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| 1 | Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis显示文摘AIM:To investigate matrix metalloproteinases(MMPs) and their tissue inhibitors(TIMPs) in pouch mucosa of pediatric onset ulcerative colitis(UC).METHODS:In this cross-sectional study,28 patients with pediatric onset UC underwent ileal pouch biopsy 13 years(median) after proctocolectomy.Expression of MMPs-3,-7,-8,-9,-12 and-26 and TIMPs-1,-2 and-3 in samples was examined using immunohistochemichal methods,and another biopsy was used to evaluate the grade of histological inflammation.Two investigators independently graded the immunohistochemical specimens in a semiquantitative fashion,using a scale marking staining intensity as follows:0 = less than 20 positive cells;1 = 20-50 positive cells;2 = 50-200 positive cells;3 = over 20 positive cells.Fecal calprotectin and blood inflammatory markers [serum C-reactive protein(CRP) and erythrocyte sedimentation rate] were determined during a follow-up visit to examine correlations between these markers and the expression of MMPs and TIMPs.RESULTS:Of the 28 patients with pediatric onset UC,nine had not experienced pouchitis,whereas thirteen reported a single episode,and six had recurrent pouchitis(≥ 4 episodes).At the time of the study,six patients required metronidazole.In all of the others,the most recent episode of pouchitis had occurred over one month earlier,and none were on antibiotics.Only four samples depicted no sign of inflammation,and these were all from patients who had not had pouchitis.Two samples were too small to determine the grade of inflammation,but both had suffered pouchitis,the other recurrent.No sample depicted signs of colonic metaplasia.Most pouch samples showed expression of epithelial(e) and stromal(s) MMP-3(e,n = 22;s,n = 20),MMP-7(e,n = 28;s,n = 27),MMP-12(e,n = 20;s,n =24),TIMP-2(e,n = 23;s,n = 23) and MMP-3(e,n = 23;s,n = 28) but MMP-8(e,n = 0;s,n = 1),MMP-9(e,n = 0;s,n = 9) and MMP-26(e,n = 0;s,n = 3) and TIMP-1(n = 0,both) were lacking.In samples with low grade of inflammatory activity,the epithelial MMP-3 and MMP-7 expression was increased(r =-0.614 and r =-0.472,respectively,P < 0.05 in both).MMPs and TIMPs did not correlate with the markers of inflammation,fecal calprotectin,erythrocyte sedimentation rate,or CRP,with the exception of patients with low fecal calprotectin(< 100 μg/g) in whom a higher expression of epithelial MMP-7 was found no differences in MMPor TIMP-profiles were seen in patients with a history of pouchitis compared to ones with no such episodes.Anastomosis with either straight ileoanal anastomosis or ileoanal anastomosis with J-pouch did depict differences in MMP-or TIMP-expression.CONCLUSION:The expression of MMPs pediatric UC pouch in the long-term shares characteristics with inflammatory bowel disease,but inflammation cannot be classified as a reactivation of the disease. | Laura Mkitalo Maija Piekkala Merja Ashorn Mikko Pakarinen Antti Koivusalo Riitta Karikoski Johanna Natunen Ulpu Saarialho-Kere Risto Rintala Kaija-Leena Kolho | 2012 | World Journal of Gastroenterology2012，18，30： | 2 |
| 2 | Evolution of aluminosilicate structure and mullite crystallization from homogeneous nanoparticulate sol–gel precursor with organic additives显示文摘 | Jarkko Leivo Mika Lindén Jessica M. Rosenholm Merja Ritola Cilaine V. Teixeira Erkki Lev?nen Tapio A. M?ntyl? | 2008 | Journal of the European Ceramic Society2008，，9： | 1 |
| 3 | Teaching evidence-based medicine using literature for problem solving显示文摘 | M? tt? nen Merja Tapanainen P? ivi Nuutinen Matti | 2001 | Medical Teacher2001，23，1： | 1 |
| 4 | Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae 显示文摘 | Merja E Penttila M Andre L | 1987 | Yeast1987，3，3： | 1 |
| 5 | Cyclical regulation of the insulin-like growth factor binding protein 3 gene in response to la,25-dihydroxyvitamin D3显示文摘 | Marjo M Jussi R Merja H | | 0，，02： | 1 |
| 6 | Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae显示文摘 | MERJA E PENTTILA M ANDRE L | 1987 | Yeast1987，3，3： | 1 |
| 7 | Regulation of multiple insulin-like growth factor binding protein genes by la,25-dthydroxy-vitamin D3显示文摘 | Merja M Marjo M Katri S | | 0，，17： | 1 |
| 8 | Enhancement of percutaneous absorption of naproxen by phospholipids显示文摘 | Riitta VK Merja K Jukka M | 1998 | Int JPharm1998，175，2： | 1 |
| 9 | Systematic Identification of MicroRNAs That Impact on Proliferation of Prostate Cancer Cells and Display Changed Expression in Tumor Tissue显示文摘 | Anna Aakula Pekka Kohonen Suvi-Katri Leivonen Rami M?kel? Petteri Hintsanen John Patrick Mpindi Elena Martens-Uzunova Tero Aittokallio Guido Jenster Merja Per?l? Olli Kallioniemi P?ivi ?stling | 2015 | European Urology2015，，： | 1 |
| 10 | High endothelial venules of the lymph nodes express Fas/igand显示文摘 | Tuomo S Merja T Johanna M | 2004 | J Histoch & Cytoch2004，52，： | 1 |
| 11 | Synthesis and biological evalution of pyridopyrimidine and isoxazoline derivatives显示文摘 | Merja B C Joshi A M Parikh K A | 2004 | Indian J Chem(Section B)2004，，4： | 1 |
| 12 | Current Strategies for the Treatment Hereditay Tyrosinemia Type I 显示文摘 | Ashorn Merja Pitkanen Sari Calo M K | 2006 | Pedlatr Drugs2006，8，1： | 1 |
| 13 | Effect of anaesthesia on contrast-enhanced ultrasound of the feline spleen显示文摘 | Merja R L Marja R R Outi M V | | 0，，： | 1 |
| 14 | Short-term weight change and fluctuation as risk factors for type 2 diabetes in Finnish male smokers显示文摘 | Merja Kataja-Tuomola Jari Sundell Satu M?nnist? Mikko J. Virtanen Jukka Kontto Demetrius Albanes Jarmo Virtamo | 2010 | European Journal of Epidemiology2010，，5： | 1 |
| 15 | The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves 显示文摘 | Annika M M Merja M Gyllenberg H | 1983 | Journal of Applied Bacteriology1983，55，： | 1 |
| 16 | High-throughput screens identify microRNAs essential for HER2 positive breast cancer cell growth显示文摘 | Suvi-Katri Leivonen Kristine Kleivi Sahlberg Rami M?kel? Eldri Undlien Due Olli Kallioniemi Anne-Lise B?rresen-Dale Merja Per?l? | 2014 | Molecular Oncology2014，，1： | 1 |
| 17 | Cloning of Aspergillus niger genes in yeast, expression of the gene coding Aspergillus β-glucosidase 显示文摘 | Merja E Penttila K M Helena N | 1984 | Mol Gen Genet1984，194，10： | 1 |
| 18 | Longitudinal Follow-up of Bronchial Inflammation, Respiratory Symptoms, and Pulmonary Function in Adolescents after Repair of Esophageal Atresia with Tracheoesophageal Fistula显示文摘 | Kristiina Malmstr?m Jouko Lohi Harry Lindahl Anna Pelkonen Merja Kajosaari Seppo Sarna L. Pekka Malmberg Mika J. M?kel? | 2008 | The Journal of Pediatrics2008，，3： | 1 |
| 19 | Infections, Inflammation, and the Risk of Coronary Heart Disease显示文摘 | Merja Roivainen Maarit Viik-Kajander Timo Palosuo Petri Toivanen Maija Leinonen Pekka Saikku Leena Tenkanen Vesa Manninen Tapani Hovi Matti M?ntt?ri | 2000 | Circulation: Journal of the American Heart Association2000，，3： | 1 |
| 20 | Contents of phytochemicals and antinutritional factors in commercial protein-rich plant products显示文摘Objectives:The goal of this study was to determine several beneficial and antinutritional compounds in the commercial products of some protein-rich crops.Materials and Methods:Samples of faba bean(Vicia faba),lupin(Lupinus angustifolius),rapeseed press cake(Brassica rapa/napus subsp.Oleifera),flaxseed(Linum usitatissimum),oil hemp seed(Cannabis sativa),buckwheat(Fagopyrum esculentum),and quinoa(Chenopodium quinoa)were purchased from the grocery stores and from the producers.The phytochemicals were analyzed using various validated methods.Results:Rape seed press cake contained the highest contents of phenolics.The highest contents of proanthocyanidins were found in faba bean(470±14 mg/100g DW).Secoisolariciresinol-diglucoside content in the flaxseeds was 138±32 mg/100 g DW and the lignanamide content of hemp seeds was 77±22 mg/100 g DW.The content of phytic acid in rapeseed cake,hemp seed and flaxseed samples was 3.9±0.1,3.5±0.2,and 1.9±0 g/100 g DW,respectively.Rape seed cake contained glucosinolates 2.08±0.74 umol/g DW and faba bean contained vicine and convicine 0.77±0.11 and 0.42±0.02 g/100 g DW,respectively.Quinoa contained saponins 557 mg/100 g DW and after abrasive milling/pearling the content was 36 mg/100 g DW.Generally,the phytochemicals were concentrated in the outer shell of the seed.Inhibitory activities against proteolytic enzymes,trypsin andα-chymotrypsin,varied among the samples analyzed.Highest activity was found in buckwheat samples,whereas lupin and rapeseed press cake had no inhibitory activity against tested enzymes.Conclusions:This paper presented new findings on the contents of phytochemicals in various under-used plant protein sources. | Pirjo H.Mattila Juha-Matti Pihlava Jarkko Hellström Markus Nurmi Merja Eurola Sari Mäkinen Taina Jalava Anne Pihlanto | 2018 | Food Quality and Safety2018，2，4： | 0 |
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