿发育未来的研究方向与发展趋势,并对其以后的应用领域进行了预测。 | 李晓屿 Ralf Müller-Xing Shahid Khan 郑玉彩 李玉花 邢倩 | 2019 | 中国农学通报2019，35，1： | 6 |
| 3 | 塔里木盆地煤及其显微组分超高温开放体系热模拟实验气态产物对比研究显示文摘通过对塔里木盆地煤及其显微组分进行以1K/min升温速率的开放体系热模拟实验(最高实验温度为1200℃),获得了煤岩与各显微组分气态产物产率与累计产量变化。对比分析了煤及其显微组分生成气态产物CH4、N2和CO变化特征,其中N2生成温度高于CH4和CO;H2生成时间与CH4相一致(说明了氢为烃类气体形成的关键因素)。煤及其显微组分生烃潜力具有壳质组>镜质组>煤>半丝质组≥丝质组的特征,说明煤岩生烃潜力主要由其显微组分控制;虽然壳质组在煤岩中含量很低,但生烃潜力高于其他显微组分。煤岩生成的N2具有双峰型特征,说明了前后峰N2具有不同来源,前者来源于无机矿物(如含铵粘土)的分解,后者来源于有机质热降解;显微组分生成N2主要为高温阶段,说明了显微组分在分离过程中可能造成部分无机矿物的损失。 | 刘全有 Bernhard M Krooss 金之钧 王毅 Jan Hollenstein Ralf Littke 刘文汇 | 2008 | 天然气地球科学2008，19，6： | 5 |
| 4 | Mobilization of hematopoietic progenitor cells in patients with liver cirrhosis显示文摘AIM:To test the hypothesis that liver cirrhosis is associated with mobilization of hematopoietic progenitor cells. METHODS:Peripheral blood samples from 72 patients with liver cirrhosis of varying etiology were analyzed by flow cytometry.Identified progenitor cell subsets were immunoselected and used for functional assays in vitro. Plasma levels of stromal cell-derived factor-1(SDF-1) were measured using an enzyme linked immunosorbent assay.RESULTS:Progenitor cells with a CD133 + /CD45 + CD14 + phenotype were observed in 61%of th patients.Between 1%and 26%of the peripheral bloo mononuclear cells(MNCs)displayed this phenotype Furthermore,a distinct population of c-kit + progenito cells(between 1%and 38%of the MNCs)could b detected in 91%of the patients.Additionally,18% of the patients showed a population of progenito cells(between 1%and 68%of the MNCs)that wa characterized by expression of breast cancer resistanc protein-1.Further phenotypic analysis disclosed tha the circulating precursors expressed CXC chemokin receptor 4,the receptor for SDF-1.In line with thi finding,elevated plasma levels of SDF-1 were presen in all patients and were found to correlate with th number of mobilized CD133 + progenitor cells. | Ursula M Gehling Marc Willems Kathleen Schlagner Ralf A Benndorf Maura Dandri Jrg Petersen Martina Sterneck Joerg-Matthias Pollok Dieter K Hossfeld Xavier Rogiers | 2010 | World Journal of Gastroenterology2010，16，2： | 4 |
| 5 | 转录因子WRKY6和PR1在拟南芥胁迫记忆中的表达模式显示文摘植物暴露在细菌或其它微生物病原体下,会形成全身防御,称为系统获得性抗性SAR(Systemic Acquired Resistance),该系统可以在病原体二次侵染时有效抑制病原体对植物的伤害。其中,WRKY转录因子和病程相关蛋白PRs(Pathogenesis-related proteins)在植物抗病信号调控途径中起着重要作用。本研究以模式植物拟南芥为实验材料,对WRKY6和PR1(PATHOGENESIS RELATED)两个转录因子进行初步研究。首先,从拟南芥eFP数据库中获得 WRKY6和PR1 的基因表达数据,进行生物信息学分析,获得 WRKY6和PR1 基因在不同胁迫条件下的表达热图。其次,通过实时荧光定量PCR技术,比较了经过生物胁迫和非生物胁迫处理后 WRKY6和PR1 的基因表达水平。结果表明,拟南芥经过生物胁迫丁香假单胞菌[ Pseudomonas syringae pv. tomato ( P st ) DC3000]处理后,WRKY6和PR1的基因表达模式具有一定的相似性,然而经过非生物胁迫和机械损伤组合处理后, WRKY6和PR1基因又呈现出不同的表达模式。本研究初步探索了WRKY6和PR1 基因的表达模式及其关系,为今后进一步研究系统性获得抗性应答机制提供了思路。 | 李爽 熊樱 RALF Müller-Xing 邢倩 | 2019 | 植物研究2019，39，5： | 4 |
| 6 | Role of interleukin-1 and its antagonism of hepatic stellate cell proliferation and liver fibrosis in the Abcb4^(-/-) mouse model显示文摘AIM: To study the interleukin-1(IL-1) pathway as a therapeutic target for liver fibrosis in vitro and in vivo using the ATP-binding cassette transporter b4^(-/-)(Abcb4^(-/-)) mouse model.METHODS: Female and male Abcb4^(-/-) mice from 6 to 13 mo of age were analysed for the degree of cholestasis(liver serum tests), extent of liver fibrosis(hydroxyproline content and Sirius red staining) and tissue-specific activation of signalling pathways such as the IL-1 pathway [quantitative polymerase chain reaction(q PCR)]. For in vivo experiments, murine hepatic stellate cells(HSCs) were isolated via pronasecollagenase perfusion followed by density gradient centrifugation using female mice. Murine HSCs were stimulated with up to 1 ng/m L IL-1β with or without 2.5 μg/m L Anakinra, an IL-1 receptor antagonist, respectively. The proliferation of murine HSCs was assessed via the Brd U assay. The toxicity of Anakinra was evaluated via the fluorescein diacetate hydrolysis(FDH) assay. In vivo 8-wk-old Abcb4^(-/-) mice with an already fully established hepatic phenotype were treated with Anakinra(1 mg/kg body-weight daily intraperitoneally) or vehicle and liver injury and liver fibrosis were evaluated via serum tests, q PCR, hydroxyproline content and Sirius red staining. RESULTS: Liver fibrosis was less pronounced in males than in female Abcb4^(-/-) animals as defined by a lower hydroxyproline content(274 ± 64 μg/g vs 436 ± 80 μg/g liver, respectively; n = 13-15; P < 0.001; MannWhitney U-test) and lower m RNA expression of the profibrogenic tissue inhibitor of metalloproteinase-1(TIMP)(1 ± 0.41 vs 0.66 ± 0.33 fold, respectively; n = 13-15; P < 0.05; Mann-Whitney U-test). Reduced liver fibrosis was associated with significantly lower levels of F4/80 m RNA expression(1 ± 0.28 vs 0.71 ± 0.41 fold, respectively; n = 12-15; P < 0.05; Mann-Whitney U-test) and significantly lower IL-1β m RNA expression levels(1 ± 0.38 vs 0.44 ± 0.26 fold, respectively; n = 13-15; P < 0.001; Mann-Whitney U-test). No gender differences in the serum liver parameters [bilirubin; alanine aminotransferase(ALT); aspartate aminotransferase and alkaline phosphatase(AP)] were found. In vitro, the administration of IL-1β resulted in a significant increase in HSC proliferation [0.94 ± 0.72 arbitrary units(A.U.) in untreated controls, 1.12 ± 0.80 A.U. at an IL-1β concentration of 0.1 ng/m L and 1.18 ± 0.73 A.U. at an IL-1β concentration of 1 ng/m L in samples from n = 6 donor animals; P < 0.001; analyses of variance(ANOVA)]. Proliferation was reduced significantly by the addition of 2.5 μg/m L Anakinra(0.81 ± 0.60 A.U. in untreated controls, 0.92 ± 0.68 A.U. at an IL-1β concentration of 0.1 ng/m L, and 0.91 ± 0.69 A.U. at an IL-1β concentration of 1 ng/m L; in samples from n = 6 donor animals; P < 0.001; ANOVA) suggesting an anti-proliferative effect of this clinically approved IL-1 receptor antagonist. The FDH assay showed this dose to be non-toxic in HSCs. In vivo, Anakinra had no effect on the hepatic hydroxyprolinecontent, liver serum tests(ALT and AP) and profibrotic(collagen 1α1, collagen 1α2, transforming growth factor-β, and TIMP-1) and anti-fibrotic [matrix metalloproteinase 2(MMP2), MMP9 and MMP13 ] gene expression after 4 wk of treatment. Furthermore, the hepatic IL-1β and F4/80 m RNA expression levels were unaffected by Anakinra treatment.CONCLUSION: IL-1β expression is associated with the degree of liver fibrosis in Abcb4^(-/-) mice and promotes HSC proliferation. IL-1 antagonism shows antifibrotic effects in vitro but not in Abcb4^(-/-) mice. | Florian P Reiter Ralf Wimmer Lena Wottke Renate Artmann Jutta M Nagel Manuel O Carranza Doris Mayr Christian Rust Peter Fickert Michael Trauner Alexander L Gerbes Simon Hohenester Gerald U Denk | 2016 | World Journal of Hepatology2016，8，8： | 3 |
| 7 | International Consensus Statement on surveillance and management of dysplasia in inflammatory bowel disease显示文摘 | Loren Laine Tonya Kaltenbach Alan Barkun Kenneth R. McQuaid Venkataraman Subramanian Roy Soetikno James E. East Francis A. Farraye Brian Feagan John Ioannidis Ralf Kiesslich Michael Krier Takayuki Matsumoto Robert P. McCabe Klaus M?nkemüller Robert Odze M | 2014 | Gastrointestinal Endoscopy2014，，： | 3 |
| 8 | Association of the GNAS1 T393C polymorphism with tumor stage and survival in gastric cancer显示文摘AIM:To analyze the impact of the GNAS1 T393C polymorphism on prognosis and histopathology of gastric cancer.METHODS:Genomic DNA was extracted from paraffinembedded tissues of 122 patients with primary gastric carcinoma and from the blood of 820 healthy white individuals.Allelic discrimination was performed by quantitative real-time polymerase chain reaction.Genotyping was correlated with histopathologic parameters and with overall survival according to the Kaplan-Meier approach and with multivariate analysis by multiple stepwise regression.RESULTS:Thirty-nine(32%) patients displayed a CC genotype,57(46.7%) a CT genotype and 26(21.3%) a TT genotype.The frequency of the C allele(fC) in the patient group was 0.55,which was not signif icantly different from that of healthy blood donors.The distribution was compatible with the Hardy-Weinberg equilibrium.Analysis of clinicopathological parameters did not show any signif icant correlation of the T393C genotype with gender(P=0.50),differentiation(P=0.29),pT-category(P=0.19),pN-category(P=0.30),pM-category(P=0.25),R-category(P=0.95),the classifications according to WHO(P=0.34),Laurén(P=0.16),Goseki(P=1.00) and Ming(P=0.74).Dichotomization between C+(CC+CT) and C-genotypes(TT),however,revealed signif icantly more advanced tumor stages(P=0.023) and lower survival rates(P=0.043) for C allele carriers.CONCLUSION:The present study provides strong evidence to suggest that the GNAS1 T393C allele carrier status influences tumor progression and survival in gastric cancer with higher tumor stages and a worse outcome for C allele carriers. | Hakan Alakus Stefan P Mnig Ute Warnecke-Eberz Gül Alakus Günther Winde Uta Drebber Klaus J Schmitz Kurt W Schmid Kathrin Riemann Winfried Siffert Elfriede Bollschweiler Arnulf H Hlscher Ralf Metzger | 2009 | World Journal of Gastroenterology2009，15，48： | 3 |
| 9 | A prospective two-center study comparing wireless capsule endoscopy with intraoperative enteroscopy in patients with obscure GI bleeding显示文摘 | Dirk Hartmann Harald Schmidt Georg Bolz Dieter Schilling Frank Kinzel Axel Eickhoff Winfried Huschner Kathleen M?ller Ralf Jakobs Peter Reitzig Uwe Weickert Klaus Gellert Harald Schultz Klaus Guenther Hartmut Hollerbuhl Klaus Schoenleben Hans-Joachim Schu | 2005 | Gastrointestinal Endoscopy2005，，7： | 2 |
| 10 | Mobilization and functionality of endothelial progenitor cells during antihypertensive treatment in patients with essential hypertension显示文摘 | Ralf A B Ulla M G Daniel A | 2004 | Circulation2004，110，17： | 1 |
| 11 | Polybrominated diphenyl ethers (PBDEs) and alternative brominated flame retardants in air and seawater of the European Arctic显示文摘 | Axel M?ller Zhiyong Xie Renate Sturm Ralf Ebinghaus | 2011 | Environmental Pollution2011，，6： | 1 |
| 12 | FGF5 and the murine hair cycle显示文摘 | Attila Peth?-Schramm Hans-Joachim Müller Ralf Paus | 1996 | Archives of Dermatological Research (-)1996，，5： | 1 |
| 13 | Mechanochemical Route to Functionalized Graphene and Carbon Nanofillers for Graphene/SBR Nanocomposites显示文摘 | Fabian Beckert Stephanie Trenkle Ralf Thomann Rolf Mülhaupt | 2014 | Macromol. Mater. Eng2014，，12： | 1 |
| 14 | Mutations of Helicobacterpylori genes rdxA and pbp1 cause resistance against metronidazole显示文摘 | Ralf P Stefan P Klaus M | 2001 | Antimicrob Agents Chemother2001，45，3： | 1 |
| 15 | Effects of fungicide and insecticide mix-tures on apple tree canopy photosynthesis,dark respiration andcarbon economy显示文摘 | Ralf U Michael M B | 2004 | Crop Protection2004，23，： | 1 |
| 16 | Sorafenib in radioactive iodine-refractory, locally advanced or metastatic differentiated thyroid cancer: a randomised, double-blind, phase 3 trial显示文摘 | Marcia S Brose Christopher M Nutting Barbara Jarzab Rossella Elisei Salvatore Siena Lars Bastholt Christelle de la Fouchardiere Furio Pacini Ralf Paschke Young Kee Shong Steven I Sherman Johannes W A Smit John Chung Christian Kappeler Carol Pe?a István Mo | 2014 | The Lancet2014，，： | 1 |
| 17 | A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to Ke Feng 6显示文摘 | RALF R LUTZ G DIETRICH M | 2010 | Journal für Verbraucherschutz und Lebensmittelsicherheit2010，5，2： | 1 |
| 18 | Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array 显示文摘 | Wang SC Debbie W Kerrie F Alexandra A Shiaoman C Bevan EH Marco M Silvio S Sara GM Luigi C Anna MM Alex W Stuart S Gary B Ralf W Joerg P International Wheat Genome Sequencing Consortium Morten L Diane M Rudi A Rudy D Gina BG Abraham K Alina RA Catherine F Jerome S Michele M Curtis P Luo MC Jan DMM Jorge D Martin G Roberto T Cindy L Ivan M Colin C Keith JE Matthew H Eduard A | 2014 | Plant Biotechnol J2014，12，： | 1 |
| 19 | Sixteen-slice spiral CT versus MR imaging for the assessment of left ventricular function in acute myocardial infarcion显示文摘 | Andreas H M Ralf K Marcus K | 2005 | Eur Radiol2005，15，1： | 1 |
| 20 | A mobile robot employing insect strategies for navigation显示文摘 | Dimitrios Lambrinos Ralf M?ller Thomas Labhart Rolf Pfeifer Rüdiger Wehner | 2000 | Robotics and Autonomous Systems2000，，1： | 1 |
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