|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 2 | The efficacy and safety of weekly 35-mg risedronate dosing regimen for Chinese postmenopausal women with osteoporosis or osteopenia: 1-year data显示文摘目的: 当每周,每天管理了时,口头的 risedronate 在绝经后的骨质疏松症的处理是有效的。在这 1 年,使随机化,双窗帘,我们比较了的 multicenter 学习在有骨质疏松症或 osteopenia 的中国绝经后的女人的处理的每周的 35-mg 和每日的 5-mg risedronate dosing 政体。 | Jie-mei GU Li WANG Hua LIN De-cai CHEN Hai TANG Xiao-lan JIN Wei-bo XIA Yun-qiu HU Wen-zhen FU Jin-wei HE Hao ZHANG Chun WANG Hua YUE Wei-wei HU Yu-juan LIU Zhen-lin ZHANG | 2015 | Acta Pharmacologica Sinica2015，36，7： | 19 |
| 3 | QTL editing confers opposing yield performance in different rice varieties显示文摘Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci(QTLs). In the past decade, many yield-contributing QTLs have been identified in crops.However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas_9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds. | Lan Shen Chun Wang Yaping Fu Junjie Wang Qing Liu Xiaoming Zhang Changjie Yan Qian Qian Kejian Wang | 2018 | Journal of Integrative Plant Biology2018，60，2： | 18 |
| 4 | Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion显示文摘 | Xin Fu Jun Xiao Yuning Wei Sheng Li Yan Liu Jie Yin Kun Sun Hao Sun Huating Wang Zongkang Zhang Bao-Ting Zhang Chun Sheng Hongyan Wang Ping Hu | 2015 | Cell Research2015，25，6： | 16 |
| 5 | Contribution of Myostatin gene polymorphisms to normal variation in lean mass, fat mass and peak BMD in Chinese male offspring显示文摘目的: Myostatin 基因是转变生长 factor-β 的一个成员;(TGF-β) 否定地调整骨胳的肌肉生长的家庭。在 Myostatin 的基因多型性被发现在中国女人与山峰骨头矿物质密度(BMD ) 被联系。这研究的目的是调查 Myostatin 是否在山峰 BMD,瘦团(LM ) ,和中国男人的胖质量(FM ) 在正常变化起了一个作用。 | Hua YUE Jin-wei HE Hao ZHANG Chun WANG Wei-wei HU Jie-mei GU Yao-hua KE Wen-zhen FU Yun-qiu HU Miao LI Yu-juan LIU Song-hua WU Zhen-lin ZHANG | 2012 | Acta Pharmacologica Sinica2012，33，5： | 10 |
| 6 | Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer显示文摘Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for efficiently inducing mutations in the genome.However,ZFN-mediated targeting in pigs has rarely been achieved.Here,we used ZFNs to knock out the porcineα-1,3-galactosyl-transferase(GGTA1)gene,which generates Gal epitopes that trigger hyperacute immune rejection in pig-to-human transplantation.Primary pig fibroblasts were transfected with ZFNs targeting the coding region of GGTA1.Eighteen mono-allelic and four biallelic knockout cell clones were obtained after drug selection with efficiencies of 23.4%and 5.2%,respectively.The biallelic cells were used to produce cloned pigs via somatic cell nuclear transfer(SCNT).Three GGTA1 null piglets were born,and one knockout primary fibroblast cell line was established from a cloned fetus.Gal epitopes on GGTA1 null pig cells were completely eliminated from the cell membrane.Functionally,GGTA1 knockout cells were protected from complement-mediated immune attacks when incubated with human serum.This study demonstrated that ZFN is an efficient tool in creating gene-modified pigs.GGTA1 null pigs and GGTA1 null fetal fibroblasts would benefit research and pig-to-human transplantation. | BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin | 2014 | Science China(Life Sciences)2014，57，2： | 9 |
| 7 | Novel mutations of TCIRG1 cause a malignant and mild phenotype of autosomal recessive osteopetrosis (ARO) in four Chinese families显示文摘 | Xiao-ya ZHANG Jin-wei HE Wen-zhen FU Chun WANG Zhen-lin ZHANG | 2017 | Acta Pharmacologica Sinica2017，38，11： | 8 |
| 8 | Design,synthesis and antiproliferative activities of diaryl urea derivatives bearing N-acylhydrazone moiety显示文摘A new series of diaryl urea derivatives bearing N-acylhydrazone moiety were designed and synthesized.All the target compounds were evaluated for their antiproliferative activities against human leukemia cell line(HL-60),human lung adenocarcinoma epithelial cell line(A549) and human breast cancer cell line(MDA-MB-231) in vitro by standard MTT assay.The pharmacological results indicated that some compounds exhibited promising antitumor activities.Compound 1j showed the most potent antiproliferative activity against the tested three cell lines with IC values of 0.13 mmol/L,0.7 mmol/L and 0.5 mmol/L,respectively. | Bei Zhang Yan Fang Zhao Xin Zhai Wei Jie Fan Jun Ling Ren Chun Fu Wu Ping Gong | 2012 | Chinese Chemical Letters2012，23，8： | 6 |
| 9 | Immobilization of laccase by Cu^(2+) chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol显示文摘把 polyacrylamide 用作金属螯的 ligand 的磁性的螯 Cu2+ 的硅石粒子被开发并且由协作为 laccase 的固定使用了。为五氯苯酚(PCP ) 的使不能调动的 laccase 和它的催化能力的酶的性质上的 pH 和温度的效果降级全身地被评估。与免费 laccase 相比,使不能调动的 laccase 显示出改进的酸适应性和热稳定性。在这个工作准备的使不能调动的 laccase 从水的答案为 PCP 移动展出了一个好催化能力。 | Ying Wang Di Zhang Fu Rong He Xiao Chun Chen | 2012 | Chinese Chemical Letters2012，23，2： | 5 |
| 10 | Polymorphism in the upstream regulatory region of human papilloma virus type 16 from the cervical cancer biopsies in Xinjiang Uygur women显示文摘To investigate the mutations in the upstream regulatory region (URR) of human papillomavirus type 16 (HPV-16) from the cervical cancer biopsies in Xinjiang Uygur women and its relationship to the high incidence of cervical cancer in the southern Xinjiang, the tissue DNA was extracted from the cervical cancer biopsies, and the URR segment of HPV-16 DNA was amplified, sequenced and analyzed. Thereafter, the polymorphism of URR in HPV-16 was then analyzed. It was demonstrated that the positive rate detected for the presence of URR in HPV-16 was 89.47% (17/19). Compared with the previously published sequence in URR of prototype HPV-16, some mutations were detected in the sequence of URR. The mutations in 17 URR fragments of HPV-16 could be divided into 11 patterns (XJU-1 to XJU-11) at nucleic acid level, in which each of XJU-1 and XJU-4 accounted for 23.53% (4/17), and other patterns of mutation accounted for 5.88% (1/17) . In comparison with the URR of prototype HPV-16, the DNA identity of these patterns was 98.50%-99.68% . In these 17 URR fragments, two point mutations occurred at position 7192 (G to T) and position 7520 (G to A) and they appeared to be constant in Xinjiang area. These two mutations were ubiquitous in the Asia-American type and conferred strong infection activity and carcinogenicity of this virus. In addition, the mutations at position 7729 (A to C), position 7843 (A to G) and position 7792 (C to T) could enhance its transcription activity considerably. It is concluded that some mutations occur in URR gene of HPV-16 in the cervical cancer biopsies taken from Uygur women in Xinjiang area, suggesting that certain relationship exists among the mutations in URR of HPV-16, the phylogeny of HPV-16 and the high incidence of cervical cancer in southern part of Xinjiang area. | MENG YU ZHENG HAI MA YAN PIN WANG XI DAN RE FU CHUN ZHANG | 2006 | Journal of Microbiology and Immunology2006，4，3： | 4 |
| 11 | Neuroprotective effect of Longshengzhi capsule following permanent middle cerebral artery occlusion in rats显示文摘Objective:Longshengzhi capsule(LSZC)is an optimized preparation based on the traditional Chinese Medicine formula Buyanghuanwu Decoction(BYHWD),and is approved by the China Food and Drug Administration for treating stroke-induced disability and vascular diseases.Herein,we examined the pharmacodynamics,anti-apoptotic and anti-oxidant actions,and potential mechanisms of action of LSZC following stroke in rats.Methods:Permanent middle cerebral artery occlusion(MCAO)was used as an ischemic stroke model.LSZC was administered intragastrically.We examined the survival rate,bodyweight,and neurological deficits in stroke rats.Brain infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining.Brain pathology was examined using hematoxylin and eosin staining,Nissl staining,and TdT-mediated dUTP Nick-End Labeling staining.Malondialdehyde,catalase,superoxide dismutase,and glutathione levels were examined by commercial kits.Expression of Nrf2,heme oxygenase-1,Bax,Bcl-2,cleaved caspase-3,and caspase-3 proteins in brain tissue was measured by Western blot.Results:LSZC markedly improved the survival rate and bodyweight,and reduced infarct volume and neurological deficit scores,in MCAO stroke rats.LSZC also significantly attenuated oxidative stress,as indicated by decreased expression of malondialdehyde,and upregulation of Nrf2,heme oxygenase-1,catalase,superoxide dismutase,and glutathione.Moreover,LSZC significantly decreased apoptosis,including a decrease in Bax and cleaved caspase-3 expression,and an increase in Bcl-2,as well as a reduction in numbers of apoptotic neurons.Conclusion:LSZC treatment is neuroprotective against ischemic stroke,potentially via reducing oxidative stress and apoptosis.The Nrf2 and apoptotic signaling pathways may play important roles in the antioxidant and anti-apoptotic actions of LSZC. | Xiaoli Sun Shuzhen Guo Yong Wang Bangze Fu Junjie Liu Yili Zhang Ruocong Yang Chun Li Jian Gao Li Gao Wei Wang | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 4 |
| 12 | Association of single nucleotide polymorphism Rs2236518 in PRDM16 gene with BMI in Chinese males显示文摘 | Hua YUE Jin-wei HE Yao-hua KE HaoZHANG Chun WANG Wei-weiHU Jie-mei GU Wen-zhen FU Yun-qiu HU Miao LI Yu-juan LIU Zhen-lin ZHANG | 2013 | Acta Pharmacologica Sinica2013，34，5： | 3 |
| 13 | Genetic variants in the PLS3 gene are associated with osteoporotic fractures in postmenopausal Chinese women显示文摘Plastin 3 (PLS3) has been identified as a candidate gene for bone fragility in the Rotterdam study (RS) population. So far, however, whether PLS3 polymorphisms are genetic risk factors for osteoporosis in Asian population remains unclear. In order to investigate the association between genetic variants in PLS3 and the risk of fragility fracture and/or bone mineral density (BMD) in postmenopausal Chinese women, we conducted a case-control association study. A total of 1083 postmenopausal patients with osteoporotic fractures and 2578 unrelated non-fracture controls in Shanghai were enrolled. Seven SNPs, including six tagSNPs in PLS3 and one identified genetic risk factor (rs140121121) for osteoporosis in the RS population, were genotyped in all the participants. BMD at lumbar spine and hip sites were measured in 2578 controls. Association between SNPs and the risk of osteoporotic fractures and/or BMD were analyzed. The GC genotype of rs757124 and AC genotype of rs10521693 were associated with lumbar vertebral fracture (P=0.020 and 0.046, respectively). The association between tagSNPs and BMD were analyzed only in 2546 controls to avoid biased conclusion. rs757124 was significantly associated with BMD at lumbar spine and hip sites. GG genotype had the highest BMD at lumbar spine (L1-4), while CC genotype had the highest BMD at hip sites. Our results suggest that polymorphisms in PLS3 are genetic loci for osteoporosis in postmenopausal Chinese women. | Chong Shao Yi-wen Wang Jin-wei He Wen-zhen Fu Chun Wang Zhen-lin Zhang | 2019 | Acta Pharmacologica Sinica2019，40，9： | 3 |
| 14 | Application of Grey Associative Analysis to the Assessment of Regional Water Security显示文摘The assessment of water security is an important content in the security management of water resources due to the fact that the state of water security directly affects both the sustainable development of regional economy and the improvement on the living quality of mankind. Grey associative analysis is introduced and applied to assessment of water security on the basis of grey characteristics of the assessment index system of water security. As a case study shows, grey associative analysis is used for evaluating water security of some provinces in China, and the satisfactory assessment results are obtained. The sequence of provinces in China with regard to water security from good to poor is obtained and, moreover, the water security level of each region is also confirmed. The results obtained accord with the actual state of each region. They are of practical significance and can be used to guide the management of regional water security and a sustainable development of the economy therein. At the same time, the results demonstrate that grey associative analysis provides a new method for assessing water | Zhang Yanmin~1, Dong Qianjin~1, Wang Xianjia~1, Wang Jianping~2, Fu Chun~3(1.State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China 2.College of Economics and Management, China Three Gorges University, Yichang 443002, China 3. Center of China Central Economy Development, Nanchang University, Nanchang 330047, China) | 2007 | 工程科学(英文版)2007，5，2： | 2 |
| 15 | No association between polymorphisms and haplotypes of COLIA1 and COLIA2 genes and osteoporotic fracture in postmenopausal Chinese women显示文摘瞄准: 学习 COL1A1 和 COL1A2 基因的基因多型性是否在绝经后的中国女人影响了骨折的发作。 | Wei-wei HU Jin-wei HE HaoZHANG Chun WANG Jie-mei GU Hua YUE Yao-hua KE Yun-qiu HU Wen-zhen FU Miao LI Yu-juan LIU Zhen-lin ZHANG | 2011 | Acta Pharmacologica Sinica2011，32，7： | 2 |
| 16 | Surface sulfur vacancies enhanced electron transfer over Co-ZnS quantum dots for efficient degradation of plasticizer micropollutants by peroxymonosulfate activation显示文摘Peroxymonosulfate(PMS)activation in heterogeneous processes is a promising water treatment technology.Nevertheless,the high energy consumption and low efficiency during the reaction are ineluctable,due to electron cycling rate limitation.Herein,a new strategy is proposed based on a quantum dots(QDs)/PMS system.Co-ZnS QDs are synthesized by a water phase coprecipitation method.The inequivalent lattice-doping of Co for Zn leads to the generation of surface sulfur vacancies(SVs),which modulates the surface of the catalyst to form an electronic nonequilibrium surface.Astonishingly,the plasticizer micropollutants can be completely degraded within only tens of seconds in the Co-Zn S QDs/PMS system due to this type of surface modulation.The interfacial reaction mechanism is revealed that pollutants tend to be adsorbed on the cobalt metal sites as the electron donors,where the internal electrons of pollutants are captured by the metal species and transferred to the surface SVs.Meanwhile,PMS adsorbed on the SVs is reduced to radicals by capturing electrons,achieving effective electron recovery.Dissolved oxygen(DO)molecules are also easily attracted to catalyst defects and are reduced to O_(2)^(·-),further promoting the degradation of pollutants. | Yuting Gu Tingting Gao Fagen Zhang Chao Lu Wenrui Cao Ziwei Fu Chun Hu Lai Lyu | 2022 | Chinese Chemical Letters2022，33，8： | 2 |
| 17 | Bright Red Organic Electroluminescent Devices Based on a Soluble Rare Earth Complex Eu(TTA)_3 Phen显示文摘IntroductionOrganicelectroluminescent(EL)deviceshavebecomeoneofthemostimportantsub-jectsbecauseoftheirpotentialapplicationasl... | LI Bin, MA Dong ge, ZHANG Hong jie ** , ZHAO Xiao jiang and NI Jia zuan (Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022) FU Chun yan (Songliao River | 1998 | Chemical Research in Chinese Universities1998，14，3： | 2 |
| 18 | Further evidence for paternal DNA transmission in gynogenetic grass carp显示文摘Gynogenesis is an important breeding method in aquaculture and has been widely applied to many fish species.If gynogenetic progenies are to inherit paternal partial genomic DNA,this will increase genetic variation and will provide a useful outcome for breeding.In this study,we investigated the genetic variation in homeobox(Hox)gene clusters(Hox A4 a,Hox A9 a,Hox A11 b,Hox B1 b,Hox C4 a,Hox C6 b,and Hox D10 a)among koi carp(Cyprinus carpio haematopterus,KOC;the stimulation sperm source),grass carp(Ctenopharyngodon idellus),and gynogenetic grass carp(GGC).We found paternal DNA(a special DNA fragment and Hox C6 b)derived from KOC and a recombinant gene belonging to Hox C6 b in GGC.We are the first to report the recombinant Hox C6 b in GGC.Our study provides further evidence for paternal DNA transmission to gynogenetic progenies,which is a finding with great significance for the genetic breeding of fish. | Zhuangwen Mao Yeqing Fu Shi Wang Yude Wang Kaikun Luo Chun Zhang Min Tao Shaojun Liu | 2020 | Science China(Life Sciences)2020，63，9： | 2 |
| 19 | Detection of Human Telomerase Reverse Transcriptase (hTERT) mRNA With ^99mTc-Radiolabeled Antisense Probes in Malignant Tumors in vivo显示文摘 | Wang Rong Fu Liu Meng Zhang Chun Li | 2008 | J Nucl Med2008，49，1： | 1 |
| 20 | Association of ALOX15 gene polymorphisms with obesity-related phenotypes in Chinese nuclear families with male offspring显示文摘目的: 在 ALOX12 的基因变化,编码了人的 12-lipoxygenase,在年轻中国人被发现与脂肪被联系集体。这研究的目的是与男后代在中国原子家庭在 ALOX15 基因和肥胖相关的显型调查在单个核苷酸多型性(SNP ) 和 haplotypes 之间的关系。 | Yao-hua KE Wen-iin XIAO Jin-wei HE Hao ZHANG Jin-bo YU Wei-wei HU Jie-mei GU Gao GAO Hua YUE Chun WANG Yun-qiu HU Miao LI Yu-juan LIU Wen-zhen FU Zhen-lin ZHANG | 2012 | Acta Pharmacologica Sinica2012，33，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员