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101篇 您的检索式:作者名="Zhenfei"
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1Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li 2015Cell Research2015,25,1:37
2Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection显示文摘YuxuanWu Li Yang Jinsong Li Cuiqing Zhong Zhenfei Xie Qi Yin Rui Dong Suming Yang 2016Cell Research2016,26,1:13
3Reverse Genetic Identification of CRN1 and its Distinctive Role in Chlorophyll Degradation in Arabidopsis显示文摘Recent identification of NYE1/SGR1 brought up a new era for the exploration of the regulatory mechanism of Chlorophyll (Chl) degradation.Cluster analysis of senescence associated genes with putative chloroplast targeting sequences revealed several genes sharing a similar expression pattern with NYE1.Further characterization of available T-DNA insertion lines led to the discovery of a novel stay-green gene CRN1 (Co-regulated with NYE1).Chl breakdown was significantly restrained in crn1-1 under diversified senescence scenarios,which is comparable with that in acd1-20,but much more severe than that in nye11.Notably,various Chl binding proteins,especially trimeric LHCP II,were markedly retained in crn1-1 four days after dark-treatment,possibly due to a lesion in disassociation of protein-pigment complex.Nevertheless,the photochemical efficiency of PSII in crn1-1 declined,even more rapidly,two days after dark-treatment,compared to those in Col-0 and nye1-1.Our results suggest that CRN1 plays a crucial role in Chl degradation,and that loss of its function produces various side-effects,including those on the breakdown of Ch-protein complex and the maintenance of the residual photosynthetic capability during leaf senescence.Guodong Ren Qian Zhou Shouxin Wu Yufan Zhang Lingang Zhang Jirong Huang Zhenfei Sun Benke Kuai 2010Journal of Integrative Plant Biology2010,52,5:8
4Dosage effect of multiple genes accounts for multisystem disorder of myotonic dystrophy type 1显示文摘Multisystem manifestations in myotonic dystrophy type 1(DM1)may be due to dosage reduction in multiple genes induced by aberrant expansion of CTG repeats in DMPK,including DMPK,its neighboring genes(SIX5 or DMWD)and downstream MBNLh However,direct evidence is lacking.Here,we develop a new strategy to generate mice carrying multigene heterozygous mutations to mimic dosage reduction in one step by injection of haploid embryonic stem cells with mutant Dmpk,Six5 and Mbnll into oocytes.The triple heterozygous mutant mice exhibit adult-onset DM1 phenotypes.With the additional mutation in Dmwd,the quadruple heterozygous mutant mice recapitulate many major manifestations in congenital DM1.Moreover,muscle stem cells in both models display reduced sternness,providing a unique model for screening small molecules for treatment of DM1.Our results suggest that the complex symptoms of DM1 result from the reduced dosage of multiple genes.Qi Yin Hongye Wang Na Li Yifu Ding Zhenfei Xie Lifang Jin Yan Li Qiong Wang Xinyi Liu Liuqing Xu Qing Li Yongjian Ma Yanbo Cheng Kai Wang Cuiqing Zhong Qian Yu Wei Tang Wanjin Chen Wenjun Yang Fan Zhang Chen Ding Lan Bao Bin Zhou Ping Hu Jinsong Li 2020Cell Research2020,30,2:7
5Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine显示文摘Mesenchymal stem cell(MSC)-mediated immunomodulation has been harnessed for the treatment of human diseases,but its underlying mechanism has not been fully understood.Dead cells,including apoptotic cells have immunomodulatory properties.It has been repeatedly reported that the proportion of nonviable MSCs in a MSC therapeutic preparation varied from 5-50%in the ongoing clinical trials.It is conceivable that the nonviable cells in a MSC therapeutic preparation may play a role in the therapeutic effects of MSCs.We found that the MSC therapeutic preparation in the present study had about 5%dead MSCs(DMSCs),characterized by apoptotic cells.Namely,1×10^(6) MSCs in the preparation contained about 5×10^(4) DMSCs.We found that the treatment with even 5×10^(4) DMSCs alone had the equal therapeutic effects as with 1×10^(6) MSCs.This protective effect of the dead MSCs alone was confirmed in four mouse models,including concanavalin A(ConA)-and carbon tetrachloride(CCI4)-induced acute liver injury,LPS-induced lung injury and spinal cord injury.We also found that the infused MSCs died by apoptosis in vivo.Furthermore,the therapeutic effect was attributed to the elevated level of phosphatidylserine(PS)upon the injection of MSCs or DMSCs.The direct administration of PS liposomes(PSLs)mimic apoptotic cell fragments also exerted the protective effects as MSCs and DMSCs.The Mer tyrosine kinase(MerTK)deficiency or the knockout of chemokine receptor C-C motif chemokine receptor 2(CCR2)reversed these protective effects of MSCs or DMSCs.These results revealed that DMSCs alone in the therapeutic stem cell preparation or the apoptotic cells induced in vivo may exert the same immunomodulatory property as the'living MSCs preparation'through releasing PS,which was further recognized by MerTK and participated in modulating immune cells.Xuemei He Weiqi Hong Jingyun Yang Hong Lei Tianqi Lu Cai He Zhenfei Bi Xiangyu Pan Yu Liu Lunzhi Dai Wei Wang Canhua Huang Hongxin Deng Xiawei Wei 2021Signal Transduction and Targeted Therapy2021,6,8:6
6An Efficient Method for Estimating Capability Curve of Virtual Power Plant显示文摘To incorporate the operating constraints of a virtual power plant(VPP)in transmission-level operation and market clearing,the concept of the VPP capability curve(VPP-CC)is proposed which explicitly characterizes the allowable range of active and reactive power outputs of a VPP.A two-step projection-based calculation framework is proposed to approximate the VPP-CC by the convex hull of critical points on its perimeter.The output of the proposed algorithm is concise and can be easily incorporated in the existing system operation and market clearing.Case studies based on the IEEE 33 and 123 test feeders show the computational efficiency of the proposed method outperforms existing methods by 4~7 times.Additionally,many fewer inequalities are needed to depict the VPP-CC while achieving the comparative approximation accuracy compared to sampling-based methods,which will relieve the communication and computation burden.Zhenfei Tan Haiwang Zhong Xuanyuan Wang Honghai Tang 2022CSEE Journal of Power and Energy Systems2022,8,3:5
7Treatment of Helicobacter pylori-associated gastritis using traditional Chinese medicine显示文摘Helicobacter pylori is a pathogenic bacterium with strong pathogenicity, which can induce chronic gastritis and peptic ulcer in addition to causing gastric mucosal atrophy and intestinal metaplasia after longterm infection. It is even associated closely with the occurrence of gastric cancer and mucosa-associated lymphoid tissue lymphoma. Recently, the incidence of H. pylori-associated gastritis has increased rapidly worldwide, especially in China. The prevention and treatment of H. pylori infection has become an urgent issue to be resolved by the medical community. Traditional Chinese medicine and the combination of traditional Chinese medicine and Western medicine have recently shown unique advantages in the treatment of H. pylori–associated gastritis. The progress in this area is summarized in this review.Mengjiao Hu Zhenfei Wang 2018国际感染病学(电子版)2018,7,1:4
8Digital Watermarking Algorithm Based on Wavelet Transform and Neural Network显示文摘An effective blind digital watermarking algorithm based on neural networks in the wavelet domain is presented. Firstly, the host image is decomposed through wavelet transform. The significant coefficients of wavelet are selected according to the human visual system (HVS) characteristics. Watermark bits are added to them. And then effectively cooperates neural networks to learn the characteristics of the embedded watermark related to them. Because of the learning and adaptive capabilities of neural networks, the trained neural networks almost exactly recover the watermark from the watermarked image. Experimental results and comparisons with other techniques prove the effectiveness of the new algorithm.WANG Zhenfei ZHAI Guangqun WANG Nengchao 2006Wuhan University Journal of Natural Sciences2006,11,6:4
9Texas Electric Power Crisis of 2021 Warns of a New Blackout Mechanism显示文摘The Texas electric power crisis that occurred in February 2021 has drawn great attention internationally due to its severity and for not having been foreseen.In this rapid communication,we classify the 2021 Texas electric power crisis as an energy insufficiency-caused power crisis,which alerts of a new blackout mechanism.Different from capacity insufficiencycaused power crises in the past,the Texas electric power crisis of 2021 directly resulted from the long-duration extreme cold weather as well as fundamentally from the insufficiency of sustainable supply capability of energy.We begin this paper with a brief retrospect of the event and its consequences.Definitions of energy/capacity insufficiency-caused power crises are given,as well as an overview of the supply and demand during the event,based on realistic operation data.Quantitative simulations are then conducted to reveal the underlying reasons for the power crisis and reveal how to better prepare for the future.Finally,several insights and suggestions on handling the new mode of blackout in the future are proposed and discussed.Guanglun Zhang Haiwang Zhong Zhenfei Tan Tong Cheng Qing Xia Chongqing Kang 2022CSEE Journal of Power and Energy Systems2022,8,1:4
10Systematic centroid error compensation for the simple Gaussian PSF in an electronic star map simulator显示文摘Simulated star maps serve as convenient inputs for the test of a star sensor, whose standardability mostly depends on the centroid precision of the simulated star image, so it is necessary to accomplish systematic error compensation for the simple Gaussian PSF(or SPSF, in which PSF denotes point spread function). Firstly, the error mechanism of the SPSF is described, the reason of centroid deviations of the simulated star images based on SPSF lies in the unreasonable sampling positions(the centers of the covered pixels) of the Gaussian probability density function. Then in reference to the IPSF simulated star image spots regarded as ideal ones, and by means of normalization and numerical fitting, the pixel center offset function expressions are got, so the systematic centroid error compensation can be executed simply by substituting the pixel central position with the offset position in the SPSF. Finally, the centroid precision tests are conducted for the three big error cases of Gaussian radius r = 0.5, 0.6, 0.671 pixel, and the centroid accuracy with the compensated SPSF(when r = 0.5) is improved to 2.83 times that of the primitive SPSF, reaching a 0.008 pixel error, an equivalent level of the IPSF. Besides its simplicity, the compensated SPSF further increases both the shape similarity and the centroid precision of simulated star images, which helps to improve the image quality and the standardability of the outputs of an electronic star map simulator(ESS).Wang Haiyong Wang Yonghai Li Zhifeng Song Zhenfei 2014Chinese Journal of Aeronautics2014,27,4:3
11Microstructure and Mechanical Behavior of FeNiCoCr and FeNiCoCrMn High-Entropy Alloys Fabricated by Powder Metallurgy显示文摘The present work reports a systematic investigation on phase evolution,microstructure and microstructure-property relationship of two typical face-centered cubic(FCC)structured high-entropy alloys(HEAs),FeNiCoCr and FeNiCoCrMn,prepared via mechanical alloying(MA)followed by spark plasma sintering(SPS).Following 50 h of MA,the two HEAs consisted of a mixture of FCC and body-centered cubic phases.Following SPS,the bulk FeNiCoCr alloy showed a primary FCC phase with a small amount of Cr_(23)C_(6) and Cr_(2)O_(3) contaminants,while the bulk FeNiCoCrMn alloy was composed of a primary FCC phase with some(Cr,Mn)23C6 and MnCr_(2)O_(4) contaminants.The average grain size of the primary FCC phase in the bulk FeNiCoCr alloy was~416 nm,while that of the primary FCC phase in the bulk FeNiCoCrMn alloy was~547 nm.The yield strength,compressive strength and strain-to-failure of the bulk FeNiCoCr alloy are 1525 MPa,1987 MPa and 24.4%,respectively,whereas those of the bulk FeNiCoCrMn alloy are 1329 MPa,1761 MPa and 21.9%,respectively.It suggests that the bulk FeNiCoCrMn exhibited lower strength and plasticity in comparison with the bulk FeNiCoCr alloy.Clearly,the smaller grain size of the primary FCC phase in the FeNiCoCr alloy is mainly responsible for the better mechanical performance.Chenliang Chu Weiping Chen Zhen Chen Zhenfei Jiang Hao Wang Zhiqiang Fu 2021Acta Metallurgica Sinica(English Letters)2021,34,4:3
12Analysis of microcrystal formation in DS-silicon ingot显示文摘The DS(directional solidification) polycrystalline silicon ingot is the most important photovoltaic material today,and the conversion efficiency of solar cells is affected by the morphology and organization of the crystal.Uniform grains with larger size are conducive to get high-quality wafer,so improving the cell conversion efficiency.However,grains sizes that are less than 1 mm2 can be observed frequently in the central district of mc-Si ingots,which bring negative effect to the quality of the mc-Si ingot and decrease the electrical performance of wafer.In this paper,we make an attempt to explain the formation mechanism and influence factors of microcrystal in mc-Si ingot with computer simulation technology and theory of component supercooling.It was found that:to avoid production of microcrystal,it's better to increase the value of G/V(V is the growth rate and G is the near-interface temperature gradient),strengthen the melt convection front in the solidification interface and keep a fairly flat solid/melt interface in producing mc-Si ingot.ZHANG ZhiQiang HUANG Qiang HUANG ZhenFei LI BiWu CHEN Xue 2011Science China(Technological Sciences)2011,54,6:3
13Lamin-like Proteins Negatively Regulate Plant Immunity through NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 and NONEXPRESSOR OF PR 3ENES1 in Arabidopsis thaliana显示文摘Tongtong Guo Xuegao Mao Hui Zhang Yu Zhang Mengdi Fu Zhenfei Sun Peng Kuai Yonggen Lou Yuda Fang 2017Molecular Plant2017,10,10:3
14The mechanism of HBx protein to promote the initiation and progression of hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC) is the sixth most common malignancy worldwide and the third most common cause of death from cancer, after lung and stomach cancer. Hepatitis B virus(HBV) infection is closely related to HCC and is a major cause of HCC. HBV is a lysogenic virus of the hepadnavirus family. Its genome presents a slack, ring-like, double-chain structure, containing four open reading frames. The X region encodes the product HBV X protein(HBx), which is a multifunctional regulatory protein that plays an important role in intracellular signal transduction, viral genome replication and transcription, cell proliferation and apoptosis, cell cycle progression, protein degradation, and genetic stability of hepatocytes. This article summarizes the recent research on the mechanism of promotion of initiation and progression of HCC by HBx protein.Muyao Zhang Xing Wei Zhenfei Wang 2018国际感染病学(电子版)2018,7,1:2
15Cloning and characterization of a novel CBL-interacting protein kinase from maize显示文摘Jinfeng Zhao Zhenfei Sun Jun Zheng Xiying Guo Zhigang Dong Junling Huai Mingyue Gou Junguang He Yongsheng Jin Jianhua Wang Guoying Wang 2009Plant Molecular Biology2009,,6:2
16Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19.Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei 2022Cellular & Molecular Immunology2022,19,5:2
17Organophosphate esters cause thyroid dysfunction via multiple signaling pathways in zebrafish brain显示文摘Organophosphate esters(OPEs)are widespread in various environmental media,and can disrupt thyroid endocrine signaling pathways.Mechanisms by which OPEs disrupt thyroid hormone(TH)signal transduction are not fully understood.Here,we present in vivo-in vitro-in silico evidence establishing OPEs as environmental THs competitively entering the brain to inhibit growth of zebrafish via multiple signaling pathways.OPEs can bind to transthyretin(TTR)and thyroxine-binding globulin,thereby affecting the transport of TH in the blood,and to the brain by TTR through the blood-brain barrier.When GH3 cells were exposed to OPEs,cell proliferation was significantly inhibited given that OPEs are competitive inhibitors of TH.Cresyl diphenyl phosphate was shown to be an effective antagonist of TH.Chronic exposure to OPEs significantly inhibited the growth of zebrafish by interfering with thyroperoxidase and thyroglobulin to inhibit TH synthesis.Based on comparisons of modulations of gene expression with the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases,signaling pathways related to thyroid endocrine functions,such as receptor-ligand binding and regulation of hormone levels,were identified as being affected by exposure to OPEs.Effects were also associated with the biosynthesis and metabolism of lipids,and neuroactive ligand-receptor interactions.These findings provide a comprehensive understanding of the mechanisms by which OPEs disrupt thyroid pathways in zebrafish.Zhenfei Yan Chenglian Feng Xiaowei Jin Fangkun Wang Cong Liu Na Li Yu Qiao Yingchen Bai Fengchang Wu John P.Giesy 2022Environmental Science and Ecotechnology2022,,4:2
18A comparison of robot-assisted and fluoroscopy-assisted kyphoplasty in the treatment of multi-segmental osteoporotic vertebral compression fractures显示文摘Osteoporotic vertebral compression fracture(OVCF)has become a major public health issue that becomes more pressing with increasing global aging.Percutaneous kyphoplasty(PKP)is an effective treatment for OVCF.Robot-assisted PKP has been utilized in recent years to improve accuracy and reduce complications.However,the effectiveness of robot-assisted PKP in the treatment of multi-segmental OVCF has yet to be proved.This study was designed to compare the efficacy of robot-assisted and conventional fluoroscopy-assisted multi-segmental PKP.A total of 30 cases with multi-segmental OVCF between April 2019 and April 2021 were included in this study.Fifteen cases were assigned to the robot-assisted PKP group(robot group)and 15 cases to the conventional fluoroscopy-assisted PKP group(conventional fluoroscopy group).The number of fluoroscopic exposures,fluoroscopic dose,operation time,cement leakage rate,visual analog scale(VAS)score,vertebral kyphosis angle(VKA),and height of fractured vertebral body(HFV)were compared between the 2 groups.The number of fluoroscopic exposures,fluoroscopic doses,and cement leakage rates in the robot group were lower than in the conventional fluoroscopy group(P<0.05)while the operative time in the robot group was longer than in the conventional fluoroscopy group(P<0.05).VAS score and VKA were decreased and HFV was increased after surgery in both groups(P<0.05).Therefore,robot-assisted PKP for the treatment of multi-segmental OVCF can reduce the number of fluoroscopic exposures,fluoroscopic doses,and cement leakage compared to conventional treatment.As such,robot-assisted PKP has good application prospects and is potentially more effective in the treatment of multi-segmental OVCF.Qingqing Li Chaoqin Wu Zhenfei Huang Jiang Cao Jie Chang Guoyong Yin Lipeng Yu Xiaojian Cao Tao Sui 2022The Journal of Biomedical Research2022,36,3:2
19Toxicity mechanisms and bioavailability of copper to fish based on an adverse outcome pathway analysis显示文摘Copper(Cu)exists in a variety of forms in different aquatic environments,and affects their bioavailability.In this study we provide a systematic review on toxicity of Cu which focuses on identifying evidence in the mechanisms of Cu toxicity,and apply an adverse outcome pathway(AOP)analysis to identify multiple potential mechanisms and their interactions of Cu toxicity to fish.This analysis process included the mechanisms of behavior toxicant,oxidative toxicant,ion regulation disruption toxicity,as well as endocrine disruption toxicity.It was found that at low levels of Cu exposure,swimming,avoid predators,locating prey and other sensory functions will be impaired,and the organism will suffer from metabolic alkalosis and respiratory acidosis following the inhibition of the carbonic anhydrase active.The main pathway of acute toxicity of Cu to fish is the inhibition of the Na^(+)/K^(+)-ATPase enzyme,and lead to reduced intracellular sodium absorption,as well as Cu-induced increased cell permeability,in turn resulting in increased sodium ion loss,leading to cardiovascular collapse and respiratory insufficiency.The endocrine disruption toxicity of Cu to fish caused growth inhibition and reproductive reduction.In addition,there are several key pathways of Cu toxicity that are affected by hardness(e.g.,Ca^(2+))and intracellular DOC concentrations,including inhibiting Cu-induction,improving branchial gas exchange,altering membrane transport functions,decreasing Na+loss,and increasing Na+uptake.The results of the AOP analysis will provide a robust framework for future directed research on the mechanisms of Cu toxicity.Wei Liao Ziwei Zhu Chenglian Feng Zhenfei Yan Yajun Hong Daqing Liu Xiaowei Jin 2023Journal of Environmental Sciences2023,,5:1
20The properties of cementitions materials superlasticized with two superplasticizers based on aminosulfonate-phenol-formalde- hyde显示文摘Meishan Pei Zhenfei Wang Wenwei Li 2008Construction and Building Materials2008,22,:1
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