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| 1 | Breast cancer development and progression:Risk factors,cancer stem cells,signaling pathways,genomics,and molecular pathogenesis显示文摘As the most commonly occurring cancer in women worldwide,breast cancer poses a formidable public health challenge on a global scale.Breast cancer consists of a group of biologically and molecularly heterogeneous diseases originated from the breast.While the risk factors associated with this cancer varies with respect to other cancers,genetic predisposition,most notably mutations in BRCA1 or BRCA2 gene,is an important causative factor for this malignancy.Breast cancers can begin in different areas of the breast,such as the ducts,the lobules,or the tissue in between.Within the large group of diverse breast carcinomas,there are various denoted types of breast cancer based on their invasiveness relative to the primary tumor sites.It is important to distinguish between the various subtypes because they have different prognoses and treatment implications.As there are remarkable parallels between normal development and breast cancer progression at the molecular level,it has been postulated that breast cancer may be derived from mammary cancer stem cells.Normal breast development and mammary stem cells are regulated by several signaling pathways,such as estrogen receptors(ERs),HER2,and Wnt/b-catenin signaling pathways,which control stem cell proliferation,cell death,cell differentiation,and cell motility.Furthermore,emerging evidence indicates that epigenetic regulations and noncoding RNAs may play important roles in breast cancer development and may contribute to the heterogeneity and metastatic aspects of breast cancer,especially for triple-negative breast cancer.This review provides a comprehensive survey of the molecular,cellular and genetic aspects of breast cancer. | Yixiao Feng Mia Spezia Shifeng Huang Chengfu Yuan Zongyue Zeng Linghuan Zhang Xiaojuan Ji Wei Liu Bo Huang Wenping Luo Bo Liu Yan Lei Scott Du Akhila Vuppalapati Hue H.Luu Rex C.Haydon Tong-Chuan He Guosheng Ren | 2018 | Genes & Diseases2018，5，2： | 24 |
| 2 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 3 | Effects of irrigation water salinity on soil salt content distribution,soil physical properties and water use efficiency of maize for seed production in arid Northwest China显示文摘In order to explore the use of groundwater resources,field experiments were conducted for three consecutive years during 2012-2014 in the Shiyang River basin of Northwest China.Irrigation was conducted using four different water salinity levels that were arranged in a split plot design.These four water salinity levels were s0,s3,s6 and s9(0.71,3,6 and 9 g/L,respectively).The soil salt content,soil bulk density,soil porosity,saturated hydraulic conductivity,plant height,leaf area index and yield of maize for seed production were measured for studying the effects of saline water irrigation on soil salt content distribution,soil physical properties and water use efficiency.It was observed that higher salinity level of irrigation water and long duration of saline water irrigation resulted in more salt accumulation.Compared to initial values,the soil salt accumulation in 0-100 cm soil layer after three years of experiments for s0,s3,s6 and s9 was 0.189 mg/cm3,0.654 mg/cm3,0.717 mg/cm3 and 1.135 mg/cm3,respectively.Both greater salt levels in the irrigation water and frequent saline water irrigation led to greater soil bulk density,but poorer soil porosity and less saturated hydraulic conductivity.The saturated hydraulic conductivity decreased with increase in soil bulk density,but increased with improvement in soil porosity.It was noted that the maize height,leaf area index and maize yield gradually decreased with increase in water salinity.The maize yield decreased over 25%and the water use efficiency also gradually declined when irrigated with water containing 6 g/L and 9 g/L salinity levels.However,maize yield following saline water irrigation with 3 g/L decreased less than 20%and the decline in water use efficiency was not significant during the three-year experiment period.The results demonstrate that irrigation with saline water at the level of 6 g/L and 9 g/L in the study area is not suitable,while saline water irrigation with 3 g/L would be acceptable for a short duration together with salt leaching through spring irrigation before sowing. | Chengfu Yuan Shaoyuan Feng Juan Wang Zailin Huo Quanyi Ji | 2018 | International Journal of Agricultural and Biological Engineering2018，11，3： | 6 |
| 4 | Pioglitazone ameliorates neuronal damage after traumatic brain injury via the PPARg/NF-kB/IL-6 signaling pathway显示文摘Traumatic brain injury(TBI)is the major cause of high mortality and disability rates worldwide.Pioglitazone is an activator of peroxisome proliferator-activated receptor-gamma(PPARγ)that can reduce inflammation following TBI.Clinically,neuroinflammation after TBI lacks effective treatment.Although there are many studies on PPARγin TBI animals,only few could be converted into clinical,since TBI mechanisms in humans and animals are not completely consistent.The present study,provided a potential theoretical basis and therapeutic target for neuroinflammation treatment after TBI.First,we detected interleukin-6(IL-6),nitric oxide(NO)and Caspase-3 in TBI clinical specimens,confirming a presence of a high expression of inflammatory factors.Western blot(WB),quantitative real-time PCR(qRTPCR)and immunohistochemistry(IHC)were used to detect PPARγ,IL-6,and p-NF-kB to identify the mechanisms of neuroinflammation.Then,in the rat TBI model,neurobehavioral and cerebral edema levels were investigated after intervention with pioglitazone(PPARγactivator)or T0070907(PPARγinhibitor),and PPARγ,IL-6 and p-NF-kB were detected again by qRT-PCR,WB and immunofluorescence(IF).The obtained results revealed that:1)increased expression of IL-6,NO and Caspase-3 in serum and cerebrospinal fluid in patients after TBI,and decreased PPARγin brain tissue;2)pioglitazone could improve neurobehavioral and reduce brain edema in rats after TBI;3)the protective effect of pioglitazone was achieved by activating PPARγand reducing NF-kB and IL-6.The neuroprotective effect of pioglitazone on TBI was mediated through the PPARγ/NF-kB/IL-6 pathway. | Yongbing Deng Xue Jiang Xiaoyan Deng Hong Chen Jie Xu Zhaosi Zhang Geli Liu Zhu Yong Chengfu Yuan Xiaochuan Sun Changdong Wang | 2020 | Genes & Diseases2020，7，2： | 6 |
| 5 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 6 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 7 | Fluid migration paths in the marine strata of typical structures in the western Hubei-eastern Chongqing area,China显示文摘The western Hubei-eastern Chongqing area is an important prospective zone for oil and gas exploration in the central Yangtze area. Three representative structures, the Xinchang structure, Longjuba gas-bearing structure and the Jiannan gas field, were selected to analyze biomarker parameters in marine strata and to examine various types of natural gas and hydrocarbon sources. Fluid inclusions; carbon, oxygen, and strontium isotopic characteristics; organic geochemical analysis and simulation of hydrocarbon generation and expulsion history of source rocks were used for tracing fluid migration paths in marine strata of the study area. The Carboniferous-Triassic reservoirs in three typical structures all experienced at least two stages of fluid accumulation. All marine strata above the early Permian were shown to have fluids originating in the Permian rocks, which differed from the late stage fluids. The fluids accumulated in the late Permian reservoirs of the Xinchang structure were Cambrian fluids, while those in the late Carboniferous reservoirs were sourced from a combination of Silurian and Cambrian fluids. A long-distance and large-scale cross-formational flow of fluids destroyed the preservation conditions of earlier accumulated hydrocarbons. A short-distance cross-formational accumulation of Silurian fluids was shown in the late Permian reservoirs of the Longjuba structure with favorable hydrocarbon preservation conditions. The fluid accumulation in the Carboniferous reservoirs of the Jiannan structure mainly originated from neighboring Silurian strata with a small amount from the Cambrian strata. As a result, the Jiannan structure was determined to have the best preservation conditions of the three. Comparative analysis of fluid migration paths in the three structures revealed that the zone with a weaker late tectonism and no superimposition and modification of the Upper and Lower Paleozoic fluids or the Upper Paleozoic zone with the fluid charging from the Lower Paleozoic in the western Hubei-eastern Chongqing area are important target areas for future exploration. | Xu Guosheng Liang Jiaju Gong Deyu Wang Guozhi Yuan Haifeng Cao Junxing Zhang Chengfu | 2013 | Petroleum Science2013，10，1： | 3 |
| 8 | Some properties of contraction critical 5-connected graphs显示文摘 | QIN Chengfu YUAN Xudong SU Jianji | 2008 | Discrete Mathematics2008，308，： | 1 |
| 9 | Evaluation of antioxidant and immune activity of Phellinus ribis glucan in mice显示文摘 | Chengfu Yuan Xiuning Huang Li Cheng Youquan Bu Geli Liu Faping Yi Zhengmei Yang Fangzhou Song | 2008 | Food Chemistry2008，，2： | 1 |
| 10 | Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice显示文摘 | Chengfu Yuan Changdong Wang Youquan Bu Tingxiu Xiang Xiuning Huang Zhiwei Wang Faping Yi Guosheng Ren Geli Liu Fangzhou Song | 2010 | Immunology Letters2010，，1： | 1 |
| 11 | Simulation and optimization of irrigation schedule for summer maize based on SWAP model in saline region显示文摘In order to explore the appropriate irrigation schedule for summer maize,a field experiment was conducted in 2013 in Lubotan of Shaanxi Province.Soil water content,soil salinity,soil hydraulic parameters,crop growth parameters and summer maize yield were measured in the experiment.The SWAP model was calibrated based on field experiment observation data in 2013.The SWAP model was used to simulate and optimize irrigation schedule for summer maize after calibration.The results showed that model simulation results of soil water content,soil salinity and summer maize yield agreed well with the measured values.The Root Mean Square Error(RMSE)and Mean Relative Error(MRE)were within the allowable error ranges.The RMSE values were all lower than 0.05 cm3/cm3 and the MRE values were lower than 15%in soil water content calibration.The RMSE values were all lower than 0.1 mg/cm3 and the MRE values were lower than 20%in soil salinity calibration.The RMSE and MRE values were 1299.6 kg/hm2 and 15.26%in summer maize yield calibration.The model parameters suitable for the study area were obtained in calibration.The SWAP model could be used to simulate and optimize irrigation schedule for summer maize after calibration.The SWAP model was used to simulate soil water-salt balance,summer maize yield and water use efficiency under different irrigation schedules.The model simulation results for different irrigation schedules indicated that the optimal irrigation schedules of summer maize were three times each for jointing stage(July 5),heading stage(August 5)and grain filling stage(August 30)with irrigation amount of 128 mm,128 mm and 96 mm,respectively.The optimal irrigation quota was 352.0 mm for summer maize in the study area. | Yanxin Pan Chengfu Yuan Siyuan Jing | 2020 | International Journal of Agricultural and Biological Engineering2020，13，3： | 1 |
| 12 | Critical roles of the lncRNA CASC11 in tumor progression and cancer metastasis:The biomarker and therapeutic target potential显示文摘The frequency of human suffering from cancer is increasing annually across the globe.This has fueled numerous investigations aimed at the prevention and cure of various cancers.Long non-coding RNA(lncRNA)are known to play a crucial role in cancer.For instance,cancer susceptibility candidate 11(CASC11),as one of the long non-coding RNAs,has been reported to be overexpressed in various tumors.This review elucidates the mechanism by which lncRNA CASC11 regulates tumors'biological processes and affirms its value as a therapeutic target for tumors.Through systematic analysis and review of relevant articles in PubMed,we revealed the pathophysiological mechanism of CASC11 on the tumor by regulating the biological processes of tumor such as proliferation,autophagy,apoptosis,thereby promoting tumor metastasis.We also revealed the regulatory pathways of CASC11 in different tumors,for instance by acting on a variety of microRNAs,oncogenic proteins,carcinogens,and transcription factors.Consequently,CASC11 regulates cancer proliferation,apoptosis,and invasion by altering the WNT/β-catenin signaling pathway and epithelial–mesenchymal transition(EMT).Furthermore,CASC11 expression has a high pertinence with clinical prognosis,suggesting that it is a potential marker for malignant tumors or a clinical adjuvant therapy in the future. | Bei Wang Wen Xu Chengyu Hu Kai Liu Jinlan Chen Chong Guo Chengfu Yuan | 2022 | Genes & Diseases2022，9，2： | 1 |
| 13 | Evaluation of antioxidant and immune activity of Phellinus ribis glucan in mice显示文摘 | Yuan Chengfu Huang Xiuning Cheng Li | 2009 | Food Chemistry2009，115，2： | 1 |
| 14 | Antioxidative and immunoprotective effects of Pyracantha fortuneana (Maxim.) Li polysaccharides in mice显示文摘 | Chengfu Yuan Changdong Wang Youquan Bu Tingxiu Xiang Xiuning Huang Zhiwei Wang Faping Yi Guosheng Ren Geli Liu Fangzhou Song | 2010 | Immunology Letters2010，，1： | 1 |
| 15 | Chinese herbal decoction astragalus and angelica exerts its therapeutic effect on renal interstitial fibrosis through the inhibition of MAPK,PI3K-Akt and TNF signaling pathways显示文摘Astragalus and Angelica decoction(A&A)has been clinically used as a classical traditional Chinese medicine(TCM)formula in China for many years for the treatment of kidney diseases,especially renal interstitial fibrosis(RIF).However,the mechanisms underlying the therapeutic effects of A&A on RIF remains poorly understood.In the present study,systematic network pharmacology and effective experimental verification were utilized for the first time to elucidate the pharmacological efficacy and potential mechanism.The outcomes indicated that 22 active components and 87 target genes of A&A were identified and cross-referenced with RIF-associated genes,contributing to confirmation of 74 target genes of A&A for RIF.Pathway and functional enrichment analyses revealed that A&A had substantial effects on MAPK,PI3K-Akt and TNF signaling pathways.In addition,seven core targets with relatively higher betweenness and degree were identified in the constructed Chinese medicine material-chemical component-target-signal pathway network.Moreover,we verified the potential therapeutic effect of A&A in vivo(using a mouse model of RIF),confirming that A&A could effectively protect the kidney by regulating these target genes.The therapeutic effect of A&A on RIF could be attributed to its role in regulating the cell cycle,limiting the apoptosis,and inhibiting the inflammation. | Hao Yuan Xuelian Wu Xiaomin Wang Chengfu Yuan | 2022 | Genes & Diseases2022，9，2： | 1 |
| 16 | Evaluation of antioxidant and immune activity of Phellinus ribis gluean in mice显示文摘 | YUAN Chengfu HUANG Xiuning CHENG Li | 2009 | Food Chemistry2009，115，： | 1 |
| 17 | Evaluation of antioxidant and immune activity of Phellinus ribis glucan in mice显示文摘 | YUAN CHENGFU HUANG XIUNING CHENG LI | 2009 | Food Chemistry2009，，115： | 1 |
| 18 | Some Properties of contraction-critical 5-connected graphs显示文摘 | QIN Chengfu YUAN Xudong SU Jianji | 2008 | Discrete Mathematics2008，，308： | 1 |
| 19 | Triangles in contraction critical 5-connected graphs显示文摘 | QIN Chengfu YUAN Xudong SU Jianji | 2005 | Australian Journal of combinatorics2005，，133： | 1 |
| 20 | Some properties of contraction critical 5-connected graphs显示文摘 | QIN Chengfu YUAN Xudong SU Jianji | 2008 | Discrete Mathematics2008，308，： | 1 |
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