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| 1 | Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant显示文摘 | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 46 |
| 2 | A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice显示文摘Dear Editor,Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in both plant molecular biology research and crop improvement,of which generating targeted point mutation for gain-of-function phenotype is of great value.Although tremendous efforts have been made in developing geneinsertion or replacement approaches by | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Honghui Lin Huanbin Zhou | 2017 | Science China(Life Sciences)2017，60，5： | 28 |
| 3 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 4 | Ultrasensitive skin‐like wearable optical sensors based on glass micro/nanofibers显示文摘Electronic skin,a class of wearable electronic sensors that mimic the functionalities of human skin,has made remarkable success in applications including health monitoring,human-machine interaction and electronic-biological interfaces.While electronic skin continues to achieve higher sensitivity and faster response,its ultimate performance is fundamentally limited by the nature of low-frequency AC currents.Herein,highly sensitive skin-like wearable optical sensors are demonstrated by embedding glass micro/nanofibers(MNFs)in thin layers of polydimethylsiloxane(PDMS).Enabled by the transition from guided modes into radiation modes of the waveguiding MNFs upon external stimuli,the skin-like optical sensors show ultrahigh sensitivity(1870 k·Pa^-1),low detection limit(7 mPa)and fast response(10μs)for pressure sensing,significantly exceeding the performance metrics of state-of-the-art electronic skins.Electromagnetic interference(EMI)-free detection of high-frequency vibrations,wrist pulse and human voice are realized.Moreover,a five-sensor optical data glove and a 2×2-MNF tactile sensor are demonstrated.These initial results pave the way toward a new category of optical devices ranging from ultrasensitive wearable sensors to optical skins. | Lei Zhang Jing Pan Zhang Zhang Hao Wu Ni Yao Dawei Cai Yingxin Xu Jin Zhang Guofei Sun Liqiang Wang Weidong Geng Wenguang Jin Wei Fang Dawei Di Limin Tong | 2020 | Opto-Electronic Advances2020，3，3： | 12 |
| 5 | Simultaneous Detection and Removal of Formaldehyde at Room Temperature: Janus Au@ZnO@ZIF-8 Nanoparticles显示文摘The detection and removal of volatile organic compounds(VOCs) are of great importance to reduce the risk of indoor air quality concerns. This study reports the rational synthesis of a dual-functional Janus nanostructure and its feasibility for simultaneous detection and removal of VOCs.The Janus nanostructure was synthesized via an anisotropic growth method, composed of plasmonic nanoparticles,semiconductors, and metal organic frameworks(e.g.,Au@ZnO@ZIF-8). It exhibits excellent selective detection to formaldehyde(HCHO, as a representative VOC) at room temperature over a wide range of concentrations(from 0.25 to100 ppm), even in the presence of water and toluene molecules as interferences. In addition, HCHO was also found to be partially oxidized into non-toxic formic acid simultaneously with detection. The mechanism underlying this technology was unraveled by both experimental measurements and theoretical calculations: ZnO maintains the conductivity, while ZIF-8 improves the selective gas adsorption; the plasmonic effect of Au nanorods enhances the visible-light-driven photocatalysis of ZnO at room temperature. | Dawei Wang Zhiwei Li Jian Zhou Hong Fang Xiang He Puru Jena Jing-Bin Zeng Wei-Ning Wang | 2018 | Nano-Micro Letters2018，10，1： | 6 |
| 6 | Crystal structure of SARS-CoV-2 main protease in complex with protease inhibitor PF-07321332显示文摘Dear Editor,Since December 2019,the pandemic of coronavirus disease 2019(COVID-19)has taken a heavy toll on global health,creating an urgent need to develop effective strategies for prevention and treatment.The etiological agent,known as severe acute respiratory syndrome coronavirus 2(SARSCoV-2),has infected nearly 229.2 million people worldwide with more than 4.7 million deaths as of September 15,2021.Older age and preexisting health conditions are associated with worse clinical prognosis including higher mortality rates(Zhou et al.,2020).The global race to combat this pandemic has led to rapid deployment of numerous effective vaccines against SARS-CoV-2(Tregoning et al.,2021).However,the emergence of viral variants,including the Delta variant(B.1.617.2),compromised vaccine effectiveness with resurgence of SARS-CoV-2 infection among highly vaccinated population(Keehner et al.,2021).Therefore,development of therapeutics against the more conserved viral targets would be essential to contain the spread of COVID-19 and reduce mortality. | Yao Zhao Chao Fang Qi Zhang Ruxue Zhang Xiangbo Zhao Yinkai Duan Haofeng Wang Yan Zhu Lu Feng Jinyi Zhao Maolin Shao Xiuna Yang Leike Zhang Chao Peng Kailin Yang Dawei Ma Zihe Rao Haitao Yang | 2022 | Protein & Cell2022，13，9： | 6 |
| 7 | Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways显示文摘Optimizing the supply of biosynthetic precursors and cofactors is usually an effective metabolic strategy to improve the production of target compounds.Here,the combination of optimizing precursor synthesis and balancing cofactor metabolism was adopted to improve tryptophan production in Escherichia coli.First,glutamine synthesis was improved by expressing heterologous glutamine synthetase from Bacillus subtilis and Bacillus megaterium in the engineered Escherichia coli strain KW001,resulting in the best candidate strain TS-1.Then icd and gdhA were overexpressed in TS-1,which led to the accumulation of 1.060 g/L tryptophan.Subsequently,one more copy of prs was introduced on the chromosome to increase the flux of 5-phospho-α-D-ribose 1-diphosphate followed by the expression of mutated serA and thrA to increase the precursor supply of serine,resulting in the accumulation of 1.380 g/L tryptophan.Finally,to maintain cofactor balance,sthA and pntAB,encoding transhydrogenase,were overexpressed.With sufficient amounts of precursors and balanced cofactors,the engineered strain could produce 1.710 g/L tryptophan after 48 h of shake-flask fermentation,which was 2.76-times higher than the titer of the parent strain.Taken together,our results demonstrate that the combination of optimizing precursor supply and regulating cofactor metabolism is an effective approach for high-level production of tryptophan.Similar strategies could be applied to the production of other amino acids or related derivatives. | Zhu Li Dongqin Ding Huiying Wang Linxia Liu Huan Fang Tao Chen Dawei Zhang | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 5 |
| 8 | HDL impairs osteoclastogenesis and induces osteoclast apoptosis via upregulation of ABCG1 expression显示文摘 | Xinyun Huang Yuan Lv Panpan He Zongbao Wang Fang Xiong Linhao He Xilong Zheng Dawei Zhang Qi Cao Chaoke Tang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 3 |
| 9 | Co_(78)Si_(8)B_(14)metallic glass:A highly efficient and ultra-sustainable Fenton-like catalyst in degrading wastewater under universal pH conditions显示文摘Overcoming the pH limitation and increasing the catalyst reusability remain pressing demands for metal-lic glass(MG)in wastewater remediation.Herein,Co_(78)Si_(8)B_(14)MG ribbons are used to degrade dye wastew-ater by activating hydrogen peroxide(H_(2)O_(2))as Fenton-like catalysts.The Co-based MG catalysts exhibit high degradation efficiency under both acidic and alkaline conditions,and the kinetic reaction rate at pH 10(0.176 min^(−1)) and pH 4(0.089 min^(−1)) is 5.9 and 1.2 times higher than that of the extensively studied Fe-based MG catalysts,respectively.Impressively,the Co-based MG catalysts can be reused up to 20-60 times at universal pH conditions,demonstrating fairly good reusability.The newly discovered Co-based MG catalysts do not follow the classical Fenton reactions with H_(2)O^(2) the way Fe-based MGs do.In an acid environment,hydroxyl radicals play a dominant role in the degradation,while in an alkaline environ-ment,the effect of hydroxyl radicals is weakened,and Co^(3+) ions exert a relatively major function on the degradation.The excellent performance in catalytic activity and reusability at universal pH conditions of the Co-based MGs will inspire the development of MGs in wastewater treatment. | Xindong Qin Jiliang Xu Zhengwang Zhu Zhengkun Li Dawei Fang Huameng Fu Shiming Zhang Haifeng Zhang | 2022 | Journal of Materials Science & Technology2022，，18： | 2 |
| 10 | Density estimated physicochemical properties of alanine-based ionic liquid [C_7mim][Ala] and its application in selective transesterification of soybean oil显示文摘Amino acid ionic liquid (AAIL) based on alanine, 1-n-heptyl-3-methylimidazolium alanine ([C7mim][Ala]), has been prepared and characterized. Since the IL can form strong hydrogen bonds with water, trace amount of water is a problematic impurity in the IL. Using the standard addition method (SAM), the densities were measured in the temperature range from 293.15 to 343.15±0.05 K. On the basis of the experimental data of density, the surface tension , molar volume, and the molecular volume Vm, molar enthalpy of vaporization lgHm0, and the thermal expansion coefficients , for [C7mim][Ala] were estimated using semi-empirical methods. Also the interstice model has been verified with good accordance. Then the [C7mim][Ala] was taken as catalyst in the transesterification reaction with fatty acid, where soybean oil was used as raw material and methanol as reactant. The amount of catalyst, alcohol consumption, reaction time, temperature and other factors of the reaction were investigated. Using GC determination, the conversions were calculated by the internal standard method. The main ingredients of the products were identified by GC-MS as palm methyl ester and linoleic acid methyl ester only, which showed high selectivity in palmitic acid and linoleic acid. | FANG DaWei LI Meng GE RiLe ZANG ShuLiang YANG JiaZhen GAO YanAn | 2012 | Science China Chemistry2012，55，8： | 2 |
| 11 | High-pressing preparation of rock glasses and their elastic properties显示文摘Melt glasses for seven types of rock ranging from acid to basic were prepared under 1.0 GPa on a multi-anvil pressure apparatus, YJ-3000 ton press. Densities and elastic properties of the melt glasses were compared with those described in previous studies. It was found that the glasses melted under 1.0 GPa were consistent in density with both naturally-occurring glassy rocks and artificially prepared glasses melted at ambient pressure. The densities of glasses are negatively correlated with the SiO2 contents and positively correlated with the (MgO+FeO) contents. The compressive velocity (Vp) of glass tends to increase with decreasing SiO2 contents and increasing (MgO+FeO) contents. The shear velocity (Vs) of glass tends to increase slightly with increasing SiO2 contents, which has little connection with the (MgO+FeO) contents. It was calculated from densities and velocities that the elastic moduli of glasses are negatively correlated with the SiO2 contents and positively correlated with the (MgO+FeO) contents. | JIANG Xi ZHOU Wenge XIE Hongsen LIU Yonggang FAN Dawei WAN Fang | 2008 | Chinese Journal Of Geochemistry2008，27，2： | 2 |
| 12 | Generation of strong magnetic fields with a laser-driven coil显示文摘As a promising new way to generate a controllable strong magnetic field, laser-driven magnetic coils have attracted interest in many research fields. In 2013, a kilotesla level magnetic field was achieved at the Gekko XⅡ laser facility with a capacitor–coil target. A similar approach has been adopted in a number of laboratories, with a variety of targets of different shapes. The peak strength of the magnetic field varies from a few tesla to kilotesla, with different spatiotemporal ranges. The differences are determined by the target geometry and the parameters of the incident laser. Here we present a review of the results of recent experimental studies of laser-driven magnetic field generation, as well as a discussion of the diagnostic techniques required for such rapidly changing magnetic fields. As an extension of the magnetic field generation, some applications are discussed. | Zhe Zhang Baojun Zhu Yutong Li Weiman Jiang Dawei Yuan Huigang Wei Guiyun Liang Feilu Wang Gang Zhao Jiayong Zhong Bo Han Neng Hua Baoqiang Zhu Jianqiang Zhu Chen Wang Zhiheng Fang Jie Zhang | 2018 | High Power Laser Science and Engineering2018，6，3： | 2 |
| 13 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | 2013 (9881)2013，，9881： | 2 |
| 14 | Physiological,Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress显示文摘Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity. | Xiaoqin Zhang Tao Tong Bin Tian Yunxia Fang Jiangjie Pan Junjun Zheng Dawei Xue | 2019 | Phyton-International Journal of Experimental Botany2019，88，3： | 2 |
| 15 | Expanding application of CRISPR-Cas9 system in microorganisms显示文摘The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering,synthetic biology,and systems biology.The CRISPR-Cas9 and its derivative tools are one of the best choices for precise genome editing,multiplexed genome editing,and reversible gene expression control in microorganisms.However,challenges remain for applying CRISPR-Cas9 in novel microorganisms,especially those industrial microorganism hosts that are intractable using traditional genetic manipulation tools.How to further extend CRISPR-Cas9 to these microorganisms is being an urgent matter.In this review,we first introduce the mechanism and application of CRISPR-Cas9,then discuss how to optimize CRISPR-Cas9 as genome editing tools,including but not limited to how to reduce off-target effects and Cas9 related toxicity,and how to increase on-target efficiency by optimizing crRNA and sgRNA design. | Jing Zhao Huan Fang Dawei Zhang | 2020 | Synthetic and Systems Biotechnology2020，5，4： | 2 |
| 16 | Effects of solution properties and electric field on the electrospinning of hyaluronic acid显示文摘 | Liu Yang Ma Guiping Fang Dawei | | 0，，02： | 1 |
| 17 | Charge density wave and weak Kondo effect in a Dirac semimetal CeSbTe显示文摘Using angle-resolved photoemission spectroscopy(ARPES) and low-energy electron diffraction(LEED), together with densityfunctional theory(DFT) calculation, we report the formation of charge density wave(CDW) and its interplay with the Kondo effect and topological states in CeSbTe. The observed Fermi surface(FS) exhibits parallel segments that can be well connected by the observed CDWordering vector, indicating that the CDWorder is driven by the electron-phonon coupling(EPC) as a result of the nested FS. The CDW gap is large(~0.3 eV) and momentum-dependent, which naturally explains the robust CDWorder up to high temperatures. The gap opening leads to a reduced density of states(DOS) near the Fermi level(EF), which correspondingly suppresses the many-body Kondo effect, leading to very localized 4 f electrons at 20 K and above. The topological Dirac cone at the X point is found to remain gapless inside the CDW phase. Our results provide evidence for the competition between CDWand the Kondo effect in a Kondo lattice system. The robust CDWorder in CeSbTe and related compounds provide an opportunity to search for the long-sought-after axionic insulator. | Peng Li BaiJiang Lv Yuan Fang Wei Guo ZhongZheng Wu Yi Wu DaWei Shen YueFeng Nie Luca Petaccia Chao Cao Zhu-An Xu Yang Liu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，3： | 1 |
| 18 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | The Lancet2013，，9881： | 1 |
| 19 | Phthalates biodegradation in the environment 显示文摘 | LIANG Dawei ZHANG Tong FANG H H P | 2008 | Appl Microbiol Blot2008，80，2： | 1 |
| 20 | Electrospun sodium alginate/poly(ethylene oxide) core–shell nanofibers scaffolds potential for tissue engineering applications显示文摘 | Guiping Ma Dawei Fang Yang Liu Xiaodan Zhu Jun Nie | 2011 | Carbohydrate Polymers2011，，1： | 1 |
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