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| 1 | Oxygen Requirements for the Cambrian Explosion显示文摘Hypoxic tolerance experiments may be helpful to constrain the oxygen requirement for animal evolution. Based on literature review, available data demonstrate that fishes are more sensitive to hypoxia than crustaceans and echinoderms, which in turn are more sensitive than annelids, whilst mollusks are the least sensitive. Mortalities occur where O_2 concentrations are below 2.0 mg/L, equivalent to saturation with oxygen content about 25% PAL(present atmospheric level). Therefore, the minimal oxygen requirement for maintaining animal diversity since Cambrian is determined as 25% PAL. The traditional view is that a rise in atmospheric oxygen concentrations led to the oxygenation of the ocean, thus triggering the evolution of animals. Geological and geochemical studies suggest a constant increase of the oxygen level and a contraction of anoxic oceans during Ediacaran–Cambrian transition when the world oceans experienced a rapid diversification of metazoan lineages. However, fossil first appearances of animal phyla are obviously asynchronous and episodic, showing a sequence as: basal metazoans>lophotrochozoans>ecdysozoans and deuterostomes. According to hitherto known data of fossil record and hypoxic sensitivity of animals, the appearance sequence of different animals is broadly consistent with their hypoxic sensitivity: animals like molluscs and annelids that are less sensitive to hypoxia appeared earlier, while animals like echinoderms and fishes that are more sensitive to hypoxia came later. Therefore, it is very likely that the appearance order of animals is corresponding to the increasing oxygen level and/or the contraction of anoxic oceans during Ediacaran–Cambrian transition. | Xingliang Zhang Linhao Cui | 2016 | Journal of Earth Science2016，27，2： | 6 |
| 2 | Seasonal Cumulative Effect of Ural Blocking Episodes on the Frequent Cold events in China during the Early Winter of 2020/21显示文摘Starting in mid-November,China was hit by several cold events during the early winter of 2020/21.The lowest temperature observed at Beijing station on 7 January reached−19.6°C.In this paper,we show that the outbreak of the record-breaking extreme cold event can be attributed to a huge merging Ural blocking(UB)ridge over the Eurasian region.The sea-ice cover in the Kara and East Siberia Seas(KESS)in autumn was at its lowest value since 1979,which could have served as a precursor signal.Further analysis shows that several successive UB episodes occurred from 1 September 2020 to 10 January 2021.The persistent UB that occurred in late September/early October 2020 may have made an important contribution to the October historical minimum of sea ice in the KESS region.Our results also show that,after each UB episode in winter,significant upward propagation of wave activity occurred around 60°E,which resulted in weakening the stratospheric vortex.Meanwhile,each UB episode also caused a significant reduction in sea-ice extent in KESS and a significant weakening of the westerly jet in mid-high-latitude Eurasia.Results suggest that the Arctic vortex,which is supposed to enhance seasonally,became weaker and more unstable than the climatic mean under the seasonal cumulative effects of UB episodes,KESS warming,and long-lasting negative-phase North Atlantic Oscillation(NAO-).Those seasonal cumulative effects,combined with the impact of La Niña winter,led to the frequent occurrence of extreme cold events. | Yao YAO Wenqi ZHANG Dehai LUO Linhao ZHONG Lin PEI | 2022 | Advances in Atmospheric Sciences2022，39，4： | 4 |
| 3 | HDL impairs osteoclastogenesis and induces osteoclast apoptosis via upregulation of ABCG1 expression显示文摘 | Xinyun Huang Yuan Lv Panpan He Zongbao Wang Fang Xiong Linhao He Xilong Zheng Dawei Zhang Qi Cao Chaoke Tang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 3 |
| 4 | SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants显示文摘RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants. | Linhao Ge Buwei Cao Rui Qiao Hongguang Cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li | 2023 | Molecular Plant2023，16，3： | 2 |
| 5 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 6 | Facile synthesis and self-assembly of hierarchical porous Ni O nano/micro spherical superstructures for high performance supercapacitors显示文摘 | YUAN Changzhou ZHANG Xiaogang SUN Linhao | 2009 | Journal of Materials Chemistry2009，19，32： | 1 |
| 7 | Simultaneous ultraviolet,visible,and near-infrared continuous-wave lasing in a rare-earth-doped microcavity显示文摘Microlaser with multiple lasing bands is critical in various applications,such as full-color display,optical communications,and computing.Here,we propose a simple and efficient method for homogeneously doping rare earth elements into a silica whispering-gallery microcavity.By this method,an Er-Yb co-doped silica microsphere cavity with the highest quality(Q)factor(exceeding 108)among the rare-earth-doped microcavities is fabricated to demonstrate simultaneous and stable lasing covering ultraviolet,visible,and near-infrared bands under room temperature and a continuous-wave pump.The thresholds of all the lasing bands are estimated to be at the submilliwatt level,where both the ultraviolet and violet continuous wave upconversion lasing from rare earth elements has not been separately demonstrated under room temperature until this work.This ultrahigh-Q doped microcavity is an excellent platform for highperformance multiband microlasers,ultrahigh-precision sensors,optical memories,and cavity-enhanced light–matter interaction studies. | Bo Jiang Song Zhu Linhao Ren Lei Shi Xinliang Zhang | 2022 | Advanced Photonics2022，4，4： | 1 |
| 8 | Higher-order principal component pursuit via tensor approximation and convex optimization显示文摘Recovering the low-rank structure of data matrix from sparse errors arises in the principal component pursuit(PCP). This paper exploits the higher-order generalization of matrix recovery,named higher-order principal component pursuit(HOPCP), since it is critical in multi-way data analysis. Unlike the convexification(nuclear norm) for matrix rank function, the tensorial nuclear norm is still an open problem. While existing preliminary works on the tensor completion field provide a viable way to indicate the low complexity estimate of tensor, therefore, the paper focuses on the low multi-linear rank tensor and adopt its convex relaxation to formulate the convex optimization model of HOPCP. The paper further propose two algorithms for HOPCP based on alternative minimization scheme: the augmented Lagrangian alternating direction method(ALADM) and its truncated higher-order singular value decomposition(ALADM-THOSVD) version. The former can obtain a high accuracy solution while the latter is more efficient to handle the computationally intractable problems. Experimental results on both synthetic data and real magnetic resonance imaging data show the applicability of our algorithms in high-dimensional tensor data processing. | Sijia Cai Ping Wang Linhao Li Chuhan Zhang | 2014 | Journal of Systems Engineering and Electronics2014，25，3： | 1 |
| 9 | Template-Free Synthesis of Ordered Mesoporous NiO/Poly (Sodium-4-Styrene Sulfonate) Functionalized Carbon Nanotubes Composite for Electrochemical Capacitors显示文摘We report the first example of a practical and efficient template-free strategy for synthesizing ordered mesoporous NiO/poly(sodium-4-styrene sulfonate)(PSS)functionalized carbon nanotubes(FCNTs)composites by calcining a Ni(OH)_(2)/FCNTs precursor prepared by refl uxing an alkaline solution of Ni(NH_(3))x^(2)+and FCNTs at 97 oC for 1 h.The morphology and structure were characterized by X-ray diffraction,scanning electron microscopy,and transmission electron microscopy.Thermal decomposition of the precursor results in the formation of ordered mesoporous NiO/FCNTs composite(ca.48 wt%NiO)with large specifi c surface area.Due to its enhanced electronic conductivity and hierarchical(meso-and macro-)porosity,composite simultaneously meets the three requirements for energy storage in electrochemical capacitors at high rate,namely,good electron conductivity,highly accessibleelectrochemical surface areas owing to the existence of mesopores,and efficient mass transport from the macropores.Electrochemical data demonstrated that the ordered mesoporous NiO/FCNTs composite is capable of delivering a specifi c capacitance(SC)of 526 F/g at 1 A/g and a SC of 439 F/g even at 6 A/g,and show a degradation of only ca.6%in SC after 2000 continuous charge/discharge cycles. | Changzhou Yuan Shenglin Xiong Xiaogang Zhang Laifa Shen Fang Zhang Bao Gao Linhao Su | 2009 | Nano Research2009，2，9： | 1 |
| 10 | Arbuscular mycorrhizal fungi communities associated with wild plants in a coastal ecosystem显示文摘Arbuscular mycorrhizal fungi(AMF)form a near-ubiquitous mutualistic association with roots to help plants withstand harsh environments,and play a key role in the establishment of coastal beach plant communities.Yet little is known about the structure and composition of AMF communities on coastal beaches of eastern China.In this study,we investigated the occurrence,community composition and diversity of AMF associated with common wild plants on a coastal beach of North Jiangsu,China.Almost all of the local wild species were colonized by AMF except for Chenopodium album L.Thirty-seven AMF species were isolated from the rhizosphere belonging to 12 genera in seven families.Glomus was the dominant genus and Funneliformis mosseae the dominant species.The colonization,spore composition and diversity of AMF were strongly related to edaphic factors.Sodium(Na^(+))ions in the soil significantly and negatively affected the colonization rate by AMF and both soil Na^(+)levels and pH had a significant negative effect on AMF spore density and evenness.However,there was a significant positive correlation between species richness and total organic carbon.The results provide insights into soil factors affecting native AMF communities in coastal beach habitats which could benefit vegetation recovery and soil reclamation efforts. | Jinping Wang Shilin Ma G.Geoff Wang Linhao Xu Zhiyuan Fu Juan Song Jinchi Zhang | 2021 | Journal of Forestry Research2021，32，2： | 1 |
| 11 | Estimating returns to scale of Chinese airport airside activities using the CCR-0-objective RTS method显示文摘 | Zhang Baocheng Wang Jianzhong Meng Linhao | 2013 | Journal of the Operational Research Society2013，65，5： | 1 |
| 12 | Plant and animal positive-sense single-stranded RNA viruses encode small proteins important for viral infection in their negative-sense strand显示文摘Positive-sense single-stranded RNA(+ssRNA)viruses,the most abundant viruses of eukaryotes in nature,require the synthesis of negative-sense RNA(-RNA)using their genomic(positive-sense)RNA(+RNA)as a template for replication.Based on current evidence,viral proteins are translated via viral+RNAs,whereas-RNA is considered to be a viral replication intermediate without coding capacity.Here,we report that plant and animal+ssRNA viruses contain small open reading frames(ORFs)in their-RNA(reverse ORFs[rORFs]).Using turnip mosaic virus(TuMV)as a model for plant+ssRNA viruses,we demonstrate that small proteins encoded by rORFs display specific subcellularlocalizations,and confirm the presence of rORF2 in infected cells through mass spectrometry analysis.The protein encoded by TuMV rORF2 forms punctuate granules that are localized in the perinuclear region and co-localized with viral replication complexes.The rORF2 protein can directly interact with the viral RNA-dependent RNA polymerase,and mutation of rORF2 completely abolishes virus infection,whereas ectopic expression of rORF2 rescues the mutant virus.Furthermore,we show that several rORFs in the-RNA of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have the ability to suppress type l interferon production and facilitate the infection of ve-sicular stomatitis virus.In addition,we provide evidence that TuMV might utilize internal ribosome entry sites to translate these small rORFs.Taken together,these findings indicate that the-RNA of+ssRNA vi-ruses can also have the coding capacity and that small proteins encoded therein play critical roles in viral infection,revealing a viral proteome larger than previously thought. | Pan Gong Qingtang Shen Mingzhen Zhang Rui Qiao Jing Jiang Lili Su Siwen Zhao Shuai Fu Yu Ma Linhao Ge Yaqin Wang Rosa Lozano-Durán Aiming Wang Fangfang Li Xueping Zhou | 2023 | Molecular Plant2023，16，11： | 0 |
| 13 | Detector development at the Back-n white neutron source显示文摘Purpose Back-n is a white neutron beamline at China spallation neutron source,which was established in the year of 2018.It is a powerful facility for nuclear data measurement,neutron detector calibration,and radiation effect research.Method A series of detectors were built for different experiments,including beam monitoring,beam profile measurement,neutron induced secondaries(fission fragments,light charged particles and gamma)cross section measurement,and neutron resonance radiography,etc.A common digitization electronics and a cluster-based DAQ were developed for these detector systems.Most detectors have been employed at Back-n and serviced for experiments from the beginning of the beamline running.Results and conclusion As an overview of detectors of Back-n,the details of the detector design and the experiment performing are described in this paper.Some developing systems,e.g.,MTPC and B-MCP,are also included. | Fan Ruirui Li Qiang Bao Jie Li Yang Liu Rong Jiang Wei Ren Jie Zhang Qiwei Cao Ping Gu Minhao Ren Zhizhou Yi Han Tang Jingyu An Qi Bai Haofan Bai Jiangbo Chen Qiping Chen Yonghao Chen Zhen Cui Zengqi Fan Anchuan Feng Changqing Feng Fanzhen Gao Keqing Han Changcai Han Zijie He Guozhu He Yongcheng Hong Yang Hu Yiwei Huang Hanxiong Jia Weihua Jiang Haoyu Jiang Zhijie Jin Zhengyao Kang Ling Li Bo Li Chao Li Gong Li Jiawen Li Xiao Liu Jie Liu Shubin Luan Guangyuan Ning Changjun Qi Binbin Ruan Xichao Song Zhaohui Sun Kang Tan Zhixin Tang Shengda Wang Pengcheng Wang Zhaohui Wen Zhongwei Wu Xiaoguang Wu Xuan Xie Likun Yang Yiwei Yu Yongji Zhang Guohui Zhang Linhao Zhang Mohan Zhang Xianpeng Zhang Yuliang Zhang Yue Zhang Zhiyong Zhao Maoyuan Zhou Luping Zhou Zhihao Zhu Kejun The CSNS Back-n Collaboration | 2023 | Radiation Detection Technology and Methods2023，7，2： | 0 |
| 14 | χ(^(2)) nonlinear photonics in integrated microresonators显示文摘Second-order(χ^((2))) optical nonlinearity is one of the most common mechanisms for modulating and generating coherent light in photonic devices.Due to strong photon confnement and long photon lifetime,integrated microresonators have emerged as an ideal platform for investigation of nonlinear optical efects.However,existing silicon-based materials lack a χ^((2)) response due to their centrosymmetric structures.A variety of novel material platforms possessing χ^((2)) nonlinearity have been developed over the past two decades.This review comprehensively summarizes the progress of second-order nonlinear optical efects in integrated microresonators.First,the basic principles of χ^((2)) nonlinear efects are introduced.Afterward,we highlight the commonly used χ^((2)) nonlinear optical materials,including their material properties and respective functional devices.We also discuss the prospects and challenges of utilizing χ^((2)) nonlinearity in the feld of integrated microcavity photonics. | Pengfei Liu Hao Wen Linhao Ren Lei Shi Xinliang Zhang | 2023 | Frontiers of Optoelectronics2023，16，3： | 0 |
| 15 | Multi-functionalized nanofibers with reactive oxygen species scavenging capability and fibrocartilage inductivity for tendon-bone integration显示文摘The presence of excessive reactive oxygen species(ROS)after injuries to the enthesis could lead to cellular oxidative damage,high inflammatory response,chronic inflammation,and limited fibrochondral inductivity,making tissue repair and functional recovery difficult.Here,a multifunctional silk fibroin nanofiber modified with polydopamine and kartogenin was designed and fabricated to not only effectively reduce inflammation by scavenging ROS in the early stage of the enthesis healing but also enhance fibrocartilage formation with fibrochondrogenic induction in the later stages.The in vitro results confirmed the antioxidant capability and the fibrochondral inductivity of the functionalized nanofibers.In vivo studies showed that the multifunctional nanofiber can significantly improve the integration of tendon-bone and accelerate the regeneration of interface tissue,resulting in an excellent biomechanical property.Thus,the incorporation of antioxidant and bio-active molecules into extracellular matrix-like biomaterials in interface tissue engineering provides an integrative approach that facilitates damaged tissue regeneration and functional recovery,thereby improving the clinical outcome of the engineered tissue. | Peixing Chen Sixiang Wang Zhi Huang Yan Gao Yu Zhang Chunli Wang Tingting Xia Linhao Li Wanqian Liu Li Yang | 2021 | Journal of Materials Science & Technology2021，，11： | 0 |
| 16 | The Regulatory Effect of Braided Silk Fiber Skeletons with Differential Porosities on In Vivo Vascular Tissue Regeneration and Long-Term Patency显示文摘The development of small-diameter vascular grafts that can meet the long-term patency required for implementation in clinical practice presents a key challenge to the research field.Although techniques such as the braiding of scaffolds can offer a tunable platform for fabricating vascular grafts,the effects of braided silk fiber skeletons on the porosity,remodeling,and patency in vivo have not been thoroughly investigated. | Xili Ding Weirong Zhang Peng Xu Wentao Feng Xiaokai Tang Xianda Yang Lizhen Wang Linhao Li Yan Huang Jing Ji Diansheng Chen Haifeng Liu Yubo Fan | 2023 | Research2023，，2： | 0 |
| 17 | CH02 peptide promotes ex vivo expansion of umbilical cord blood-derived CD34+hematopoietic stem/progenitor cells显示文摘Umbilical cord blood(UCB)is an advantageous source for hematopoietic stem/progenitor cell(HSPC)transplantation,yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable.To overcome these obstacles,we systematically evaluate the feasibility of our newly identified CH02 peptide for ex vivo expansion of CD34^(+)UCB-HSPCs.We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling.Notably,the CH02-based cocktails are adequate for boosting 12-fold ex vivo expansion of UCB-HSPCs.Meanwhile,CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory factors.Together,our data indicate the advantages of the CH02-based strategy for ex vivo expansion of CD34^(+)UCB-HSPCs,which will provide new strategies for further development of large-scale HSPC preparation for clinical purposes. | Yiqi Yang Bihui Zhang Junye Xie Jingsheng Li Jia Liu Rongzhan Liu Linhao Zhang Jinting Zhang Zijian Su Fu Li Leisheng Zhang An Hong Xiaojia Chen | 2023 | Acta Biochimica et Biophysica Sinica2023，55，10： | 0 |
| 18 | DES Mutagenesis of Antagonistic Streptomyces of Botryosphaeria dothidea and Optimization of Its Fermentation Conditions显示文摘[Objective] The paper was to improve the antimicrobial effect of antagonistic Streptomyces on Botryosphaeria dothidea. [Method]Different concentrations of DES were adopted for mutagenic treatment of antagonistic Streptomyces F-58. After fermentation,the mutant strain numbered F-58-06 with genetic stability and good antimicrobial effect was selected among 200 mutant strains; the biological activity of the strain was determined with B. dothidea as the indicator fungus,and the best concentration of DES was 2%. The fermentation conditions of antagonistic substances of Streptomyces strain F-58-06 produced in shake flasks were optimized using the method of single factor and orthogonal experimental design. [Result] The optimal fermentation conditions were carbon source corn flour,nitrogen source KNO_3,initial p H 7,loading volume 30 m L in 250 m L shake flask,inoculation volume 3%,incubation time 132 h. Based on orthogonal experiments,the best cultured medium ratio was corn flour 4%,KNO_30. 5%,Ca CO_30. 2%,Na Cl 0. 35%,Mg SO_4·7H_2O 0. 15%. [Conclusion]After mutation and optimization of culture medium,the antimicrobial activity of Streptomyces F-58 was greatly improved,and the average inhibition zone diameter was 1. 35 times of that of the original strain. | Li Limei Zheng Tiantian Sui Yan Li Xin Sun Jinghua Zhang Linhao Zhu Chunyu Chen Yuequ | 2017 | Plant Diseases and Pests2017，8，1： | 0 |
| 19 | Highly efficient catalytic valorization of biomass-derived hexoses and furfuryl alcohol in the presence of polymer-based catalysts显示文摘The catalytic transformation of furfuryl alcohol and hexose into value-added chemicals have been investigated with a series of polymer-based catalysts including poly(styrenesulfonic acid)(PSS),graphite oxide-doped poly(styrenesulfonic acid)(PSS-GO),and graphite-doped poly(-styrenesulfonic acid)(PSS-C).It is found that the selective conversion of furfuryl alcohol to methyl levulinate(MLE)was successfully performed with PSS as the catalyst,in which a 96.4%yield is attained in methanol solvent.Moreover,the efficient dehydration of D-fructose to produce 5-hydroxymethylfurfural(HMF)has also been achieved using PSS-GO as the catalyst,where a 76.5%yield of HMF was obtained in Nmethylpyrrodinone solvent.The effects of reaction temperature,time and solvent were investigated.Furthermore,the used catalysts have been respectively characterized by XRD,TG,FTIR,SEM and TEM techniques to reveal the physical properties and structures of these polymeric catalytic materials. | Yangyang Zhang Xinli Tong Linhao Yu Lingwu Meng Pengfei Guo Song Xue | 2019 | Green Energy & Environment2019，4，4： | 0 |
| 20 | Geminiviral C2 proteins inhibit active autophagy to facilitate virus infection by impairing the interaction of ATG7 and ATG8显示文摘Autophagy is a conserved intracellular degradation process that plays an active role in plant response to virus infections.Here we report that geminiviruses counteract activated autophagymediated antiviral defense in plant cells through the C2 proteins they encode.We found that,in Nicotiana benthamiana plants,tomato leaf curl Yunnan virus(TLCYnV)infection upregulated the transcription levels of autophagy-related genes(ATGs).Overexpression of NbATG5,NbATG7,or NbATG8a in N.benthamiana plants decreased TLCYnV accumulation and attenuated viral symptoms.Interestingly,transgenic overexpression of NbATG7 promoted the growth of N.benthamiana plants and enhanced plant resistance to TLCYnV.We further revealed that the C2 protein encoded by TLCYnV directly interacted with the ubiquitinactivating domain of ATG7.This interaction competitively disrupted the ATG7–ATG8 binding in N.benthamiana and Solanum lycopersicum plants,thereby inhibiting autophagy activity.Furthermore,we uncovered that the C2-mediated autophagy inhibition mechanism was conserved in three other geminiviruses.In summary,we discovered a novel counter-defensive strategy employed by geminiviruses that enlists their C2 proteins as disrupters of ATG7–ATG8 interactions to defeat antiviral autophagy. | Buwei Cao Linhao Ge Mingzhen Zhang Fangfang Li Xueping Zhou | 2023 | Journal of Integrative Plant Biology2023，65，5： | 0 |
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