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| 1 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 2 | Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome显示文摘Hutchinson-Gilford 早衰症候群(HGPS ) 和沃纳症候群(WS ) 是二最好描绘的人的 progeroid 症候群。HGPS 被一个点变化在 lamin A (LMNA ) 基因引起,导致截断的蛋白质 productprogerin 的生产。WS 被变化在 WRN 基因引起,编码 loss-of-function RecQ DNA helicase。这里,由基因编辑,我们创造了 isogenic 人的胚胎的干细胞(转换字符) 与异质接合(G608G/+) 或同型结合(G608G/G608G ) LMNA 变化和 biallelic WRN 大美人为为 HGPS 和 WS 致病建模,分别地。当转换字符和 endothelial 房间(EC ) 没介绍早熟的老朽的任何特征时, HGPS 间充质、 WS 间充质的干细胞(MSC ) 与不同动力学显示出联系老化的显型。当 HGPS-MSCs 展出了迟了发作的尖锐早衰 characterisitcs 时, WS-MSCs 有早发作的温和早衰显型。一起拿,我们的学习比较并且形成对照不同病理 underpinning 二早衰混乱,并且提供可靠干细胞为病理学、生理的老化识别新治疗学的策略的基于的模型。 | Zeming Wu Weiqi Zhang Moshi Song Wei Wang Gang Wei Wei Li Jinghui Lei Yu Huang Yanmei Sang Piu Chan Chang Chen Jing Qu Keiichiro Suzuki Juan Carlos Izpisua Belmonte Guang-Hui Liu | 2018 | Protein & Cell2018，9，4： | 15 |
| 3 | Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer显示文摘Metastasis,the development of secondary malignant growths at a distance from a primary tumor,is the cause of death for 90%of cancer patients,but little is known about how metastatic cancer cells adapt to and colonize new tissue environments.Here,using clinical samples,patient-derived xenograft(PDX)samples,PDX cells,and primary/metastatic cell lines,we discovered that liver metastatic colorectal cancer(CRC)cells lose their colon-specific gene transcription program yet gain a liver-specific gene transcription program.We showed that this transcription reprogramming is driven by a reshaped epigenetic landscape of both typical enhancers and super-enhancers.Further,we identified that the liver-specific transcription factors FOXA2 and HNF1A can bind to the gained enhancers and activate the liver-specific gene transcription,thereby driving CRC liver metastasis.Importantly,similar transcription reprogramming can be observed in multiple cancer types.Our data suggest that reprogrammed tissue-specific transcription promotes metastasis and should be targeted therapeutically. | Shuaishuai Teng Yang Eric Li Ming Yang Rui Qi Yiming Huang Qianyu Wang Yanmei Zhang Shanwen Chen Shasha Li Kequan Lin Yang Cao Qunsheng Ji Qingyang Gu Yujing Cheng Zai Chang Wei Guo Pengyuan Wang Ivan Garcia-Bassets Zhi John Lu Dong Wang | 2020 | Cell Research2020，30，1： | 11 |
| 4 | Solar flare forecasting using learning vector quantity and unsupervised clustering techniques显示文摘In this paper, a combined method of unsupervised clustering and learning vector quantity (LVQ) is presented to forecast the occurrence of solar flare. Three magnetic parameters including the maximum horizontal gradient, the length of the neutral line, and the number of singular points are extracted from SOHO/MDI longitudinal magnetograms as measures. Based on these pa- rameters, the sliding-window method is used to form the sequential data by adding three days evolutionary information. Con- sidering the imbalanced problem in dataset, the K-means clustering, as an unsupervised clustering algorithm, is used to convert imbalanced data to balanced ones. Finally, the learning vector quantity is employed to predict the flares level within 48 hours. Experimental results indicate that the performance of the proposed flare forecasting model with sequential data is improved. | LI Rong WANG HuaNing CUI YanMei HUANG Xin | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，8： | 10 |
| 5 | One-step synthesis of cobalt,nitrogen-codoped carbon as nonprecious bifunctional electrocatalyst for oxygen reduction and evolution reactions显示文摘It is highly desired for the development of efficient bifunctional electrocatalyst for both oxygen reduction reaction(ORR) and oxygen evolution reaction(OER) in renewable energy technologies. In this work,cobalt, nitrogen-codoped carbon was prepared by a facile one-step method and demonstrated to exhibit good electrocatalytic performance for ORR and OER via a complete four-electron process. Besides, the catalyst prepared at900 °C also displayed excellent stability for both ORR and OER. Furthermore, the origin of catalytic activity was also explored, which was attributed to the synergistic effects of metallic Co and quaternary N. | Sijie Guo Yanmei Yang Naiyun Liu Shi Qiao Hui Huang Yang Liu Zhenhui Kang | 2016 | Science Bulletin2016，61，1： | 7 |
| 6 | Super-resolution fluorescence-assisted diffraction computational tomography reveals the threedimensional landscape of the cellular organelle interactome显示文摘The emergence of super-resolution(SR)fluorescence microscopy has rejuvenated the search for new cellular substructures.However,SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment.Thus,we developed SR fluorescence-assisted diffraction computational tomography(SR-FACT),which combines label-free three-dimensional optical diffraction tomography(ODT)with two-dimensional fluorescence Hessian structured illumination microscopy.The ODT module is capable of resolving the mitochondria,lipid droplets,the nuclear membrane,chromosomes,the tubular endoplasmic reticulum,and lysosomes.Using dual-mode correlated live-cell imaging for a prolonged period of time,we observed novel subcellular structures named dark-vacuole bodies,the majority of which originate from densely populated perinuclear regions,and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma membrane.This work demonstrates the unique capabilities of SR-FACT,which suggests its wide applicability in cell biology in general. | Dashan Dong Xiaoshuai Huang Liuju Li Heng Mao Yanquan Mo Guangyi Zhang Zhe Zhang Jiayu Shen Wei Liu Zeming Wu Guanghui Liu Yanmei Liu Hong Yang Qihuang Gong Kebin Shi Liangyi Chen | 2020 | Light(Science & Applications)2020，9，1： | 7 |
| 7 | Identification of differentially expressed proteins in poplar leaves in-duced by Marssonina brunnea f. sp. Multigermtubi显示文摘Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone 'NL895' (P. euramericana CL'NL895'), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. mul- tigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Ma- trix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and un- classified proteins. | Kun Yuan Bo Zhang Yanmei Zhang Qiang Cheng Mingxiu Wang Minren Huang | 2008 | Journal of Genetics and Genomics2008，35，1： | 6 |
| 8 | Curative effect of assisted reproduction technology by Traditional Chinese Medicine multi-channel interventional therapy on 95 cases of in vitro fertilization and embryo transfer failure显示文摘OBJECTIVE: To evaluate the curative effect of Traditional Chinese Medicine(TCM) multi-channel interventional therapy on women with Assisted Reproductive Technology(ART) failure, to compare the curative effect of the dual therapy and triple therapy on women with ART Failure, and to choose the best TCM interventional therapeutic plan.METHODS: The 95 cases with ART Failure from West China second University Hospital of Sichuan University meeting the inclusion criteria were randomly divided into three groups:dual therapy group(31 cases), triple therapy group(33 cases)and control group(31 cases). According to the intervene treatment of in vitro Fertilization and Embryo Transfer(IVF-ET) long cycle scheme, the control group wait naturally for 3 months before IVFET. The dual therapy group take TCM prescriptionⅡ of cultivating emotion and assisting reproduction and auricular acupoint therapy for 3 months before IVF-ET, then Western Medicine treatment progestin supporting as well as auricular application and Antai Recipe after IVF-ET transplantation.The triple therapy group take TCM prescription Ⅱof cultivated emotion and assisted reproduction,auricular acupoint therapy and retention enema of TCM, and combination treatment the same as dual therapy group after transplantation. The natural pregnancy number, the period condition of secondary IVF-ET and the improvement of the kidney deficiency, liver depression and blood stasis syndrome among those three groups were compared.RESULTS: It was showed from analysis in 95 cases with ART failure that the number of natural preg-nancy was as followings: 3 patients from the dual therapy group, 10 patients from the triple therapy group, and no patient from the control group. The comparison among three groups have statistical significance(P < 0.05). The treatment group is superior to the control group, while the triple therapy is superior to the dual therapy. The comparison of the condition of the fertility rate, clinical pregnancy rate, biochemical pregnancy rate and early abortion rate during the period of secondary IVF-ET between pre-therapy and post-treatment of both the dual therapy group and the triple therapy group have statistical significance(P < 0.05). The comparison of the improvement of the kidney deficiency,liver depression and blood stasis syndrome between pre-therapy and post-treatment of both the dual therapy group and the triple therapy group have statistical significance(P < 0.05). The comparison between three groups after treatment have statistical significance(P < 0.05).CONCLUSION: TCM multi-channel interventional therapy can increase the natural pregnancy rate of patients with ART Failure(the triple therapy is superior to the dual therapy); it can increase the fertility rate, clinical pregnancy rate, and decrease the early abortion rate during the period of secondary IVF-ET; it can improve syndromes of kidney deficiency, liver depression and blood stasis. | Xia Wanting Zhong Yanmei Zhang Ying Xue Huarong Huang Jinzhu Wang Zhen Zhou Hang Ma Qianhong Zeng Qian | 2017 | Journal of Traditional Chinese Medicine2017，37，5： | 4 |
| 9 | An improved Landsat Image Mosaic of Antarctica显示文摘A revised Landsat Image Mosaic of Antarctica (LIMA) is presented, using the 1073 multi-band scenes of the original Landsat-7 ETM+ LIMA image collection available at the United States Geological Survey (USGS: http://gffzza64998f73d704ee0h6op60buvqwfu60f9.ffgz.tsg.suse.edu.cn/). Three improvements have been applied during the data processing: (1) DN saturation is adjusted by adopting a linear regression, which has a lower root mean square error than the ratio regression used by LIMA; (2) solar elevation angle is calculated using pixel-level latitude/longitude and the acquisition time and date of the central pixel of the scene, improving slightly upon the bilinear interpolation of the solar elevation angles of scene corners applied in LIMA; and (3) two additional image bands, Band 5 and Band 7, are sharpened using the panchromatic band (Band 8) and a Gram-Schmidt Spectral Sharpening algorithm to more easily distinguish snow, cloud and exposed rocks. The final planetary reflectance product is stored in 16-bit bands to preserve the full radiometric content of the scenes. A comparative statistical analysis among 12 sample regions indicates that the new mosaic has enhanced visual qualities, information entropy, and information content for land cover classification relative to LIMA. | HUI FengMing CHENG Xiao LIU Yan ZHANG YanMei YE YuFang WANG XianWei LI Zhan WANG Kun ZHAN ZhiFei GUO JianHong HUANG HuaBing LI XiuHong GUO ZiQi GONG Peng | 2013 | Science China Earth Sciences2013，56，1： | 4 |
| 10 | Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells显示文摘Background:Tooth is vital not only for a good smile,but also good health.Yet,we lose tooth regularly due to accidents or diseases.An ideal solution to this problem is to regenerate tooth with patients’own cells.Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental mesenchymes.Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 hESC.We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human tooth.Conclusion:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. | Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei | 2013 | Cell Regeneration2013，2，1： | 4 |
| 11 | A putative bHLH transcription factor is a candidate gene for male sterile 32,a locus affecting pollen and tapetum development in tomato显示文摘The tomato(Solanum lycopersicum)male sterile 32(ms32)mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas.In this study,histological examination of anthers revealed dysfunctional pollen and tapetum development in the ms32 mutant.The ms32 locus was fine mapped to a 28.5 kb interval that encoded four putative genes.Solyc01g081100,a homolog of Arabidopsis bHLH10/89/90 and rice EAT1,was proposed to be the candidate gene of MS32 because it contained a single nucleotide polymorphism(SNP)that led to the formation of a premature stop codon.A codominant derived cleaved amplified polymorphic sequence(dCAPS)marker,MS32D,was developed based on the SNP.Real-time quantitative reverse-transcription PCR showed that most of the genes,which were proposed to be involved in pollen and tapetum development in tomato,were downregulated in the ms32 mutant.These findings may aid in marker-assisted selection of ms32 in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato. | Xiaoyan Liu Mengxia Yang Xiaolin Liu Kai Wei Xue Cao Xiaotian Wang Xiaoxuan Wang Yanmei Guo Yongchen Du Junming Li Lei Liu Jinshuai Shu Yong Qin Zejun Huang | 2019 | Horticulture Research2019，6，1： | 3 |
| 12 | Overview on the recent study of antimicrobial peptides: Origins, functions, relative mechanisms and application显示文摘 | Yanmei Li Qi Xiang Qihao Zhang Yadong Huang Zhijian Su | 2012 | Peptides2012，，2： | 3 |
| 13 | Advanced surface engineering of titanium materials for biomedical applications:From static modification to dynamic responsive regulation显示文摘Titanium(Ti)and its alloys have been widely used as orthopedic implants,because of their favorable mechanical properties,corrosion resistance and biocompatibility.Despite their significant success in various clinical applications,the probability of failure,degradation and revision is undesirably high,especially for the patients with low bone density,insufficient quantity of bone or osteoporosis,which renders the studies on surface modification of Ti still active to further improve clinical results.It is discerned that surface physicochemical properties directly influence and even control the dynamic interaction that subsequently determines the success or rejection of orthopedic implants.Therefore,it is crucial to endow bulk materials with specific surface properties of high bioactivity that can be performed by surface modification to realize the osseointegration.This article first reviews surface characteristics of Ti materials and various conventional surface modification techniques involving mechanical,physical and chemical treatments based on the formation mechanism of the modified coatings.Such conventional methods are able to improve bioactivity of Ti implants,but the surfaces with static state cannot respond to the dynamic biological cascades from the living cells and tissues.Hence,beyond traditional static design,dynamic responsive avenues are then emerging.The dynamic stimuli sources for surface functionalization can originate from environmental triggers or physiological triggers.In short,this review surveys recent developments in the surface engineering of Ti materials,with a specific emphasis on advances in static to dynamic functionality,which provides perspectives for improving bioactivity and biocompatibility of Ti implants. | Pinliang Jiang Yanmei Zhang Ren Hu Bin Shi Lihai Zhang Qiaoling Huang Yun Yang Peifu Tang Changjian Lin | 2023 | Bioactive Materials2023，，9： | 2 |
| 14 | Modeling interannual variability of global soil respiration from climate and soil properties显示文摘 | Shutao Chen Yao Huang Jianwen Zou Qirong Shen Zhenghua Hu Yanmei Qin Haishan Chen Genxing Pan | 2010 | Agricultural and Forest Meteorology2010，，4： | 2 |
| 15 | Synthesis and photochemical properties of La-doped HGa2Nb3O10显示文摘 | Huang Yunfang Xie Yanmei Fan Leqing | 2008 | Hydrogen Energy2008，33，： | 1 |
| 16 | Upregulation of angiotensin-converting enzyme 2 by all-trans retinoic acid in spontaneously hypertensive rats 显示文摘 | Zhong Jiuchang Huang Dongyang Yang Yanmei | 2004 | Hypertension2004，44，6： | 1 |
| 17 | Short-Term Solar Flare Prediction Using a Sequential Supervised Learning Method显示文摘 | Daren Yu Xin Huang Huaning Wang Yanmei Cui | 2009 | Solar Physics2009，，1： | 1 |
| 18 | An SVM-Based Prediction Method for Solving SAT Problems显示文摘We show how Support vector machines(SVM) can be applied to the Satisfiability(SAT) problem and how their prediction results can be naturally applied to both incomplete and complete SAT solvers. SVM is used for the classification of the variables in the SAT problem and the classification results are the assignment of the variables. And we also present empirical results of applying SVM to instances of the SAT problem from the Center for Discrete Mathematics and Theoretical Computer Science(DIMACS) archive and compare them against the results of other incomplete and complete algorithms for the SAT problem. | HUANG Shaobin LI Ya LI Yanmei | 2019 | Chinese Journal of Electronics2019，28，2： | 1 |
| 19 | Determination of 27 chemical constituents in Chinese southwest tobacco by FT-NIR spectroscopy显示文摘 | Jia Duan Yue Huang Zuhong Li Bo Zheng Qianqian Li Yanmei Xiong Lijun Wu Shungeng Min | 2012 | Industrial Crops & Products2012，，： | 1 |
| 20 | Antioxidant Activity of Different Solvent Extracts of Blumea Megacephala in Vitro显示文摘[Objectives] The aim was to study the antioxidant activity of different solvent extracts of Blumea Megacephala in vitro. [Methods] The removal rates of DPPH free radicals and ABTS free radicals and the reduction capacity of Fe^(3+) by ethanol extract( EE),acetone extract( AE) and ethyl acetate extract( EAE) of Blumea Megacephala were detected. [Results]The three extracts of Blumea Megacephala had certain antioxidant activity,of which the antioxidant activity of ethyl acetate extract was the strongest and has great potential for development.[Conclusions]The study can provide scientific reference for the deep research on the antioxidant activity of Blumea Megacephala. | Xinger ZHOU Yanmei CHEN Meili WEI Guixiang HUANG Suoyi HUANG Haifeng LU | 2018 | Medicinal Plant2018，9，4： | 1 |
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