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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses显示文摘WD40 repeat-containing proteins(WD40 proteins)serve as versatile scaffolds for protein-protein interac-tions,modulating a variety of cellular processes such as plant stress and hormone responses.Here we report the identification of a WD40 protein,XIW1(for XPO1-interacting WD40 protein 1),which positively regulates the abscisic acid(ABA)response in Arabidopsis.XIW1 is located in the cytoplasm and nucleus.We found that it interacts with the nuclear transport receptor XPO1 and is exported by XPO1 from the nucleus.Mutation of XIW1 reduces the induction of ABA-responsive genes and the accumulation of ABA Insensitive 5(ABI5),causing mutant plants with ABA-insensitive phenotypes during seed germination and seedling growth,and decreased drought stress resistance.ABA treatment upregulates the expression of XIW1,and both ABA and abiotic stresses promote XIW1 accumulation in the nucleus,where it interacts with ABI5.Loss of XIW1 function results in rapid proteasomal degradation of ABI5.Taken together,these findings suggest that XIW1 is a nucleocytoplasmic shuttling protein and plays a positive role in ABA responses by interacting with and maintaining the stability of ABI5 in the nucleus. | Xuezhong XU Wang Wan Guobin Jiang Yue Xi Haijian Huang Jiajia Cai Yanan Chang Cheng-Guo Duan Satendra KMangrauthia Xinxiang Peng Jian-Kang Zhu Guohui Zhu | 2019 | Molecular Plant2019，12，12： | 8 |
| 3 | Response of glutamine synthetase isoforms to nitrogen sources in rice ( Oryza sativa L.) roots显示文摘 | Chufu Zhang Shaobing Peng Xinxiang Peng Arlene Q. Chavez John Bennett | 1997 | Plant Science1997，，2： | 2 |
| 4 | Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice显示文摘Mutations in the photorespiration pathway display a lethal phenotype in atmospheric air,which can be fully recovered by elevated CO2.An exception is that mutants of peroxisomal hydroxypyruvate reductase(HPR1)do not have this phenotype,indicating the presence of cytosolic bypass in the photorespiration pathway.In this study,we constructed overexpression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice(Oryza sativa L.cv.Zhonghua 11).Results from reverse transcription-polymerase chain reaction(RT-PCR),Western blot,and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity,which is the same for HPR2.However,metabolite analysis and the serine glyoxylate aminotransferase(SGAT)activity assay showed that the metabolitefiux of photorespiration was disturbed in RNAi lines of both HPR genes.Furthermore,HPR1 and HPR2proteins were located to the peroxisome and cytosol,respectively,by transient expression experiment.Double Research mutant hpr1 hpr2 was generated by crossing individual mutant of hpr1 and hpr2.The phenotypes of all transgenic lines were determined in ambient air and CO2-elevated air.The phenotype typical of photorespiration mutants was observed only where activity of both HPR1 and HPR2 were downregulated in the same line.Thesefindings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1and OsHPR2 are involved in photorespiratory metabolism in rice. | Nenghui Ye Guozhen Yang Yan Chen Chan Zhang Jianhua Zhang Xinxiang Peng | 2014 | Journal of Integrative Plant Biology2014，56，2： | 2 |
| 5 | Identification of Rice Al-responsive Genes by Semi-quantitative Polymerase Chain Reaction using Sulfite Reductase as a Novel Endogenous Control显示文摘Based on the evidence that Al resistance is an inducible process and rice is an Al-resistant crop,identification of Al-responsive genes from rice may help to further clone Al-resistant genes in plants.Semi-quantitative and real-time polymerase chain reaction (PCR) is widely applied in gene transcriptional analyses,particularly for those genes with low transcript abundance.Normalization with proper endogenous control (EC) genes is critical for these two approaches in terms of reliability and precision.We first noticed that the expression of several commonly-used EC genes was depressed under Al stress,while sulfite reductase gene (SR) was stable throughout the Al treatment.The reliability of SR as an EC gene was further tested by analyzing the expression of a number of genes in response to Al challenge.Except for the consistent results obtained for the four previously-identified genes,nine additional genes were newly defined as Al-responsive in this study.Collectively,our results suggest that SR can be used as a novel EC gene for semi-quantitative and real-time PCR analysis of Al responsive genes,and that activated transport of silicon and stimulated metabolism of carotenoid and terpenoid could be involved in Al resistance in rice plants. | Jianjun Zhang Ying Yin Yuqi Wang Xinxiang Peng | 2010 | Journal of Integrative Plant Biology2010，52，5： | 1 |
| 6 | Transcriptomic analysis reveals the defense mechanisms of citrus infested with Diaphorina citri显示文摘Huanglongbing(HLB) is a devastating disease that has led to an acute crisis for growers of citrus, one of the world's most important fruit crops. The phloem-feeding Asian citrus psyllid(ACP), Diaphorina citri, is the main pest at the new shoot stage and is the only natural vector of HLB pathogenic bacteria. Little is known about how plants perceive and defend themselves from this destructive pest. Here, we characterized changes in the expression of various genes in citrus plants that were continuously infested by D. citri for different durations(12, 24, and 48 h). A total of 5 219 differentially expressed genes(DEGs) and 643 common DEGs were identified across all time points. Several pathways related to defense were activated, such as peroxisome, alpha-linolenic acid metabolism, and phenylpropanoid and terpenoid biosynthesis, and some pathways related to growth and signal transduction were suppressed in response to D. citri infestation. The expression of genes including kinases(CML44, CIPK6, and XTH6), phytohormones(SAMT, LOX6, and NPR3), transcription factors(bHLH162, WRKY70, and WRKY40), and secondary metabolite synthesis-related genes(PAL, 4CL2, UGT74B1 and CYP82G1) was significantly altered in response to D. citri infestation. The findings of this study greatly enhance our understanding of the mechanisms underlying the defense response of citrus plants to D. citri infestation at the molecular level. Functional characterization of the candidate defense-related genes identified in this study will aid the molecular breeding of insect-resistant citrus varieties. | Xueli Sun Ting Yu Minliang Bin Chunhua Hu Fangcheng Bi Xinxiang Peng Ganjun Yi Xinxin Zhang | 2023 | Horticultural Plant Journal2023，9，3： | 1 |
| 7 | Organic acid exudation induced by phosphorus deficiency and/or aluminium toxicity in two contrasting soybean genotypes显示文摘 | Dengfeng Dong Xinxiang Peng Xiaolong Yan | 2004 | Physiologia Plantarum2004，122，： | 1 |
| 8 | In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis?×?C. dactylon) and their physiological responses to salt and drought stress显示文摘 | Shaoyun Lu Xinxiang Peng Zhenfei Guo Gengyun Zhang Zhongcheng Wang Congying Wang Chaoshu Pang Zhen Fan Jihua Wang | 2007 | Plant Cell Reports2007，，8： | 1 |
| 9 | Author Correction: 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘In the initial published version of this article, there was a mistake in the P value for the correlation between gene-expression changes and 5 hmC changes in tumors. The correct P value should be same as the P value shown in Fig. S6A: 9.8?×?10?6 (mistakenly shown as “9.8?×?106” in the main text). This correction does not affect the description of results or the conclusions of this study, since the range of P value is between 0 and 1. | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2019 | Cell Research2019，29，7： | 1 |
| 10 | Influence of traditional Chinese medicines on the in vivo metabolism of lopinavir/ritonavir based on UHPLC-MS/MS analysis显示文摘A fast,reliable,and cost-effective liquid chromatography-tandem mass spectrometry method was established to determine the effects of the traditional Chinese medicine employed to treat coronavirus disease 2019,namely,Lianhua Qingwen granules,Huoxiang Zhengqi capsules,Jinhua Qinggan granules,Shufeng Jiedu capsules,and Angong Niuhuang pills,on the pharmacokinetics of lopinavir/ritonavir in rats.Blood samples were prepared using the protein precipitation method and atazanavir was selected as the internal standard(IS).Separation was performed on an Agilent ZORBAX eclipse plus C18(2.1 mm×50 mm,1.8 μm)column using acetonitrile and water containing 0.1%formic acid as the mobile phase for gradient elution.The flow rate was 0.4 mL/min and the injection volume was 2 μL.Agilent Jet Stream electrospray ionization was used for mass spectrometry detection under positive ion multiple reaction monitoring mode at a transition of m/z 629.3→447.3 for lopinavir,m/z 721.3→296.1 for ritonavir,and m/z 705.4→168.1 for the IS.The method showed good linearity in the concentration range of 25→2500 ng/mL(r=0.9981)for lopinavir and 5e500 ng/mL(r=0.9984)for ritonavir.The intra-day and inter-day precision and accuracy were both within±15%.Items,such as dilution reliability and residual effect,were also within the acceptable limits.The method was used to determine the effects of five types of traditional Chinese medicines on the pharmacokinetics of lopinavir/ritonavir in rats.The pharmacokinetic results showed that the half-life of ritonavir in the groups administered Lianhua Qingwen granules and Huoxiang Zhengqi capsules combined with lopinavir/ritonavir was prolonged by approximately 1.5-to 2-fold relative to that in the control group.Similarly,the pharmacokinetic parameters of lopinavir were altered.Overall,the results of this study offer important theoretical parameters for the effective clinical use of five types of traditional Chinese medicines combined with lopinavir/ritonavir to reduce the occurrence of clinical adverse reactions. | Linlin Li Xinxiang Yu Dongmin Xie Ningning Peng Weilin Wang Decai Wang Binglong Li | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 1 |
| 11 | Laparoscopic Low Anterior Resection and Eversion Technique Combined With a Nondog Ear Anastomosis for Mid- and Distal Rectal Neoplasms: A Preliminary and Feasibility Study显示文摘 | Changhua Zhuo Lei Liang Mingang Ying Qingguo Li Dawei Li Yiwei Li Junjie Peng Liyong Huang Sanjun Cai Xinxiang Li | 2015 | Medicine2015，，50： | 1 |
| 12 | Response of glutamine synthetase isoforms to nitrogen sources in rice ( Oryza sativa L.) roots显示文摘 | Chufu Zhang Shaobing Peng Xinxiang Peng Arlene Q. Chavez John Bennett | 1997 | Plant Science1997，，2： | 1 |
| 13 | The nuclear export receptor OsXPO1 is required for rice development and involved in abiotic stress responses显示文摘The transport of proteins to and from the nucleus is necessary for many cellular processes and is one of the ways plants respond to developmental signals and environmental stresses.Nucleocytoplasmic trafficking of proteins is mediated by the nuclear transport receptor(NTR).Although NTR has been extensively studied in humans and Arabidopsis,it has rarely been identified and functionally characterized in rice.In this study,we identified exportin 1 in rice(OsXPO1)as a nuclear export receptor.OsXPO1shares high protein identity with its functional homologs in Arabidopsis and other organisms.OsXPO1localized to both the nucleus and the cytoplasm,directly interacted with the small GTPases OsRAN1and OsRAN2 in the nucleus,and mediated their nuclear export.Loss-of-function osxpo1 mutations were lethal at the seedling stage.Suppression of OsXPO1 expression in RNA interference lines produced multifaceted developmental defects,including arrested growth,premature senescence,abnormal inflorescence,and brown and mouth-opened spikelets.Overexpression of OsXPO1 in rice reduced plant height and seed-setting rate,but increased plant tolerance in response to PEG-mimicked drought stress and salt stress.These results indicate that OsXPO1 is a nuclear export receptor and acts in regulating plant development and abiotic stress responses. | Qiufei Peng Jieyu Qiu Xintong Li Xuezhong Xu Xinxiang Peng Guohui Zhu | 2023 | The Crop Journal2023，11，1： | 0 |
| 14 | Acute Toxicity of Four Organophosphorus Pesticide Products显示文摘Acute toxicity of phoxim,acephate,isofenphos-methyl and isocarbophos on male SD rats of clean grade was carried out by gastric lavage method at room temperature of 18℃. These rats are 4 to 5 months old with body weight of 180 to 220 kg. The results indicate that the orders of the toxicity of these four pesticides on SD rats are isofenphos-methyl,isocarbophos,acephate,phoxim. We found that the median lethal concentration of phoxim in 24 h,48 h,72 h and 96 h is 3. 892 g /kg,3. 051 g /kg,2. 618 g /kg and 2. 458 g /kg respectively; the median lethal concentration of isofenphos-methyl in 24 h,48 h,72 h and 96 h is 0. 015 g /kg,0. 013g /kg,0. 012g /kg and 0. 011 g /kg respectively; the median lethal concentration of isocarbophos in 24 h,48 h,72 h and 96 h is 0. 049 g /kg,0. 046 g /kg,0. 043 g /kg,0. 041 g /kg respectively; and the median lethal concentration of acephate in 24 h,48 h,72 h and 96 h is 0. 137 g /kg,0. 113 g /kg,0. 100 g /kg,0. 085 g /kg respectively. Finally,we evaluated the characteristics of toxicity effect and safe concentration of these pesticides to SD rats. | LI Peng 1 ,YIN Ya-ling 2 ,WANG Qian-qian 1 ,ZHAO Fan-rong 1 ,PAN Guo-pin 1 ,ZHANG Yu-lin 1 1. School of Pharmacy,Xinxiang Medical University,Xinxiang 453003,China 2. Medical Teaching Institute,Xinxiang Medical University,Xinxiang 453003,China | 2012 | Asian Agricultural Research2012，4，5： | 0 |
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