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| 1 | Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘Reverse transcription-polymerase chain reaction(RT-PCR)is an essential method for specific diagnosis of SARS-CoV-2 infection.Unfortunately,false negative test results are often reported.In this study,we attempted to determine the principal causes leading to false negative results of RT-PCR detection of SARS-CoV-2 RNAs in respiratory tract specimens.Multiple sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercialfluorescent RT-PCR kit targeting the ORF1 ab and N regions of SARS-CoV-2 genome.The RNA level of a cellular housekeeping gene ribonuclease P/MRP subunit p30(RPP30)in these specimens was also assessed by RT-PCR.Data for a total of 1052 samples were retrospectively re-analyzed and a strong association between positive results in SARS-CoV-2 RNA tests and high level of RPP30 RNA in respiratory tract specimens was revealed.By using the ROC-AUC analysis,we identified Ct cutoff values for RPP30 RT-PCR which predicted false negative results for SARS-CoV-2 RT-PCR with high sensitivity(95.03%–95.26%)and specificity(83.72%–98.55%)for respective combination of specimen type and ampli-fication reaction.Using these Ct cutoff values,false negative results could be reliably identified.Therefore,the presence of cellular materials,likely infected host cells,are essential for correct SARS-CoV-2 RNA detection by RT-PCR in patient specimens.RPP30 could serve as an indicator for cellular content,or a surrogate indicator for specimen quality.In addition,our results demonstrated that false negativity accounted for a vast majority of contradicting results in SARS-CoV-2 RNA test by RT-PCR. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 5 |
| 2 | Japanese Encephalitis Virus NS1' Protein Antagonizes Interferon Beta Production显示文摘Japanese encephalitis virus(JEV) is a mosquito-borne virus and the major cause of viral encephalitis in Asia. NS1', a52-amino acid C-terminal extension of NS1, is generated with a-1 programmed ribosomal frameshift and is only present in members of the Japanese encephalitis serogroup of flaviviruses. Previous studies demonstrated that NS1' plays a vital role in virulence, but the mechanism is unclear. In this study, an NS1' defected(rG66A) virus was generated. We found that rG66A virus was less virulent than its parent virus(pSA14) in wild-type mice. However, similar mortality caused by the two viruses was observed in an IFNAR knockout mouse model. Moreover, we found that rG66A virus induced a greater type Ⅰ interferon(IFN) response than that by pSA14, and JEV NS1' significantly inhibited the production of IFN-b and IFN-stimulated genes. Taken together, our results reveal that NS1' plays a vital role in blocking type I IFN production to help JEV evade antiviral immunity and benefit viral replication. | Dengyuan Zhou Fan Jia Qiuyan Li Luping Zhang Zheng Chen Zikai Zhao Min Cui Yunfeng Song Huanchun Chen Shengbo Cao Jing Ye | 2018 | Virologica Sinica2018，33，6： | 3 |
| 3 | Variation analysis of growth traits of four poplar clones under different water and fertilizer management显示文摘To determine appropriate quantities of water and fertilizer required for early growth of hybrid poplar cuttings,we recorded the growth traits of four clones grown under four factors(irrigation and nitrogen,phosphorus,and potassium fertilizers),each with four levels,using an orthogonal experimental design.A logistic model was used to estimate growth in height.The growth curves for tree height were sigmoid,and the model R^2 values were greater than 0.9,which indicated that the fit was highly significant.ANOVA results for tree height and basal diameter indicated that all sources of variance showed significant differences(p<0.001).The average tree height and basal diameter for all the four clones under the different treatments ranged from 155.39 to 235.04 cm,and from 13.71 to 17.42 mm,respectively.A highly positive correlation between the extreme k value and tree height was observed,suggesting that the k value was an accurate estimation of tree height.For model parameters,the earliest average time point for the onset of the rapid growth period of poplar clones was 131 d,and the highest average increment in tree height during the rapid growth period was 138.78 cm.The highest average tree height for all clones under each factor was 219,210.51,200,and 201 cm when treated with either 1200 mL of water applied every third day,3 g of nitrogen,0 g of phosphorus,or 0 g of potassium,respectively.The most suitable treatment for the early growth of hybrid poplar cuttings,as suggested by the developed logistic model,was 1200 mL of water applied every third day and three applications of 1 g nitrogen(in the form of CH4N2O). | Ammar Khalil Mohamed Ahmed Luping Jiang Fang Wang Song Chen Xueyan Zhou Xiaona Pei Xiyang Zhao Guanzheng Qu | 2020 | Journal of Forestry Research2020，31，1： | 2 |
| 4 | Event-related potential correlates of the collective self-relevant effect显示文摘 | Ke Zhao Jiajin Yuan Yiping Zhong Yunshi Peng Jie Chen Luping Zhou Wei Fan Daoqun Ding | 2009 | Neuroscience Letters2009，，1： | 1 |
| 5 | Improving Latino Disaster Preparedness Using Social Networks显示文摘 | David P. Eisenman Deborah Glik Lupe Gonzalez Richard Maranon Qiong Zhou Chi-Hong Tseng Steven M. Asch | 2009 | American Journal of Preventive Medicine2009，，6： | 1 |
| 6 | Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody显示文摘 | LI Luping ZHOU Lei YU Yang | 2009 | Diag Microbiol Infec Dis2009，63，2： | 1 |
| 7 | Mul- timodal classification of Alzheimer' s disease and mild cog- nitive impairment 显示文摘 | Zhang Daoqiang Wang Yaping Zhou Luping | 2011 | Neuroimage2011，55，3： | 1 |
| 8 | The impact of spatial mismatch on residents in low-income housing neighbourhoods: a study of the Guangzhou metropolis, China 显示文摘 | ZHOU Suhong WU Zhidong CHENG Luping | 2013 | Urban Studies2013，50，9： | 1 |
| 9 | Determination of 23 phthalic acid esters in food by liquid chromatography tandem mass spectrometry显示文摘 | Dunming Xu Xiaojun Deng Enhua Fang Xianghua Zheng Yu Zhou Liyi Lin Luping Chen Ming Wu Zhiqiang Huang | 2014 | Journal of Chromatography A2014，，： | 1 |
| 10 | Tunable metamaterial absorber based on resonant strontium titanate artificial atoms显示文摘Dynamic control of the absorption frequency and intensity of metamaterial absorbers has attracted considerable attention,and many kinds of tunable metamaterial absorbers have been proposed.Unfortunately,due to the integration of separate resonant unit and tunable unit,these designed metamaterial absorbers suffer from complex structure and low sensitivity.We numerically and experimentally demonstrate a tunable metamaterial absorber composed of artificial dielectric atoms as both resonant and tunable unit arrayed periodically in the background matrix on the metallic plate.Polarization insensitive and wide incident angle absorption band with simulated and experimental absorptivity of 99%and 96%at 9.65 GHz are achieved at room temperature.The absorption frequency can be gradually modulated by temperature,however,the absorption intensity at working frequency remains near unity.The dielectric atoms based tunable metamaterial absorbers with simple structure have potential applications as tempe rature sensors and frequency selective thermal emitters. | Xiaoming Liu Zhiyu Ren Tian Yang Luping Chen Qiang Wang Ji Zhou | 2021 | Journal of Materials Science & Technology2021，，3： | 1 |
| 11 | Farnesyl diphosphate synthase is abundantly expressed and regulated by androgen in rat prostatic epithelial cells显示文摘 | Feng Jiang Luping Yang Xiaoyan Cai Jomol Cyriac Ishaiahu Shechter Zhou Wang | 2001 | Journal of Steroid Biochemistry and Molecular Biology2001，，2： | 1 |
| 12 | OsCPL3 is involved in brassinosteroid signaling by regulating OsGSK2 stability显示文摘Glycogen synthase kinase 3(GSK3)proteins play key roles in brassinosteroid(BR)signaling during plant growth and development by phosphorylating various substrates.However,how GSK3 protein stability and activity are themselves modulated is not well understood.Here,we demonstrate in vitro and in vivo that C-TERMINAL DOMAIN PHOSPHATASELIKE 3(Os CPL3),a member of the RNA Pol II CTD phosphatase-like family,physically interacts with Os GSK2 in rice(Oryza sativa).Os CPL3 expression was widely detected in various tissues and organs including roots,leaves and lamina joints,and was induced by exogenous BR treatment.Os CPL3 localized to the nucleus,where it dephosphorylated Os GSK2 at the Ser-222 and Thr-284 residues to modulate its protein turnover and kinase activity,in turn affecting the degradation of BRASSINAZOLERESISTANT 1(BZR1)and BR signaling.Loss of Os CPL3 function resulted in higher Os GSK2 abundance and lower Os BZR1 levels,leading to decreased BR responsiveness and alterations in plant morphology including semi-dwarfism,leaf erectness and grain size,which are of fundamental importance to crop productivity.These results reveal a previously unrecognized role for Os CPL3 and add another layer of complexity to the tightly controlled BR signaling pathway in plants. | Luping Gong Shenghao Liao Wen Duan Yongqiang Liu Dongmei Zhu Xiaosheng Zhou Baoping Xue Chengcai Chu Yun-Kuan Liang | 2022 | Journal of Integrative Plant Biology2022，64，8： | 1 |
| 13 | Detector development at the Back-n white neutron source显示文摘Purpose Back-n is a white neutron beamline at China spallation neutron source,which was established in the year of 2018.It is a powerful facility for nuclear data measurement,neutron detector calibration,and radiation effect research.Method A series of detectors were built for different experiments,including beam monitoring,beam profile measurement,neutron induced secondaries(fission fragments,light charged particles and gamma)cross section measurement,and neutron resonance radiography,etc.A common digitization electronics and a cluster-based DAQ were developed for these detector systems.Most detectors have been employed at Back-n and serviced for experiments from the beginning of the beamline running.Results and conclusion As an overview of detectors of Back-n,the details of the detector design and the experiment performing are described in this paper.Some developing systems,e.g.,MTPC and B-MCP,are also included. | Fan Ruirui Li Qiang Bao Jie Li Yang Liu Rong Jiang Wei Ren Jie Zhang Qiwei Cao Ping Gu Minhao Ren Zhizhou Yi Han Tang Jingyu An Qi Bai Haofan Bai Jiangbo Chen Qiping Chen Yonghao Chen Zhen Cui Zengqi Fan Anchuan Feng Changqing Feng Fanzhen Gao Keqing Han Changcai Han Zijie He Guozhu He Yongcheng Hong Yang Hu Yiwei Huang Hanxiong Jia Weihua Jiang Haoyu Jiang Zhijie Jin Zhengyao Kang Ling Li Bo Li Chao Li Gong Li Jiawen Li Xiao Liu Jie Liu Shubin Luan Guangyuan Ning Changjun Qi Binbin Ruan Xichao Song Zhaohui Sun Kang Tan Zhixin Tang Shengda Wang Pengcheng Wang Zhaohui Wen Zhongwei Wu Xiaoguang Wu Xuan Xie Likun Yang Yiwei Yu Yongji Zhang Guohui Zhang Linhao Zhang Mohan Zhang Xianpeng Zhang Yuliang Zhang Yue Zhang Zhiyong Zhao Maoyuan Zhou Luping Zhou Zhihao Zhu Kejun The CSNS Back-n Collaboration | 2023 | Radiation Detection Technology and Methods2023，7，2： | 0 |
| 14 | Preparation and Superconducting Properties of F-doped SmO_(1-x)F_xFeAs显示文摘Here we report the fabrication and superconductivity of the iron-based arsenic oxide SmO1-xFxFeAs compound.X-ray diffraction(XRD) results prove that the lattice parameters a and c decrease systematically with increasing x in between 00.35 the a and c increase with the decrease of x in the SmO1-xFxFeAs.The critical temperature(Tc) increases with increasing x in between 0.15≤x≤0.3,while x>0.3 the Tc decreases with the increase of x.It is found that at x=0.3 SmO0.7F0.3FeAs has the highest onset resistivity transition temperature of 55.5 K.The critical current density(Jc) value at 10 K for the obtained SmO0.7F0.3FeAs is 1.3×10 5 A/cm2(0T).Meanwhile one can estimates Hc2(0) from the slope of the Hc2(T) curve at T =Tc(HC2 is the upper critical field),and for the 90% normal-state resistivity(ρn) criterion(Tc=55 K),Hc2(0) is determined to be ~253 T. | Zhiyong Liu Hongli Suo Lin Ma Min Liu Zhiping Wu Zhichao Guo Luping Han Meiling Zhou | 2011 | Journal of Materials Science & Technology2011，27，3： | 0 |
| 15 | The construction of OPA1 overexpression plasmid and the cell model显示文摘The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established. | Ma Haojie Gai Cong Feng Wandi Cheng Cuicui Zhou Mengqi Zhang Jinkun Zhang Yuxin Yang Luping Guo Zhenyu Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 16 | Effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in PD cell model显示文摘Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease drugs.The PD cell model was constructed by applying MPP+to SH-SY5Y cells for 48 h,and they were treated with low,.medium and high concentrations of Timosaponin B-Ⅱ. | Zhou Mengqi Gai Cong Feng Wandi Ma Haojie Cheng Cuicui Zhao Haihong Zhang Yuxin Zhang Jinkun Yang Luping Sun Hongmei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 17 | CORRECTION:Correction to:Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘In the original version of Fig.41 and 4J,the cutoff lines were accidently shifted during figure layout.Figures 41 and 4J are re-drawn and provided below. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 0 |
| 18 | Ore-forming fluid characteristics and ore-forming materials source of the Tudimiaogou – Yindongshan lead-zinc polymetallic orefield, west Henan显示文摘1 Introduction The Tudimiaogou-Yindongshan lead-zinc polymetallic orefield is located in the Tudimiaogou-Weimoshi lead and zinc silver polymetallic metallogenic belt.The belt is an important part of southwestern Henan lead and | TAN Huajie SHAO Yongjun ZHENG Minghong ZHOU Houdi ZHANG Jiandong PENG Guangxiong Cheng Luping | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
| 19 | Comparison of the effectiveness and pregnancy outcomes of labor induction with dinoprostone or single-balloon catheter in term nulliparous women with borderline oligohydramnios显示文摘Backgrounds:At present,there is no consensus on the induction methods in term pregnancy with borderline oligohydramnios.This study aimed to compare the effectiveness and pregnancy outcomes of labor induction with dinoprostone or single-balloon catheter(SBC)in term nulliparous women with borderline oligohydramnios.Methods:We conducted a retrospective cohort study from January 2016 to November 2018.During the study period,a total of 244 cases were enrolled.Of these,103 cases were selected for induction using dinoprostone and 141 cases were selected for induction with SBC.The pregnancy outcomes between the two groups were compared.Primary outcomes were successful vaginal delivery rates.Secondary outcomes were maternal and neonatal adverse events.Multivariate logistic regression was used to assess the risk factors for vaginal delivery failure in the two groups.Results:The successful vaginal delivery rates were similar between the dinoprostone group and the SBC group(64.1%[66/103]vs.59.6%,[84/141]P=0.475),even after adjustment for potential confounding factors(adjusted odds ratio[aOR]:1.07,95%confidence interval[CI]:0.57-2.00,P=0.835).The incidence of intra-amniotic infection was lower in the dinoprostone group than in the SBC group(1.9%[2/103]vs.7.8%[11/141],P<0.001),but the presence of non-reassuring fetal heart rate was higher in the dinoprostone group than in the SBC group(12.6%[13/103]vs.0.7%,[1/141]P<0.001).Multivariate logistic regression showed that nuchal cord was a risk factor for vaginal delivery failure after induction with dinoprostone(aOR:6.71,95%CI:1.96-22.95).There were three factors related to vaginal delivery failure after induction with SBC,namely gestational age(aOR:1.51,95%CI:1.07-2.14),body mass index(BMI)>30 kg/m^(2)(aOR:2.98,95%CI:1.10-8.02),and fetal weight>3500 g(aOR:2.49,95%CI:1.12-5.50).Conclusions:Term nulliparous women with borderline oligohydramnios have similar successful vaginal delivery rates after induction with dinoprostone or SBC,with their advantages and disadvantages.In women with nuchal cord,the risk of vaginal delivery failure is increased if dinoprostone is used in the induction of labor.BMI>30 kg/m^(2),large gestational age,and estimated fetal weight>3500 g are risk factors for vaginal delivery failure after induction with SBC. | Yongqing Zhang Luping Chen Guohui Yan Menglin Zhou Zhengyun Chen Zhaoxia Liang Danqing Chen | 2022 | Chinese Medical Journal2022，，6： | 0 |
| 20 | Differentiating and Subdividing for Concept of Earnings Management显示文摘 | Luping Wang Wei Zhou | 2006 | Journal of Modern Accounting and Auditing2006，2，2： | 0 |
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