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| 1 | Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight显示文摘谷物重量是庄稼谷物产量的一个主要决定因素并且被自然地发生的量的特点 loci (QTL ) 控制。我们更早识别了控制米饭谷物宽度和重量的主要 QTL, GW5,它在米饭染色体 5 上被印射到一个再结合热点。获得 GW5 怎么控制米饭谷物宽度的更好的理解,我们进行了这个地点的好印射并且揭开在米饭栽培变种 Asominori 与增加的谷物宽度联系的 1 212-bp 删除,与苗条谷物米饭 IR24 比较。另外, 46 米饭栽培变种的 genotyping 分析表明这删除高度与谷物宽度显型被相关,建议 GW5 删除可能在米饭驯服期间被选择了。GW5 编码对原子核局部性的 144 氨基酸的新奇原子蛋白质。而且,我们证明 GW5 身体上在酵母与 polyubiquitin 交往二混血儿的试金。一起,我们的结果建议 GW5 代表主要 QTL 内在的米饭宽度和重量,并且它多半在 ubiquitin-proteasome 小径行动在种子开发期间调整房间分割。这研究提供新奇卓见进控制米饭谷物开发的分子的机制并且建议 GW5 能为庄稼的产量很高的繁殖用作一个潜在的工具。 | Jianfeng Weng Suhai Gu Xiangyuan Wan He Gao Tao Guo Ning Su Cailin Lei Xin Zhang Zhijun Cheng Xiuping Guo Jiulin Wang Ling Jiang Huqu Zhai Jianmin Wan | 2008 | Cell Research2008，18，12： | 248 |
| 2 | Prevalence of Nontraumatic Osteonecrosis of the Femoral Head and its Associated Risk Factors in the Chinese Population: Results from a Nationally Representative Survey显示文摘 | De-Wei Zhao Mang Yu Kai Hu Wei Wang Lei Yang Ben-Jie Wang Xiao-Hong Gao Yong-Ming Guo Yong-Qing Xu Yu-Shan Wei Si-Miao Tian Fan Yang Nan Wang Shi-Bo Huang Hui Xie Xiao-Wei Wei Hai-Shen Jiang Yu-Qiang Zang Jun Ai Yuan-Liang Chen Guang-Hua Lei Yu-Jin Li Geng Tia Zong-Sheng Li Yong Cao Li Ma | 2015 | Chinese Medical Journal2015，，21： | 151 |
| 3 | Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors显示文摘 | Jing Liao Zhao Wu Ying Wang Lu Cheng Chun Cui Yuan Gao Taotao Chen Lingjun Rao Siye Chen Nannan Jia Huiming Dai Shunmei Xin Jiuhong Kang Gang Pei Lei Xiao | 2008 | Cell Research2008，18，5： | 61 |
| 4 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 5 | Assessing the health risk of heavy metals in vegetables to the general population in Beijing,China显示文摘A systematic survey of As, Cd, Cr, Cu, Ni, Pb and Zn concentrations in vegetables from 416 samples (involving 100 varieties) in Beijing was carried out for assessing the potential health risk to local inhabitants. The results indicated that the metal concentrations in vegetables ranged from < 0.001 to 0.479 μg/g fresh weight (fw) (As), < 0.001 to 0.101 μg/g fw (Cd), < 0.001 to 1.04 μg/g fw (Cr), 0.024 to 8.25 μg/g fw (Cu), 0.001 to 1.689 μg/g fw (Ni), < 0.001 to 0.655 μg/g fw (Pb) and 0.01 to 25.6 μg/g fw (Zn), with average concentrations of 0.013, 0.010, 0.023, 0.51, 0.053, 0.046 and 2.55 μg/g fw, respectively. The results showed that the concentrations of As, Cr, Cu, Cd, Pb and Ni in vegetables from open-fields were all significantly higher than those grown in greenhouses. In addition, in local-produced vegetables, all HMs except Zn were significantly higher than those in provincial vegetables. The estimated daily intake (EDI) of As, Cd, Cr, Cu, Ni, Pb and Zn from vegetables was 0.080, 0.062, 0.142, 3.14, 0.327, 0.283 and 15.7 μg/(kg body weight (bw)·d) for adults, respectively. Arsenic was the major risk contributor for inhabitants since the target hazard quotient based on the weighted average concentration (THQw) of arsenic amounted to 44.3% of the total THQ (TTHQ) value according to average vegetable consumption. The TTHQ was lower than 1 for all age groups, indicating that it was still safe for the general population of Beijing to consume vegetables. | SONG Bo LEI Mei CHEN Tongbin ZHENG Yuanming XIE Yunfeng LI Xiaoyan GAO Ding | 2009 | Journal of Environmental Sciences2009，21，12： | 45 |
| 6 | Ligustrazine monomer against cerebral ischemia/reperfusion injury显示文摘Ligustrazine(2,3,5,6-tetramethylpyrazine) is a major active ingredient of the Szechwan lovage rhizome and is extensively used in treatment of ischemic cerebrovascular disease.The mechanism of action of ligustrazine use against ischemic cerebrovascular diseases remains unclear at present.This study summarizes its protective effect,the optimum time window of administration,and the most effective mode of administration for clinical treatment of cerebral ischemia/reperfusion injury.We examine the effects of ligustrazine on suppressing excitatory amino acid release,promoting migration,differentiation and proliferation of endogenous neural stem cells.We also looked at its effects on angiogenesis and how it inhibits thrombosis,the inflammatory response,and apoptosis after cerebral ischemia.We consider that ligustrazine gives noticeable protection from cerebral ischemia/reperfusion injury.The time window of ligustrazine administration is limited.The protective effect and time window of a series of derivative monomers of ligustrazine such as 2-[(1,1-dimethylethyl)oxidoimino]methyl]-3,5,6-trimethylpyrazine,CXC137 and CXC195 after cerebral ischemia were better than ligustrazine. | Hai-jun Gao Peng-fei Liu Pei-wen Li Zhuo-yan Huang Feng-bo Yu Ting Lei Yong Chen Ye Cheng Qing-chun Mu Hai-yan Huang | 2015 | Neural Regeneration Research2015，10，5： | 48 |
| 7 | TRICHOMEAND ARTEMISININ REGULATOR 1 Is Required for Trichome Development and Artemisinin Biosynthesis in Artemisia annua显示文摘毛状体,许多植物种类的表面上的小伸出,能生产并且存储各种各样的第二等的新陈代谢的产品。Artemisinin,最著名并且为疟疾的有势力药,被综合,存储,并且由 Artemisia annua 毛状体藏匿了。然而,糟糕在 A.annua 遗体调整 artemisinin 的生合成和毛状体的发展的分子的基础理解。这里,我们报导那一个 AP2 抄写因素,毛状体和 ARTEMISININ 管理者 1 (TAR1 ) ,在在 A.annua 调整毛状体的发展和 artemisinin 的生合成起关键作用。TAR1,编码特殊位于原子核的蛋白质,主要在年轻叶子,花芽,和一些毛状体被表示。在 TAR1-RNAi 线,毛状体的形态学和表皮的蜡的作文被改变,并且 artemisinin 内容戏剧性地被减少它能被 TAR1 oeverexpression 显著地增加。当 TAR1 是 silenced 或 overexpressed 时,涉及 artemisinin 生合成的几关键基因的表示层次被改变。由 electrophoretic 活动性移动,酵母一个混血儿和短暂转变 -glucuronidase 试金,我们证明广告和 CYP71AV1,在 artemisinin 的生合成小径的二关键基因,是可能的 TAR1 的直接目标。一起拿,我们的结果显示 TAR1 是在 A.annua 调整毛状体开发和 artemisinin 生合成的分子的网络的一个关键部件。 | Hexin Tan Ling Xiao Shouhong Gao Qing Li Junfeng Chen Ying Xiao Qian Ji Ruibing Chen Wansheng Chen Lei Zhang | 2015 | Molecular Plant2015，8，9： | 37 |
| 8 | LncRNA TUG1 influences papillary thyroid cancer cell proliferation, migration and EMT formation through targeting miR-145显示文摘LncRNA TUG1,与各种各样的人的癌症联系的肿瘤 oncogene,被报导了涉及调整各种各样的细胞的过程例如增长,通过指向多重基因的 apoptosis 和侵略。然而,它在甲状腺癌症房间的生物功能没被阐明。这研究的目的是测量 TUG1 表示水平并且在甲状腺癌症房间评估它的功能。在甲状腺癌症纸巾和三根甲状腺癌症房间线的 LncRNA TUG1 表示层次(ATC 房间线 SW1736 和 KAT18 和 FTC 房间衬里 FTC133 ) 被 qRT-PCR 并且与人的正常的胸的相比估计上皮的房间 HGC-27。MTT 试金,殖民地形成试金, transwell 试金和西方的污点分析被执行在 vitro 在甲状腺癌症房间在增长,转移和 EMT 形成上估计 TUG1 的效果。营救试金被执行进一步证实 TUG1 通过调整 miR-145/ZEB1 信号小径贡献甲状腺癌症房间的前进。LncRNA TUG1 被发现在人的正常的胸与那相比在甲状腺癌症纸巾和甲状腺癌症房间起来调整上皮的房间 HGC-27。增加的 lncRNA TUG1 表示被发现显著地支持肿瘤房间增长,并且当下面调整的 TUG1 能显然禁止房间增长,迁居 / 侵略和反向的 EMT 到时,便于房间侵略相遇。这些结果显示 TUG1 可以作为 ceRNA 由功能贡献甲状腺癌症房间的前进竞争擦掉 miR-145,和那 lncRNA TUG1 在甲状腺癌症房间与肿瘤前进被联系。 | Hongwei Lei Yan Gao Xiaoying Xu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 37 |
| 9 | Chemical composition and source apportionment of PM10 and PM2.5 in different functional areas of Lanzhou,China显示文摘To elucidate the air pollution characteristics of northern China, airborne PM_10(atmospheric dynamic equivalent diameter ≤ 10 μm) and PM_(2.5)(atmospheric dynamic equivalent diameter ≤ 2.5 μm) were sampled in three different functional areas(Yuzhong County,Xigu District and Chengguan District) of Lanzhou, and their chemical composition(elements, ions, carbonaceous species) was analyzed. The results demonstrated that the highest seasonal mean concentrations of PM_10(369.48 μg/m^3) and PM_(2.5)(295.42 μg/m^3) were detected in Xigu District in the winter, the lowest concentration of PM_(2.5)(53.15 μg/m^3) was observed in Yuzhong District in the fall and PM_10(89.60 μg/m^3) in Xigu District in the fall.The overall average OC/EC(organic carbon/elemental carbon) value was close to the representative OC/EC ratio for coal consumption, implying that the pollution of Lanzhou could be attributed to the burning of coal. The content of SNA(the sum of sulfate, nitrate,ammonium, SNA) in PM_(2.5)in Yuzhong County was generally lower than that at other sites in all seasons. The content of SNA in PM_(2.5)and PM_10 in Yuzhong County was generally lower than that at other sites in all seasons(0.24–0.38), indicating that the conversion ratios from precursors to secondary aerosols in the low concentration area was slower than in the area with high and intense pollutants. Six primary particulate matter sources were chosen based on positive matrix factorization(PMF) analysis, and emissions from dust, secondary aerosols, and coal burning were identified to be the primary sources responsible for the particle pollution in Lanzhou. | Xionghui Qiu Lei Duan Jian Gao Shulan Wang Fahe Chai Jun Hu Jingqiao Zhang Yaru Yun | 2016 | Journal of Environmental Sciences2016，28，2： | 33 |
| 10 | Broadband diffusion of terahertz waves by multi-bit coding metasurfaces显示文摘The terahertz region is a special region of the electromagnetic spectrum that incorporates the advantages of both microwaves and infrared light waves.In the past decade,metamaterials with effective medium parameters or gradient phases have been studied to control terahertz waves and realize functional devices.Here,we present a new approach to manipulate terahertz waves by using coding metasurfaces that are composed of digital coding elements.We propose a general coding unit based on a Minkowski closed-loop particle that is capable of generating 1-bit coding(with two phase states of 0 and 180°),2-bit coding(with four phase states of 0,90°,180°,and 270°),and multi-bit coding elements in the terahertz frequencies by using different geometric scales.We show that multi-bit coding metasurfaces have strong abilities to control terahertz waves by designing-specific coding sequences.As an application,we demonstrate a new scattering strategy of terahertz waves—broadband and wide-angle diffusion—using a 2-bit coding metasurface with a special coding design and verify it by both numerical simulations and experiments.The presented method opens a new route to reducing the scattering of terahertz waves. | Li-Hua Gao Qiang Cheng Jing Yang Shao-Jie Ma Jie Zhao Shuo Liu Hai-Bing Chen Qiong He Wei-Xiang Jiang Hui-Feng Ma Qi-Ye Wen Lan-Ju Liang Biao-Bing Jin Wei-Wei Liu Lei Zhou Jian-Quan Yao Pei-Heng Wu Tie-Jun Cui | 2015 | Light(Science & Applications)2015，4，1： | 31 |
| 11 | Analyses of hot and humid weather in Beijing city in summer and its dynamical identification显示文摘The circulation, hygrothermal property and moisture transport character of typical hot and humid weather were analyzed in Beijing areas from July 30 to August 4, 2002. It was pointed out that, under the control of subtropical anticyclone which stretches to the west and north, downdraft suppresses the lifting of lower-troposphere moisture, which makes moisture keep in the lower troposphere. That is the direct reason causing hot and humid weather. Considering the non-uniformity saturated character in real atmosphere, generalized moist potential vorticity (GMPV) equation is derived by the introduction of generalized moist potential temperature concept. The analysis of GMPV shows that negative GMPV anomaly occurs in the lower troposphere. It has indicative sense to hot and humid weather. Thus, the GMPV anomaly can be utilized to identify this kind of weather and to make a short-term prediction. | GAO Shouting ZHOU Yushu LEI Ting SUN Jianhua | 2005 | Science China Earth Sciences2005，48，z2： | 30 |
| 12 | Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products显示文摘最近的婴儿的公式奶粉污染事件证明了在牛奶质量控制的经典标记或标准在识别鈥渕a nipulated 鈥 ? 是不够的差质量的牛奶。在现在的学习,我们第一次证明奶牛牛奶包含大量 microRNAs ( miRNAs )并且牛奶特定的 miRNAs 的唯一的表示侧面能为未加工的牛奶和牛奶相关的商业产品的质量控制用作新奇指示物和可能的新标准,例如液体牛奶和粉状的公式牛奶。用定序的 Solexa,首先,我们系统地在未加工的牛奶屏蔽了 miRNA 表示并且在未加工的牛奶识别了 245 miRNAs 的一个总数。不同于层次在哺乳的不同时期是其表达式将近相同的另外的经典 biomarkers,单个 miRNAs 能显著地在哺乳过程期间被改变,含有那 miRNAs 可以是更精确的指示物反映牛奶的优秀改变。第二,使用 TaqMan 基于探查的 miRNA 量的 RT-PCR ,我们进一步识别了七 miRNAs 重要地在整个哺乳过程,和更多有相对一致的表情,这七牛奶特定的 miRNAs 的表示侧面能为区别用作理想的 biomarker 差质量或从纯未加工的牛奶的 鈥渕a nipulated 鈥?牛奶,象为商业乳制品的质量控制一样,例如液体,牛奶和粉状的公式挤。一起,我们的调查结果为为决定未加工的牛奶或牛奶相关的商业产品的质量理解牛奶 miRNAs 和一个新潜在的标准的生理的角色提供一个基础。 | Xi Chen Chao Gao Haijin Li Lei Huang Qi Sun Yanye Dong Chunliang Tian Shengpu Gao Hailin Dong Danping Guan Xiaoyun Hu Shujian Zhao Liang Li Lin Zhu Qiao Yan Junfeng Zhang Ke Zen Chen-Yu Zhang | 2010 | Cell Research2010，20，10： | 29 |
| 13 | Stenting for symptomatic intracranial arterial stenosis in China:1-year outcome of a multicentre registry study显示文摘background and purpose A multicentre prospective registry study of individually tailored stenting for a patient with symptomatic intracranial atherosclerotic stenosis(ICAS)combined with poor collaterals in China showed that the short-term safety and efficacy of stenting was acceptable.However,it remained uncertain whether the low event rate could be of a long term.We reported the 1-year outcome of this registry study to evaluate the long-term efficacy of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Methods Patients with symptomatic ICAS caused by 70%-99% stenosis located at the intracranial internal carotid,middle cerebral,intracranial vertebral or basilar arteries combined with poor collaterals were enrolled.Balloon-mounted stent or balloon plus self-expanding stent were selected based on the ease of vascular access and lesion morphology determined by the operators.The primary outcome was the rate of 30-day stroke,transient ischaemic attack and death,and 12-month ischaemic stroke within the same vascular territory,haemorrhagic stroke and vascular death after stenting.results From September 2013 to January 2015,300 patients(ages 58.3±9.78 years)were recruited.Among them,159 patients were treated with balloon-mounted stent and 141 with balloon plus self-expanding stent.During the 1-year follow-up,25 patients had a primary end point event.The probability of primary outcome at 1 year was 8.1%(95% CI 5.3% to 11.7%).In 76 patients with digital subtraction angiography follow-up,27.6%(21/76)had re-stenosis≥50% and 18.4%(14/76)had re-stenosis≥70%.No baseline characteristic was associated with the primary outcome.Conclusion The event rate remains low over 1 year of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Further randomised trial of comparing individually tailored stenting with best medical therapy is needed. | Ning Ma Yong Zhang Jie Shuai Changchun Jiang Qiyi Zhu Kangning Chen Li Liu Baomin Li Xiangqun Shi Lianbo Gao Yajie Liu Feng Wang Yongli Li Tieyan Liu Hongbo Zheng Dapeng Mo Feng Gao Yilong Wang Yongjun Wang Lei Feng Zhongrong Miao | 2018 | Stroke & Vascular Neurology2018，3，3： | 29 |
| 14 | Epidemiology, genetics and treatments for myopia显示文摘· Myopia is a significant public health problem and its prevalence is increasing over time and genetic factors in disease development are important. The prevalence and incidence of myopia within sampled population often varies with age, country, sex, race, ethnicity, occupation, environment, and other factors. Myopia growth is under a combination of genes and their products in time and space to complete the coordination role of the guidance. Myopia-related genes include about 70 genetic loci to which primary myopias have been mapped, although the number is constantly increasing and depends to some extent on definition. Of these, several are associated with additional abnormalities, mostly as part of developmental syndromes. These tend to result from mutations in genes encoding transcriptional activators, and most of these have been identified by sequencing candidate genes in patients with developmental anomalies. Currently, collagen alpha-1 chain of type I(COL1A1), collagen alpha-1 chain of type II(COL2A1), actin, alpha, cardiac muscle 1(ACTC1), paired box gene 6(PAX6) and NIPBL (nipped-B homolog), and so on have been mapped. Myopia is most commonly treated with spectacles or glasses. The most common surgical procedure performed to correct myopia is laser keratomileusis (LASIK). This review of the recent advances on epidemiology, genetic locations and treatments of myopia are summarized. · | Lei Yu, Chang-Tai Xu | 2011 | International Journal of Ophthalmology(English edition)2011，4，6： | 27 |
| 15 | Telomerase activity in gastric cancer and its clinical implications显示文摘INTRODUCTIONThedevelopmentofcarcinomaresultsfrommultipleindependentgeneticchangesthatactivateprotooncogenesorinactivatetheac... | ZHAN Wen Hua, MA Jin Ping, PENG Jun Sheng, GAO Jing Song, CAI Shi Rong, WANG Jian Ping, ZHENG Zhang Qing and WANG Lei | 1999 | World Journal of Gastroenterology1999，5，4： | 25 |
| 16 | Tonoplast calcium sensors CBL2 and CBL3 control plant growth and ion homeostasis through regulating V-ATPase activity in Arabidopsis显示文摘对发展、环境的暗示的植物回答被钙经常调停(被多样的钙传感器播送的 Ca 2+) 信号。calcineurin 象 B 一样(CBL ) 蛋白质家庭代表通过和一组交往 CBL 蛋白质 kinases 的特定的相互作用译码钙信号的钙传感器。我们报导 Arabidopsis CBL2 和 CBL3 的功能的分析,对通过 N 终端指向 tonoplast 的 vacuolar 膜局部性的二个密切相关的 CBL 成员定序。当 cbl2 或 cbl3 单身者异种没从野类型显示出任何 phenotypic 差别时, cbl2 cbl3 两倍异种与叶尖端坏死,不发达的根,更短的 siliques 和更少种子被阻碍。这些缺点在 vha-a2 vha-a3 使人想起那些在 vacuolar H +-ATPase (V-ATPase ) 缺乏的两倍异种。确实, V-ATPase 活动在 cbl2 cbl3 被减少两倍变异的、连接 tonoplast CBL 类型钙传感器到 V-ATPase 的规定。而且, cbl2 cbl3 两倍异种在离子的忍耐和微量元素累积被损害,与在被显示了在离子区分工作的 V-ATPase 活动的缺点一致。我们的结果建议钙传感器 CBL2 和 CBL3 用作在钙发信号和 V-ATPase 之间的分子的连接,细胞内部的离子动态平衡的一个中央管理者。 | Ren-Jie Tang Hua Liu Yang Yang Lei Yang Xiao-Shu Gao Veder J Garcia Sheng Luan Hong-Xia Zhang | 2012 | Cell Research2012，22，12： | 26 |
| 17 | VGLL4 functions as a new tumor suppressor in lung cancer by negatively regulating the YAP-TEAD transcriptional complex显示文摘肺癌症是与高发生和死亡世界范围的大多数破坏疾病之一。河马(Hpo ) 小径是在果蝇和哺乳动物的机关尺寸的一个保存管理者。新兴的证据在癌症开发建议了 Hpo 小径的意义。在这研究,我们通过否定地调整 YAP-TEAD 建筑群的形成在肺 carcinogenesis 作为新奇肿瘤 suppressor 识别 VGLL4, Hpo 的核心部件小径。我们 VGLL4 经常被观察低层的数据表演在老鼠和人的肺癌症标本表示了。VGLL4 的宫外的表示显著地在 vitro 压制肺癌症房间的生长。更重要地, VGLL4 显著地在 de novo 老鼠模型禁止肺癌症前进。我们进一步发现 VGLL4 禁止 YAP-TEAD transcriptional 建筑群的活动。我们 VGLL4 直接在绑定与笨蛋竞争到 TEAD 并且执行的数据表演它通过二 TDU 域的生长禁止的函数。一起,我们的学习证明 VGLL4 是为通过否定地调整 YAP-TEAD 复杂形成并且这样的肺癌症的新奇肿瘤 suppressor Hpo 小径。 | Wenjing Zhang Yijun Gao Peixue Li Zhubing Shi Tong Guo Fei Li Xiangkun Han Yan Feng Chao Zheng Zuoyun Wang Fuming Li Haiquan Chen Zhaocai Zhou Lei Zhang Hongbin Ji | 2014 | Cell Research2014，24，3： | 25 |
| 18 | Calycosin improves cognitive function in a transgenic mouse model of Alzheimer's disease by activating the protein kinase C pathway显示文摘The major pathological changes in Alzheimer's disease are beta amyloid deposits and cognitive impairment. Calycosin is a typical phytoestrogen derived from radix astragali that binds to estrogen receptors to produce estrogen-like effects. Radix astragali Calycosin has been shown to relieve cognitive impairment induced by diabetes mellitus, suggesting calycosin may improve the cognitive function of Alzheimer's disease patients. The protein kinase C pathway is upstream of the mitogen-activated protein kinase pathway and exerts a neuroprotective effect by regulating Alzheimer's disease-related beta amyloid degradation. We hypothesized that calycosin improves the cognitive function of a transgenic mouse model of Alzheimer's disease by activating the protein kinase C pathway. Various doses of calycosin(10, 20 and 40 mg/kg) were intraperitoneally injected into APP/PS1 transgenic mice that model Alzheimer's disease. Calycosin diminished hippocampal beta amyloid, Tau protein, interleukin-1 beta, tumor necrosis factor-alpha, acetylcholinesterase and malondialdehyde levels in a dose-dependent manner, and increased acetylcholine and glutathione activities. The administration of a protein kinase C inhibitor, calphostin C, abolished the neuroprotective effects of calycosin including improving cognitive ability, and anti-oxidative and anti-inflammatory effects. Our data demonstrated that calycosin mitigated oxidative stress and inflammatory responses in the hippocampus of Alzheimer's disease model mice by activating the protein kinase C pathway, and thereby improving cognitive function. | Lei Song Xiaoping Li Xiao-xue Bai Jian Gao Chun-yan Wang | 2017 | Neural Regeneration Research2017，12，11： | 25 |
| 19 | ZIKA virus isolated from mosquitoes:a field and laboratory investigation in China,2016显示文摘A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells(C6/36) and mammalian cells(BHK-21) for virus isolation. The results indicated that 40%(3,540/8,795) were Anopheles sinensis, 30%(2,700/8,795) were Culex pipiens quinquefasciatus, and 29%(2,530/8,795)were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses(ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses(JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses(BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus(DNV) isolated from Culex pipiens quinquefasciatus.The ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in China's Mainland. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue. | Shihong Fu Song Song Hong Liu Yuanyuan Li Xiaolong Li Xiaoyan Gao Ziqian Xu Guoping Liu Dingming Wang Zhenzao Tian Jingzhu Zhou Ying He Wenwen Lei Huanyu Wang Bin Wang Xiaoqing Lu Guodong Liang | 2017 | Science China(Life Sciences)2017，60，12： | 25 |
| 20 | Modified B-ultrasound method for measurement of antral section only to assess gastric function and guide enteral nutrition in critically ill patients显示文摘AIM To establish a modified B-ultrasound method of measuring the antral section only to assess gastric motility in healthy people,and evaluate its application in guiding enteral nutrition(EN) in critically ill patients. METHODS First,30 healthy volunteers were selected. The modified B-ultrasound method and the traditional B-ultrasound method were applied to assess gastric function. The correlation of indices of gastric function between the two groups was analyzed statistically. In addition,64 critically ill patients were selected,and the modified B-ultrasound method and the gastric juice withdrawal method were applied to guide the implementation of EN. Daily caloric value,the time required to achieve complete EN,ICU stay,hospitalization time,and serum prealbumin and albumin levels were recorded and compared between the two groups. Kaplan-Meier survival curve was used to compare the complications of EN between the two groups. RESULTS In healthy subjects,there was a good correlation among gastric emptying time,antral contraction frequency andantral motility index between the two groups(r = 0.57,0.61 and 0.54,respectively). The study on critically ill patients also revealed that a better effect of EN was achieved in the modified B-ultrasound method group,in which patients had shorter ICU stay and hospitalization time and higher levels of serum prealbumin and albumin. The Kaplan-Meier survival analysis revealed that the improved B-ultrasound method was associated with significantly fewer EN complications(P = 0.031).CONCLUSION The modified B-ultrasound method can provide a good real-time assessment of gastric function and has a better effect than the traditional method in guiding EN in critically ill patients. | Ying Liu Ya-Kun Gao Lei Yao Li Li | 2017 | World Journal of Gastroenterology2017，23，28： | 24 |
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