|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV) infected pneumonia(standard version)显示文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named '2019 novel coronavirus(2019-nCoV)' by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV. | Ying-Hui Jin Lin Cai Zhen-Shun Cheng Hong Cheng Tong Deng Yi-Pin Fan Cheng Fang Di Huang Lu-Qi Huang Qiao Huang Yong Han Bo Hu Fen Hu Bing-Hui Li Yi-Rong Li Ke Liang Li-Kai Lin Li-Sha Luo Jing Ma Lin-Lu Ma Zhi-Yong Peng Yun-Bao Pan Zhen-Yu Pan Xue-Qun Ren Hui-Min Sun Ying Wang Yun-Yun Wang Hong Weng Chao-Jie Wei Dong-Fang Wu Jian Xia Yong Xiong Hai-Bo Xu Xiao-Mei Yao Yu-Feng Yuan Tai-Sheng Ye Xiao-Chun Zhang Ying-Wen Zhang Yin-Gao Zhang Hua-Min Zhang Yan Zhao Ming-Juan Zhao Hao Zi Xian-Tao Zeng Yong-Yan Wang Xing-Huan Wang 无 | 2020 | Military Medical Research2020，7，1： | 161 |
| 2 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 3 | High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa显示文摘It has been reported that ACE2 is the main host cell receptor of 2019-nCoV and plays a crucial role in the entry of virus into the cell to cause the final infection. To investigate the potential route of 2019-n Cov infection on the mucosa of oral cavity, bulk RNA-seq profiles from two public databases including The Cancer Genome Atlas(TCGA) and Functional Annotation of The Mammalian Genome Cap Analysis of Gene Expression(FANTOM5 CAGE) dataset were collected. RNA-seq profiling data of 13 organ types with para-carcinoma normal tissues from TCGA and 14 organ types with normal tissues from FANTOM5 CAGE were analyzed in order to explore and validate the expression of ACE2 on the mucosa of oral cavity. Further, single-cell transcriptomes from an independent data generated in-house were used to identify and confirm the ACE2-expressing cell composition and proportion in oral cavity. The results demonstrated that the ACE2 expressed on the mucosa of oral cavity. Interestingly, this receptor was highly enriched in epithelial cells of tongue. Preliminarily, those findings have explained the basic mechanism that the oral cavity is a potentially high risk for 2019-nCoV infectious susceptibility and provided a piece of evidence for the future prevention strategy in dental clinical practice as well as daily life. | Hao Xu Liang Zhong Jiaxin Deng Jiakuan Peng Hongxia Dan Xin Zeng Taiwen Li Qianming Chen | 2020 | International Journal of Oral Science2020，12，1： | 69 |
| 4 | Transmission Dynamics of an Outbreak of the COVID-19 Delta Variant B.1.617.2——Guangdong Province,China,May-June 2021显示文摘On May 21,2021,a local case of coronavirus disease 2019(COVID-19)was confirmed in a 75-year-old woman(experienced onset of symptoms on May 18)in Liwan District,Guangzhou City,Guangdong Province,China.The number of infections has increased in the following 10 days and led to 5 generations of transmission. | Meng Zhang Jianpeng Xiao Aiping Deng Yingtao Zhang Yali Zhuang Ting Hu Jiansen Li Hongwei Tu Bosheng Li Yan Zhou Jun Yuan Lei Luo Zimian Liang Youzhi Huang Guoqiang Ye Mingwei Cai Gongli Li Bo Yang Bin Xu Ximing Huang Yazun Cui Dongsheng Ren Yanping Zhang Min Kang Yan Li | 2021 | China CDC weekly2021，3，27： | 51 |
| 5 | Preoperative predictors of portal vein thrombosis after splenectomy with periesophagogastric devascularization显示文摘AIM:To evaluate the predictive value of preoperative predictors for portal vein thrombosis(PVT)after splenectomy with periesophagogastric devascularization.METHODS:In this prospective study,69 continuous patients with portal hypertension caused by hepatitis B cirrhosis underwent splenectomy with periesophagogastric devascularization in West China Hospital of Sichuan University from January 2007 to August 2010.The portal vein flow velocity and the diameter of portal vein were measured by Doppler sonography.The hepatic congestion index and the ratio of velocity and diameter were calculated before operation.The prothrombin time(PT)and platelet(PLT)levels were measured before and after operation.The patients'spleens were weighed postoperatively.RESULTS:The diameter of portal vein was negatively correlated with the portal vein flow velocity(P<0.05).Thirty-three cases(47.83%)suffered from postoperative PVT.There was no statistically significant difference in the Child-Pugh score,the spleen weights,the PT,or PLT levels between patients with PVT and without PVT.Receiver operating characteristic curves showed four variables(portal vein flow velocity,the ratio of velocity and diameter,hepatic congestion index and diameter of portal vein)could be used as preoperative predictors of postoperative portal vein thrombosis.The respective values of the area under the curve were 0.865,0.893,0.884 and 0.742,and the respective cut-off values(24.45 cm/s,19.4333/s,0.1138 cm/s-1 and 13.5 mm) were of diagnostically efficient,generating sensitivity values of 87.9%,93.9%,87.9%and 81.8%,respectively,specificities of 75%,77.8%,86.1%and 63.9%,respectively.CONCLUSION:The ratio of velocity and diameter was the most accurate preoperative predictor of portal vein thrombosis after splenectomy with periesophagogastric devascularization in hepatitis B cirrhosis-related portal hypertension. | Yu Zhang Tian-Fu Wen Lu-Nan Yan Hong-Ji Yang Xiao-Fan Deng Chuan Li Chuan Wang Guan-Lin Liang | 2012 | World Journal of Gastroenterology2012，18，15： | 41 |
| 6 | Prognostic nutritional index predicts postoperative complications and long-term outcomes of gastric cancer显示文摘AIM:To investigate the impact of prognostic nutritional index(PNI)on the postoperative complications and long-term outcomes in gastric cancer patients undergoing total gastrectomy.METHODS:The data for 386 patients with gastric cancer were extracted and analyzed between January2003 and December 2008 in our center.The patients were divided into two groups according to the cutoff value of the PNI:those with a PNI≥46 and those with a PNI<46.Clinicopathological features were compared between the two groups and potential prognostic factors were analyzed.The relationship between postoperative complications and PNI was analyzed by logistic regression.The univariate and multivariate hazard ratios were calculated using the Cox proportional hazard model.RESULTS:The optimal cutoff value of the PNI was set at 46,and patients with a PNI≥46 and those with a PNI<46 were classified into PNI-high and PNI-low groups,respectively.Patients in the PNI-low group were more likely to have advanced tumor(T),node(N),and TNM stages than patients in the PNI-high group.The low PNI is an independent risk factor for the incidence of postoperative complications(OR=2.223).The 5-year overall survival(OS)rates were 54.1%and21.1%for patients with a PNI≥46 and those with a PNI<46,respectively.The OS rates were significantly lower in the PNI-low group than in the PNI-high group among patients with stagesⅡ(P=0.001)andⅢ(P<0.001)disease.CONCLUSION:The PNI is a simple and useful marker not only to identify patients at increased risk for postoperative complications,but also to predict long-term survival after total gastrectomy.The PNI should be included in the routine assessment of advanced gastric cancer patients. | Nan Jiang Jing-Yu Deng Xue-Wei Ding Bin Ke Ning Liu Ru-Peng Zhang Han Liang | 2014 | World Journal of Gastroenterology2014，20，30： | 41 |
| 7 | Characterizations and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping显示文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene. | YANG Qing-kai1,LIANG Chun-yang1,LI Jun1,JIN De-min1,AHUANG Wen2,DENG Qi-yun2,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101 2. Hunan Hybrid Rice Research Center,Changsha 410125) | 2007 | 湖南农业大学学报(自然科学版)2007，33，S1： | 34 |
| 8 | Clinical significance of lymph node metastasis in gastric cancer显示文摘Gastric cancer,one of the most common malignancies in the world,frequently reveals lymph node,peritoneum,and liver metastases.Most of gastric cancer patients present with lymph node metastasis when they were initially diagnosed or underwent surgical resection,which results in poor prognosis.Both the depth of tumor invasion and lymph node involvement are considered as the most important prognostic predictors of gastric cancer.Although extended lymphadenectomy was not considered a survival benefit procedure and was reported to be associated with high mortality and morbidity in two randomized controlled European trials,it showed significant superiority in terms of lower locoregional recurrence and disease related deaths compared to limited lymphadenectomy in a 15-year followup study.Almost all clinical investigators have reached a consensus that the predictive efficiency of the number of metastatic lymph nodes is far better than the extent of lymph node metastasis for the prognosis of gastric cancer worldwide,but other nodal metastatic classifications of gastric cancer have been proposed as alternatives to the number of metastatic lymph nodes for improving the predictive efficiency for patient prognosis.It is still controversial over whether the ratio between metastatic and examined lymph nodes is superior to the number of metastatic lymph nodes in prognostic evaluation of gastric cancer.Besides,the negative lymph node count has been increasingly recognized to be an important factor significantly associated with prognosis of gastric cancer. | Jing-Yu Deng Han Liang | 2014 | World Journal of Gastroenterology2014，20，14： | 32 |
| 9 | Efficacy and safety of a novel anti-HER2 therapeutic antibody RC48 in patients with HER2-overexpressing,locally advanced or metastatic gastric or gastroesophageal junction cancer:a single-arm phase II study显示文摘Background:Current treatment options for human epidermal growth factor receptor 2(HER2)-overexpressing gastric cancer at third-line have shown limited clinical benefit.Further,there is no specific treatment for HER2 immunohistochemistry(IHC)2+and fluorescence in-situ hybridization-negative patients.Here,we report the efficacy and safety of a novel anti-HER2 antibody RC48 for patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer.Methods:Patients with HER2-overexpressing(IHC 2+or 3+),locally advanced or metastatic gastric or gastroesophageal junction cancer who were under at least second-line therapy were eligible and received RC482.5 mg/kg alone every 2 weeks.The primary endpoint was the objective response rate(ORR)assessed by an independent review committee.Secondary endpoints included progressionfree survival(PFS),overall survival(OS),duration of response,time to progression,disease control rate,and safety.Results:Of 179 patients screened,125 were eligible and received RC48 treatment.The ORR was 24.8%(95%confidence interval[CI]:17.5%-33.3%).The median PFS and OS were 4.1 months(95%CI:3.7-4.9 months)and 7.9 months(95%CI:6.7-9.9 months),respectively.The most frequently reported adverse events were decreased white blood cell count(53.6%),asthenia(53.6%),hair loss(53.6%),decreased neutrophil count(52.0%),anemia(49.6%),and increased aspartate aminotransferase level(43.2%).Serious adverse events(SAEs)occurred in 45(36.0%)patients,and RC48-related SAEs were mainly decreased neutrophil count(3.2%).Seven patients had adverse events that led to death were not RC48-related.Conclusions:RC48 showed promising activity with manageable safety,suggesting potential application in patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer who have previously received at least two lines of chemotherapy. | Zhi Peng Tianshu Liu Jia Wei Airong Wang Yifu He Liuzhong Yang Xizhi Zhang Nanfeng Fan Suxia Luo Zhen Li Kangsheng Gu Jianwei Lu Jianming Xu Qingxia Fan Ruihua Xu Liangming Zhang Enxiao Li Yuping Sun Guohua Yu Chunmei Bai Yong Liu Jiangzheng Zeng Jieer Ying Xinjun Liang Nong Xu Chao Gao Yongqian Shu Dong Ma Guanghai Dai Shengmian Li Ting Deng Yuehong Cui Jianmin Fang Yi Ba Lin Shen | 2021 | Cancer Communications2021，41，11： | 29 |
| 10 | Validation of clinical significance of examined lymph node count for accurate prognostic evaluation of gastric cancer for the eighth edition of the American Joint Committee on Cancer(AJCC) TNM staging system显示文摘Objective: To validate the necessity of increasing the examined lymph node(ELN) count for enhancing the accuracy of prognostic evaluation of gastric cancer(GC) patients after curative gastrectomy in multiple medical centers of China.Methods: The clinicopathological data of 7,620 patients who underwent the curative resection for GC between2001 and 2011 were included to demonstrate whether the ELN count is indispensable for enhancing the accuracy of prognostic evaluation of GC patients after surgery. After a meticulous stratification by using the cut-point survival analysis, all included 7,620 patients were allocated into three groups as: less than 16(<16), between 16 and30(16-30), and more than 30(>30) ELNs. Survival differences among various subgroups of GC patients were analyzed to assess the impact of the ELN count on the stage migration in accordance with the overall survival(OS)of GC patients.Results: Survival analyses revealed that the ELN count was positively correlated with the OS(P=0.001) and was an independent prognostic predictor(P<0.01) of 7,620 GC patients. Stratum analysis showed that the accuracy of prognostic evaluation could be enhanced when the ELN count was no less than 16(≥16) for node-negative patients and >30 for node-positive patients. Stage migrations were mainly detected in the various subgroups of patients with specific pN stages as follows: pN0 with 16-30 ELNs(pN0_(16-30)) and pN0 with >30 ELNs(pN0_(>30)), pN0 with <16 ELNs(pN0_(<16)) and pN1_(>30), pN1_(<16) and pN2_(16-30), pN1_(16-30) and pN2_(>30), pN3a_(<16) and pN3b_(16-30), and pN3a_(<16) and pN3b_(>30). These findings indicate that increasing the ELN count is a prerequisite to guarantee precisely prognostic evaluation of GC patients.Conclusions: The ELN count should be proposed to be >30 for acquiring the accurate prognostic evaluation for GC patients, especially for node-positive patients. | Jingyu Deng Jinyuan Liu Wei Wang Zhe Sun Zhenning Wang Zhiwei Zhou Huimian Xu Han Liang | 2018 | Chinese Journal of Cancer Research2018，30，5： | 27 |
| 11 | Chronic hepatitis B serum promotes apoptotic damage in human renal tubular cells显示文摘瞄准:在肾的管状的上皮细胞在试管内的 apoptosis 上与长期的肝炎 B ( CHB )调查病人的浆液的效果并且在肝炎 B 的致病学习肝炎 B ( HBV )和转变生长因素贝它( 1 )(TGF贝它( 1 ))的角色病毒联系了肾小球性肾炎( HBV-GN )。方法:浆液 TGF 贝它(1 ) 的层次被特定的酶测量连接免疫吸着剂试金(ELISA ) 和 HBV DNA 被聚合酶链反应(PCR ) 作为控制与 CHB,和 20 个健康的人在 44 个病人测试。正常的人的肾近似管状的房间(HK-2 ) 和重量的单位是有教养的一 of 健康的人,有 HBV-DNA negative (20 个案例) 的 CHB 病人和 HBV-DNA 积极(24 个案例) 为多达 72 h。Apoptosis 和 HK-2 的船边交货表示被流动血细胞计数器检测。结果:HK-2 房间的 apoptosis 率和船边交货表达式在 HBV DNA 积极浆液组 19.01%+/-5.85% 和 17.58%+/-8.35% 是显著地更高的, HBV DNA 否定浆液在控制组 4.25%+/-0.65% 和 2.33%+/-1.09% 比那些组织 8.12%+/-2.80% 和 6.96%+/-2.76% ,分别地(P<0.01 ) 。在 HBV DNA 积极浆液组的 HK-2 的 apoptosis 率和船边交货表示比在 HBV DNA 否定浆液(P<0.01 ) 的那些显著地高。在 HBV DNA 积极浆液组的 HK-2 房间的 Apoptosis 率断然与 HBV-DNA 的水平被相关(r = 0.657 ) 。在 CHB 组的浆液 TGF 贝它(1 ) 的水平是 163.05+/-91.35 microg/L,象与在控制组(P<0.01 ) 的 81.40+/-40.75 microg/L 相比显著地更高。结论:有长期的肝炎 B 的病人的浆液由触发船边交货起来规定的一条小径在人的肾的管状的房间支持 apoptotic 损坏。HBV 和 TGF 贝它(1 ) 可以在肝炎 B 的机制起重要作用联系肾小球性肾炎。 | Cun-Liang Deng Xin-Wen song Hai-Jun Liang Chen Feng Yun-Jian Sheng Ming-Yong Wang | 2006 | World Journal of Gastroenterology2006，12，11： | 25 |
| 12 | A Genome-Wide Transcription Analysis Reveals a Close Correlation of Promoter INDEL Polymorphism and Heterotic Gene Expression in Rice Hybrids显示文摘杂种优势,或混合活力,指二个生来的变化的混合子孙在展出提高的生长或农学的性能的现象。尽管研究的世纪长的历史关于杂种优势的基因基础产生了几个假设,位于杂种优势和 heterotic 基因表示下面的分子的机制留下逃犯。这里,我们在米饭报导二个 heterotic 十字的染色体宽的基因表示分析,利用它的充分定序的染色体。约 79% 基因是从 heterotic 十字的二个集合在幼苗射击表示的差别,包括许多抄写因素基因,并且展出了基因行动的多重模式。在生来的父母之间的 ortholog 基因的通常认为的倡导者区域的比较揭示了广泛的顺序变化,特别地小的插入 / 删除(INDEL ) ,许多哪个在通常认为的 cis 规章的元素的形成 / 混乱的结果。一起,这些结果建议那在抄写因素和多态的倡导者的表示之间的组合相互影响在混血儿的 cis 规章的元素是位于 heterotic 基因行动下面的一嘴巧的分子的机制并且这样在米饭的杂种优势。 | Hui-Yong Zhang Hang He Liang-Bi Chen Lei Li Man-Zhong Liang Xiang-Feng Wang Xi-Gang Liu Guang-Ming He Run-Sheng Chen Li-Geng Ma Xing Wang Deng | 2008 | Molecular Plant2008，1，5： | 25 |
| 13 | Quantitative Analysis of Paleoatmospheric CO2 Level Based on Stomatal Characters of Fossil Ginkgo from Jurassic to Cretaceous in China显示文摘更好理论的 A 并且在地质的历史期间的在 paleo-CO_2 和气候之间的连接的实际理解是重要的提高现代人的社会的持续发展。自从 1980 年代,在植物生理学的开发导致了石块植物能为 paleoatmosphere 和 paleobiosphere 作为一个代理服务的实现。作为有进化壅滞的寡妇裸子植物,白果树很好适合 paleoenvironmental 重建。这篇论文用植物生理学的解剖数据在中国的北方从综合阶层分析石块白果树种类。用有气孔的参数,为 paleo-C0_2 水平的一个趋势在早中间的侏罗记和早白垩纪期间被获得,它与由 GEOCARB 的估计一致。趋势也类似于在地质的时间的吝啬的全球表面温度的。与三个另外的大气的 CO_2 集中参数相比, paleo-CO_2 水平的趋势基于从三个连续阶层的石块白果树标本的有气孔的参数是更多准确。 | SUN Bainian XIAO Liang XIE Sanping DENG Shenghui WANG Yongdong JIA Hui Susan Tumer | 2007 | Acta Geologica Sinica(English Edition)2007，81，6： | 23 |
| 14 | Genome-wide comparative analysis of type-A Arabidopsis response regulator genes by overexpression studies reveals their diverse roles and regulatory mechanisms in cytokinin signaling显示文摘细胞激动素是为植物生长和开发的各种各样的方面的一个批评生长管理者。在 Arabidopsis,细胞激动素发信号被从受体播送一个信号的二部件的基于系统的 phosphorelay 调停,通过 histidine phosphotransfer 蛋白质,到下游的反应管理者(ARR ) 。这些 ARR,打字 -- A ARR 基因,其抄写能被细胞激动素很快导致,充当细胞激动素发信号的否定管理者。然而因为功能的冗余性,类型的功能 -- 在植物生长和开发的 A ARR 基因没被分析 loss-of-function 异种很好理解。在这研究,我们在所有十种类型上执行了比较功能的研究 -- 由分析这些 ARR 基因熔化了到 MYC epitope 的转基因的植物 overexpressing 的 A ARR 基因标注。ARR 基因的 Overexpression 导致许多联系细胞激动素的显型。尤其是,不同 ARR transgenes 的 overexpression 引起多样的显型,甚至在种系发生地密切相关的基因对之间,例如在 ARR3-ARR4 和 ARR5-ARR6 对以内。我们发现了 ARR 蛋白质的一个子集的累积(ARR3, ARR5, ARR7, ARR16 和 ARR17;可能 ARR8 和 ARR15 ) 被 MG132 增加,一个特定的 proteasomal 禁止者,显示这些蛋白质的那稳定性被 proteasomal 调整降级。而且,类似于的以前描绘的 ARR5, ARR6 和 ARR7, ARR16 和 ARR17 的稳定性,可能包括的 ARR8 和 ARR15,被细胞激动素调整。这些结果建议那种类型 -- A ARR 蛋白质被包含细胞激动素和 proteasome 小径的组合机制调整,从而在植物生长和开发执行特殊函数。 | Bo Ren Yan Liang Yan Deng Qingguo Chen Jian Zhang Xiaohui Yang Jianru Zuo | 2009 | Cell Research2009，19，10： | 23 |
| 15 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 16 | Berberine Ameliorates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Rats via Activation of SIRT3/AMPK/ACC Pathway显示文摘This study aimed to verify the effects of berberine(BBR)on the fat metabolism proteins involved in the sirtuin 3(SIRT3)/adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)pathway in the liver tissues of rats with high-fat diet(HFD)-induced non-alcoholic fatty liver disease(NAFLD).Forty-eight rats were randomly divided into the normal control(NC)group,HFD group or BBR group,with 16 rats in each group.After 8 and 16 weeks of treatment,serum and liver samples were collected.Subsequently,body parameters,biochemical parameters and liver pathology were examined.The expression levels of proteins involved in the SIRT3/AMPK/ACC pathway in the liver were detected by Western blotting.After 8 and 16 weeks of a HFD,the successful establishment of rat models with different degrees of NAFLD was confirmed by hematoxylin and eosin(H&E)and Oil Red O staining.NAFLD rat models exhibited obesity and hyperlipidemia,and the protein expression levels of SIRT3,p-AMPK.p-ACC,and CPT-1A in the liver were significantly decreased compared to those in the NC group.The concurrent administration of BBR with the HFD effectively improved serum and liver lipid profiles and ameliorated liver injury.Furthermore,the protein expression levels of SIRT3,p-AMPK,p-ACC,and CPT-1 A in the liver were significantly increased in the BBR group as compared with those in the HFD group.In conclusion,our data suggest that the mechanism by which BBR ameliorates HFD-induced hepatic steatosis may be related to the activation of the SIRT3/AMPK/ACC pathway in the liver. | Yu-pei ZHANG Yuan-jun DENG Kai-rui TANG Run-sen CHEN Shu LIANG Yin-ji LIANG Li HAN Ling JIN Zi-en LIANG Yan-ning CHEN Qin-he YANG | 2019 | Current Medical Science2019，39，1： | 21 |
| 17 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 18 | Portosplenomesenteric vein thrombosis in patients with early-stage severe acute pancreatitis显示文摘AIM To investigate the incidence and risk factors of portosplenomesenteric vein thrombosis(PSMVT) in the early stage of severe acute pancreatitis(SAP).METHODS Patients with SAP in a tertiary care setting from January 2014 to December 2016 were retrospectively reviewed. All contrast-enhanced computed tomography(CT) studies were reassessed and reviewed. Clinical outcome measures were compared between SAP patients with and without PSMVT in the early stage of the disease. Univariate and multivariate logistic regression analyses were sequentially performed to assess potential risk factors for the development of PSMVT in SAP patients. A receiver operating characteristic(ROC) curve was generated for the qualifying independent risk factors.RESULTS Twenty-five of the one hundred and forty(17.86%) SAP patients developed PSMVT 6.19 ± 2.43 d after acute pancreatitis(AP) onset. PSMVT was confirmed by contrast-enhanced CT. Multivariate stepwise logistic regression analyses showed that Balthazar's CT severity index(CTSI) scores [odds ratio(OR): 2.742; 95% confidence interval(CI): 1.664-4.519; P = 0.000], hypoalbuminemia(serum albumin level < 25 g/L)(OR: 32.573; 95%CI: 2.711-391.353; P = 0.006) and gastrointestinal wall thickening(OR: 4.367, 95%CI: 1.218-15.658; P = 0.024) were independent risk factors for PSMVT developed in patients with SAP. The area under the ROC curve for Balthazar's CTSI scores was 0.777(P = 0.000), the sensitivity was 52%, and the specificity was 93% at a cut-off value of 5.5.CONCLUSION High Balthazar's CTSI scores, hypoalbuminemia and gastrointestinal wall thickening are independent risk factors for PSMVT developed in the early stage of SAP. | Ling Ding Feng Deng Chen Yu Wen-Hua He Liang Xia Mi Zhou Xin Huang Yu-Peng Lei Xiao-Jiang Zhou Yin Zhu Nong-Hua Lu | 2018 | World Journal of Gastroenterology2018，24，35： | 20 |
| 19 | Characteristics and prognosis of gastric cancer in patients aged ≥ 70 years显示文摘AIM:To elucidate the prognostic value of age for gastric cancer and identify the optimal treatment for elderly gastric cancer patients.METHODS:We enrolled 920 patients with gastric cancer who underwent gastrectomy between January 2003and December 2007 in our center.Patients were categorized into three groups:younger group(age<50years),middle-aged group(50-69 years),and elderly group(≥70 years).Clinicopathological features were compared among the three groups and potential prognostic factors were analyzed.The log-rank test was used to assess statistical differences between curves.Independent prognostic factors were identified by the Cox proportional hazards regression model.Stratified analysis was used to investigate the impact of age on survival at each stage.Cancer-specific survival was also compared among the three groups by excluding deaths due to reasons other than gastric cancer.We analyzed the potential prognostic factors for patients aged≥70years.Finally,the impact of extent of lymphadenectomy and postoperative chemotherapy on survival for each age group was evaluated.RESULTS:In the elderly group,there was a male predominance.At the same time,cancers of the upper third of the stomach,differentiated type,and less-invasive surgery were more common than in the younger or middle-aged groups.Elderly patients were more likely to have advanced tumor-node-metastasis(TNM)stage and larger tumors,but less likely to have distant metastasis.Although 5-year overall survival(OS)rate specific to gastric cancer was not significantly different among the three groups,elderly patients demonstrated a significantly lower 5-year OS rate than the younger and middle-aged patients(elderly vs middle-aged vs younger patients=22.0%vs 36.6%vs 38.0%,respectively).In the TNM-stratified analysis,the differences in OS were only observed in patients withⅡandⅢtumors.In multivariate analysis,only surgical margin status,pT4,lymph node metastasis,M1 and sex were independent prognostic factors for elderly patients.The5-year OS rate did not differ between elderly patients undergoing D1 and D2 lymph node resection,and these patients benefited little from chemotherapy.CONCLUSION:Age≥70 years was an independent prognostic factor for gastric cancer after gastrectomy.D1 resection is appropriate and postoperative chemotherapy is possibly unnecessary for elderly patients with gastric cancer. | Yue-Xiang Liang Jing-Yu Deng Han-Han Guo Xue-Wei Ding Xiao-Na Wang Bao-Gui Wang Li Zhang Han Liang | 2013 | World Journal of Gastroenterology2013，19，39： | 20 |
| 20 | A modified single-armed technique for microsurgical vasoepididymostomy显示文摘这研究是与 epididymal 在病人为 microsurgical vasoepididymostomy (VE ) 评估一种修改武装单人赛的缝术技术的有效性妨碍的精子缺乏。从 2011 年 9 月到 2011 年 12 月, microsurgical 二缝术的纵的摄取 VE 与 epididymal 在 17 个人用我们的修改武装单人赛的缝术技术被执行在我们的医院的妨碍的精子缺乏。二个这些病人在以前的没有通过的 VE 以后经历了重复 VE,并且因为左腹的管 deferens 的吸藏,一个病人经历了单方的 VE。在在 3 月 postoperation 的精液样品的精子的存在被用作这研究的初步的端点。每个病人在 3 月的时间点提供了至少一件精液样品,并且明显被精子的再现估计(>在精液的 10 4 ml −1) 。修改技术的吝啬的起作用的时间是 219 min。明显在 10 个人被注意(58.8 %) ,包括经历了重复 VE 的一个病人。经历了单方的吻合的病人没在他的精液手术后地表明精子。精子 granulomas 没在这个队被检测。这研究的结果表明那我们为 microsurgical 的修改技术纵的摄取 VE 有效。我们相信是一种实际选择可以减少操作时间并且排除缝术十字路口。 | Liang Zhao Chun-Hua Deng Xiang-Zhou Sun Yu Chen Wen-Wei Wang Liang-Yun Zhao Ling-You Zeng Xiang-An Tu | 2013 | Asian Journal of Andrology2013，15，1： | 19 |
您所在的机构暂未开通此部分的功能，请联系管理员