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| 1 | Nano-hydroxyapatite accelerates vascular calcification via lysosome impairment and autophagy dysfunction in smooth muscle cells显示文摘Vascular calcification(VC)is a common characteristic of aging,diabetes,chronic renal failure,and atherosclerosis.The basic component of VC is hydroxyapatite(HAp).Nano-sized HAp(nHAp)has been identified to play an essential role in the development of pathological calcification of vasculature.However,whether nHAp can induce calcification in vivo and the mechanism of nHAp in the progression of VC remains unclear.We discovered that nHAp existed both in vascular smooth muscle cells(VSMCs)and their extracellular matrix(ECM)in the calcified arteries from patients.Synthetic nHAp had similar morphological and chemical properties as natural nHAp recovered from calcified artery.nHAp stimulated osteogenic differentiation and accelerated mineralization of VSMCs in vitro.Synthetic nHAp could also directly induce VC in vivo.Mechanistically,nHAp was internalized into lysosome,which impaired lysosome vacuolar H+-ATPase for its acidification,therefore blocked autophagic flux in VSMCs.Lysosomal re-acidification by cyclic-3′,5′-adenosine monophosphate(cAMP)significantly enhanced autophagic degradation and attenuated nHAp-induced calcification.The accumulated autophagosomes and autolysosomes were converted into calcium-containing exosomes which were secreted into ECM and accelerated vascular calcium deposit.Inhibition of exosome release in VSMCs decreased calcium deposition.Altogether,our results demonstrated a repressive effect of nHAp on lysosomal acidification,which inhibited autophagic degradation and promoted a conversion of the accumulated autophagic vacuoles into exosomes that were loaded with undissolved nHAp,Ca^(2+),Pi and ALP.These exosomes bud off the plasma membrane,deposit within ECM,and form calcium nodules.Vascular calcification was thus accelerated by nHAP through blockage of autophagic flux in VSMCs. | Qi Liu Yi Luo Yun Zhao Pingping Xiang Jinyun Zhu Wangwei Jing Wenjing Jin Mingyao Chen Ruikang Tang Hong Yu | 2022 | Bioactive Materials2022，7，2： | 5 |
| 2 | S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling显示文摘Alcoholic liver disease(ALD) causes insulin resistance, lipid metabolism dysfunction, and inflammation. We investigated the protective effects and direct regulating target of S-allylmercaptocysteine(SAMC) from aged garlic on liver cell injury. A chronic ethanol-fed ALD in vivo model(the NIAAA model) was used to test the protective functions of SAMC. It was observed that SAMC(300 mg/kg, by gavage method) effectively ameliorated ALD-induced body weight reduction, steatosis,insulin resistance, and inflammation without affecting the health status of the control mice, as demonstrated by histological, biochemical, and molecular biology assays. By using biophysical assays and molecular docking, we demonstrated that SAMC directly targeted insulin receptor(INSR) protein on the cell membrane and then restored downstream IRS-1/AKT/GSK3 b signaling. Liver-specific knock-down in mice and siRNA-mediated knock-down in AML-12 cells of Insr significantly impaired SAMC(250 mmol/L in cells)-mediated protection. Restoration of the IRS-1/AKT signaling partly recovered hepatic injury and further contributed to SAMC’s beneficial effects. Continuous administration of AKT agonist and recombinant IGF-1 in combination with SAMC showed hepato-protection in the mice model.Long-term(90-day) administration of SAMC had no obvious adverse effect on healthy mice. We conclude that SAMC is an effective and safe hepato-protective complimentary agent against ALD partly through the direct binding of INSR and partial regulation of the IRS-1/AKT/GSK3 b pathway. | Pingping Luo Ming Zheng Rui Zhang Hong Zhang Yingxia Liu Wei Li Xiaoming Sun Qian Yu George LTipoe Jia Xiao | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 4 |
| 3 | Direct Ink Writing of Highly Conductive MXene Frames for Tunable Electromagnetic Interference Shielding and Electromagnetic Wave-Induced Thermochromism显示文摘The highly integrated and miniaturized next-generation electronic products call for high-performance electromagnetic interference(EMI)shielding materials to assure the normal operation of their closely assembled components.However,the most current techniques are not adequate for the fabrication of shielding materials with programmable structure and controllable shielding efficiency.Herein,we demonstrate the direct ink writing of robust and highly conductive Ti3C2Tx MXene frames with customizable structures by using MXene/AlOOH inks for tunable EMI shielding and electromagnetic wave-induced thermochromism applications.The as-printed frames are reinforced by immersing in AlCl_(3)/HCl solution to remove the electrically insulating AlOOH nanoparticles,as well as cross-link the MXene sheets and fuse the filament interfaces with aluminum ions.After freeze-drying,the resultant robust and porous MXene frames exhibit tunable EMI shielding efficiencies in the range of 25-80 dB with the highest electrical conductivity of 5323 S m−1.Furthermore,an electromagnetic wave-induced thermochromic MXene pattern is assembled by coating and curing with thermochromic polydimethylsiloxane on a printed MXene pattern,and its color can be changed from blue to red under the high-intensity electromagnetic irradiation.This work demonstrates a direct ink printing of customizable EMI frames and patterns for tuning EMI shielding efficiency and visualizing electromagnetic waves. | Xinyu Wu Tingxiang Tu Yang Dai Pingping Tang Yu Zhang Zhiming Deng Lulu Li Hao-Bin Zhang Zhong-Zhen Yu | 2021 | Nano-Micro Letters2021，13，10： | 3 |
| 4 | Clinical significance of extended high-risk human papillomavirus genotyping and viral load in cervical cancer and precancerous lesions显示文摘Persistent infections with specific high-risk human papillomavirus(HR-HPV)strains are the leading cause of cervical cancer and precancerous lesions.HPV-16 and HPV-18 are associated with more than 70%of cervical cancer.However,with recent widespread vaccination efforts against cervical cancer,the infection rates of HPV-16 and HPV-18 have decreased across all age groups,while the infection rates of other HR-HPV strains have increased.The non-16/18 HR-HPV strains play an important role in cervical lesions.These strains can be identified with extended genotyping,and the 2019 American Society for Colposcopy and Cervical Pathology(ASCCP)guidelines recommended an HPV-based testing to assess the risk of cervical disease in patients.We reviewed and analyzed the clinical benefits of applying extended HR-HPV genotyping,which was published by the International Agency for Research on Cancer(HPV-16,18,31,33,35,39,45,51,52,56,58,59,66,and 68),to cervical cancer screening.This review concluded that cervical cancer screening needs to include extended HR-HPV genotyping.The examination of extended HR-HPV genotyping in cervical intraepithelial lesions and cervical cancers can help guide clinical practices. | Pingping Su Jincheng Ma Lirui Yu Shuting Tang Pengming Sun | 2023 | Gynecology and Obstetrics Clinical Medicine2023，3，1： | 2 |
| 5 | The role of the MAP kinase−kinase protein StMKK1 in potato immunity to different pathogens显示文摘Mitogen-activated protein kinase(MAPK)cascades play important roles in plant immunity.Previously,we reported that the potato StMKK1 protein negatively regulates Nicotiana benthamiana resistance to Phytophthora infestans.However,the functions of StMKK1 in potato immunity are unknown.To investigate the roles of StMKK1 in potato resistance to different pathogens,such as the potato late-blight pathogen P.infestans,the bacterial wilt pathogen Ralstonia solanacearum,and the gray-mold fungal pathogen Botrytis cinerea,we generated StMKK1 transgenic lines and investigated the response of potato transformants to destructive oomycete,bacterial,and fungal pathogens.The results showed that overexpression and silencing of StMKK1 do not alter plant growth and development.Interestingly,we found that StMKK1 negatively regulated potato resistance to the hemibiotrophic/biotrophic pathogens P.infestans and R.solanacearum,while it positively regulated potato resistance to the necrotrophic pathogen B.cinerea.Further investigation showed that overexpression of StMKK1 suppressed potato pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)and salicylic acid(SA)-related responses,while silencing of StMKK1 enhanced PTI and SA-related immune responses.Taken together,our results showed that StMKK1 plays dual roles in potato defense against different plant pathogens via negative regulation of PTI and SA-related signaling pathways. | Xiaokang Chen Wenbin Wang Pingping Cai Ziwei Wang Tingting Li Yu Du | 2021 | Horticulture Research2021，8，1： | 2 |
| 6 | Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway显示文摘 | Huanlong Liu Xueyan Chen Yu Han Chaoyi Li Pingping Chen Yongjian Zhang Zhenhua Pan | 2013 | Biomedicine & Pharmacotherapy2013，，： | 2 |
| 7 | DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice显示文摘Arabinogalactan proteins(AGPs)are widely distributed in plant cells.Fasciclin-like AGPs(FLAs)belong to a subclass of AGPs that play important roles in plant growth and development.However,little is known about the biological functions of rice FLA.Herein,we report the identification of a male-sterile mutant of DEFECTIVE EXINE AND APERTURE PATTERNING1(DEAP1)in rice.The deap1 mutant anthers produced aberrant pollen grains with defective exine formation and a flattened aperture annulus and exhibited slightly delayed tapetum degradation.DEAP1 encodes a plasma membrane-associated member of groupⅢplant FLAs and is specifically and temporally expressed in reproductive cells and the tapetum layer during male development.Gene expression studies revealed reduced transcript accumulation of genes related to exine formation,aperture patterning,and tapetum development in deap1 mutants.Moreover,DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3 s(OsD6PKL3s),homologs of a known Arabidopsis aperture protein,to affect rice pollen aperture development.Our findings suggested that DEAP1 is involved in male reproductive development and may affect exine formation and aperture patterning,thereby providing new insights into the molecular functions of plant FLAs in male fertility. | Dan Zhou Ting Zou Kaixuan Zhang Pingping Xiong Fuxing Zhou Hao Chen Gongwen Li Kaiyou Zheng Yuhao Han Kun Peng Xu Zhang Shangyu Yang Qiming Deng Shiquan Wang Jun Zhu Yueyang Liang Changhui Sun Xiumei Yu Huainian Liu Lingxia Wang Ping Li Shuangcheng Li | 2022 | Journal of Integrative Plant Biology2022，64，7： | 2 |
| 8 | Enhanced energy-storage performance in a flexible film capacitor with coexistence of ferroelectric and polymorphic antiferroelectric domains显示文摘Advances in flexible electronics are driving dielectric capacitors with high energy storage density toward flexibility and miniaturization.In the present work,an all-inorganic thin film dielectric capacitor with the coexistence of ferroelectric(FE)and antiferroelectric(AFE)phases based on Pb_(0.96)La_(0.04)(Zr_(0.95)Ti_(0.05))O_(3)(PLZT)was prepared on a 2D fluorophlogopite mica substrate via a simple one-step process.The flexible capacitor exhibits a high recoverable energy density(U_(rec))of z 44.2 J/cm^(3),a large electric breakdown strength(E BDS)of 3011 kV/cm,excellent frequency stability(500 Hz-20 kHz)and high thermal stability over 30-190℃.It also demonstrates an outstanding bending endurance,which can maintain a high energy storage performance under various bending radii(R=2-10 mm)or 103 repeated bends at 4 mm.The FE phase is stable near the film surface and the interface with the bottom electrode.The AFE phase with multi-domains has incommensurate modulation structures with super-periodicity of 6.5,6.9 and 5.2.It indicates that the PLZT/LNO/F-Mica capacitor has high potential for energy storage application and may provide great opportunities for exploring new energy storage materials. | Xiaokuo Er Peng Chen Jiesen Guo Yuxuan Hou Xiaobo Yu Pingping Liu Yang Bai Qian Zhan | 2022 | Journal of Materiomics2022，8，2： | 1 |
| 9 | Study of Bandgap Characteristics of 2D Magnetoplasma Photonic Crystal by Using M-FDTD Method显示文摘 | Yang Lixia Xie Yingtao Yu Pingping | 2011 | Microwave and Optical Technology Letters2011，53，8： | 1 |
| 10 | Chemical composition and antioxidative and anti-inflammatory properties of ten commercial mung bean samples显示文摘 | Xiaowei Zhang Pingping Shang Fang Qin Qin Zhou Boyan Gao Haiqiu Huang Haisha Yang Haiming Shi Liangli (Lucy) Yu | 2013 | LWT - Food Science and Technology2013，，1： | 1 |
| 11 | Thermoplastic starch modified with polyurethane microparticles:Effects of hydrophilicity of polyurethane prepolymer显示文摘 | Chen Xiaoxia Zhang Yu Zhang Pingping | 2012 | Starch2012，64，4： | 1 |
| 12 | Clinical research progress in Traditional Chinese Medicine in treating wound healing after anal fistula surgery显示文摘In the study of the mechanism of wound healing after anal fistula surgery,how to scientifically and efficiently promote wound healing is of great significance.At present,modern medical treatment of wounds after anal fistula surgery mostly focuses on physical therapy intervention,new wound dressing and packing,and external application of growth factors.However,these therapies have many problems,and there is still no consensus on their clinical use.Traditional Chinese Medicine(TCM)has several methods to promote wound healing,such as oral administration,rubbing,and fumigation,which have a long history and obvious efficacy,but research in this area is relatively scattered and lacks classification and summarizing.Therefore,this paper analyzes and summarizes the existing research on TCM for promotion of wound healing after anal fistula surgery,carries out targeted analyses according to different clinical syndromes and treatment methods,and analyzes the defects in current research and anticipates future research trends in order to provide theoretical support for the advantages of TCM in promoting wound healing after anal fistula surgery. | MEI Pingping FENG Wenzhe SHI Peng ZHANG Wenxiu ZHUANG Yu | 2023 | Journal of Traditional Chinese Medicine2023，43，5： | 1 |
| 13 | The Combination of Mycophenolate Mofetil with Corticosteroids Induces Remission of Henoch-Schonlein Purpura Nephritis显示文摘 | Ren Pingping Han Fei Chen Liangliang Xu Yu Wang Ying Chen Jianghua | 2012 | American Journal of Nephrology2012，，3： | 1 |
| 14 | Research on the Pedestrian Re-Identification Method Based on Local Features and Gait Energy Images显示文摘The appearance of pedestrians can vary greatly from image to image,and different pedestrians may look similar in a given image.Such similarities and variabilities in the appearance and clothing of individuals make the task of pedestrian re-identification very challenging.Here,a pedestrian re-identification method based on the fusion of local features and gait energy image(GEI)features is proposed.In this method,the human body is divided into four regions according to joint points.The color and texture of each region of the human body are extracted as local features,and GEI features of the pedestrian gait are also obtained.These features are then fused with the local and GEI features of the person.Independent distance measure learning using the cross-view quadratic discriminant analysis(XQDA)method is used to obtain the similarity of the metric function of the image pairs,and the final similarity is acquired by weight matching.Evaluation of experimental results by cumulative matching characteristic(CMC)curves reveals that,after fusion of local and GEI features,the pedestrian re-identification effect is improved compared with existing methods and is notably better than the recognition rate of pedestrian re-identification with a single feature. | Xinliang Tang Xing Sun Zhenzhou Wang Pingping Yu Ning Cao Yunfeng Xu | 2020 | Computers, Materials & Continua2020，，8： | 1 |
| 15 | Using 18 F-Fluorodeoxyglucose Positron Emission Tomography to Estimate the Length of Gross Tumor in Patients With Squamous Cell Carcinoma of the Esophagus显示文摘 | Xiaojun Zhong Jinming Yu Baijiang Zhang Dianbin Mu Weidi Zhang Daotang Li Anqin Han Pingping Song Hui Li Guoren Yang Feng-Ming Kong Zheng Fu | 2009 | International Journal of Radiation Oncology Biology Physics2009，，1： | 1 |
| 16 | Experimental investigation of crossflow characteristics in multi-jets system at small nozzle-to-plate spacing显示文摘To further extend knowledge about the detailed knowledge on the crossflow characteristics in a multi-jets system under a confined space,particle image velocimetry (PIV) was employed to investigate the flow structures together with the distributions of the mean velocity components for Reynolds numbers (Re) ranging from 6 213 to 13 418,nozzle-to-plate spacing (H/D) varying from 0. 20 to1. 25,respectively. Results show that the crossflow configuration is significantly different from those of large nozzle-to-plate spacing. In addition,a turning point H/D=0.50 is revealed in the profile of the normalized maximum radial velocity which is associated with the heat transfer distribution on the impingement plate. | ZHU Keqian SUN Tao YU Pingping LI Meixiang YUAN Ningyi DING Jianning | 2019 | 排灌机械工程学报2019，37，2： | 1 |
| 17 | Study ofbandgap characteristics of 2D magnetoplasma photon-ic crystal by using M-FDTD method 显示文摘 | YANG Lixia XIE Yingtao YU Pingping | 2011 | Microwaveand Optical Technology Letters2011，53，8： | 1 |
| 18 | Small-molecule diketopyrrolopyrrole-based therapeutic nanoparticles for photoacoustic imaging-guided photothermal therapy显示文摘治疗学的 nanoparticles (NP ) 基于 donor-acceptor-donor,结构化的小器官的分子 diketopyrrolopyrrole (SDPP ) 用一条简单降水途径被准备。这些在红外线附近的吸收放射的 NP 有高 photothermal 变换效率并且能有选择地通过提高的渗透和保留效果指向癌症纸巾。得益于这些优点, SDPP NP 能用作一个优秀治疗学的代理人为高度有效并且 noninvasive photoacoustic 指导成像的 photothermal 治疗。用老鼠肿瘤模型的实验证明 SDPP NP 在激光照耀下面展出了非凡的肿瘤脱离能力(660 nm, 1.0 W ? 敳獮瑩癩? 慮潮戭潩椠瑮牥慦散漠 ? 桴獩 ?? 楨牥牡档捩污祬猠牴 ' 壮肭?業牣景畬摩捩搠癥捩 ? 眠楨档猠潨敷 ? 楨桧攠晦捩敩据 ? 景 ?? 挠灡畴敲吗? | Yu Cai Weili Si Qianyun Tang Pingping Liang Chengwu Zhang Peng Chen QiZhang Wei Huang Xiaochen Dong | 2017 | Nano Research2017，10，3： | 1 |
| 19 | Increasing stability of antibody via antibody engineering: Stability engineering on an anti‐hVEGF显示文摘 | Shuang Wang Ming Liu Dadi Zeng Weiyi Qiu Pingping Ma Yunzhou Yu Hongyan Chang Zhiwei Sun | 2014 | Proteins2014，，： | 1 |
| 20 | Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method显示文摘Claviceps purpurea produces many pharmacologically important ergot alkaloids(EAS),which are widely used to treat migraine and hypertension and to aid childbirth.Although an EAS biosynthetic cluster of C.purpurea has been discovered more than 20 years ago,the complete biosynthetic pathway of EAS has not been fully characterized until now.The main obstacle to elucidating this pathway and strain modification is the lack of efficient genome-editing tools for C.purpurea.The conventional gene manipulation method for C.purpurea relies on homologous recombination(HR),although the efficiency of HR in C.purpurea is very low(~1-5%).Consequently,the disruption of target genes is laborious and time-consuming.Although CRISPR/Cas9 genome-editing methods based on in vivo Cas9 expression and gRNA transcription have been reported recently,their gene-disruption efficiency is still very low.Here,we developed an efficient genome-editing system in C.purpurea based on in vitro assembled CRISPR/Cas9 gRNA ribonucleoprotein complexes.As proof of principle,three target genes were efficiently knocked out using this CRISPR/Cas9 ribonucleoprotein complex-mediated HR system,with editing efficiencies ranging from 50%to 100%.Inactivation of the three genes,which are closely related to uridine biosynthesis(ura5),hypha morphology(rac),and EAS production(easA),resulted in a uridine auxotrophic mutant,a mutant with a drastically different phenotype in axenic culture,and a mutant that did not produce EAS,respectively.Our ribonucleoprotein-based genome-editing system has a great advantage over conventional and in vivo CRISPR/Cas9 methods for genome editing in C.purpurea,which will greatly facilitate elucidation of the EAS biosynthetic pathway and other future basic and applied research on C.purpurea. | Lu Yu Meili Xiao Zhihua Zhu Yinmei Wang Zhihua Zhou Pingping Wang Gen Zou | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
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