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| 1 | Research Progress on the Relationship between Coronary Artery Calcification and Chronic Renal Failure显示文摘 | Jun Lai Gael Akindavyi Qiang Fu Zhi-Liang Li Hui-Min Wang Li-Hua Wen | 2018 | Chinese Medical Journal2018，，5： | 18 |
| 2 | 真武汤可延缓尿毒症心肌病大鼠心室肥厚显示文摘目的探讨真武汤对尿毒症心肌病大鼠心室肥厚的作用及可能机制。方法(1)提取SD乳鼠心肌细胞,将其分为对照组及硫酸吲哚酚(indoxyl sulfate, IS)组(10μm刺激),通过[3H]亮氨酸掺入法、Western blot(WB)检测两组细胞蛋白表达情况;(2)将50只SD大鼠随机分成假手术组、模型组、真武汤低(4.5 g/kg)、高剂量干预组(13.5 g/kg),除假手术组外,其他各组均予5/6肾切除术。术后4周,低剂量组及高剂量组分别每天给与真武汤灌胃,假手术组和模型组给予等量蒸馏水灌胃。给药4周后,检测各组大鼠血生化、血浆硫酸吲哚酚水平(IS),心脏及心室重量指数、心脏彩超、心肌组织BNP、p-ERK1/2、p-p38及p-JNK蛋白表达等情况。结果(1)细胞实验中,IS组心肌细胞蛋白质合成速率明显增加,而BNP、p-ERK1/2、p-p38表达均较对照组升高(均P<0.01),而P-JNK蛋白则与对照组相比未见明显差异;(2)与假手术组相比,模型组的血肌酐(SCr)、尿素氮(BUN)、24小时尿蛋白(24hUpro)、IS及左室质量指数(LVMI)、全心质量指数(HMI)均升高(P<0.01);左心室收缩末期内径(LVESD)、左心室舒张末期内径(LVEDD)均有显著降低,左心室前壁收缩末期厚度(LVAWS)、左心室前壁舒张末期厚度(LVAWD)、左心室后壁收缩期厚度(LVPWS)、左心室后壁舒张期厚度(LVPWD)则明显增厚;HE染色示心肌细胞肥大。心肌组织蛋白BNP、p-ERK1/2、p-p38、p-jnk的表达均升高(P<0.01);(3)低剂量和高剂量组SCr、BUN、24 hUpro及IS较模型组降低(P<0.05),LVMI及HMI在低剂量、高剂量组中下降;LVESD、LVEDD、LVPWS、LVAWS、LVAWD等在高剂量组中改善较为显著,心肌组织蛋白BNP、p-ERK1/2、pp38及p-JNK的表达下调。结论真武汤可降低尿毒症心肌病大鼠血浆IS水平,进而延缓IS对心肌肥厚作用,延缓心室重构。 | 赖俊 吴英智 杭丽玮 Gael Akindavyi 邓婷 严全能 傅强 李志樑 | 2019 | 南方医科大学学报2019，39，1： | 8 |
| 3 | 炎症小体Nlrp3通路激活在尿毒症心肌病模型小鼠左心室重构的作用显示文摘目的探讨Nlrp3炎症小体通路激活与尿毒症心肌病小鼠左心室重构的关系及可能机制。方法将20只小鼠随机分为假手术组和模型组。模型组予5/6肾脏切除术。两组小鼠术后第8周行心脏超声检查,检测血肌酐(Scr)、血尿素氮(BUN)、血浆硫酸吲哚酚(indoxyl sulfate,IS)水平,心肌组织ANP、BNP、Nlrp3、Pro-caspase1、IL-1β和p-P65蛋白表达。培养小鼠巨噬细胞RAW264.7,分为对照组及IS组,检测两组细胞Nlrp3、Pro-caspase1和IL-1β蛋白表达。结果与假手术组相比,模型组小鼠Scr、BUN和IS水平均升高(P<0.05);左心室前壁舒张末期厚度(LVAWd)和左心室后壁舒张末期厚度(LVPWd)增厚,射血分数(EF)和短轴缩短率(FS)降低。更进一步,左心室等容舒张时间和E/A上升,LVFS/MPI下降。HE染色可见心肌细胞肥大。模型组心肌组织ANP、BNP、Nlrp3、Pro-caspase1、IL-1β和p-P65蛋白表达均上调。IS刺激小鼠巨噬细胞Nlrp3、Pro-caspase1和IL-1β蛋白表达均上调。以上结果均具有统计学意义。结论尿毒症心肌病小鼠左心室肥厚与血浆IS水平上调密切相关,可能与NF-κB信号通路激活炎症小体Nlrp3高度相关。 | 文俪桦 傅强 严全能 李晚泉 贺不凡 Gael Akindavyi 李志樑 | 2020 | 实用医学杂志2020，36，9： | 4 |
| 4 | 4例顽固性室性心动过速患者行ECMO+心外膜射频消融的术中护理显示文摘目的总结4例顽固性室性心动过速患者在静脉-动脉体外肺膜氧合(VA-ECMO)辅助下行心包穿刺心外膜射频消融术的术中护理经验。方法4例患者在心血管内科、ICU、麻醉科、介入手术室、护理等多学科团队协作下,术前制定周密的手术方案、应急预案和具体分工,由多学科团队联合提供手术全程监护,术中护理重点包括:注重术中的低体温、出血、溶血及灌注量变化、管道的管理、并发症的预防等问题,严密观察病情和配合手术,加强生命体征监测和出凝血监测,进行管道和穿刺口安全管理。结果4例患者手术成功,术中未发生严重并发症,住院时间为(12.50±2.06)d。1例在术后当天出现心源性休克,及时以CRRT配合治疗后好转出院。结论VA-ECMO能在术中提供血流动力学支持,提高手术成功率。术中护士与团队紧密配合,加强术中体温、出血/溶血、灌注量的监护,实施并发症的预见护理,可为手术提供安全保障。 | 王玉珊 陈健聪 付晓华 冷坷 何维娟 Akindavyi Gael | 2020 | 护理学报2020，27，7： | 4 |
| 5 | 高通量技术筛选主动脉夹层生物标志物的研究显示文摘目的:通过iTRAQ联合Label-free法筛选主动脉夹层潜在诊断标志物。方法:入选急性主动脉夹层及对照组(急性冠状动脉综合征)血清各50例。将两组患者中各15例血清标本用于iTRAQ及Label-free分析筛选表达差异蛋白。对所有收集血清进行差异蛋白的免疫吸附测定(ELISA)验证。结果:两组血清标本使用iTRAQ方法共鉴定出84种差异表达蛋白质,Label-free方法共鉴定有或无差异蛋白20种。通过Panther软件进行生信分析与主动脉夹层发病机制9种相关蛋白作为候选生物标志物。进行ELISA验证后得出2种差异蛋白Lumican、PI16有统计学差异,且特异性和敏感性较高。结论:蛋白Lumican、PI16可能成为主动脉夹层潜在的诊断标志物。 | 傅强 邓小芳 Akindavyi Gael 刘云峰 王慧敏 邓钰莹 李志樑 邓婷 | 2021 | 临床心血管病杂志2021，37，10： | 1 |
| 6 | Gingerol improves cardiac function in rats with cardiorenal syndrome via inhibiting fibrosis显示文摘Background Cardiorenal syndrome(CRS)is a clinical syndrome with a complex mechanism,and there is currently no specific treatment.Gingerol was confirmed to possess anti-inflammatory,antioxidant and cardiotonic properties as cardiovascular pharmacological effects.However,in vivo studies have yet to prove that it can improve cardiac function and inhibit fibrosis in rats with cardiorenal syndrome.Methods In this study,34 male Sprague-Dawley(SD)rats were randomly divided into control(n=9),model(n=12)and gingerol groups(n=13).The model and the gingerol groups underwent ligation of the left anterior descending coronary artery and 5/6 subtotal nephrectomy to construct a type 2 cardiorenal syndrome rat model.The rats in gingerol group were injected intraperitoneally with 50 mg/kg gingerol.The same amount of saline was administered to both the control and the model groups.Following 4 weeks of treatment,the rat cardiac function and myocardial fibrosis were evaluated by cardiac ultrasound and blood biochemistry.Results Biochemical results showed that the brain natriuretic peptide(BNP)levels of gingerol group decreased(P<0.05).Cardiac ultrasound revealed that gingerol improved cardiac systolic function and ventricular remodeling(P<0.05).The systolic function of the model group was significantly decreased compared with the control group.Masson staining confirmed that the fibrosis area in the model group was significantly augmented than that in the control group,while the area of fibrosis in the gingerol group was diminished compared to the model group(P<0.01).Moreover,immunofluorescence showed that compared with the control group,the expression of collagen 1,TGF-β1 andα-SMA was significantly increased in the model group,and both collagen deposition and the expression of collagen I,TGF-β1 andα-SMA decreased in gingerol group.Immunohistochemistry revealed that the expression of collagen 1 andα-SMA was significantly increased in the model group compared with the control group,while it was decreased in gingerol group(P<0.05).Conclusions Gingerol can improve the cardiac function and cardiac fibrosis in rats with cardiorenal syndrome. | 邓婷 吴英智 Akindavyi Gael 刘云峰 李志樑 傅强 邓小芳 | 2021 | South China Journal of Cardiology2021，22，2： | 0 |
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