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| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 3 | Malate transported from chloroplast to mitochondrion triggers production of ROS and PCD in Arabidopsis thaliana显示文摘规划房间死亡(PCD ) 是一个基本生物过程。在马赛克死亡 1 的缺乏(MOD1 ) ,质体局部性的 enoyl-ACP reductase,导致反应的氧种类(ROS ) 和 PCD 的累积,它能被 mitochondrial 建筑群压制我变化,从叶绿体显示一个信号到线粒体。然而,这个信号尚待被阐明。在这研究,通过克隆并且分析一系列 mod1 suppressors,我们揭示调整 mitochondrial ROS 和扳机 PCD 的产生的一条全面细胞器通讯小径。我们证明在 PLASTIDIAL NAD 依赖的 MALATE 脱氢酶(plNAD-MDH ) 的变化, chloroplastic DICARBOXYLATE TRANSPORTER 1 (DiT1 ) 并且(mMDH1 ) MITOCHONDRIAL MALATE 脱氢酶 1 能各在 mod1 救 ROS 累积和 PCD 显型,经由 malate 梭从叶绿体表明直接通讯到线粒体。进一步的研究证明这些元素在不同光周期条件下面在氧化还原作用动态平衡和植物生长起关键作用。而且,我们表明 ROS 水平和 PCD 显著地在房间,它能是戏剧性地稀释了由的对待 malate 的 HeLa 被增加人的基因 MDH2 击倒, Arabidopsis mMDH1 的 ortholog。这些结果揭开在植物和动物系统的一条保存导致 malate 的 PCD 小径,革命化我们在细胞器之间的通讯的理解。 | Yannan Zhao Lilan Luo Jiesi Xu Peiyong Xin Hongyan Guo Jian Wu Lin Bai Guodong Wang Jinfang Chu Jianru Zuo Hong Yu Xun Huang Jiayang Li | 2018 | Cell Research2018，28，4： | 24 |
| 4 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 5 | Rice Expression Database(RED):An integrated RNA-Seq-derived gene expression database for rice显示文摘Rice is one of the most important stable food as well as a monocotyledonous model organism for the plant research community.Here,we present RED(Rice Expression Database;http://gffzzd1bc3ab4bbbc4005hobpopkxv5kpu6wxk.ffgz.tsg.suse.edu.cn),an integrated database of rice gene expression profiles derived entirely from RNA-Seq data.RED features a comprehensive collection of 284 high-quality RNA-Seq experiments,integrates a large number of gene expression profiles and covers a wide range of rice growth stages as well as various treatments.Based on massive expression profiles,RED provides a list of housekeeping and tissue-specific genes and dynamically constructs co-expression networks for gene(s) of interest.Besides,it provides user-friendly web interfaces for querying,browsing and visualizing expression profiles of concerned genes.Together,as a core resource in BIG Data Center,RED bears great utility for characterizing the function of rice genes and better understanding important biological processes and mechanisms underlying complex agronomic traits in rice. | Lin Xia Dong Zou Jian Sang Xingjian Xu Hongyan Yin Mengwei Li Shuangyang Wu Songnian Hu Lili Hao Zhang Zhang | 2017 | Journal of Genetics and Genomics2017，44，5： | 12 |
| 6 | Response of runoff to climate change and its future tendency in the source region of Yellow River显示文摘这研究检验水文学和来源区域的气象学的数据从 1956 ~ 2010 的黄河和从地区性的气候的未来气候情形当模特儿(摘要) 在 20102020 期间。通过分析流动变化并且揭示气候原因,它为未来流动预言变化趋势。年度吝啬的流动显示出一个减少的趋势在,这被发现最近在有 5a, 8a, 15a, 22a 和 42a 的伪时期的黄河的来源区域的 50 年;削弱的华南海夏天季风导致了降水减少,以及蒸发增加并且在全球温暖的情形冻结土壤退化是气候因素,它引起了流动减少。基于地区性的气候模型摘要预言,在黄河的来源区域的流动是可能的在下一 20 年里通常减少。 | Li Lin Shen Hongyan Dai Sheng Xiao Jianshe Shi Xinghe | 2012 | Journal of Geographical Sciences2012，22，3： | 11 |
| 7 | Tribological properties of copper matrix composites reinforced with homogeneously dispersed graphene nanosheets显示文摘Graphene reinforced copper matrix composites(Gr/Cu) were fabricated by electrostatic self-assembly and powder metallurgy. The morphology and structure of graphene oxide, graphene oxide-Cu powders and Gr/Cu composites were characterized by scanning electronic microscopy, transmission electronic microscopy, X-ray diffraction and Raman spectroscopy, respectively. The effects of graphene contents,applied loads and sliding speeds on the tribological behavior of the composites were investigated. The results indicate that the coefficient of friction of the composites decreases first and then increases with increasing the graphene content. The lowest friction coefficient is achieved in 0.3 wt% Gr/Cu composite,which decreases by 65% compared to that of pure copper. The coefficient of friction of the composite does not have significant change with increasing the applied load, however, it increases with increasing the sliding speed. The tribological mechanisms of the composite under different conditions were also investigated. | Xin Gao Hongyan Yue Erjun Guo Shaolin Zhang Longhui Yao Xuanyu Lin Bao Wang Enhao Guan | 2018 | Journal of Materials Science & Technology2018，34，10： | 9 |
| 8 | Joint utilization of genetic analysis and semi-cloning technology reveals a digenic etiology of Müllerian anomalies显示文摘Dear Editor,Identifying pathogenic gene mutations and their combination is critical but challenging in dissecting the etiology of complex diseases when more than one gene is involved.1 The digenic/oligogenic/omnigenic models,holding that more than one gene could act synergistically,appeal to a wide range of genes responsible for complex phenotypes.1,2,3,4 These genetic models advanced our understanding of genetic factors underlying complex phenotypes,yet an accordingly rapid and efficient experimental assay for identifying pathogenic combinations of genetic variants at animal model level is lacking and urgently needed. | Lingbo Wang Ying Zhang Xiaoyi Fu Shuangshuang Dong Shuyan Tang Ning Zhang Chengcheng Song Nan Yang Lin Zhang Hongyan Wang Huijuan Shi Li Jin Feng Zhang Jinsong Li Keqin Hua | 2020 | Cell Research2020，30，1： | 8 |
| 9 | Whole genome sequencing of 10K patients with acute ischaemic stroke or transient ischaemic attack: design, methods and baseline patient characteristics显示文摘Background and purpose Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China,but its underlying risk genes and pathways are far from being comprehensively understood.We here describe the design and methods of whole genome sequencing(WGS)for 10914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry(CNSR-III).Methods Baseline clinical characteristics of the included patients in this study were reported.DNA was extracted from white blood cells of participants.Libraries are constructed using qualified DNA,and WGS is conducted on BGISEQ-500 platform.The average depth is intended to be greater than 30×for each subject.Afterwards,Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants(SNVs)and insertion-deletions.For each included subject,21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual.The copy number variations and structural variations are also called for each patient.All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases.Results The average age of the included 10914 patients was 62.2±11.3 years,and 31.4%patients were women.Most of the baseline clinical characteristics of the 10914 and the excluded patients were balanced.Conclusions The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke. | Si Cheng Zhe Xu Yang Liu Jinxi Lin Yong Jiang Yilong Wang Xia Meng Anxin Wang Xinying Huang Zhimin Wang Guohua Chen Songdi Wu Zhengchang Jia Yongming Chen Xuerong Qiu Jun Wu Binbin Song Weizhong Ji Zhongping An Wenjun Xue Lili Zhao Yu Geng Hongyan Li Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 8 |
| 10 | Promoter methylation of Wnt/β-Catenin signal inhibitor TMEM88 is associated with unfavorable prognosis of nonsmall cell lung cancer显示文摘Objective:Recent research has indicated that altered promoter methylation of oncogenes and tumor suppressor genes is an important mechanism in lung cancer development and progression.In this study,we investigated the association between promoter methylation of TMEM88,a possible inhibitor of the Wnt/β-Catenin signaling,and the survival of patients with nonsmall cell lung cancer(NSCLC).Methods:Twelve pairs of tumor and adjacent non-tumor samples were used for microarray analyses of DNA methylation and gene expression.For validation,more than two hundred additional samples were analyzed for methylation using bisulfite pyrosequencing and for gene expression using q RT-PCR.Then the cell function were tested by wound healing,transwell,CCK8 and cell cycle assay.Results:Our analysis of patient specimens showed that TMEM88 methylation was higher in NSCLC tumors(82.2%±10.3,P<0.01)compared with the adjacent normal tissues(65.9%±7.2).The survival analysis revealed that patients with high TMEM88methylation had a shorter overall survival(46 months)compared with patients with low TMEM88 methylation(>56 months;P=0.021).In addition,we found that demethylation treatment could inhibit tumor cell proliferation,migration,and invasion,which was supportive of an association between methylation and survival.Conclusions:Based on these consistent observations,we concluded that TMEM88 may play an important role in NSCLC progression and that promoter methylation of TMEM88 may serve as a biomarker for NSCLC prognosis and treatment. | Rongna Ma Nannan Feng Xiao Yu Hongyan Lin Xiaohong Zhang Oumin Shi Huan Zhang Shuo Zhang Lei Li Min Zheng Ming Gao Herbert Yu Biyun Qian | 2017 | Cancer Biology & Medicine2017，14，4： | 6 |
| 11 | TXNDC5 synergizes with HSC70 to exacerbate the inflammatory phenotype of synovial fibroblasts in rheumatoid arthritis through NF-κB signaling显示文摘The upregulated expression of thioredoxin domain-containing protein 5(TXNDC5)is associated with rheumatoid arthritis in patients and model mice.However,the underlying mechanism by which TXNDC5 influences the pathological activation of rheumatoid arthritis synovial fibroblasts(RASFs)remains unknown.In this study,we show that TXNDC5 expression in RASFs and their cytokine production are significantly upregulated in response to LPS,TNF-αand IL-6,but suppressed by transfection with TXNDC5-siRNA.TXNDC5 is further validated as the direct target of NF-κB signaling.Mechanistically,TXNDC5 directly interacts with heat shock cognate 70 protein(HSC70)to sequester it in the cytoplasm,and HSC70 silencing exerts the same effects as TXNDC5 on the biological activity of RASFs(for example,decreased cell viability,invasion and cytokine production).Furthermore,HSC70 activates NF-κB signaling by destabilizing IκBβprotein in the absence of LPS or facilitating its nuclear translocation in the presence of LPS.Importantly,TXNDC5 can also regulate the activity of NF-κB signaling in a HSC70-IκBβ-dependent manner.Taken together,by linking HSC70 and NF-κB signaling,TXNDC5 plays a pro-inflammatory role in RASFs,highlighting a potential approach to treat RA by blocking the TXNDC5/HSC70 interaction. | Lin Wang Hongyan Dong Guanhua Song Rui Zhang Jihong Pan Jinxiang Han | 2018 | Cellular & Molecular Immunology2018，15，7： | 5 |
| 12 | Regulation of mitochondrial NAD pool via NAD^+ transporter 2 is essential for matrix NADH homeostasis and ROS production in Arabidopsis显示文摘Reactive oxygen species(ROS) play a crucial role in numerous biological processes in plants, including development, responses to environmental stimuli, and programmed cell death(PCD). Deficiency in MOSAIC DEATH 1(MOD1), a plastid-localized enoyl-ACP reductase essential for de novo fatty acid biosynthesis in Arabidopsis thaliana, leads to the increased malate export from chloroplasts to mitochondria, and the subsequent accumulation of mitochondria-generated ROS and PCD. In this study, we report the identification and characterization of a mod1 suppressor, som592. SOM592 encodes mitochondrion-localized NAD^+ transporter 2(NDT2). We show that the mitochondrial NAD pool is elevated in the mod1 mutant. The som592 mutation fully suppressed mitochondrial NADH hyper-accumulation, ROS production, and PCD in the mod1 mutant, indicating a causal relationship between mitochondrial NAD accumulation and ROS/PCD phenotypes. We also show that in wild-type plants, the mitochondrial NAD+uptake is involved in the regulation of ROS production in response to continuous photoperiod. Elevation of the alternative respiration pathway can suppress ROS accumulation and PCD in mod1, but leads to growth restriction. These findings uncover a regulatory mechanism for mitochondrial ROS production via NADH homeostasis in Arabidopsis thaliana that is likely important for growth regulation in response to altered photoperiod. | Lilan Luo Yajun He Yannan Zhao Qian Xu Jian Wu Haiyan Ma Hongyan Guo Lin Bai Jianru Zuo Jian-Min Zhou Hong Yu Jiayang Li | 2019 | Science China(Life Sciences)2019，62，8： | 5 |
| 13 | Knockdown of long non-coding RNA LCPAT1 inhibits autophagy in lung cancer显示文摘Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcinomas, and termed it lung cancer progression-associated transcript 1(LCPAT1). To characterize the role of LCPAT1 in lung cancer, we conducted the current study.Methods: Expression of LCPAT1 and autophagy-associated markers in tumor tissues and lung cancer cell lines was determined by real-time quantitative polymerase chain reaction(q PCR). Hematoxylin and eosin(HE) staining, q PCR, Western blot, and immunohistochemistry were performed to evaluate xenografted tumor tissues. Autophagy induced by rapamycin was detected by Western blot and immunofluorescence in lung cancer cell lines.Results: Expression of LCPAT1 and microtubule-associated protein 1 light chain 3 beta(LC3B) was positively correlated in lung cancer. Knockdown of LCPAT1 inhibited tumor growth and suppressed cell autophagy in vivo. Moreover, LCPAT1 knockdown in lung cancer cell lines resulted in decreased autophagy-associated gene expression and alleviated the cell autophagy induced by rapamycin.Conclusions: We speculate that LCPAT1 plays a crucial role in regulating autophagy in lung cancer. | Xiao Yu Xiaofei Ye Hongyan Lin Nannan Feng Sumeng Gao Xiaohong Zhang Yu Wang Herbert Yu Xiaobei Deng Biyun Qian | 2018 | Cancer Biology & Medicine2018，15，3： | 5 |
| 14 | Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p显示文摘Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as exerkines.However,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.Methods:A 9-day treadmill training was used as an exercise model in C57BL/6 mice.Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in vitro.Results:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro.Exercise increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in mice.Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in mice.Mechanistically,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.Conclusion:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair. | Jing Lou Jie Wu Mengya Feng Xue Dang Guiling Wu Hongyan Yang Yan Wang Jia Li Yong Zhao Changhong Shi Jiankang Liu Lin Zhao Xing Zhang Feng Gao | 2022 | Journal of Sport and Health Science2022，11，4： | 4 |
| 15 | Efficient CO_2 capture on low-cost silica gel modified by polyethyleneimine显示文摘In this work,a series of polyethyleneimine(PEI) functionalized commercial silica gel were prepared by wet impregnation method and used as CO 2 sorbent.The as-prepared sorbents were characterized by N 2 adsorption,FT-IR and SEM techniques.CO 2 capture was tested in a fixed bed reactor using a simulated flue gas containing 15.1% CO 2 in a temperature range of 25-100 C.The effects of sorption temperature and amine content on CO 2 uptake of the adsorbents were investigated.The silica gel with a 30 wt% PEI loading manifested the largest CO 2 uptake of 93.4 mg CO 2 /g adsorbent(equal to 311.3 mg CO 2 /g PEI) among the tested sorbents under the conditions of 15.1%(v/v) CO 2 in N 2 at 75 C and atmospheric pressure.Moreover,it was rather low-cost.In addition,the PEI-impregnated silica gel exhibited stable adsorption-desorption behavior during 5 consecutive test cycles.These results suggest that the PEI-impregnated silica gel is a promising and cost-effective sorbent for CO 2 capture from flue gas and other stationary sources with low CO 2 concentration. | Ke Wang Hongyan Shang Lin Li Xinlong Yan Zifeng Yan Chenguang Liu Qingfang Zha | 2012 | Journal of Natural Gas Chemistry2012，21，3： | 4 |
| 16 | Telomere-to-telomere and gap-free reference genome assembly of the kiwifruit Actinidia chinensis显示文摘Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C.However,the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regions.Here,we report a highly continuous and completely gap-free reference genome of Actinidia chinensis cv.‘Hongyang’,named Hongyang v4.0,which is the first to achieve two de novo haploid-resolved haplotypes,HY4P and HY4A.HY4P and HY4A have a total length of 606.1 and 599.6 Mb,respectively,with almost the entire telomeres and centromeres assembled in each haplotype.In comparison with Hongyang v3.0,the integrity and contiguity of Hongyang v4.0 is markedly improved by filling all unclosed gaps and correcting some misoriented regions,resulting in∼38.6–39.5 Mb extra sequences,which might affect 4263 and 4244 protein-coding genes in HY4P and HY4A,respectively.Furthermore,our gap-free genome assembly provides the first clue for inspecting the structure and function of centromeres.Globally,centromeric regions are characterized by higher-order repeats that mainly consist of a 153-bp conserved centromere-specific monomer(Ach-CEN153)with different copy numbers among chromosomes.Functional enrichment analysis of the genes located within centromeric regions demonstrates that chromosome centromeres may not only play physical roles for linking a pair of sister chromatids,but also have genetic features for participation in the regulation of cell division.The availability of the telomere-to-telomere and gap-free Hongyang v4.0 reference genome lays a solid foundation not only for illustrating genome structure and functional genomics studies but also for facilitating kiwifruit breeding and improvement. | Junyang Yue Qinyao Chen Yingzhen Wang Lei Zhang Chen Ye Xu Wang Shuo Cao Yunzhi Lin Wei Huang He Xian Hongyan Qin Yanli Wang Sijia Zhang Ying Wu Songhu Wang Yi Yue Yongsheng Liu | 2023 | Horticulture Research2023，10，2： | 4 |
| 17 | Entinostat,a classⅠselective histone deacetylase inhibitor,plus exemestane for Chinese patients with hormone receptor-positive advanced breast cancer:A multicenter,randomized,double-blind,placebo-controlled,phase 3 trial显示文摘Entinostat plus exemestane in hormone receptor-positive(HR+)advanced breast cancer(ABC)previously showed encouraging outcomes.This multicenter phase 3 trial evaluated the efficacy and safety of entinostat plus exemestane in Chinese patients with HR+ABC that relapsed/progressed after≥1 endocrine therapy.Patients were randomized(2:1)to oral exemestane 25 mg/day plus entinostat(n=235)or placebo(n=119)5 mg/week in 28-day cycles.The primary endpoint was the independent radiographic committee(IRC)-assessed progression-free survival(PFS).The median age was 52(range,28—75)years and 222(62.7%)patients were postmenopausal.CDK4/6 inhibitors and fulvestrant were previously used in 23(6.5%)and 92(26.0%)patients,respectively.The baseline characteristics were comparable between the entinostat and placebo groups.The median PFS was 6.32(95%CI,5.30—9.11)and 3.72(95%CI,1.91—5.49)months in the entinostat and placebo groups(HR,0.76;95%CI,0.58—0.98;P=0.046),respectively.Grade≥3 adverse events(AEs)occurred in 154(65.5%)patients in the entinostat group versus 23(19.3%)in the placebo group,and the most common grade≥3 treatment-related AEs were neutropenia[103(43.8%)],thrombocytopenia[20(8.5%)],and leucopenia[15(6.4%)].Entinostat plus exemestane significantly improved PFS compared with exemestane,with generally manageable toxicities in HR+ABC(ClinicalTrials.gov#NCT03538171). | Binghe Xu Qingyuan Zhang Xichun Hu Qing Li Tao Sun Wei Li Quchang Ouyang Jingfen Wang Zhongsheng Tong Min Yan Huiping Li Xiaohua Zeng Changping Shan Xian Wang Xi Yan Jian Zhang Yue Zhang Jiani Wang Liang Zhang Ying Lin Jifeng Feng Qianjun Chen Jian Huang Lu Zhang Lisong Yang Ying Tian Hongyan Shang | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 3 |
| 18 | miR-573 is a negative regulator in the pathogenesis of rheumatoid arthritis显示文摘风湿性关节炎(RA ) 是反常发炎, angiogenesis,和软骨破坏描绘的长期的自体免疫的疾病。我们的以前的学习在 RA 的 synovial 纸巾表明了包含 5 的 thioredoxin 领域(TXNDC5 ) 的增加的表情,并且它的 overexpression 在 RA 病理被含有。尽管 TXNDC5 变化被连接到基因危险性到 RA,它的反常表示的规定一直不是明确的。这里,我们证明 TXNDC5 被 microRNA (米尔) 直接指向接着, -573, 和 TXNDC5 调停 RA (RASF ) 的 synovial 成纤维细胞的侵略上的 miR-573 的镇压效果。miR-573 overexpression 压制了 interleukin 的表示 6 (IL-6 ) 并且 cyclooxygenase 2 在由响应 lipopolysaccharide (LPS ) 的激活的 THP-1 房间的肿瘤坏死 factor-alpha 和 interleukin-1 贝它的 RASF,以及生产刺激。而且,有有 miR-573 的 RASF transfected 的调节媒介的处理模仿禁止了人的脐的静脉 endothelial 房间(HUVEC ) 的 angiogenic 能力。笔记,表皮的生长因素受体和像使用费的受体, 2 作为 miR-573 的新直接目标被验证,并且调停象 HUVEC 的 angiogenesis 一样的 IL-6 生产上的 miR-573 的规定。另外,外长的 miR-573 表示压制了激活 mitogen 的蛋白质的激活 kinase (MAPK ) ,信号变换器和抄写 3 的使活跃之物,和在响应 LPS 的 RASF 的 phosphatidylinositol-3 kinase/activate 蛋白质 kinase B。确实, MAPK 发信号是必要的保证 miR-573 的功能。一起拿,我们的学习在 RA 的病理学的过程向 miR-573 的保护的角色指并且在 RA 的处理建议一个潜在的目标。 | Lin Wang Guanhua Song Yabing Zheng Dan Wang Hongyan Dong Jihong Pan Xiaotian Chang | 2016 | Cellular & Molecular Immunology2016，13，6： | 3 |
| 19 | Combined detection and subclass characteristics analysis of CTCs and CTECs by SE-iFISH in ovarian cancer显示文摘Objective:Hematogenous metastasis is essential for the progression of ovarian cancer(OC),and circulating tumor cells(CTCs)are part of the metastatic cascade.However,the detection rate of CTC is low due to the use of less sensitive detection methods.Therefore,this study aimed to detect CTCs and circulating tumorigenic endothelial cells(CTECs)in patients with OC using subtraction enrichment and immunostaining and fluorescence in situ hybridization(SE-iFISH).Methods:We enrolled a total of 56 subjects,including 20 OC patients and 36 ovarian benign tumor patients.CTCs and CTECs were captured by subtraction enrichment(SE)and counted and classified according to immunofluorescence staining of tumor markers(TMs)carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)combined with fluorescence in situ hybridization(iFISH)of chromosome 8(Chr8)aneuploidy.The diagnostic value and subtype characteristics of CTCs and CTECs were investigated.Results:The detection rate of CTCs by SE-iFISH was high.Compared with CA125 and HE4,Chr8 aneuploidy was the major identification feature of CTC.CTC counts in OC were statistically higher than those in benign groups.CTC and CTEC with≥pentaploidy were detected in both groups,illustrating the poor diagnostic value of CTC or CTEC.Distributions of triploid and tetraploid CTC subtypes were significantly different,and combined detection of triploid and tetraploid CTCs showed the best diagnostic value.In contrast,the distribution of CTECs in the OC and benign groups had no statistically significant difference.Small CTCs accounted for over 1/3 of the total CTC count.We also found that small CTCs and CTECs primarily comprised triploid cells,while large CTCs and CTECs mainly comprised pentaploidy and beyond.Conclusions:The application of SE-iFISH offered a more comprehensive understanding of heterogeneous CTCs and CTECs in OC.Analysis of subclass characteristics of the CTCs and CTECs according to Chr8 aneuploidy and cell size may broaden their potential clinical utility and deepen mechanistic studies in OC. | Hongyan Cheng Shang Wang Wenqing Luan Xue Ye Sha Dou Zhijian Tang Honglan Zhu Peter Ping Lin Yi Li Heng Cui Xiaohong Chang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 3 |
| 20 | Inhibitory Effects of Ginseng Water Extract on Hydroxyl Radical显示文摘[Objectives] The aim of this work was to compare the inhibitory effects of different water-soluble extracts of ginseng on hydroxyl radical. [Methods]The water-soluble effective parts of ginseng were extracted by orthogonal test,the hydroxyl radical scavenging rate of each extract was determined by salicylic acid method,and the extraction process of the optimum hydroxyl radical inhibitor was optimized using variance analysis and cluster analysis. [Results] A total of 16 samples were prepared. Among them,the sample No. 12 had the highest hydroxyl radical scavenging rate,and the inhibition rate reached 105. 08% at the concentration of 1 g/L. [Conclusions] Ginseng water extract contained hydroxyl radical scavenger,of which the optimum extraction process was as follows: p H of 8,solid to liquid ratio of 1∶ 10,extraction temperature of 4℃,extraction time of 12 h and extraction times of 3. | Hongyan LI Ruihai LI Lin ZHAO Yang CAO | 2018 | Medicinal Plant2018，9，4： | 3 |
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