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| 1 | New means to monitor the effect of glucocorticoid therapy in children显示文摘AIM:To study the individual effects of glucocorticoid (GC) therapy on the state ofimmune activation in patient serum.METHODS:We developed a novel assay in which the effect of corticosteroid-treated patient serum on healthy donor peripheral blood mononuclear cells (target cells) was studied,with a panel of markers for effector [interferon (IFN)γ and interleukin (IL)-5] and regulatory T cells (FOXP3 and glucocorticoid-induced tumor necrosis factor receptor,GITR).The study group comprised 19 children with inflammatory bowel disease.The individual effect of patient serum on target cells was analyzed prior to GC therapy and 2 wk later.RESULTS:The effect of GC therapy mediated by patient serum was seen as a decrease in the target cells expression of regulatory T-cell-related markers GITR (median suppression 24%,range of suppression 1%-63%,in 2 cases increase of 6% and 77%,P < 0.01 for mitogen-activated target cells) and FOXP3 (median suppression 33%,range of suppression 0%-79%,in one case an increase of 173%,P < 0.05 for resting cells),and secretion of IFNγ [from a mean of 87 700 pg/mL (SD 33 900 pg/mL) to 60 900 pg/mL (SD 44 200 pg/mL) in mitogen-activated target cells,13 of the cases showed a decrease,P < 0.01].The total or weight-related prednisolone dose did not correlate with the patient-seruminduced changes in the target cell markers.CONCLUSION:GC response could be monitored at an individual level by studying the effect of patient serum on signaling pathways of target immune cells. | Hanne Rintamki Harri M Salo Outi Vaarala Kaija-Leena Kolho | 2010 | World Journal of Gastroenterology2010，16，9： | 4 |
| 2 | Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution显示文摘AIM:To optimize a xeno-free cryopreservation protocol for primary human hepatocytes.METHODS:The demand for cryopreserved hepatocytes is increasing for both clinical and research purposes.Despite several hepatocyte cryopreservation protocols being available,improvements are urgently needed.We first compared controlled rate freezing to polystyrene box freezing and did not find any significant change between the groups.Using the polystyrene box freezing,we compared two xeno-free freezing solutions for freezing of primary human hepatocytes:a new medium(STEM-CELLBANKER,CB),which contains dimethylsulphoxide(DMSO) and anhydrous dextrose,both permeating and non-permeating cryoprotectants,and the frequently used DMSO-University of Wisconsin(DMSOUW) medium.The viability of the hepatocytes was assessed by the trypan blue exclusion method as well as a calcein-esterase based live-dead assay before and after cryopreservation.The function of the hepatocytes was evaluated before and after cryopreservation by assessing enzymatic activity of 6 major cytochrome P450 isoforms(CYPs):CYP1A2,CYP2C9,CYP2C19,CYP2D6,CYP3A4 and CYP3A7.RESULTS:The new cryoprotectant combination preserved hepatocyte viability significantly better than the standard DMSO-UW protocol(P < 0.01).There was no significant difference in viability estimation between both the trypan blue(TB) and the Live-Dead Assay methods.There was a correlation between viability of fresh hepatocytes and the difference in cell viability between CB and DMSO protocols(r 2 = 0.69) using the TB method.However,due to high within-group variability in the activities of the major CYPs,any statistical between-group differences were precluded.Cryopreservation of human hepatocytes using the cryoprotectant combination was a simple and xeno-free procedure yielding better hepatocyte viability.Thus,it may be a better alternative to the standard DMSO-UW protocol.Estimating CYP activities did not seem to be a relevant way to compare hepatocyte function between different groups due to high normal variability between different liver samples.CONCLUSION:The cryoprotectant combination may be a better alternative to the standard DMSO-UW protocol in primary human hepatocyte cryopreservation. | Mohammed Saliem Frida Holm Rosita Bergstrm Tengzelius Carl Jorns Lisa-Mari Nilsson Bo-Gran Ericzon Ewa Ellis Outi Hovatta | 2012 | World Journal of Hepatology2012，4，5： | 2 |
| 3 | An Activity-Based Costing Model for Logistics Operations of Manufacturers and Wholesalers显示文摘 | OUTI M | 2000 | International Journal of Logistics:Research & Applications2000，3，1： | 1 |
| 4 | An Activity-based costing model for logistics operations of manufacturers and wholesalers显示文摘 | Outi M | 2000 | International Journal of Logistics: Research and Applications2000，3，1： | 1 |
| 5 | Bacterial biofilm formation on polyvinyl chloride, polyethylene and stainless steel exposed to ozonated water 显示文摘 | Outi M Zacheus Eila K Iivanainen | 2000 | Wat Res2000，，1： | 1 |
| 6 | Patient-controlled sedation with propofol and remifentanil for ERCP: a randomized, controlled study显示文摘 | Maxim Mazanikov Marianne Udd Leena Kyl?np?? Outi Lindstr?m Pekka Aho Jorma Halttunen Martti F?rkkil? Reino P?yhi? | 2011 | Gastrointestinal Endoscopy2011，，2： | 1 |
| 7 | Pa- tients'perceptions of orthognathic treatment, well-being, and psychologi- cal or psychiatricstatus: a systematic, review 显示文摘 | Alanko Outi M E Svedstrom-Oristo Anna-Liisa Tuomisto Martti T | 2010 | Acta odontologica Scan- dinavica2010，6815，： | 1 |
| 8 | Nordic MCL 2 trial update: six‐year follow‐up after intensive immunochemotherapy for untreated mantle cell lymphoma followed by BEAM or BEAC ?+?autologous stem‐cell support: still very long survival but late relapses do occur显示文摘 | Christian H. Geisler Arne Kolstad Anna Laurell Mats Jerkeman Riikka R?ty Niels S. Andersen Lone B. Pedersen Mikael Eriksson Marie Nordstr?m Eva Kimby Hans Bentzen Outi Kuittinen Grete F. Lauritzsen Herman Nilsson‐Ehle Elisabeth Ralfki?r Mats Ehinger Chris | 2012 | Br J Haematol2012，，3： | 1 |
| 9 | Role of microtubules in the movement of the vegetative nucleus and generative cell in tobacco pollen tubes显示文摘 | Helena ?str?m Outi Sorri Marjatta Raudaskoski | 1995 | Sexual Plant Reproduction1995，，2： | 1 |
| 10 | Preoperative hepatic 3D models:Virtual liver resection using three-dimensional imaging technique显示文摘 | Outi S Heikki M | 2005 | European Journal of Radiology2005，56，： | 1 |
| 11 | Serum immune-activation potency and response to anti-TNF-α therapy in Crohn's disease显示文摘AIM:To study whether immune-activation stage in serum of adult Crohn's disease (CD) patients correlates with disease activity and with treatment response to antitumor necrosis factor-α (TNF-α) therapy.METHODS:Serum samples were obtained from 15 adult CD patients introduced to anti-TNF-α therapy.The individual stage of immune activation was studied applying our new in vitro assay,in which target cells (donor derived peripheral blood mononuclear cells) were cultured with patient serum and the T-cell activation induced by the patient serum was studied using a panel of markers for effector [interferon γ (IFNγ),interleukin (IL)-5] and regulatory T-cells [forkhead transcription factor 3 (FOXP3) and glucocorticoid-induced tumour necrosis factor receptor (GITR)].The endoscopic disease activity was assessed with the Crohn's disease endoscopic index of severity (CDEIS) before and 3 mo after therapy with an anti-TNF-α agent.RESULTS:Low induction of FOXP3 and GITR in target cells cultured in the presence of patient serum was associated with high disease activity i.e.CDEIS assessed before therapy (r=-0.621,P=0.013 and r=-0.625,P=0.013,respectively).FOXP3 expression correlated inversely with pre-treatment erythrocyte sedimentation rate (r=-0.548,P=0.034).Low serum induced FOXP3 (r=-0.600,P=0.018) and GITR (r=-0.589,P=0.021) expression and low IFNγ secretion from target cells (r =-0.538,P=0.039) associated with treatment response detected as a decrease in CDEIS.CONCLUSION:The immune-activation potency in the patient serum prior to anti-TNF-α therapy reflected intestinal inflammation and the therapeutic response. | Hanne Rintamki Taina Sipponen Harri M Salo Outi Vaarala Kaija-Leena Kolho | 2010 | World Journal of Gastroenterology2010，16，46： | 1 |
| 12 | Gluten-dependent Small Bowel Mucosal Transglutaminase 2–specific IgA Deposits in Overt and Mild Enteropathy Coeliac Disease显示文摘 | Outi Koskinen Pekka Collin Ilma Korponay-Szabo Teea Salmi Sari Iltanen Katri Haimila Jukka Partanen Markku M?ki Katri Kaukinen | 2008 | Journal of Pediatric Gastroenterology and Nutrition2008，，4： | 1 |
| 13 | Treatment of Abdominal Compartment Syndrome with Subcutaneous Anterior Abdominal Fasciotomy in Severe Acute Pancreatitis显示文摘 | Ari K. Lepp?niemi MD PhD Piia A. Hienonen MD Jukka E. Siren MD PhD Anne H. Kuitunen MD PhD Outi K. Lindstr?m MD Esko A. J. Kemppainen MD PhD | 2006 | World Journal of Surgery2006，，10： | 1 |
| 14 | Impaired Bone Health in Inflammatory Bowel Disease: A Case–Control Study in 80 Pediatric Patients显示文摘 | Saila Laakso Helena Valta Matti Verkasalo Sanna Toiviainen-Salo Heli Viljakainen Outi M?kitie | 2012 | Calcified Tissue International2012，，2： | 1 |
| 15 | Effect of anaesthesia on contrast-enhanced ultrasound of the feline spleen显示文摘 | Merja R L Marja R R Outi M V | | 0，，： | 1 |
| 16 | DNA Copy Number Changes in Diffuse Large B-Cell Lymphoma-Comparative Genomic Hybridization Study显示文摘 | Outi M Heikki J Kaarle F | 1996 | Blood1996，87，12： | 1 |
| 17 | Germinatio n of Oat and Quinoa and Evaluation of the Malts as Gluten Free Baking Ingredients显示文摘 | Outi E M~kinen Emanuele Zannini Elke K Arendt | 2013 | Plant Foods Human Nutrition2013，68，： | 1 |
| 18 | Depth of invasion, tumor budding, and worst pattern of invasion: Prognostic indicators in early‐stage oral tongue cancer显示文摘 | Alhadi Almangush Ibrahim O. Bello Harri Keski–S?ntti Laura K. M?kinen Joonas H. Kauppila Matti Pukkila Jaana Hagstr?m Jussi Laranne Satu Tommola Outi Nieminen Ylermi Soini Veli‐Matti Kosma Petri Koivunen Reidar Grénman Ilmo Leivo Tuula Salo | 2014 | Head Neck2014，，6： | 1 |
| 19 | An Activity-Based Costing Model for Logistics Operations of Manufacturers and Wholesalers显示文摘 | OUTI M | 2000 | International Journal of Logistics:Research&Applications2000，3，1： | 1 |
| 20 | Preoperative hepatic 3D models: Virtual liver resection using three-dimensional imaging technique显示文摘 | Kirsti Numminen Outi Sipil? Heikki M?kisalo | 2005 | European Journal of Radiology2005，，2： | 1 |
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