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| 1 | Establishment of a transgenic mouse model with liver-specific expression of secretory immunoglobulin D显示文摘Mutation of mevalonate kinase(MVK) is thought to account for most cases of hyperimmunoglobulinemia D syndrome(HIDS) with recurrent fever.However,its mechanism and the relationship between elevated serum immunoglobulin D(IgD) and the clinical features of HIDS are unclear.In this study,we generated by fusion PCR a vector to express high levels of chimeric secretory IgD(csIgD) specifically in the liver.We then generated seven founder lines of transgenic mice by co-microinjection,and verified them using genomic PCR and Southern blotting.We detected the expression of csIgD by reverse transcription PCR,quantitative PCR,western blotting,and enzyme-linked immunosorbent assays.We demonstrated that csIgD could be specifically and stably expressed in the liver.We used flow cytometry to show that overexpression of csIgD in the bone marrow and spleen cells had no effect on B cell development.Morphologic and anatomical observation of the transgenic mice revealed skin damage,hepatosplenomegaly,and nephromegaly in some transgenic mice;in these mice,pathological sections showed high levels of cell necrosis and protein-like sediments in the liver,spleen,and kidney.We demonstrated that the genomic insertion sites of the transgenes did not disrupt the MVK gene on mouse chromosome 5.This transgenic mouse will be useful to explore the pathogenesis of HIDS. | WANG Ping WEI ZhiGuo YAN BoWen HUANG Tan GOU KeMian DAI YunPing ZHENG Min WANG MeiLi CHENG XueQian WANG XiFeng XU Chen SUN Yi | 2012 | Science China(Life Sciences)2012，55，3： | 6 |
| 2 | Genome-wide detection of additional fetal chromosomal abnormalities by cell-free DNA testing of 15,626 consecutive pregnant women显示文摘Cell-free DNA(cfDNA) testing for common fetal trisomies(T21, T18, T13) is highly effective. However, the usefulness of cfDNA testing in detecting other chromosomal abnormalities is unclear. We evaluated the performance of cfDNA testing for genome-wide abnormalities, and analyzed the incremental yield by reporting extra abnormalities. We performed genome-wide cfDNA testing in 15,626 consecutive pregnancies prospectively enrolled in this study. cfDNA testing results were reported and counseling was given depending on the presence of extra chromosomal abnormalities. cfDNA testing identified 190 cases(1.2%)of chromosomal abnormalities including 100 common trisomies and 90 additional abnormalities. By expanding the cfDNA reporting range to genome-wide abnormalities, the false positive rate increased to 0.39%(P<0.001) and positive predictive value(PPV) was reduced to 65.58%(P=0.42). However, the detection yield increased from 0.44% to 0.65%(P=0.014), and cfDNA testing detected 38.61%(39/101) additional abnormalities with no ultrasound and biochemical screening findings. cfDNA testing outperformed biochemical screening by showing 60 times higher true positive rate and fewer false negative results.Genome-wide cfDNA testing significantly increased the diagnostic yield by detecting extra abnormalities, especially those without diagnostic indications. Genome-wide cfDNA testing has fewer false positive and false negative results compared with biochemical screening. | Hong Yao Ya Gao Jia Zhao Rong Zhang Huixin Xu Huamei Hu Yanmei Luo Yuying Yuan Meili Fu Hongyun Zhang Hui Jiang Wei Wang Huanming Yang Jian Wang Zhiqing Liang Fang Chen | 2019 | Science China(Life Sciences)2019，62，2： | 4 |
| 3 | Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury显示文摘Compelling evidence has revealed that biased activation of G protein-coupled receptor(GPCR)signaling,including angiotensin II(AngII)receptor type 1(AT1)signaling,plays pivotal roles in vascular homeostasis and injury,but whether a clinically relevant endogenous biased antagonism of AT1 signaling exists under physiological and pathophysiological conditions has not been clearly elucidated.Here,we show that an extracellular matrix protein,cartilage oligomeric matrix protein(COMP),acts as an endogenous allosteric biased modulator of the AT1 receptor and its deficiency is clinically associated with abdominal aortic aneurysm(AAA)development.COMP directly interacts with the extracellular N-terminus of the AT1 via its EGF domain and inhibits AT1-β-arrestin-2 signaling,but not Gq or Gi signaling,in a selective manner through allosteric regulation of AT1 intracellular conformational states.COMP deficiency results in activation of AT1a-β-arrestin-2 signaling and subsequent exclusive AAA formation in response to AngII infusion.AAAs in COMP–/–or ApoE–/–mice are rescued by AT1a orβ-arrestin-2 deficiency,or the application of a peptidomimetic mimicking the AT1-binding motif of COMP.Explorations of the endogenous biased antagonism of AT1 receptor or other GPCRs may reveal novel therapeutic strategies for cardiovascular diseases. | Yi Fu Yaqian Huang Zhao Yang Yufei Chen Jingang Zheng Chenfeng Mao Zhiqing Li Zhixin Liu Bing Yu Tuoyi Li Meili Wang Chanjuan Xu Yiwei Zhou Guizhen Zhao Yiting Jia Wei Guo Xin Jia Tao Zhang Li Li Ziyi Liu Shengchao Guo Mingliang Ma Heng Zhang Bo Liu Junbao Du Wengong Wang Chaoshu Tang Pei Gao Qingbo Xu Xian Wang Jianfeng Liu Jinpeng Sun Wei Kong | 2021 | Cell Research2021，31，7： | 2 |
| 4 | Prognostic factors in patients with stage IV non-small cell lung cancer显示文摘Objective: To investigate the prognostic factors for stage IV non-small cell lung cancer (NSCLC) with distant metas- tasis and establish a reliable model of clinical prognostic index. Methods: From January 1990 to April 2005, 313 primary NSCLC patients with metastasis, who had been treated in Shanghai Chest Hospital, were reviewed. Survival time was estimated accord- ing to the Kaplan-Meier method. Cox proportional hazard regression model was used for multivariate analysis. Results: Among the 313 cases of non-small cell lung cancer (NSCLC) at stage IV, there were 218 and 95 patients with metastasis to single and different organs, respectively. The overall median survival time for all 313 cases of NSCLC patients was 10.8 (9.00, 12.30) months and the overall 1-, 2-, 3-, 4- and 5-year survival rate was 45%, 18%, 12%, 4% and 0%. There were 63, 174, 127, 36, 18, 11 and 5 patients with metastasis to brain (20.13%), bone (55.59%), lung (40.58%), liver (11.50%), adrenal gland (5.75%), subcutaneous (3.51%) and others, respectively. The survival time was shortest in subcutaneous metastasis (4.6 months), and liver 7.0 months, brain 8.0 months, adrenal gland 8.6 months, bone 10.6 months, lung 11.8 months. Kaplan-Meier estimation showed that patients anatomic typing, KPS, numbers of organ with metastasis, appetite, liver, adrenal gland and subcutane- ous metastasis, body weight loss, smoking, index of smoking, chemotherapy, cycles of chemotherapy were the predictors of survival. Multivariate analysis showed survival statistically significant correlation with anatomic typing, KPS, appetite, liver and subcutaneous metastasis, body weight loss, cycles of chemotherapy. The relative risk (RR) was 1.51, 1.97, 1.55, 1.67, 2.56, and 2.56 respectively. Conclusion: Survival time decreases distinctly in patients who had distant metastasis to more than two different organs (P<0.01). Bone is the commonest organ for distant metastasis in lung cancer. The prognosis is poor when lung cancer appears subcutaneous metastasis and liver metastasis. Independent prognostic factors in patient with stage IV non-small cell lung cancer were liver and subcutaneous metastasis, anatomic typing, KPS, appetite, body weight loss, cycles of chemotherapy. | Meili Ma Jie Shen Liyan Jiang Baohui Han Hao Bai Hao Ji Yizuo Zhao Bo Jin Yongfeng Yu Jun Pei Wei Zhang | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，5： | 1 |
| 5 | Antioxidant Activity of Different Solvent Extracts of Blumea Megacephala in Vitro显示文摘[Objectives] The aim was to study the antioxidant activity of different solvent extracts of Blumea Megacephala in vitro. [Methods] The removal rates of DPPH free radicals and ABTS free radicals and the reduction capacity of Fe^(3+) by ethanol extract( EE),acetone extract( AE) and ethyl acetate extract( EAE) of Blumea Megacephala were detected. [Results]The three extracts of Blumea Megacephala had certain antioxidant activity,of which the antioxidant activity of ethyl acetate extract was the strongest and has great potential for development.[Conclusions]The study can provide scientific reference for the deep research on the antioxidant activity of Blumea Megacephala. | Xinger ZHOU Yanmei CHEN Meili WEI Guixiang HUANG Suoyi HUANG Haifeng LU | 2018 | Medicinal Plant2018，9，4： | 1 |
| 6 | Sufentanil and fentanyl for pediatric cardiac surgical anesthetic effect comparison 显示文摘 | Bin Yong Qin Meili Li Wei | 2010 | Journal of2010，2010，15： | 1 |
| 7 | Development and application of a method for analysis of phthalates in ham sausages by solid-phase extraction and gas chromatography–mass spectrometry显示文摘 | Zhiyong Guo Sui Wang Danyi Wei Meili Wang Huina Zhang Panpan Gai Jing Duan | 2009 | Meat Science2009，，3： | 1 |
| 8 | Constitutive equation for elevated temperature flow behavior of TiNiNb alloy based on orthogonal analysis显示文摘 | Meili Xiao Fuguo Li Wei Zhao Genlin Yang | 2011 | Materials and Design2011，，： | 1 |
| 9 | New Insights into Nested Long Terminal Repeat Retrotransposons in Brassica Species显示文摘长终端重复(LTR ) retrotransposons, transposons 的最前的类型之一,不断地改变或修改并且通过戏剧的增长的爆炸重新组织染色体基因功能。许多 LTR-TEs 优先地在另外的 LTR-TEs,而是原因和这些嵌套的 LTR-TEs 的进化意义以内插入很好没被理解。在这研究,包含 2020 个细菌的人工的染色体(用绳子拖的平底渡船) 的 Brassica 顺序的 1.52Gb 的一个总数被扫描,并且六个细菌的人工的染色体(用绳子拖的平底渡船) 与极其嵌套的 LTR-TEs 克隆(LTR-TEs 密度:7.24/kb ) 为进一步的分析被选择。在六条用绳子拖的平底渡船中的四个的 LTR-TEs 的多数被发现从在 BAC 区域以内发源的 retrotransposons 的快速的增长被发源,与仅仅从从在用绳子拖的平底渡船区域外面的 retrotransposons 的增长和插入发源的一些 LTR-TEs 近似 523Mya。LTR-TEs 最好也插入了到 TA 富有的重复区域。由 Genescan 的基因预言在全部的用绳子拖的平底渡船序列的 0.84Mb 识别了 207 基因。仅仅一些基因(3/207 ) 能被匹配到 Brassica 表示顺序标签(EST ) 数据库,显示大多数基因在 retrotransposon 以后是不活跃的插入。六条用绳子拖的平底渡船中的五个是通常认为地 centromeric。因此,在着丝点区域嵌套 LTR-TEs 很快被复制,反复插入了,并且压制基因的活动并且改组的行为 centromeric 序列的结构。我们的结果建议 LTR-TEs 爆炸了并且在本地规模上增殖创造嵌套的 LTR-TE 区域,和那这些嵌套的 LTR-TEs 戏在着丝点的形成的一个角色。 | Lijuan Wei Meili Xiaoa Zeshan An Bi Ma Annaliese S. Mason Wei Qian Jiana Li Donghui Fu | 2013 | Molecular Plant2013，6，2： | 0 |
| 10 | Statistical Analysis of the Hole Primary Halo in Dachang Sn-Zn Polymetallic Mining Area of Guangxi显示文摘The complexity and diversity of Ore forming is the commonness,of which the difficulty is the evolutional characteristics of geochemical elements.The metallogetic process can be speculated and get useful and microcosmic message for next exploration.In this paper,the research of cluster analysis and factor analysis of the hole primary halo in Dachang Sn-Zn polymetallic mining area in Guangxi reveal that metallogenic process is very complex and superposed by mineralization.It is considered that the enrichment of Zn,Sn superposed anaphase.By sulphide polymetallic,Au is very independency and that Sb,Pb,Ag and Cu are proximal indicator elements for exploring Zn,Sn in the mine. | Yang Xiaokun Qin Dexian Wu Wei Feng Meili | 2008 | Engineering Sciences2008，6，1： | 0 |
| 11 | Towards uniform point distribution in feature-preserving point cloud filtering显示文摘While a popular representation of 3D data,point clouds may contain noise and need filtering before use.Existing point cloud filtering methods either cannot preserve sharp features or result in uneven point distributions in the filtered output.To address this problem,this paper introduces a point cloud filtering method that considers both point distribution and feature preservation during filtering.The key idea is to incorporate a repulsion term with a data term in energy minimization.The repulsion term is responsible for the point distribution,while the data term aims to approximate the noisy surfaces while preserving geometric features.This method is capable of handling models with fine-scale features and sharp features.Extensive experiments show that our method quickly yields good results with relatively uniform point distribution. | Shuaijun Chen Jinxi Wang Wei Pan Shang Gao Meili Wang Xuequan Lu | 2023 | Computational Visual Media2023，9，2： | 0 |
| 12 | Molecular Properties of the Epstein-Barr virus BFRF3 Gene显示文摘Dear Editor,We report the results of cloning,expression,subcellular localization analysis,and molecular properties of the Epstein-Barr virus (EBV) BFRF3 gene,using several bioinformatics tools.Bioinformatics analysis indicated that the encoding protein of EBV BFRF3 gene (designated BFRF3) has a conserved Herpes_capsid domain,which was found to be closely related to the gammaherpesvirus capsid protein family,and is highly conserved among its counterparts.Multiple nucleic acid sequence and amino acid (aa) sequence alignments suggested that BFRF3 has a relatively higher | Mingsheng Cai Zhiyao Zhao Wei Cui Lin Yang Junyi Zhu Yalan Chen Changling Ma Zhuqing Yuan Meili Li | 2013 | Virologica Sinica2013，28，6： | 0 |
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