|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis显示文摘 | Xu Zhao Ying Yang Bao-Fa Sun Yue Shi Xin Yang Wen Xiao Ya-Juan Hao Xiao-Li Ping Yu-Sheng Chen Wen-Jia Wang Kang-Xuan Jin Xing Wang Chun-Min Huang Yu Fu Xiao-Meng Ge Shu-Hui Song Hyun Seok Jeong Hiroyuki Yanagisawa Yamei Niu Gui-Fang Jia Wei Wu Wei-Min Tong Akimitsu Okamoto Chuan He Jannie M Rendtlew Danielsen Xiu-Jie Wang Yun-Gui Yang | 2014 | Cell Research2014，24，12： | 109 |
| 2 | Energy balance closure at ChinaFLUX sites显示文摘Network of eddy covariance observation is measuring long-term carbon and water fluxes in contrasting ecosystems and climates. As one important reference of independently evaluating scalar flux estimates from eddy covariance, energy balance closure is used widely in study of carbon and water fluxes. Energy balance closure in ChinaFLUX was evaluated by statistical regression of turbulent energy fluxes (sensible and latent heat) against available energy (net radiation, soil heat flux, canopy heat storage) and the energy balance ratio (EBR) and the frequency distribution of relative errors of energy balance (δ). The trends of diurnal and seasonal variation of energy balance in ChinaFLUX were analyzed. The results indicated that the imbalance was prevalent in all observation sites, but there were little differences among sites because of the properties variation of sites. The imbalance was greater during nocturnal periods than daytime and closure was improved with friction velocity intensifying. Generally the results suggested that estimates of the scalar turbulent fluxes of sensible and latent heat were underestimated and/or that available energy was overestimated. Finally, we discussed certain factors that are contributed to the imbalance of energy, such as systematic errors associated with the sampling mismatch, systematic instrument bias, neglected energy sinks, low and high frequency loss of turbulent fluxes and advection of heat and water vapor. | LI Zhengquan, YU Guirui, WEN Xuefa, ZHANG Leiming, REN Chuanyou & FU Yuling Chinese Ecosystem Research Network Synthesis Research Center, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Science China Earth Sciences2005，48，z1： | 50 |
| 3 | A new cytotoxic 2-(2-phenylethyl)chromone from Chinese eaglewood显示文摘新复合 8-chloro-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl )-5,6,7,8-tetrahydro-4H-chromen-4-one (1 ) 从中国沉香被孤立[Aquilaria sinensis (愁相) Gilg ] 。它的结构被阐明根据光谱数据。与 14.6 渭 g /mL 的 IC50 价值由 MTT 方法在 vitro 对人的胃的癌症房间线(SGC-7901 ) 加重 1 显示出的 cytotoxicity。 | Jun Liu Jiao Wu You Xing Zhao Yuan Yuan Deng Wen Li Mei Hao Fu Dai | 2008 | Chinese Chemical Letters2008，19，8： | 25 |
| 4 | Development of Intensified Technologies of Vanadium-Bearing Titanomagnetite Smelting显示文摘It was very difficult for the smelting of vanadium-bearing titanomagnetite by blast furnace because the content of TiO2 of blast furnace slag could amount to 20%-25%.After long term development and continuous improvement,special intensified smelting technologies for vanadium-bearing titanomagnetite by blast furnace were obtained and improved gradually.With the improvement of beneficiated material level and equipment level,smelting intensity has been increased gradually and the highest comprehensive smelting intensity reached 1.45 t/(m3·d).Technical-economic indexes of blast furnace have also been increased remarkably.The highest utilization coefficient exceeded 2.7 t/(m3·d)on the condition that the burden grade was only about 50%. | FU Wei-guo WEN Yong-cai XIE Hong-en | 2011 | Journal of Iron and Steel Research(International)2011，18，4： | 26 |
| 5 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 6 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 7 | TGF-β promotes invasion and metastasis of gastric cancer cells by increasing fascinl expression via ERK and JNK signal pathways显示文摘 | Hua Fu Zhongliang Hu Jifang Wen Kuansong Wang Ying Liu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 19 |
| 8 | Wheat, maize and sunflower cropping systems selectively influence bacteria community structure and diversity in their and succeeding crop's rhizosphere显示文摘Wheat and maize are increasingly used as alternative crops to sunflower monocultures that dominate the Hetao Irrigation District in China.Shifts from sunflower monocultures to alternate cropping systems may have significant effects on belowground microbial communities which control nutrient cycling and influence plant productivity.In this research,rhizosphere bacterial communities were compared among sunflower,wheat and maize cropping systems by 454 pyrosequencing.These cropping systems included 2 years wheat(cultivar Yongliang 4) and maize(cultivar Sidan 19) monoculture,more than 20 years sunflower(cultivar 5009) monoculture,and wheat-sunflower and maize-sunflower rotation.In addition,we investigated rhizosphere bacterial communities of healthy and diseased plants at maturity to determine the relationship between plant health and rotation effect.The results revealed taxonomic information about the overall bacterial community.And significant differences in bacterial community structure were detected among these cropping systems.Eight of the most abundant groups including Proteobacteria,Bacteroidetes,Acidobacteria,Gemmatimonadetes,Chloroflexi,Actinobacteria,Planctomycetes and Firmicutes accounted for more than 85%of the sequences in each treatment.The wheat-wheat rhizosphere had the highest proportion of Acidobacteria,Bacteroidetes and the lowest proportion of unclassified bacteria.Wheat-sunflower cropping system showed more abundant Acidobacteria than maize-sunflower and sunflower monoculture,exhibiting some influences of wheat on the succeeding crop.Maize-maize rhizosphere had the highest proportion of γ-Proteobacteria,Pseudomonadales and the lowest proportion of Acidobacteria.Sunflower rotation with wheat and maize could increase the relative abundance of the Acidobacteria while decrease the relative abundance of the unclassified phyla,as was similar with the health plants.This suggests some positive impacts of rotation with wheat and maize on the bacterial communities within a single field.These results demonstrate that different crop rotation systems can have significant effects on rhizosphere microbiomes that potentially alter plant productivities in agricultural systems. | WEN Xin-ya Eric Dubinsky WU Yao YU Rong CHEN Fu | 2016 | Journal of Integrative Agriculture2016，15，8： | 18 |
| 9 | Research Progress on the Relationship between Coronary Artery Calcification and Chronic Renal Failure显示文摘 | Jun Lai Gael Akindavyi Qiang Fu Zhi-Liang Li Hui-Min Wang Li-Hua Wen | 2018 | Chinese Medical Journal2018，，5： | 18 |
| 10 | Mushroom Poisoning Outbreaks—China,2010–2020显示文摘ABSTRACT Introduction:Mushroom poisoning was the leading cause of foodborne disease outbreaks and outbreak-associated deaths in China.Mushroom poisoning outbreak surveillance can provide insight into the epidemiological characteristics of mushroom poisonings and guide policymaking and health education to reduce illnesses and deaths.Methods:Foodborne Disease Outbreak Surveillance System was upgraded in 2011 to collect foodborne disease outbreaks in China.Mushroom poisoning outbreaks during 2010–2020 were selected to analyze geographical distribution,seasonal distribution,and setting of food preparation.Results:A total of 10,036 outbreaks,which resulted in 38,676 illnesses and 788 deaths,were reported in this period.Mushroom poisonings occurred all over the country,but with highest incidence in the southwest and central China. | Weiwei Li Sara M.Pires Zhitao Liu Jinjun Liang Yafang Wang Wen Chen Chengwei Liu Jikai Liu Haihong Han Ping Fu Yunchang Guo | 2021 | China CDC weekly2021，3，24： | 18 |
| 11 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 12 | Oral berberine improves brain dopa/dopamine levels to ameliorate Parkinson’s disease by regulating gut microbiota显示文摘The phenylalanine-tyrosine-dopa-dopamine pathway provides dopamine to the brain.Iin this process,tyrosine hydroxylase(TH)isthe rate-limiting enzyme that hydroxylates tyrosine and generates levodopa(L-dopa)with tetranydrobiopterin(BH_(4))as a coenzyme.Here,we show that oral berberine(BBR)might supply H^(·) through dihydroberberine(reduced BBR produced by bacterial nitroreductase)and promote the production of BHl from dihydrobiopterin;the increased BH,enhances TH activity,which accelerates the production of L-dopa by the gut bacteria.Oral BBR acts in a way similar to vitamins.The L-dopa produced by theintestinal bacteria enters the brain through the circulation and is transformed to dopamine.To verify the gut-brain dialog activatedby BBR's effect,Enterococcus foecalis or Enterococcus faecium was transplanted into Parkinson's disease(PD)mice.The bacteriasignificantly increased brain dopamine and ameliorated PD manifestation in mice;additionally,combination of BBR with bacteriashowed better therapeutic effect than that with bacteria alone.Moreover,2,4,6-trimethy-pyranylium tetrafluoroborate(TMP-TFB)-derivatized matrix-assisted laser desorption mass spectrometry(MALDI-MS)imaging of dopamine identihed elevated striataldopamine levels in mouse brains with oral Enterococcus,and BBR strengthened the imaging intensity of brain dopamine.Theseresults demonstrated that BBR was an agonist of TH in Enterococcus and could lead to the production of L-dopa in the gut.Furthermore,a study of 28 patients with hyperlipidemia conhrmed that oral BBR increased bloodfecal L-dopa by the intestinalbacteria.Hence,BBR might improve the brain function by upregulating the biosynthesis of-dopa in the gut microbiota through avitamin-like effect. | Yan Wang Qian Tong Shu-Rong Ma Zhen-Xiong Zhao Li-Bin Pan Lin Cong Pei Han Ran Peng Hang Yu Yuan Lin Tian-Le Gao Jia-Wen Shou Xiao-Yang Li Xian-Feng Zhang Zheng-Wei Zhang Jie Fu Bao-Ying Wen Jin-Bo Yu Xuetao Cao Jian-Dong Jiang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 16 |
| 13 | Initial study of biexponential model of intravoxel incoherent motion magnetic resonance imaging in evaluation of the liver fibrosis显示文摘 | Chen Cuiyun Wang Bin Shi Dapeng Fu Fangfang Zhang Jiliang Wen Zejun Zhu Shaocheng Xu Junling Lin Qing Li Jing Dou Shewei | 2014 | Chinese Medical Journal2014，，17： | 15 |
| 14 | Associations of ABCB1, NFKB1, CYP3A, and NR112 polymorphisms with cyclosporine trough concentrations in Chinese renal transplant recipients显示文摘 | Yu ZHANG Jia-li LI Qian FU Xue-ding WANG Long-shan LIU Chang-xi WANG Wen XIE Zhuo-jia CHEN Wen-ying SHU Min HUANG | 2013 | Acta Pharmacologica Sinica2013，34，4： | 15 |
| 15 | Health Effect of Forest Bathing Trip on Elderly Patients with Chronic Obstructive Pulmonary Disease显示文摘Forest bathing trip is a short,leisurely visit to forest.In this study we determined the health effects of forest bathing trip on elderly patients with chronic obstructive pulmonary disease(COPD).The patients were randomly divided into two groups.One group was sent to forest,and the other was sent to an urban area as control. | JIA Bing Bing YANG Zhou Xin MAO Gen Xiang LYU Yuan Dong WEN Xiao Lin XU Wei Hong LYU XIAO Ling CAO Yong Bao WANG Guo Fu | 2016 | Biomedical and Environmental Sciences2016，29，3： | 15 |
| 16 | Chemical constituents of Rhodiola kirilowii Maxim.显示文摘 | Lian-Mei Yang Rong Hu Wen Qi Peng Xing Hong-Zheng Fu | 2011 | Journal of Chinese Pharmaceutical Sciences2011，20，2： | 14 |
| 17 | Characteristics of Cd isotopic compositions and their genetic significance in the lead-zinc deposits of SW China显示文摘Up to now,the evaporation and condensation,as well as the biological absorption and inorganic absorptions,have been proved to be major factors in Cd isotope fractionation.And Cd isotopes have been widely applied in studies on the universal evolution and marine environment and so on.However,only a few researches have been conducted in applying Cd isotopes to trace the source of metallogenic material and the evolution of the ore-forming fluid in a complex mineralization environment,especially in a hydrothermal ore-formation system.We measured the Cd isotopic compositions of sphalerite,galena,and ores from five lead-zinc deposits in SW China,and found that the 114/110Cd values varied from 1.53‰to 0.34‰,with a total range of1.87‰,which is greater than most of measured geological samples.Meanwhile,through contrasting the Cd content with Cd isotopic compositions of different deposits,it may be concluded that different genetic lead-zinc deposits have different Cd content and isotopic compositions,which could be a tool for the studies on the origin of ore deposits.Also,the biomineralization and crystal fractionation may also result in Cd isotope fractionation.In a word,although the research of Cd isotopes is presently at the preliminary stage(especially in hydrothermal ore-formation system),this study demonstrated that Cd isotopes can give a clue in tracing the evolution of ore-forming fluid and metallogenic environment. | ZHU ChuanWei WEN HanJie ZHANG YuXu FAN HaiFeng FU ShaoHong XU Juan QIN TingRong | 2013 | Science China Earth Sciences2013，56，12： | 14 |
| 18 | Serum levels of mi RNA in patients with hepatitis B virus-associated acute-on-chronic liver failure显示文摘Background: Hepatitis B virus(HBV)-associated acute-on-chronic liver failure(HBV-ACLF) is a lifethreatening condition and its exact pathophysiology and progression remain unclear. The present study aimed to assess the role of serum mi RNAs in the evaluation of HBV-ACLF and to develop a model to predict the outcomes for ACLF.Methods: Serum was collected from 41 chronic hepatitis B and 55 HBV-ACLF patients in addition to30 chronic asymptomatic HBV carriers as controls. The mi RNAs expressions were measured by real-time quantitative PCR(q-PCR). Statistical analyses were conducted to assess the ability of differentially expressed mi RNAs and other prognostic factors in identifying ACLF prognosis and to develop a new predictive model.Results: Real-time q-PCR indicated that serum miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p levels were significantly upregulated in ACLF patients compared to chronic hepatitis B and chronic asymptomatic HBV carriers patients. In addition, multivariate regression analyses indicated that Na+, INR, gastrointestinal bleeding and mi R-122-3 p are all independent factors that are reliable and sensitive to the prognosis of HBV-ACLF. Therefore, we developed a new model for the prediction of HBV-ACLF disease state: Y = 0.402 × Na+-1.72 × INR-4.963 × gastrointestinal bleeding(Yes = 0; No = 1)-0.278 ×(mi R-122-3 p) + 50.449. The predictive accuracy of the model was 95.3% and the area under the receiver operating characteristic curve(AUROC) was 0.847.Conclusions: Expression levels of these mi RNAs(miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p) positively correlate with the severity of liver inflammation in patients with ACLF and may be useful to predict HBV-ACLF severity. | Ya Wen Shi-Fang Peng Lei Fu Xiao-Yu Fu Da-Xian Wu Bing-Jie Liu De-Ming Tan Yi Ouyang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，2： | 13 |
| 19 | Effect of human umbilical cord-derived mesenchymal stem cells on lung damage in severe COVID-19 patients:a randomized,double-blind,placebo-controlled phase 2 trial显示文摘Treatment of severe Coronavirus Disease 2019(COVID-19)is challenging.We performed a phase 2 trial to assess the efficacy andsafety of human umbilical cord-mesenchymal stem cells(UC-MScs)to treat severe coViD-19 patients with lung damage,based onour phase 1 data.In this randomized,double-blind,and placebo-controlled trial,we recruited 101 severe coVID-19 patients withlung damage.They were randomly assigned at a 2:1 ratio to receive either UC-MSCs(4×10^(7)cells per infusion)or placebo on day 0,3,and 6.The primary endpoint was an altered proportion of whole lung lesion volumes from baseline to day 28.Other imagingoutcomes,6-minute walk test(6-MWT),maximum vital capacity,diffusing capacity,and adverse events were recorded and analyzed.In all,100 COVID-19 patients were finally received either UC-MSCs in=65)or placebo(n=35).UC-MSCs administrationexerted numerical improvement in whole lung lesion volume from baseline to day 28 compared with the placebo(the mediandifference was-13.31%,95%Cl-29.14%,2.13%,P=0.08).UC-MSCs significanty reduced the proportions of solid componentlesion volume compared with the placebo(median difference:-15.45%;95%CI-30.82%,-0.39%;P=0.043).The 6-MWT showedan increased distance in patients treated with UC-MSCs(difference:27.00 m;95%CI 0.00,57.00;P=0.057).The incidence of adverseevents was similar in the two groups.These results suggest that UC-MSCs treatment is a safe and potentially effective therapeuticapproach for COVID-19 patients with lung damage.A phase 3 trial is required to evaluate effects on reducing mortality andpreventing long-term pulmonary disability. | Lei Shi Hai Huang Xuechun Lu Xiaoyan Yan Xiaojing Jiang Ruonan Xu Siyu Wang Chao Zhang Xin Yuan Zhe Xu Lei Huang Jun-Liang Fu Yuanyuan Li Yu Zhang Wei-Qi Yao Tianyi Liu Jinwen Song Liangliang Sun Fan Yang Xin Zhang Bo Zhang Ming Shi Fanping Meng Yanning Song Yongpei Yu Jiqiu Wen Qi Li Qing Mao Markus Maeurer Alimuddin Zumla Chen Yao Wei-Fen Xie Fu-Sheng Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 13 |
| 20 | Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation显示文摘AIM:To explore the protective effect of bone marrow mesenchymal stem cells(BM MSCs)in the small intestinal mucosal barrier following heterotopic intestinal transplantation(HIT)in a rat model.METHODS:BM MSCs were isolated from male Lewis rats by density gradient centrifugation,cultured,and analyzed by flow cytometry.The HIT models were divided into a non-rejection group,saline-treated rejection group(via penile vein),and BM MSC–treated group(via penile vein).Intestinal mucosal barrier injury was estimated by diamine oxidase(DAO)and D-lactic acid(D-LA)expression levels.Tumor necrosis factor-α(TNF-α),interferon-γ(INF-γ),interleukin-10(IL-10),and transforming growth factor-β(TGF-β)were detected by enzyme-linked immunosorbent assay.Ultrastructural change of tight junctions(TJs)was observed under transmission electron microscope.Expression levels of the TJ proteins occludin and zona occludens(ZO)-1,affected by the inflammatory factors,were measured using real-time polymerase chain reaction and Western blotting.RESULTS:The pathological score at each time point after surgery indicated significantly less serious injury in the BM MSCs-treated group than in the rejection group(P<0.05).In the former,graft levels of DAO and D-LA were reduced,and TNF-αand INF-γproduction was inhibited(at day 7:10.6473±0.0710vs 17.2128±0.4991,P<0.05;545.1506±31.9416vs 810.2637±25.1175,P<0.05).IL-10 and TGF-βproduction was increased greatly(at day 7:125.7773±4.7719 vs 80.3756±2.5866,P<0.05;234.5273±9.3980 vs 545.1506±31.9416,P<0.05).There was increased expression of occludin and ZO-1 protein(at day 7:0.2674±0.0128 vs 0.1352±0.0142,P<0.05;at day 5:0.7189±0.0289 vs 0.4556±0.0242,P<0.05)and mRNA(at day 7:0.3860±0.0254 vs 0.1673±0.0369,P<0.05;at day 5:0.5727±0.0419 vs0.3598±0.0242,P<0.05).CONCLUSION:BM MSCs can improve intestinal barrier permeability,repair TJs,and increase occludin and ZO-1 protein expression.With altered cytokine levels,they can protect the intestinal mucosa after transplantation. | Wen Zhang Zhong-Yang Shen Hong-Li Song Yang Yang Ben-Juan Wu Nan-Nan Fu Tao Liu | 2014 | World Journal of Gastroenterology2014，20，23： | 12 |
您所在的机构暂未开通此部分的功能，请联系管理员