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| 1 | Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization显示文摘AIM: To determine the role of interleukin (IL)-17 in gastric ulcerogenesis. METHODS: Thirty-six gastric ulcer (GU) patients and 29 non-ulcer (NU) patients were enrolled in this study. Mucosal biopsy samples were obtained from the gastric antrum and GU site during endoscopy. Samples were used in in situ stimulation for 48 h in the presence of 10 μg/mL phytohemagglutinin-P (PHA), histological examination, and Helicobacter pylori(H pylori) culture. IL-17 and IL-8 protein levels in culture supernatants were assayed by ELISA. IL-17 mRNA expression was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). H pylori cagA and vacA status was assessed by reverse hybridization using a line probe assay (LiPA). IL-8 levels in culture supernatants were assayed after AGS cells were co-cultured with H pylori strain 26 695 or recombinant human (rh) IL-17. RESULTS: All 36 GU patients and 15 of 29 NU patients were found to be H pylori-positive, while 14 NU patients were H pylori-negative. All 51 H pylori strains from both GU and NU patients were cagA- and vacAs1/m1-positive. Antral mucosal tissues from H pylori-positive patients contained significantly (H pylori-positive NU patients: median 467 pg/mg/protein, range 53-2 499; H pylori-negative NU patients: median 104 pg/mg/protein, range 16-312, P<0.0005) higher levels of IL-17 than those from uninfected patients. IL-17 levels at the ulcer site were significantly (ulcer site: median 1356 pg/mg/protein, range 121-1 3730; antrum: median 761 pg/mg/protein, range 24-7 620, P<0.005) higher than those at distant sites in the antrum. Biopsies from H pylori-positive GU and NU patients showed IL-17 mRNA expression in all samples whereas those from the antrum of the H pylori-negative controls showed no detectable expression. A significant correlation was seen between IL-17 and IL-8 levels at each biopsy site (ulcer: r= 0.62, P<0.0001; antrum: r = 0.61, P<0.0001) in GU patients. RhIL-17 and H pylori strain 26 695 each stimulated IL-8 production from AGS cells. CONCLUSION: IL-17 may play an important role in the inflammatory response to H pylori colonization, and may ultimately influence the outcome of H pylori-associated diseases that arise within the context of gastritis. | Tomokazu Mizuno Takafumi Ando Kazuo Nobata Tomoyuki Tsuzuki Osamu Maeda Osamu Watanabe Masaaki Minami Kenji Ina Kazuo Kusugami Richard M. Peek Hidemi Goto | 2005 | World Journal of Gastroenterology2005，11，40： | 18 |
| 2 | Cytomegalovirus infection in severe ulcerative colitis patients undergoing continuous intravenous cyclosporine treatment in Japan显示文摘AIM: To investigate active cytomegalovirus (CMV) infection following the cyclosporine A (CyA) treatment of steroid-refractory ulcerative colitis (UC).METHODS: Twenty-three patients with severe UC not responding to steroid therapy (male 14, and female 9) enrolled at Nagoya University Hospital from 1999 to 2005. They received continuous intravenous infusion of CyA (average 4 mg/kg per day) for 1 mo. Serum and colonic biopsy samples were collected before CyA treatment and 4 d, 10 d, 20 d, and 30 d after treatment. Patients were evaluated for CMV by using serology (IgM antibody by ELISA), quantitative real-time PCR for CMV DNA, and histopathological assessment of hematoxylin and eosin (HE)-stained colonic biopsies. CMV infection was indicated by positive results in any test. RESULTS: No patients had active CMV infection before CyA treatment. Eighteen of 23 UC patients treated with CyA were infected with active CMV (IgM antibody in 16/23 patients, 69.6%; CMV DNA in 18/23 patients, 78.2%; and inclusion bodies in 4/23 patients, 17.3%). There was no difference in the active CMV-infection rate between males and females. Active CMV infection was observed after approximately 8 d of CyA treatment, leading to an exacerbation of colitis. Fifteen of these 18 patients with active CMV infection (83.3%) required surgical treatment because of severe deteriorating colitis. Treatment with ganciclovir rendered surgery avoidable in three patients. CONCLUSION: Our results suggest that active CMVinfection in severe UC patients treated with CyA is associated with poor outcome. Further, ganciclovir is useful for treatment of CMV-associated UC after immuno-suppressive therapy. | Masaaki Minami Michio Ohta Teruko Ohkura Takafumi Ando Naoki Ohmiya Yasumasa Niwa Hidemi Goto | 2007 | World Journal of Gastroenterology2007，13，5： | 13 |
| 3 | Normalization of intracellular Ca^2+ induces a glucose-responsive state in glucose-unresponsive B-cells显示文摘 | Kohtaro Minami Masaaki Yokokum Nobuko Ishizuka | 2002 | J Biol Chem2002，277，25： | 1 |
| 4 | Treatment of Cerebral Vasospasm with Intra-arterial Fasudil Hydrochloride显示文摘 | Kazuhiro Tanaka Hiroaki Minami Masaaki Kota Keiichi Kuwamura Eiji Kohmura | 2005 | Neurosurgery2005，，2： | 1 |
| 5 | Spectrum of Epstein-Barr virus-related diseases: a pictorial review显示文摘 | Eriko Maeda Masaaki Akahane Shigeru Kiryu Nobuyuki Kato Takeharu Yoshikawa Naoto Hayashi Shigeki Aoki Manabu Minami Hiroshi Uozaki Masashi Fukayama Kuni Ohtomo | 2009 | Japanese Journal of Radiology2009，，1： | 1 |
| 6 | Gene deletions in Japanese patients with Duchenne and Becker muscular dystrophy显示文摘 | Jun-ichi Asano Shunji Tomatsu Kazuko Sukegawa Seiji Yamaguchi Yuko Ikedo Ryoji Minami Mitsuo Iida Masaaki Nishimura Masanori Nakagawa Morio Ohshiro Tadao Orii | 1990 | The Japanese Journal of Human Genetics1990，，2： | 1 |
| 7 | Use of a Combination of Brushing Technique and the Loop-Mediated Isothermal Amplification Method as a Novel,Rapid,and Safe System for Detection of Helicobacter pylori显示文摘 | MASAAKI MINAMI MICHIO OHTA TERUKO OHKURA | 2006 | Journal of Clincal Microbiology2006，44，11： | 1 |
| 8 | Five-year follow-up with low-dose tacrolimus in patients with myasthenia gravis显示文摘 | Naoya Minami Naoto Fujiki Shizuki Doi Koji Shima Masaaki Niino Seiji Kikuchi Hidenao Sasaki | 2010 | Journal of the Neurological Sciences2010，，1： | 1 |
| 9 | Chronological changes in the liver after temporary partial portal venous occlusion显示文摘AIM:To investigate time-dependent changes caused by temporal portal vein obstruction and subsequent reperfusion in the lobe with or without an occluded portal vein.METHODS:The portal vein(PV)of the anterior lobe of the liver of a male Wistar rat(8 wk-old)was obstructed(70%)for 12,24,36 and 48 h,respectively,and models were sacrificed at 48 h after reperfusion(each group:n=10).The histological changes and the status of liver regeneration were compared between a liver biopsy performed on each lobe after temporary obstruction of the portal vein in the same rat liver,and the liver extracted at the time of sacrifice(48 h after reperfusion).RESULTS:With regard to the obstructed lobe,the liver weight/body weight ratio significantly decreased according to obstruction time.On the other hand,in thenon-obstructed lobe,there were no significant differences within each group.The duration of PV occlusion did not seem to be strong enough to introduce liver weight increase.Stimulation of liver regeneration was brought about in the non-occluded lobe by 12-h occlusion,and was sustained even at 48 h after reperfusion.The obstructed lobe atrophied with the passage of time in the obstructed state.However,the proliferating-cell nuclear antigen labeling index also increased at 48 h after reperfusion,and a repair mechanism was observed.CONCLUSION:Temporary blood flow obstruction of the portal vein may become a significant trigger for liver regeneration,even with an obstruction of 12 h. | Koji Hamasaki Susumu Eguchi Akihiko Soyama Masaaki Hidaka Mitsuhisa Takatsuki Fumihiko Fujita Kengo Kanetaka Shigeki Minami Tamotsu Kuroki | 2013 | World Journal of Gastroenterology2013，19，34： | 0 |
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