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| 1 | Antitumor activities of human autologous cytokineinduced killer(CIK)cells against hepatocellular carcinoma cells in vitro and in vivo显示文摘AIM: To characterize the anticancer function of cytokine-induced killer cells (CIK) and develop an adoptiveimmunotherapy for the patients with primary hepatocellularcarcinoma (HCC), we evaluated the proliferation rate,phenotype and the antitumor activity of human CIK cellsfrom healthy donors and HCC patients in vitro and in vivo.METHODS: Peripheral blood mononuclear cells (PBMC) fronhealthy donors and patients with primary HCC were incubatedin vitro and induced into ClK cells in the presence of variouscytokines such as interferon-gamma (IFN-γ), interleukin-1(IL-1), IL-2, and monoclonal antibody (mAb) against CD3.The phenotype and characterization of CIK cells wereidentified by flow cytometric analysis. The cytotoxicity of CIKcells was determined by 51 Cr release assay.RESULTS: The CIK cells were shown to be a heterogeneouspopulation with different cellular phenotypes. Thepercentage of CD3+/CD56+ positive cells, the dominanteffector cells, in total CIK cells from healthy donors andHCC patients, significantly increased from 0.1-0.13 % at day0 to 19.0-20.5 % at day 21 incubation, which suggested thatthe CD3+ CD56+ positive cells proliferated faster than othercell populations of CIK cells in the protocol used in thisstudy. After 28 day in vitro incubation, the ClK cells frompatients with HCC and healthy donors increased by morethan 300-fold and 500-fold in proliferation cell number,respectively. CIK cells originated from HCC patientspossessed a higher in vitro antitumor cytotoxic activity onautologous HCC cells than the autologous lymphokine-activated killer (LAK) cells and PBMC cells. In in vivoanimal experiment, CIK cells had stronger effects on theinhibition of tumor growth in Balb/c nude mice bearing BEL-7402-producing tumor than LAK cells (mean inhibitory rate,84.7 % vs 52.8 %, P < 0.05) or PBMC (mean inhibitoryrate, 84.7% vs37.1%, P<0.01).CONCLUSION: Autologous CIK cells are of highly efficientcytotoxic effector cells against primary hepatocellularcarcinoma cells and might serve as an alternative adoptivetherapeutic strategy for HCC patients. | Fu-Sheng Wang Ming-Xu Liu Bing Zhang Ming Shi Zhou-Yun Lei Wen-Bing Sun Qing-You Du Ju-Mei Chen,Division of Biological Engineering,Beijing Institute of Infectious Diseases,Beijing 100039,China Wen-Bing Sun,Department of Surgery,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2002 | World Journal of Gastroenterology2002，8，3： | 108 |
| 2 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 3 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 4 | Epidemiology of fungal infections in China显示文摘与 immunocompromised 主人的增加的数字,真菌的感染的流行病学的特征经历了世界范围的庞大的变化,在中国包括。在这份报纸,我们越过中国在真菌病上考察了存在数据总结可得到的流行病学的侧面。我们发现在中国的表面的真菌的感染的一般发生是稳定的,但是癣 capitis 的发生减少了,传播线路变化了。由对比,侵略真菌的感染的全面发生继续升起了。candidemia 的出现由除 C 以外的 Candida 种类引起了。albicans 和不平常的 Candida 种类最近在中国增加了的包括的一些。曲霉属菌引起的感染也在最近的年里宣传了,特别地与 azole 抵抗的曲霉属菌 fumigatus 的出现。cryptococcosis 的一个增加的趋势在中国被注意了,与 Cryptococcus neoformans var。grubii 圣 5 遗传型作为占优势的病原体孤立。回顾的研究建议了在中国的 Pneumocystis 肺病的流行病学的特征可能类似于在另外的发展中的国家的那些。地方性的真菌的感染例如在东北中国的 sporotrichosis,必须引起研究,诊断,并且处理警戒。当前,在中国的真菌病上的流行病学的数据可变、碎片。因此,关于在中国的真菌的感染的全国流行病学的研究是对改进国家健康的重要需要。 | Min Chen Yuan Xu Nan Hong Yali Yang Wenzhi Lei Lin Du Jingjun Zhao Xia Lei Lin Xiong Langqi Cai Hui Xu Weihua Pan Wanqing Liao | 2018 | Frontiers of Medicine2018，12，1： | 49 |
| 5 | Characterization of the receptor-binding domain(RBD)of 2019 novel coronavirus:implication for development of RBD protein as a viral attachment inhibitor and vaccine显示文摘The outbreak of Coronavirus Disease 2019(COVID-19)has posed a serious threat to global public health,calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),also known as 2019 novel coronavirus(2019-nCoV).The CoV spike(S)protein plays the most important roles in viral attachment,fusion and entry,and serves as a target for development of antibodies,entry inhibitors and vaccines.Here,we identified the receptor-binding domain(RBD)in SARS-CoV-2 S protein and found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2(ACE2)receptors.SARS-CoV-2 RBD exhibited significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD and could block the binding and,hence,attachment of SARS-CoV-2 RBD and SARS-CoV RBD to ACE2-expressing cells,thus inhibiting their infection to host cells.SARS-CoV RBD-specific antibodies could crossreact with SARS-CoV-2 RBD protein,and SARS-CoV RBD-induced antisera could cross-neutralize SARS-CoV-2,suggesting the potential to develop SARS-CoV RBD-based vaccines for prevention of SARS-CoV-2 and SARS-CoV infection. | Wanbo Tai Lei He Xiujuan Zhang Jing Pu Denis Voronin Shibo Jiang Yusen Zhou Lanying Du | 2020 | Cellular & Molecular Immunology2020，17，6： | 35 |
| 6 | Sleep and Cognitive Abnormalities in Acute Minor Thalamic Infarction显示文摘In order to characterize sleep and the cognitive patterns in patients with acute minor thalamic infarction(AMTI), we enrolled 27 patients with AMTI and 12 matched healthy individuals. Questionnaires about sleep and cognition as well as polysomnography(PSG) were performed on days 14 and 90 post-stroke. Compared to healthy controls, in patients with AMTI, hyposomnia was more prevalent; sleep architecture was disrupted as indicated by decreased sleep efficiency, increased sleep latency, and decreased non-rapid eye movement sleep stages 2 and 3;more sleep-related breathing disorders occurred; and cognitive functions were worse, especially memory. While sleep apnea and long-delay memory recovered to a large extent in the patients, other sleep and cognitive function deficit often persisted. Patients with AMTI are at an increased risk for hyposomnia, sleep structure disturbance,sleep apnea, and memory deficits. Although these abnormalities improved over time, the slow and incomplete improvement suggest that early management should be considered in these patients. | Wei Wu Linyang Cui Ying Fu Qianqian Tian Lei Liu Xuan Zhang Ning Du Ying Chen Zhijun Qiu Yijun Song Fu-Dong Shi Rong Xue | 2016 | Neuroscience Bulletin2016，32，4： | 28 |
| 7 | Breast cancer development and progression:Risk factors,cancer stem cells,signaling pathways,genomics,and molecular pathogenesis显示文摘As the most commonly occurring cancer in women worldwide,breast cancer poses a formidable public health challenge on a global scale.Breast cancer consists of a group of biologically and molecularly heterogeneous diseases originated from the breast.While the risk factors associated with this cancer varies with respect to other cancers,genetic predisposition,most notably mutations in BRCA1 or BRCA2 gene,is an important causative factor for this malignancy.Breast cancers can begin in different areas of the breast,such as the ducts,the lobules,or the tissue in between.Within the large group of diverse breast carcinomas,there are various denoted types of breast cancer based on their invasiveness relative to the primary tumor sites.It is important to distinguish between the various subtypes because they have different prognoses and treatment implications.As there are remarkable parallels between normal development and breast cancer progression at the molecular level,it has been postulated that breast cancer may be derived from mammary cancer stem cells.Normal breast development and mammary stem cells are regulated by several signaling pathways,such as estrogen receptors(ERs),HER2,and Wnt/b-catenin signaling pathways,which control stem cell proliferation,cell death,cell differentiation,and cell motility.Furthermore,emerging evidence indicates that epigenetic regulations and noncoding RNAs may play important roles in breast cancer development and may contribute to the heterogeneity and metastatic aspects of breast cancer,especially for triple-negative breast cancer.This review provides a comprehensive survey of the molecular,cellular and genetic aspects of breast cancer. | Yixiao Feng Mia Spezia Shifeng Huang Chengfu Yuan Zongyue Zeng Linghuan Zhang Xiaojuan Ji Wei Liu Bo Huang Wenping Luo Bo Liu Yan Lei Scott Du Akhila Vuppalapati Hue H.Luu Rex C.Haydon Tong-Chuan He Guosheng Ren | 2018 | Genes & Diseases2018，5，2： | 24 |
| 8 | Pattern and timing of late Cenozoic rapid exhumation and uplift of the Helan Mountain,China显示文摘The Helan Mountain, an intraplate deformation belt in the North China Craton, is located in the northern portion of the China North-South seismic belt, and at the northwestern margin of the Ordos Block. The Cenozoic deformation history of the Helan Mountain is characterized by extension along the eastern Helan Mountain fault (EHSF), resulting in the exhumation and uplift of the Helan Mountain, relative to the rifting of the adjacent Yinchuan Basin. Here we present new apatite fission track (AFT) data from several transects adjacent to the EHSF in the central and northern Helan Mountain. AFT ages from the northern Helan Mountain (Dawukou and Zhengyiguan transects) range from 10 Ma to 89 Ma, whereas AFT ages from the southern Helan Mountain (Suyukou transect) are greater than 71 Ma. The AFT data analysis reveals initiation of rapid uplift and exhumation of the Helan Mountain at 10–12 Ma. Additionally, a plot of the AFT ages versus their mean track length shows a distinctive 'boomerang' pattern indicating that the Helan Mountain experienced a discrete phase of accelerated exhumation beginning at 10-12 Ma. Spatially, AFT samples systematically increase in age away from the EHSF and are consistent with late Cenozoic exhumation that was slow in the southwestern Helan Mountain and rapid in the northeastern Helan Mountain, as well more rapid adjacent to the EHSF and slower away from the EHSF. Obviously, the spatial distribution of late Cenozoic exhumation indicates that normal faulting of the EHSF is related to southwestward tilting and rapid exhumation of the Helan Mountain beginning at 10–12 Ma. The uplift and exhumation of the Helan Mountain was a response to the intensive extension of the northwestern margin of the Ordos Block in the late Cenozoic; this occurred under a regional extensional stress field oriented NW-SE along the Yinchuan-Jilantai-Hetao and the Weihe-Shanxi graben systems adjacent to the Ordos Block. | LIU JianHui ZHANG PeiZhen ZHENG DeWen WAN JinLin WANG WeiTao DU Peng LEI QiYun | 2010 | Science China Earth Sciences2010，53，3： | 23 |
| 9 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 10 | Genome-scale profiling of circulating cell-free DNA signatures for early detection of hepatocellular carcinoma in cirrhotic patients显示文摘Dear Editor,Hepatocellular carcinoma(HCC)is the second most deadly cancer worldwide.1 Cirrhosis of different causes predisposes patients to HCC,increasing the annual HCC incidence by 2%–4%.1 The development of cirrhosis facilitates a series of genetic or epigenetic changes,resulting in the formation of dysplastic nodules,a premalignant stage in HCC.1 HCC diagnosis at an early stage contributes to an improved prognosis with the possibility of curative treatment.Due to the low accuracy of current diagnostic methods,it is urgently needed to explore new non-invasive strategies for early HCC diagnosis in cirrhotic patients. | Lei Chen Ghassan K.Abou-Alfa Bo Zheng Jing-Feng Liu Jian Bai Lu-Tao Du Yun-Song Qian Rong Fan Xiao-Long Liu Lin Wu Jin-Lin Hou Hong-Yang Wang The PreCar Team | 2021 | Cell Research2021，31，5： | 20 |
| 11 | Plasma miR-216a as a potential marker of pancreatic injury in a rat model of acute pancreatitis显示文摘AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury;nonlethal sepsis induced in rats by single puncture cecal ligation and puncture(CLP)was used as the other model to evaluate the specificity of plasma miR-216a compared with twocommonly used markers(amylase and lipase)for acute pancreatitis.Plasmas were sampled from rats at indicated time points and total RNA was isolated.Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS:In the acute pancreatitis model,among five time points at which plasmas were sampled,miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation(compared with 0 h time point,P<0.01,Kruskal-Wallis Test).In the CLP model,plasma amylase and lipase,two commonly used biomarkers for acute pancreatitis,were significantly elevated 24 h after operation(compared with 0 h time point,P<0.01 and 0.05 respectively,Pairwise Bonferroni corrected ttests),while miR-216a remained undetectable among four tested time points.CONCLUSION:Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity. | Xiang-Yu Kong,Yi-Qi Du,Lei Li,Xiao-Hua Man,Yan-Fang Gong,Li-Ning Xiao,Yong-Zhi Zheng,Shang-Xin Deng,Jun-Jun Gu,Zhao-Shen Li,Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China Jian-Qiang Liu,Department of Gastroenterology,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,Fujian Province,China Guo-Kun Wang,Jia-Qi Zhu,Department of Cardiology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China | 2010 | World Journal of Gastroenterology2010，16，36： | 19 |
| 12 | p53 upregulated by HIF-la promotes hypoxiainduced G2/M arrest and renal fibrosis in vitro and in vivo显示文摘Hypoxia plays an important role in the genesis and progression of renal fibrosis.The underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis. | Limin Liu Peng Zhang Ming Bai Lijie He Lei Zhang Ting Liu Zhen Yang Menglu Duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun | 2019 | Journal of Molecular Cell Biology2019，11，5： | 18 |
| 13 | A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30显示文摘Mitochondrial 熔化是为正常 mitochondrial 功能和 mitochondrial DNA 继承混合并且统一 mitochondrial 分隔空间的一个高度协调的过程。Dysregulated mitochondrial 熔化原因 mitochondrial 破碎,反常 mitochondrial 生理学和继承,并且有原因地与很多 neuronal 疾病被连接了。这里,我们识别了 potently 导致了 mitochondrial 熔化在在 Mfn1 或 Mfn2 是缺乏的房间恢复 mitochondrial 网络和氧化呼吸的 diterpenoid 衍生物 15-oxospiramilactone (S3 ) 。线粒体局部性的 deubiquitinase USP30 是 S3 的一个目标。由 S3 的 USP30 的抑制导致 Mfn1/2 的 non-degradative ubiquitination 的增加,它提高 Mfn1 和 Mfn2 活动并且支持 mitochondrial 熔化。通过 USP30 的一个禁止者的使用,因此,我们的学习揭开在支持 mitochondrial 的 Mfns 的 non-degradative ubiquitination 的异乎寻常的功能熔化。 | Wen Yue Ziheng Chen HaiYang Li Chen Yan Ming Chen Du Feng Chaojun Yan Hao Wu Lei Du Yueying Wang Jinhua Liu Xiaohu Huang Laixin Xia Lei Liu Xiaohui Wang Haijing Jin Jun Wang Zhiyin Song Xiaojiang Hao Quan Chen | 2014 | Cell Research2014，24，4： | 18 |
| 14 | Dextral strike-slip of Sanguankou-Niushoushan fault zone and extension of arc tectonic belt in the northeastern margin of the Tibet Plateau显示文摘Sanguankou-Niushoushan 差错(SGK-NSSF ) 的运动学的特征具有到弧的延期的理解的大意义在西藏高原的东北边缘的构造的带。用地调查和各种各样的数据收集方法,包括大规模地质的印射,典型地形学的测量,并且沉积阶层标明日期, SGK-NSSF 展出明显的右罢工滑倒特征并且因此不是一个左首的罢工滑倒差错,由以前的研究人员相信了,这被决定。这研究的结果证明地质的边界为古生代,中生代,和新生代时代都被差错右地打乱,与是类似的指责的排水量。在地志的效果的消除以后,差错的最大的罢工滑倒排水量被发现是 | LEI QiYun ZHANG PeiZhen ZHENG WenJun CHAI ChiZhang WANG WeiTao DU Peng YU JingXing | 2016 | Science China Earth Sciences2016，59，5： | 17 |
| 15 | Submucosal tunneling endoscopic resection: An effective and safe therapy for upper gastrointestinal submucosal tumors originating from the muscularis propria layer显示文摘AIM To evaluate the effectiveness and safety of submucosal tunneling endoscopic resection(STER) and compare its outcomes in esophageal and cardial submucosal tumors(SMTs) of the muscularis propria(MP) layer.METHODS From May 2012 to November 2017, 173 consecutive patients with upper gastrointestinal(GI) SMTs of the MP layer underwent STER. Overall, 165 patients were included, and 8 were excluded. The baseline characteristics of the patients and SMTs were recorded. The en bloc resection rate, complete resection rate,residual rate, and recurrence rate were calculated to evaluate the effectiveness of STER, and the complication rate was recorded to evaluate its safety. Effectiveness and safety outcomes were compared between esophageal and cardial SMTs.RESULTS One hundred and twelve men and 53 women with a mean age of 46.9 ± 10.8 years were included. The mean tumor size was 22.6 ± 13.6 mm. Eleven SMTs were located in the upper esophagus(6.7%), 49 in the middle esophagus(29.7%), 46 in the lower esophagus(27.9%), and 59 in the cardia(35.7%). Irregular lesions accounted for 48.5% of all lesions. STER achieved an en bloc resection rate of78.7%(128/165) for GI SMTs with an overall complication rate of 21.2%(35/165).All complications resolved without intervention or were treated conservatively without the need for surgery. The en bloc resection rates of esophageal and cardial SMTs were 81.1%(86/106) and 72.1%(42/59), respectively(P = 0.142), and the complication rates were 19.8%(21/106) and 23.7%(14/59), respectively,(P =0.555). The most common complications for esophageal SMTs were gas-related complications and fever, while mucosal injury was the most common for cardial SMTs.CONCLUSION STER is an effective and safe therapy for GI SMTs of the MP layer. Its effectiveness and safety are comparable between SMTs of the esophagus and cardia. | Chen Du Ning-Li Chai En-Qiang Ling-Hu Zhen-Juan Li Long-Song Li Jia-Le Zou Lei Jiang Zhong-Sheng Lu Jiang-Yun Meng Ping Tang | 2019 | World Journal of Gastroenterology2019，25，2： | 17 |
| 16 | A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh显示文摘Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominant Aft locus combined with the recessive atv locus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report that Aft encodes a functional anthocyanin activator named SlAN2-like, while atv encodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression of SlAN2-like is responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles of SlAN2-like and therefore fail to produce anthocyanins. Consistently, expression of a functional SlAN2-like gene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators. | Chuanlong Sun Lei Deng Minmin Du Jiuhai Zhao Qian Chen Tingting Huang Hongling Jiang Chang-Bao Li Chuanyou Li | 2020 | Molecular Plant2020，13，1： | 17 |
| 17 | Wall Imaging for Unilateral Intracranial Vertebral Artery Hypoplasia with Three-dimensional High-isotropic Resolution Magnetic Resonance Images显示文摘 | Xian-Jin Zhu Wu Wang Bin DU Lei Liu Xin-Xin He Li-Bin Hu Xue-Bin Zhang Zun-Jing Liu Wei-Jian Jiang | 2015 | Chinese Medical Journal2015，，12： | 17 |
| 18 | Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors. | Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang | 2016 | Science China(Life Sciences)2016，59，4： | 16 |
| 19 | An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis显示文摘MYB 蛋白质在真核细胞的有机体起重要作用。在植物, R1R2R3 类型 MYB 蛋白质在房间周期控制工作。然而, R2R3 类型 MYB 蛋白质是否也涉及房间部门过程,仍然保持未知。这里,我们报导那 R2R3 类型抄写因素基因, AtMYB59,涉及房间周期前进和根生长的规定。AtMYB59 蛋白质在洋葱的原子核是局部性的表皮的房间并且 transactivation 活动。在酵母房间的 AtMYB59 的表示压制房间增长,和 transformants 与更长的房间有更多的原子核和更高的 aneuploid DNA 内容。在 AtMYB59 的保存领域的变化在酵母细胞生长上废除它的效果。在同步 Arabidopsis 房间暂停, AtMYB59 基因明确地在房间周期前进期间在 S 阶段被表示。表示和 promoter-GUS 分析表明 AtMYB59 基因富有地在根被表示。转基因的植物 overexpressing AtMYB59 更短的根与野类型的植物(Arabidopsis 就职 Col-0 ) 相比,并且在在根尖端的有丝分裂的房间的一半附近在中期。相反地,空变异的 myb59-1 比关口在中期让更长的根和更少有丝分裂的房间,建议那 AtMYB59 可以由扩大有丝分裂的房间的中期禁止根生长。AtMYB59 调整许多下游的基因,包括 CYCB1; 1 基因,可能通过到 MYB 应答的元素的绑定。这些结果在细胞周期规定和植物根生长为 AtMYB59 支持一个角色。 | Rui-Ling Mu Yang-Rong Cao Yun-Feng Liu Gang Lei Hong-Feng Zou Yong Liao Hui-Wen Wang Wan-Ke Zhang Biao Ma Ji-Zhou Du Ming Yuan Jin-Song Zhang Shou-Yi Chen | 2009 | Cell Research2009，19，11： | 16 |
| 20 | Thorombolytic therapy with rescue percutaneous coronary intervention versus primary percutaneous coronary intervention in patients with acute myocardial infarction: a multicenter randomized clinical trial显示文摘背景尽管有营救的 thrombolytic 治疗经皮的冠的干预(一种总线标准)是为圣片断举起的普通治疗策略尖锐心肌的梗塞( STEMI ),欠缺的数据在它相对主要一种总线标准,和比较的功效上是可得到的因此是这 study.Methods 的目的这 multicenter ,开标签,使随机化,平行试用与 STEMI 在病人(年龄 70 年)上在 12 所医院里被进行在 12 以内介绍了症状发作的小时(吝啬的间隔> 3 个小时)。病人们被使随机化到三个组:主要一种总线标准组(n=101 ) ;recombinant staphylokinase (r-Sak ) 组(n=104 ) ;并且 recombinant 织物类型 plasminogen 使活跃之物(rt-PA ) 组(n=106 ) 。为分配到 thrombolytic 治疗手臂的所有病人,冠的 angiography 在药治疗以后在 90 分钟被执行证实梗塞相关的动脉(IRA ) 明显;营救一种总线标准与 TIMI 流动等级 2 在情况中被执行。赤裸金属的 stent 培植为所有病人被计划。 113.4 的一般水准纪录开始 thrombolytic 治疗( door-to-needle 时间)和 141.2 分钟在 IRA (到汽球时间的门)执行第一汽球通货膨胀的在它要求了的随机化以后的结果 IRA 明显( TIMI 流动等级 2 或 3 )和 TIMI 流动等级的 .Rates 3 是显著地更低的在在 90 点的 thrombolysis 组在过程的结束比在主要一种总线标准组在药治疗以后纪录(70.5%对98.0%, P < 0.0001 ,和53.0%对85.9%, P < 0.0001 ,分别地)。有 stenting 的营救一种总线标准在 thrombolytic 治疗手臂在 117 个病人(55.7%) 被执行。明显和 3 仍然是的 TIMI 流动等级的率显著地比在主要一种总线标准组在营救一种总线标准降低(88.9% 对 97.9% , P=0.0222,并且 68.4% 对 85.0% , P=0.0190,分别地) 。在 30 天治疗以后,死亡率在比在主要一种总线标准组与营救一种总线标准组相结合的 thrombolysis 是显著地更高的(7.1% 对 0, P=0.0034 ) 。 death/Ml 和流血复杂并发症的率比在主要一种总线标准组在与营救一种总线标准组一起的 thrombolysis 是显著地更高的(10.0%对1.0%, P=0.0380 ,并且28.10%对8.91%, P=0.0001 ,分别地)有营救一种总线标准的 .Conclusions Thrombolytic 治疗与冠的明显和 TIMI 流动等级的显著地更低的率被联系 3 ,但是与在 30 天的死亡, death/Ml 和出血性的复杂并发症的显著地更高的率,作为与在这组中国 S | GAO Run-lin HAN Ya-ling YANG Xin-chun MAO Jie-ming FANG Wei-yi WANG Lei SHEN Wei-feng LI Zhan-quan JIA Guo-liang LU Shu-zheng WEI Meng ZENG Ding-yin CHEN Ji-lin QIN Xue-wen XU Bo DU Chang-hui | 2010 | Chinese Medical Journal2010，，11： | 15 |
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