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| 1 | Parthenolide protects human lens epithelial cells from oxidative stress-induced apoptosis via inhibition of activation of caspase-3 and caspase-9显示文摘透镜的 apoptosis 上皮的房间作为奔流形成的普通基础被建议了,与是的氧化应力主要原因。这研究被执行对人的透镜的导致 oxidativestress 的 apoptosis 调查草药的成分 parthenolide 的保护的效果上皮(HLE ) 房间和可能的分子的机制包含了。HLE 房间(SRA01-04 ) 在 parthenolide (10, 20 和 50 microM ) 的不同剂量的缺席或存在与 50 microM H (2 ) O (2 ) 被孵化。到学习 apoptosis,房间被词法检查和 Annexin V-propidium 碘化物估计两倍染色的流动 cytometry;调查内在的分子的机制, caspase-3 和 caspase-9 的表示旁边是 assayed 西方的污点和量的 RT-PCR,和 caspase-3 和 caspase-9 的活动我们由 Chemicon caspase 的 remeasured 比色的活动试金工具包。为 18 h 与 H (2 ) O (2 ) 刺激了,, HLE 房间的高部分面对不同集中的 parthenolide 经历了 apoptosis, HLE 房间 apoptosis 堵住的剂量依赖者被观察。H (2 ) O (2 ) 在 HLE 房间导致的表示 ofcaspase-3 和 caspase-9 被 parthenolideboth 显著地在蛋白质和信使 rna 层次减少,并且 caspase-3 和 caspase-9 的激活被 parthenolide 也以一种剂量依赖者方式压制。在结论, parthenolide 通过 caspase-3 andcaspase-9 的激活的抑制阻止 HLEcells 氧化导致压力的 apoptosis,建议对奔流形成的潜在的保护的效果。 | Hangping Yao Xiajing Tang Xueting Shao Lei Feng Nanping Wu Ke Yao | 2007 | Cell Research2007，17，6： | 16 |
| 2 | Triptolide Inhibits Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression in Human Colon Cancer and Leukemia Cells显示文摘Triptolide ( TP ),繁体中文药,被报导了在在几人的肿瘤 celllines.This 研究的肿瘤的活动调查的自体免疫的疾病和施加的反的治疗有效在人的结肠癌房间( SW114 )的 TP 的反肿瘤效果 andmyelocytic 白血病( K562 ),并且阐明可能的分子的机制 involved.SW114 和 K562cells 与 TP ( 0,5,10,20 ,或 50 ng/ml )的不同剂量被对待。房间生存能力被 3- 估计[4,5-dimethylthiazol-2-yl ]-2,5-diphenyltetrazolium 溴化物(MTT ) 。结果证明 TP 以一种剂量依赖者方式禁止了两根肿瘤房间线的增长。为了推进,调查它的机制,产品前列腺素 E2 (PGE2 ) 和氮的氧化物(没有) 被连接酶的免疫吸着剂试金(ELISA ) 测量。我们的数据证明 TP 强烈禁止了生产没有并且有这些的 PGE2.Consistent 结果,表示可诱导合酶(i NOS ) 和 cyclooxygenase-2 (COX-2 ) 都不在 mRNA 水平和蛋白质表示水平两个都是起来调整的由即时 RT-PCR 并且西方的弄污出现。这些结果显示煽动性的因素 COX-2 和 i NOS 活动的抑制能涉及 TP 的 antitumormechanisms。 | Xiangmin TONG~# Shui’er ZHENG~# Jie JIN Lifen ZHU Yinjun LOU and Hangping YAO Institute of Hematologic Disease,Department of Hematology,First Affiliated Hospital,Medical School,Zhejiang University,Hangzhou 310003,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 12 |
| 3 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 4 | Co-expression of IL-18 binding protein and IL-4 regulates Th1/Th2 cytokine response in murine collagen-induced arthritis显示文摘我们构造了包含鼠科的白介素( IL )的 recombinant adenoviral 向量 -18 绑定蛋白质( mIL-18BP )和鼠科的 IL-4 ( mIL-4 )熔化基因( AdmIL-18BP/mIL-4 )并且使用一条基因治疗途径在 T-helper1 和 T-helper2 ( Th1/Th2 )在鼠标平衡的 modulating 调查 IL-18BP 和 IL-4 的角色与导致骨胶原的关节炎(中央情报局)。有中央情报局的老鼠 intra-articularly 与 10 被注射(7 ) AdmIL-18BP/mIL-4,或控制侵入人体气管粘膜的病菌的 pfu/6 亩 l,或与控制车辆(缓冲磷酸盐盐) 。在有 AdmIL-18BP/mIL-4 的 intra 关节的基因治疗以后,肿瘤坏死 factor-alpha (TNF-alpha ) 的浆液层次, gamma 干扰素(IFN-gamma ) , IL-4, IL-10,和在有中央情报局的老鼠的 IL-18 被 ELISA IFN-gamma-expressing 和 IL-4-expressing CD4 (+) T 估计从老鼠 splenocytes 的房间被流动 cytometry 监视。有在 1 , 2 ,和 4 显著地在 AdmIL-18BP/mIL-4 的 intraarticular 注射以后显示出的星期的中央情报局的老鼠增加了 IL-4 和 IL-10 的浆液集中( 0.01 根本预定的 P <
指)但是 IFN-gamma 的极大地减少的浆液集中, TNF-alpha ,并且 IL-18 ( 0.01 根本预定的 P <
指)与控制侵入人体气管粘膜的病菌和缓冲磷酸盐的盐的控制组相比。IFN-gamma-producing CD4 (+) T 房间的百分比显著地响应本地 AdmIL-18BP/mIL-4 处理被减少。房间在 AdmIL-18BP/mIL-4 的本地注射以后在 1 个星期显著地增加了的 IL-4-producing CD4 (+) T 的百分比然后在星期 4 回到了正常。这些数据在本地人在 IL-18BP 和 IL-4 的表示上生产的鼠科的中央情报局在 Th1/Th2 不平衡上显示了重要修改效果。有 IL-18BP 和 IL-4 的联合处理是为风湿性关节炎的有希望的潜在的治疗。 | Jianhang Leng Hangping Yao Junya Shen Keyi Wang Guangchao Zhuo Ziwei Wang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，2： | 5 |
| 5 | Honokiol: an effective inhibitor of tumor necrosis factor-α-induced up-regulation of inflammatory cytokine and chemokine production in human synovial fibroblasts显示文摘在这研究,我们调查了在肿瘤坏死因素(TNF ) 位于 honokiol 的反煽动性的效果下面的机制 -- 刺激风湿性关节炎 synovial 成纤维细胞(RASF ) 。RASF 与 honokiol (020 M ) 预先对待与 TNF-(20 ng/ml ) 被刺激。前列腺素 E2 ( PGE2 )的层次,氮的氧化物(没有),可溶的细胞间的粘附 molecule-1 ( sICAM-1 ),转变生长 factor-1 ( TGF-1 ),单核白血球趋化性的 protein-1 ( MCP-1 ),并且巨噬细胞在上层清液的煽动性的 protein-1 ( MIP-1 )被连接酶的 immunosorbent 试金( ELISA )和 Griess 试金决定。另外, cyclooxygenase-2 (COX-2 ) 的蛋白质表示层次,可诱导的氮的氧化物 synthase (iNOS ) ,和 phosphorylated Akt,原子因素 kappa B (NFB ) ,和细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) 1/2 被西方的污点决定。NFBp65 的表示被 immunocytochemical 分析估计。TNF- 处理显著地起来调整 PGE2 的层次,不在 RASF 的上层清液的 sICAM-1, TGF-1, MCP-1,和 MIP-1,在 RASF 增加了 Akt, IB- , NFB,和 ERK1/2 的 COX-2, iNOS,和导致的 phosphorylation 的蛋白质表示。TNF -- 这些分子的导致的表示被预告的处理与 honokiol 以一种剂量依赖者方式禁止。在 NFBp65 活动的 honokiol 的禁止的效果被 immunocytochemical 分析也证实。在结论, honokiol 是 TNF 的一个潜在的禁止者 -- 在 RASF 导致了煽动性的因素的表示,抓住作为潜在的反煽动性的药答应它。 | Jie Li Xueting Shao Lijuan Wu Tingting Feng Changzhong Jin Meixin Fang Nanping Wu Hangping Yao | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 4 |
| 6 | From SARS to MERS: evidence and speculation显示文摘 | Hainv Gao Hangping Yao Shigui Yang Lanjuan Li | 2016 | Frontiers of Medicine2016，10，4： | 3 |
| 7 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | 2013 (9881)2013，，9881： | 2 |
| 8 | Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding显示文摘Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation as well as plant breeding. In this study, the red fluorescent protein identified in Discosoma sp. coral(DsRed2) was successfully used as a visual marker for cotton genetic engineering. DsRed2 was successfully expressed in two cotton cultivars,JIN668 and YZ1, driven by the Ca MV-35 S promoter via the Agrobacterium-mediated transformation. Our results suggest that DsRed2 expression provides an early-stage selection tool for the transgenic calli via visual observation. Red fluorescence can be detected not only in callus and somatic embryos but also in most tissues and organs of mature plants. The transgenic line Yz-2-DsRed2 was crossed with four different cotton cultivars to assess the transgene heritability and stability in different genetic backgrounds.The heritability of the red color was highly stable when Yz-2-DsRed2 was used as a male parent. The DsRed2 gene expressed 100% in the F_1 hybrids. To investigate the relationship between DsRed2 transcription and DNA methylation, a methylation-specific PCR approach was applied to the Ca MV-35 S promoter region. The results showed a negative association between DNA methylation level in the promoter region and the transgene transcription.Taken together, these findings suggest DsRed2 a visual reporter gene for cotton genetic transformation and molecular breeding programs. | Lin Sun Muna Alariqi Yi Zhu Jianying Li Zelin Li Qing Wang Yajun Li Hangping Rui Xianlong Zhang Shuangxia Jin | 2018 | The Crop Journal2018，6，4： | 2 |
| 9 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | The Lancet2013，，9881： | 1 |
| 10 | B7-H4 enhances oncogenicity and inhibits apoptosis in pancreatic cancer cells显示文摘 | Yun Qian Bo Hong Ling Shen Zhigang Wu Hangping Yao Lihuang Zhang | 2013 | Cell and Tissue Research2013，，： | 1 |
| 11 | Study of levels of IL-18 and IFN -γ in serum of patients with condyloma aeuminatum显示文摘 | Liu Guoying Yao Hangping | 2003 | Journal of Zhejiang University (Medicine Sciences)2003，32，4： | 1 |
| 12 | Compiling l,adder Diagram into Instruction List to comply with IEC 61131-3显示文摘 | Yan Yi Hangping Zhang | 2010 | Computers in Industry2010，61，5： | 1 |
| 13 | B7-H4 enhances oncogenicity and inhibits apoptosis in pancreatic cancer cells显示文摘 | Yun Qian Bo Hong Ling Shen Zhigang Wu Hangping Yao Lihuang Zhang | 2013 | Cell and Tissue Research2013，，1： | 1 |
| 14 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | The Lancet2013，，9881： | 1 |
| 15 | Mechanism of apoptotic induction on T24 cells by honokiol and its synergistic anticancer effect in combination with hydroxycamptothecin显示文摘OBJECTIVE:To investigate the mechanism of honokiol(HNK)on bladder cancer cells and its synergistic anticancer effect with hydroxycamptothecin(HCPT).METHODS:Control,HNK,HCPT,and HNK plus HCPT groups were established.The morphological characteristics of T24 cells were examined microscopically.The maximal experimental concentration of HNK and HCPT were determined according to IC10 detected by MTT.T24 cell viability and the percentage of apoptotic cells were assessed on the basis of MTT and flow cytometric analysis.The expression of caspase-3,caspase-9,phosphorylated nuclear factor-kappa B(NF-κB)-p65,Akt,and extracellular signal-regulated kinase(ERK)proteins were analyzed by Western blot.RESULTS:Apoptosis in T24 cells was observed microscopically in both the HNK and HCPT groups and even more obvious in the HNK plus HCPT groups.The percentage of T24 cell viability decreased down to 19.41%,and the percentage of apoptotic cells rose to 54.08% when treated with HNK plus HCPT in an HNK dose-dependent manner.The induction of caspase-3 and caspase-9 proteins and the inhibition of phosphorylation of NF-κB-p65,Akt,and ERK proteins in T24 cells were demonstrated in the HNK groups,and more significantly in the HNK plus HCPT groups,but not in the HCPT group.CONCLUSION:The anticancer effect of HNK may be due to the activation of the caspase pathway and inhibition of phosphorylation of NF-κB,Akt,and ERK.HNK in combination with HCPT produces a synergistic cell-killing effect on bladder cancer cells. | LOU Guoguang LUO Yiting XIE Liping YAO Hangping HU Qing | 2021 | Journal of Traditional Chinese Medicine2021，41，4： | 1 |
| 16 | Extraction and purification of squalene from amaranthus grain 显示文摘 | HE Hangping CAI Yinzhong SUN Mei | 2002 | J Agric Food Chem2002，50，2： | 1 |
| 17 | Unusual global outbreak of monkeypox:what should we do?显示文摘Recently,monkeypox has become a global concern amid the ongoing COVID-19 pandemic.Monkeypox is an acute rash zoonosis caused by the monkeypox virus,which was previously concentrated in Africa.The re-emergence of this pathogen seems unusual on account of outbreaks in multiple nonendemic countries and the incline to spread from person to person.We need to revisit this virus to prevent the epidemic from getting worse.In this review,we comprehensively summarize studies on monkeypox,including its epidemiology,biological characteristics,pathogenesis,and clinical characteristics,as well as therapeutics and vaccines,highlighting its unusual outbreak attributed to the transformation of transmission.We also analyze the present situation and put forward countermeasures from both clinical and scientific research to address it. | Miaojin Zhu Jia Ji Danrong Shi Xiangyun Lu Baohong Wang Nanping Wu Jie Wu Hangping Yao Lanjuan Li | 2022 | Frontiers of Medicine2022，16，4： | 1 |
| 18 | Computation in the Qiantang estuary with consideration of sediment transport in tidal flat显示文摘 | Han Zengcui Chen Hangping | 1987 | China Ocean Engineering1987，，2： | 1 |
| 19 | Photoluminescence of Sr2CeO4 Phosphors Prepared by Microwave Calcination and Pulsed Laser Deposition显示文摘 | Tang Yongxin Guo Hangping Qin Qizong | 2002 | Solid State Communications2002，121，： | 1 |
| 20 | Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome显示文摘 | Yu Chen Weifeng Liang Shigui Yang Nanping Wu Hainv Gao Jifang Sheng Hangping Yao Jianer Wo Qiang Fang Dawei Cui Yongcheng Li Xing Yao Yuntao Zhang Haibo Wu Shufa Zheng Hongyan Diao Shichang Xia Yanjun Zhang Kwok-Hung Chan Hoi-Wah Tsoi Jade Lee-Lee Teng We | 2013 | The Lancet2013，，9881： | 1 |
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