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| 1 | AtDREB1A基因在菊花中的异源表达提高了植株对干旱和盐渍胁迫的耐性显示文摘将携带有AtDREBlA基因,并以35S或rd29A启动子驱动的植物表达载体转入地被菊花(Dendranthema grandiflorum)的粉色品种‘Fall color’.与野生型相比,35S:DREBIA转基因植株表现出严重的生长抑制,而rd29A:DREB1A植株生长正常.RT-PCR检测表明,在胁迫条件下, AtDREB1A基因在35S:DREB1A转基因植株中呈现组成型过量表达,而在rd29A:DREB1A植株中则是受胁迫诱导型过量表达.这两种启动子驱动的转基因植株对干旱和盐溃胁迫都表现出较强的耐性,其中rd29A:DREB1A植株耐性显著强于35S:DREB1A植株.rd29A:DREB1A植株中的脯氨酸含量和SOD活性都强烈地被胁迫诱导升高,且高于35S:DREB1A植株.这些结果表明,在地被菊花中表达AtDREB1A基因可以提高植株对干旱和盐渍胁迫的耐性,同时这些耐性的升高可能与脯氨酸含量和SOD活性的上升有关. | 洪波 仝征 马男 李建科 Mie Kasuga Kazuko Yamaguchi-Shinozaki 高俊平 | 2006 | 中国科学(C辑)2006，36，3： | 25 |
| 2 | 根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化显示文摘以地被菊花(Dendranthema grandiflorum cv.White Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR-Southern检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5~0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。 | 洪波 张常青 李邱华 高俊平 KASUGA Mie SHINOZAKI-YAMAGUCHI Kazuko | 2005 | 农业生物技术学报2005，13，3： | 20 |
| 3 | Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance显示文摘DNA cassette containing an AtDREB1A cDNA and a nos terminator,driven by a cauli- flower mosaic 35S promoter,or a stress-inducible rd29A promoter,was transformed into the ground cover chrysanthemum(Dendranthema grandiflorum)'Fall Color'genome.Compared with wild type plants,severe growth retardation was observed in 35S:DREB1A plants,but not in rd29A:DREB1A plants.RT-PCR analysis revealed that,under stress conditions,the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants,but was over-expressed inductively in rd29A:DREB1A plants.The transgenic plants exhibited tolerance to drought and salt stress,and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants.Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions.These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum,and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. | KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko | 2006 | Science China(Life Sciences)2006，49，5： | 18 |
| 4 | 紫色土集水区氮素收支状况与平衡分析显示文摘通过 1999- 2 0 0 0年间定位观测和模拟实验 ,研究了紫色土丘陵集水区生态系统氮素养分的收入与支出参数及系统氮素平衡。结果表明 ,本区农田氮素略有盈余 ,平均为 4 2kg hm2 ,其中氮肥施用量较大 ,平均达到 35 8kg hm2 ;而林地在系统氮素循环和平衡中发挥了重要作用 ,是系统氮素从亏缺转变为盈余驱动因素之一 ;系统中有机厩肥和化肥是主要的氮素来源 ,但损失严重 ,利用率不高 ,应注意由此引起的对大气和水体的农业非点源污染问题。 | 彭奎 朱波 Kazuko Abe 游翔 | 2001 | 山地学报2001，19，S1： | 9 |
| 5 | Differential effects of physical activity and sleep duration on cognitive function in young adults显示文摘Purpose: Although exercise and sleep duration habits are associated with cognitive function, their beneficial effects on cognitive function remain unclear. We aimed to examine the effect of sleep duration and daily physical activity on cognitive function, elucidating the neural mechanisms using near-infrared spectroscopy(NIRS).Methods: A total of 23 healthy young adults(age 22.0 ± 2.2 years) participated in this study. Exercise amount was assessed using a uniaxial accelerometer. We evaluated total sleep time(TST) and sleep efficiency by actigraphy. Cognitive function was tested using the N-back task, the Wisconsin Card Sorting Test(WCST), and the Continuous Performance Test—Identical Pairs(CPT-IP), and the cortical oxygenated hemoglobin levels during a word fluency task were measured with NIRS.Results: Exercise amount was significantly correlated with reaction time on 0- and 1-back tasks(r = —0.602, p = 0.002; r = —0.446, p = 0.033,respectively), whereas TST was significantly correlated with % corrects on the 2-back task(r = 0.486, p = 0.019). Multiple regression analysis,including exercise amount, TST, and sleep efficiency, revealed that exercise amount was the most significant factor for reaction time on 0- and 1-back tasks(b = —0.634, p = 0.002; b = —0.454, p = 0.031, respectively), and TST was the most significant factor for % corrects on the 2-back task(b = 0.542, p = 0.014). The parameter measured by WCST and CPT-IP was not significantly correlated with TST or exercise amount. Exercise amount, but not TST, was significantly correlated with the mean area under the NIRS curve in the prefrontal area(r = 0.492, p = 0.017).Conclusion: Exercise amount and TST had differential effects on working memory and cortical activation in the prefrontal area. Daily physical activity and appropriate sleep duration may play an important role in working memory. | Kazuko Kato Kunihiro Iwamoto Naoko Kawano Yukihiro Noda Norio Ozaki Akiko Noda | 2018 | Journal of Sport and Health Science2018，7，2： | 5 |
| 6 | HsfAld, a Protein Identified via FOX Hunting Using Thellungiella salsuginea cDNAs Improves Heat Tolerance by Regulating Heat-Stress-Responsive Gene Expression显示文摘Thellungiella salsuginea (先前 T。halophila ) ,种仔细与 Arabidopsis (Arabidopsis thaliana ) 有关,是容忍的不仅高腌层次,而且到寒心,结冰,并且臭氧。这里,我们报导那 T。salsuginea 也比 Arabidopsis 显示出更大的热忍耐。我们识别了 T。作为基因,那能授与的 salsuginea HsfA1d (TsHsfA1d ) 在 Arabidopsis 上标记热忍耐。TsHsfA1d 经由全身的 cDNA 被识别从许多 heat-stress-related T 之中狩猎的过去表示的基因(狐狸) 。salsuginea cDNAs。转基因的 Arabidopsis overexpressing TsHsfA1d 在正常生长温度下面在 Arabidopsis AtHsfA1 regulon 显示出许多基因的组成的起来规定。在 Arabidopsis 叶肉原物, TsHsfA1d 在原子核和细胞质是局部性的。TsHsfA1d 也与 AtHSP90 交往了,它否定地由在细胞质形成 HsfA1HSP90 建筑群调整 AtHsfA1s。TsHsfA1d 的部分原子本地化在正常温度在转基因的植物导致了 AtHsfA1d regulon 的表示,是可能的。我们也发现转基因的 Arabidopsis 植物 overexpressing AtHsfA1d 比野类型的植物更热容忍、起来调整 HsfA1d regulon 的表示在 TsHsfA1d-overexpressing 植物被观察。我们建议 TsHsfA1d 和 AtHsfA1d 的产品作为 Arabidopsis 热压力反应的积极管理者工作并且将为在另外的植物的热压力忍耐的改进有用。 | Yukari Higashi Naohiko Ohama Tomoko Ishikawa Taku Katori Ayaka Shimura Kazuya Kusakabe Kazuko Yamaguchi-Shinozaki Junko Ishida Maho Tanaka Motoaki Seki Kazuo Shinozaki Yoichi Sakata Takahisa Hayashi Teruaki Taji | 2013 | Molecular Plant2013，6，2： | 5 |
| 7 | Impairment of IFN-a production capacity in patients with hepatitis C virus and the risk of the development of hepatocellular carcinoma显示文摘AIM: To determine the utility of interferon (IFN) -αproduction capacity in patients with hepatitis C virus (HCV) infection for the measurement of immunosurveillance potential and for the early detection of hepatocellular carcinoma (HCC) by investigating the Sendai virus (HVJ) stimulated IFN-α production capacity of patients with HCV infection.METHODS: HVJ stimulated IFN-α production was determined in a large number of patients with HCV infection and the development of HCC was monitored for 3 years in patients with liver cirrhosis (LC).RESULTS: IFN-α production capacity decreases gradually with the progression of liver disease from chronic hepatitis (CH) to HCC. A significant correlation between the duration of HCV infection and impaired IFN-α production capacity was observed. IFN-α production in patients who developed HCC within 3 years was significantly lower than that of patients who remained in LC without developing HCC.CONCLUSION: Measurement of IFN-α production in LC patients may be useful for the early detection of HCC. | Kazuko Uno Yoshiki Suginoshita Kazuhiro Kakimi Fuminori Moriyasu Mayumi Hirosaki Taro Shirakawa Tsunataro Kishida | 2005 | World Journal of Gastroenterology2005，11，46： | 3 |
| 8 | 多育赛多孢菌性鼻窦炎1例——致病菌的鉴定和体外抗真菌药物敏感性研究显示文摘目的报告国内首例多育赛多孢菌致鼻窦炎,并探讨致病菌的鉴定及其对抗真菌药物体外敏感性。方法取患者左侧上颌窦分泌物进行真菌培养和形态学鉴定,分离菌株β-球蛋白、rDNAITS序列分析确切鉴定,对分离菌进行7种抗真菌药体外药敏试验。结果根据菌株的形态学特点和基因序列结果鉴定为多育赛多孢菌。体外药敏试验显示该菌对7种抗真菌药物耐药。结论多育赛多孢菌所致的真菌病较少见,其确切鉴定靠形态学特征和基因分析。该菌株对多种抗真菌药物耐药。 | 帕丽达.阿布利孜 Takashi Yaguchi 罗德梅 惠艳 Katsuhiro Kamei Kazuko Nishimura | 2010 | 中国真菌学杂志2010，5，1： | 3 |
| 9 | DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression显示文摘 | Yoh Sakuma Qiang Liu Joseph G. Dubouzet Hiroshi Abe Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki | 2002 | Biochemical and Biophysical Research Communications2002，，3： | 3 |
| 10 | DIFFERENTIAL CYTOTOXIC SENSITIVITY AMONG MTT,NR AND ALPASE ACTIVITY ASSAYS IN HUMAN PULP CELLS EXPOSED TO DENTAL RESIN MONOMERS显示文摘A transformed human dental pulp cell(LSC) was made to assess thecytotoxicity of dental resin monomers with MTT assay,NR assay and ALPase activity assay. L- 929 cells were used as comparative cell line. The sensitivity amongMTT,NR and ALPase activity assays and between two types of cells were investigated. The relationship of ALPase activity to two colorimetric assays was also evaluated. According to IC50 and statistics analysis on cytotoxicity curve,there were significance differences between MTT and NR assay or both cell lines for TECDMAmonomer (P<0.005). MTT assay showed lower sensitivity than NR assay in twokinds of cell for both monomer,and LSC cells presented less sensitivity than L-929cells for TEGDMA monomer. The ALPase activity assay revealed the highest sensitivity among the three tested assays. There was a positive relationship between thecontent of ALPase activity and cytotoxic response of the other two assays. The results indicated that the sensitive degree of test assay or cell line may differ from thetest chemicals. MTT assay may be more suitable for screening a wide large of unknown tested materials due to its ralative less viariation with cell lines and moderatesensitivity. | Jiao Sun(Shanghai Biomaterials Research & Test CenterNinth People'Hospital,Shanghai Second Medical University,Shanghai 200023,China)Michiyo TAKAKUWA Kazuko SATO and Atsushige SATO(Dept. of Biomaterials Science,Faculty of DentistryTokyo Medical and Denta | 1998 | Chinese Journal of Biomedical Engineering(English Edition)1998，7，1： | 3 |
| 11 | Systemic hemodynamics in advanced cirrhosis: Concerns during perioperative period of liver transplantation显示文摘Advanced liver cirrhosis is usually accompanied by portal hypertension. Long-term portal hypertension results in various vascular alterations. The systemic hemodynamic state in patients with cirrhosis is termed a hyperdynamic state. This peculiar hemodynamic state is characterized by an expanded blood volume, high cardiac output, and low total peripheral resistance. Vascular alterations do not disappear even long after liver transplantation(LT), and recipients with cirrhosis exhibit a persistent systemic hyperdynamic state even after LT. Stability of optimal systemic hemodynamics is indispensable for adequate portal venous flow(PVF) and successful LT, and reliable parameters for optimal systemic hemodynamics and adequate PVF are required. Even a subtle disorder in systemic hemodynamics is precisely indicated by the balance between cardiac output and blood volume. The indocyanine green(ICG) kinetics reflect the patient's functional hepatocytes and effective PVF, and PVF is a major determinant of the ICG elimination constant(kICG) in the well-preserved allograft. The kICG value is useful to set the optimal PVF during living-donor LT and to evaluate adequate PVF after LT. Perioperative management has a large influence on the postoperative course and outcome; therefore, key points and unexpected pitfalls for intensive management are herein summarized. Transplant physicians should fully understand the peculiar systemic hemodynamic behavior in LT recipients with cirrhosis and recognize the critical importance of PVF after LT. | Tomohide Hori Yasuhiro Ogura Yasuharu Onishi Hideya Kamei Nobuhiko Kurata Motoshi Kainuma Hideo Takahashi Shogo Suzuki Takashi Ichikawa Shoko Mizuno Tadashi Aoyama Yuki Ishida Takahiro Hirai Tomoko Hayashi Kazuko Hasegawa Hiromu Takeichi Atsunobu Ota Yasuhiro Kodera Hiroyuki Sugimoto Taku Iida Shintaro Yagi Kentaro Taniguchi Shinji Uemoto | 2016 | World Journal of Hepatology2016，8，25： | 3 |
| 12 | Organization of cis -acting regulatory elements in osmotic- and cold-stress-responsive promoters显示文摘 | Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki | 2004 | Trends in Plant Science2004，，2： | 2 |
| 13 | Screening of agonistic activities against four nuclear receptors in wastewater treatment plants in Japan using a yeast two-hybrid assay显示文摘To assess the potential endocrine disruptive effects through multiple nuclear receptors(NRs),especially non-steroidal NRs,in municipal wastewater,we examined the agonistic activities on four NRs(estrogen receptor α,thyroid hormone receptor α,retinoic acid receptor α and retinoid X receptor α) of untreated and treated wastewater from municipal wastewater treatment plants(WWTPs) in Japan using a yeast two-hybrid assay.Investigation of the influent and effluent of seven WWTPs revealed that agonistic activities against steroidal and non-steroidal NRs were always detected in the influents and partially remained in the effuents.Further investigation of four WWTPs employing conventional activated sludge,pseudo-anoxic-oxic,anoxic-oxic and anaerobic-anoxic-oxic processes revealed that the ability to reduce the agonistic activity against each of the four NRs varies depending on the treatment process.These results indicated that municipal wastewater in Japan commonly contains endocrine disrupting chemicals that exert agonistic activities on steroidal and non-steroidal NRs,and that some of these chemicals are released into the natural aquatic environment.Although the results obtained in yeast assays suggested that measured levels of non-steroidal NR agonists in the effluent of WWTPs were not likely to cause any biological effect,further study is required to assess their possible risks in detail. | Daisuke Inoue Koki Nakama Kazuko Sawada Taro Watanabe Hisae Matsui Kazunari Sei Tsuyoshi Nakanishi Michihiko Ike | 2011 | Journal of Environmental Sciences2011，23，1： | 2 |
| 14 | Protective effect of urinary trypsin inhibitor on myocardial mitochondria during hemorrhagic shock and reperfusion显示文摘 | Takashi Masuda Kiyotaka Sato Chiharu Noda Kazuko M. Ikeda Atsuhiko Matsunaga Misao N. Ogura Kazuhiko Shimizu Hiroshi Nagasawa Narihisa Matsuyama Tohru Izumi | 2003 | Critical Care Medicine2003，，7： | 2 |
| 15 | Antibody to eosinophil cationic protein suppresses dextran sulfate sodium-induced colitis in rats显示文摘AIM: To produce an antibody against rat eosinophil cationic protein (ECP) and to examine the effects of the antibody in rats with dextran sulfate sodium (DSS)-induced colitis.METHODS: An antibody was raised against rat ECP. Rats were treated with 3% DSS in drinking water for 7 d and received the antibody or normal serum. The colons were exarmined histologically and correlated with clinical symptoms.Immunohistochemistry and Western blot analysis were estimated as a grade of inflammation.RESULTS: The ECP antibody stained the activated eosinophils around the injured crypts in the colonic mucosa.Antibody treatment reduced the severity of colonic ulceration and acute clinical symptoms (diarrhea and/or blood-stained stool). Body weight gain was significantly greater and the colon length was significantly longer in anti-ECP-treated rats than in normal serum-treated rats. Expression of ECP in activated eosinophils was associated with the presence of erosions and inflammation. The number of Ki-67-positive cells in the regenerated surface epithelium increased in anti-ECP-treated rats compared with normal serum-treated rats. Western blot analysis revealed reduced expression of macrophage migration inhibitory factor (MIF) in anti-ECP-treated rats.CONCLUSION: Our results indicate that treatment with ECP antibody, improved DSS-induced colitis in rats, possibly by increasing the regenerative activity of the colonic epithelium and downregulation of the immune response,and suggest that anti-ECP may promote intestinal wound healing in patients with ulcerative colitis (UC). | Kazuko Shichijo Kazuya Makiyama Chun-Yang Wen Mutsumi Matsuu Toshiyuki Nakayama Masahiro Nakashima Makoto Ihara Ichiro Sekine | 2005 | World Journal of Gastroenterology2005，11，29： | 2 |
| 16 | Scheduled infliximab monotherapy to prevent recurrence of Crohn’s disease following ileocolic or ileal resection: A 3‐year prospective randomized open trial显示文摘 | Koji Yoshida Ken Fukunaga Hiroki Ikeuchi Koji Kamikozuru Nobuyuki Hida Yoshio Ohda Yoko Yokoyama Masaki Iimuro Naohisa Takeda Kyoichi Kato Risa Kikuyama Kazuko Nagase Kazutoshi Hori Shiro Nakamura Hiroto Miwa Takayuki Matsumoto | 2011 | Inflamm Bowel Dis2011，，9： | 2 |
| 17 | Chronic Infections and Atherosclerosis显示文摘 | Kiyoshi Ayada Kenji Yokota Kazuko Kobayashi Yehuda Shoenfeld Eiji Matsuura Keiji Oguma | 2009 | Clinical Reviews in Allergy & Immunology2009，，1： | 2 |
| 18 | Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes显示文摘 | Satoko Matsukura Junya Mizoi Takumi Yoshida Daisuke Todaka Yusuke Ito Kyonoshin Maruyama Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki | 2010 | Molecular Genetics and Genomics2010，，2： | 1 |
| 19 | Thionine and ferric chelate compounds as coupled mediators in microbial fuel cells 显示文摘 | Kazuko Tanaka Carmen A Vega Reita Tamamushi | 1983 | Bioelectrochemistry and Bioenergetics1983，11，： | 1 |
| 20 | Palla-dium extraction with N, N, N,1 N*-tetra-n-octyl-thiodigly-colamide显示文摘 | Hirokazu Narita Mikiya Tanaka Kazuko Morisaku | 2008 | Miner Eng2008，21，： | 1 |
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