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| 1 | Converting lateral scanning into axial focusing to speed up three-dimensional microscopy显示文摘In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging.Recently,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been achieved.However,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging.In an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging.Using a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image space.We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three dimensions.We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution. | Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.Kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka | 2020 | Light(Science & Applications)2020,9,1: | 4 |
| 2 | Periostin Is Induced in Glomerular Injury and Expressed de Novo in Interstitial Renal Fibrosis显示文摘 | Kontheari Sen Maja T. Lindenmeyer Ariana Gaspert Felix Eichinger Matthias A. Neusser Matthias Kretzler Stephan Segerer Clemens D. Cohen | 2011 | The American Journal of Pathology2011,,4: | 2 |
| 3 | Fibrinogen plasma levels are an independent prognostic parameter in patients with cervical cancer显示文摘 | Stephan Polterauer Veronika Seebacher Katrin Hefler-Frischmuth Christoph Grimm Georg Heinze Clemens Tempfer Alexander Reinthaller Lukas Hefler | 2009 | American Journal of Obstetrics and Gynecology2009,,6: | 2 |
| 4 | Molecular mechanisms of plant metal tolerance and homeostasis显示文摘 | Stephan Clemens | 2001 | Planta2001,,4: | 2 |
| 5 | Plant science: the key to preventing slow cadmium poisoning显示文摘 | Stephan Clemens Mark G.M. Aarts Sébastien Thomine Nathalie Verbruggen | 2012 | Trends in Plant Science2012,,: | 2 |
| 6 | Reflective and transmissive CR scanhead technology on needle image plates显示文摘 | Jorg F Stephan M Clemens H | 2005 | SPIE2005,5745,: | 1 |
| 7 | Arabidopsis thaliana expresses a second functional phytochelatin synthase显示文摘 | Anne-Claire Cazalé Stephan Clemens | 2001 | FEBS Letters2001,,2: | 1 |
| 8 | Chemical Trees Minimizing Energy and Hosoya Index 显示文摘 | CLEMENS H STEPHAN G W | 2009 | J Math Chem2009,46,: | 1 |
| 9 | A long way ahead: understanding and engineering plant metal accumulation显示文摘 | Stephan Clemens Michael G Palmgren Ute Kr?mer | 2002 | Trends in Plant Science2002,,7: | 1 |
| 10 | A Cyanobacterial Protein with Similarity to Phytochelatin Synthases Catalyzes the Conversion of Glutathione to C-Glutamylcysteine and Lacks Phytochelatin Synthase Activity显示文摘 | EMIKO HARADA EDDA VON ROEPENACK-LAHAYE STEPHAN CLEMENS | 2004 | Phytochemistry2004,65,: | 1 |
| 11 | Localization and functional characterization of metal-binding sites in phytochelatin synthases显示文摘 | Thomas Maier Chao Yu Gerhard Küllertz Stephan Clemens | 2003 | Planta2003,,2: | 1 |
| 12 | Proteome changes in Arabidopsis thaliana roots upon exposure to Cd^2+显示文摘 | Udo Roth Edda yon Roepenack - Lahaye Stephan Clemens | 2006 | Journal of Experimental Botany2006,57,15: | 1 |
| 13 | Molecular mechanisms of plant metal tolerance and homeostasis 显示文摘 | Stephan Clemens | 2001 | Planta2001,4,: | 1 |
| 14 | Evolution and function of phytochelatin synthases显示文摘 | Stephan Clemens | 2005 | Journal of Plant Physiology2005,,3: | 1 |
| 15 | A novel training model for the loop electrosurgical excision procedure显示文摘 | Lukas Hefler Christoph Grimm Verena Kueronya Clemens Tempfer Alexander Reinthaller Stephan Polterauer | 2012 | American Journal of Obstetrics and Gynecology2012,,6: | 1 |
| 16 | Wendtner,Hartmut Dohner,Gunter Brittinger,Bertold Emmerich,Michael Hallek and the German CLL study group:Fludarabine plus cyclophosphamide versus fludarabine abone in first-line therapy of younger patients with chronic lymphocytic leukemia显示文摘 | Barbara F Eichhorst Raymonde Busch Georg Hopfinger Rita Pasold Manfred Hensel Cordelia Steinbrecher Siegfried Siehl Ulrich Jager Mannela Bergmann Stephan Stilgenbauer Carmen Schweighofer Clemens M | 2006 | Blood2006,107,: | 1 |
| 17 | Loci From a Genome-Wide Analysis of Bilirubin Levels Are Associated With Gallstone Risk and Composition显示文摘 | Stephan Buch Clemens Schafmayer Henry V?lzke Marcus Seeger Juan F. Miquel Silvia C. Sookoian Jan H. Egberts Alexander Arlt Carlos J. Pirola Markus M. Lerch Ulrich John Andre Franke Oliver von Kampen Mario Brosch Michael Nothnagel Wolfgang Kratzer Bernhard | 2010 | Gastroenterology2010,,6: | 1 |
| 18 | River restoration challenges with a specific view on hydromorphology显示文摘1 Introduction 1.1 River ecosystem degradation Over the past century,almost throughout the whole world,streams and rivers have been heavily polluted and morphologically degraded due to industrial,household and agricultural sources,leading to significant declines in biodiversity,water quality and ultimately water supply[1-5].In the last decades,river system design has been practiced from an engineering viewpoint that focuses on water abstraction,sanitation,generation of energy,transportation and safety,rather than from a socio-ecological perspective.The technical management altered the understanding of a functioning river system itself and its social context. | Jianhua LI Stephan HOERBINGER Clemens WEISSTEINER Lingmin PENG Hans Peter RAUCH | 2020 | Frontiers of Structural and Civil Engineering2020,14,5: | 0 |
| 19 | (Not) an elementary question:How to mobilize the iron in the wall?显示文摘Photosynthesis,respiration,and countless other processes are dependent on proteins that carry iron(Fe)as an essential cofactor.When Fe was recruited for biological functions early in evolution,it was an available element.The Earth's atmosphere was reducing with oxygen limited to very low pressure.Thus,Fe was present as comparatively soluble Fe sulfides. | Stephan Clemens | 2021 | Molecular Plant2021,14,10: | 0 |
| 20 | Immunological memory: resting on two shoulders at antigen entrance sites?显示文摘Memory is a term used in two important systems:the nervous system and the immune system.Both systems need to recognize alterations and threats in an ever-changing environment.As a consequence,both systems during their evolution perfected their abilities to recall earlier challenges in mounting protective responses.This ability gives both systems the power to facilitate these protective responses if confronted with the same or similar challenges again. | Clemens Wulfing Stephan Henne Jasmin Oehlmann Hauke Simon Gunther | 2019 | Cellular & Molecular Immunology2019,16,10: | 0 |