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| 1 | Increased liver stiffness in alcoholic liver disease:Differentiating fibrosis from steatohepatitis显示文摘AIM:To test if inflammation also interferes with liver stiffness (LS) assessment in alcoholic liver disease (ALD) and to provide a clinical algorithm for reliable fibrosis assessment in ALD by FibroScan (FS).METHODS:We first performed sequential LS analysis before and after normalization of serum transaminases in a learning cohort of 50 patients with ALD admitted for alcohol detoxification. LS decreased in almost all patients within a mean observation interval of 5.3 d. Six patients (12%) would have been misdiagnosed with F3and F4 fibrosis but LS decreased below critical cut-off values of 8 and 12.5 kPa after normalization of trans-aminases. RESULTS:Of the serum transaminases,the decrease in LS correlated best with the decrease in glutamic oxaloacetic transaminase (GOT). No significant chang-es in LS were observed below GOT levels of 100 U/L. After establishing the association between LS and GOT levels,we applied the rule of GOT < 100 U/L for reliable LS assessment in a second validation cohort of 101 patients with histologically confi rmed ALD. By ex-cluding those patients with GOT > 100 U/L at the time of LS assessment from this cohort,the area under the receiver operating characteristic (AUROC) for cirrhosis detection by FS improved from 0.921 to 0.945 while specificity increased from 80% to 90% at a sensitivity of 96%. A similar AUROC could be obtained for lower F3 fibrosis stage if LS measurements were restricted to patients with GOT < 50 U/L. Histological grading of inflammation did not further improve the diagnostic accuracy of LS.CONCLUSION:Coexisting steatohepatitis markedly increases LS in patients with ALD independent of fibrosis stage. Postponing cirrhosis assessment by FS during alcohol withdrawal until GOT decreases to < 100 U/mL signif icantly improves the diagnostic accuracy. | Sebastian Mueller Gunda Millonig Lucie Sarovska Stefanie Friedrich Frank M Reimann Maria Pritsch Silke Eisele Felix Stickel Thomas Longerich Peter Schirmacher Helmut Karl Seitz | 2010 | World Journal of Gastroenterology2010，16，8： | 10 |
| 2 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 3 | DC-derived IL-18 drives Treg differentiation, murine Helicobacter pylori-specific immune tolerance, and asthma protection显示文摘 | Oertli Mathias Sundquist Malin Hitzler Iris Engler Daniela B Arnold Isabelle C Reuter Sebastian Maxeiner Joachim Hansson Malin Taube Christian Quiding-J?rbrink Marianne Müller Anne | 2012 | EN2012，，3： | 5 |
| 4 | 针对患者利益的机器学习和人工智能研究:在透明性、可重复性、伦理和有效性等方面的20个关键问题显示文摘机器学习(ML)、人工智能(AI)和其他现代统计方法正为利用先前尚未开发且极速增长的数据资源提供新的机会,以期让患者获益。尽管目前正在进行许多有前景的研究,特别是在图像方面,但就文献整体而言还缺乏透明度、对可重复性清晰的阐述、对潜在伦理问题的探究,以及对有效性的明确验证。这些问题的存在有许多原因,其中最重要的一点(为此我们提供了初步解决方案)就是当前缺乏针对ML和AI的最佳实践指南。我们认为从事研究的跨学科团队和应用ML/AI影响健康的项目,将因解决有关透明度、可重复性、伦理和有效性(TREE)的一系列问题而受益。这里提出的20个关键问题为研究团队提供了一个研究设计、实施和报告框架;帮助编辑和同行评审专家评估文献的贡献;让患者、临床医生和政策制定者评估新发现可能会给患者带来的获益。 | Sebastian Vollmer Bilal A Mateen Gergo Bohner Franz J Kirdly Rayid Ghani Pall Jonsson Sarah Cumbers Adrian Jonas Katherine S L McAllister Puja Myles David Granger Mark Birse Richard Branson Karel G M Moons Gary S Collins John P A Chris Holmes Harry Hemingwayp 李峰(译) 徐磊(校) 赵邑(校) | 2020 | 英国医学杂志中文版2020，23，9： | 5 |
| 5 | Helicobacter pylori infection prevents allergic asthma in mouse models through the induction of regulatory T cells显示文摘 | Arnold Isabelle C Dehzad Nina Reuter Sebastian Martin Helen Becher Burkhard Taube Christian Müller Anne | 2011 | Journal of Clinical Investigation2011，，8： | 4 |
| 6 | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber显示文摘Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4.Advances in wavefrontshaping methods and computational power have recently allowed for a novel approach to high-resolution imaging,utilizing deterministic light propagation through optically complex media and,of particular importance for this work,multimode optical fibers(MMFs)5–7.We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications.The volume of tissue lesion was reduced by more than 100-fold,while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF.Here,we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures,dendrites and synaptic specializations,in deep-brain regions of living mice,as well as monitored stimulus-driven functional Ca2+responses.These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage,heralding new possibilities for deep-brain imaging in vivo. | Sebastian A.Vasquez-Lopez Raphaël Turcotte Vadim Koren Martin Plöschner Zahid Padamsey Martin J.Booth TomášČižmár Nigel J.Emptage | 2018 | Light(Science & Applications)2018，7，1： | 4 |
| 7 | HBsAg quantification for identification of liver disease in chronic hepatitis B virus carriers显示文摘 | Simon B. Larsson Anders Eilard Sebastian Malmstr?m Charles Hannoun Amar P. Dhillon Gunnar Norkrans Magnus Lindh | 2014 | Liver Int2014，，7： | 3 |
| 8 | Digital templating in total hip arthroplasty:Additional anteroposterior hip view increases the accuracy显示文摘AIM To analyze planning total hip arthroplasty(THA) with an additional anteroposterior hip view may increases the accuracy of preoperative planning in THA.METHODS We conducted prospective digital planning in 100 consecutive patients: 50 of these procedures were planned using pelvic overview only(first group), and the other 50 procedures were planned using pelvic overview plus antero-posterior(a.p.) hip view(second group). The planning and the procedure of each patient were performed exclusively by the senior surgeon. Fifty procedures with retrospective analogues planning were used as the control group(group zero). After the procedure, the planning was compared with the eventually implanted components(cup and stem). For statistic analysis the x^2 test was used for nominal variables and the t test was used for a comparison of continuous variables.RESULTS Preoperative planning with an additional a.p. hip view(second group) significantly increased the exact component correlation when compared to pelvic overview only(first group) for both the acetabular cup and the femoral stem(76% cup and 66% stem vs 54% cup and 32% stem). When considering planning ± 1 size, the accuracy in the second group was 96%(48 of 50 patients) for the cup and 94% for the stem(47 of 50patients). In the analogue control group(group zero), an exact correlation was observed in only 1/3 of the cases.CONCLUSION Digital THA planning performed by the operating surgeon and based on additional a.p. hip view significantly increases the correlation between preoperative planning and eventual implant sizes. | Sophia K Stigler Franz J Müller Sebastian Pfaud Michael Zellner Bernd Füchtmeier | 2017 | World Journal of Orthopedics2017，8，1： | 3 |
| 9 | Expression of Tissue Factor in High-Grade Carotid Artery Stenosis: Association With Plaque Destabilization显示文摘 | Sebastian Jander Matthias Sitzer Angélique Wendt Michael Schroeter Martin Buchkremer Mario Siebler Wolfram Müller Wilhelm Sandmann Guido Stoll | 2001 | Stroke: Journal of the American Heart Association2001，，4： | 3 |
| 10 | A framework infrageneric classification of Carex (Cyperaceae) and its organizing principles显示文摘Phylogenetic studies of Carex L.(Cyperaceae)have consistently demonstrated that most subgenera and sections are para-or polyphyletic.Yet,taxonomists continue to use subgenera and sections in Carex classification.Why?The Global Carex Group(GCG)here takes the position that the historical and continued use of subgenera and sections serves to(i)organize our understanding of lineages in Carex,(ii)create an identification mechanism to break the~2000 species of Carex into manageable groups and stimulate its study,and(iii)provide a framework to recognize morphologically diagnosable lineages within Carex.Unfortunately,the current understanding of phylogenetic relationships in Carex is not yet sufficient for a global reclassification of the genus within a Linnean infrageneric(sectional)framework.Rather than leaving Carex classification in its current state,which is misleading and confusing,we here take the intermediate steps of implementing the recently revised subgeneric classification and using a combination of informally named clades and formally named sections to reflect the current state of our knowledge.This hybrid classification framework is presented in an order corresponding to a linear arrangement of the clades on a ladderized phylogeny,largely based on the recent phylogenies published by the GCG.It organizes Carex into six subgenera,which are,in turn,subdivided into 62 formally named Linnean sections plus 49 informal groups.This framework will serve as a roadmap for research on Carex phylogeny,enabling further development of a complete reclassification by presenting relevant morphological and geographical information on clades where possible and standardizing the use of formal sectional names. | Eric H.Roalson Pedro Jiménez-Mejías Andrew L.Hipp Carmen Benítez-Benítez Leo P.Bruederle Kyong-Sook Chung Marcial Escudero Bruce A.Ford Kerry Ford Sebastian Gebauer Berit Gehrke Marlene Hahn Muhammad Qasim Hayat Mathias H.Hoffmann Xiao-Feng Jin Sangtae Kim Isabel Larridon Étienne Léveillé-Bourret Yi-Fei Lu Modesto Luceño Enrique Maguilla Jose IgnacioMárquez-Corro Santiago Martín-Bravo Tomomi Masaki Mónica Míguez Robert F.C.Naczi Anton A.Reznicek Daniel Spalink Julian R.Starr Uzma Tamara Villaverde Marcia J.Waterway Karen L.Wilson and Shu-Ren Zhang | 2021 | Journal of Systematics and Evolution2021，59，4： | 3 |
| 11 | National Forest Inventories capture the multifunctionality of managed forests in Germany显示文摘Background:Forests perform various important ecosystem functions that contribute to ecosystem services.In many parts of the world,forest management has shifted from a focus on timber production to multi-purpose forestry,combining timber production with the supply of other forest ecosystem services.However,it is unclear which forest types provide which ecosystem services and to what extent forests primarily managed for timber already supply multiple ecosystem services.Based on a comprehensive dataset collected across 150 forest plots in three regions differing in management intensity and species composition,we develop models to predict the potential supply of 13 ecosystem services.We use those models to assess the level of multifunctionality of managed forests at the national level using national forest inventory data.Results:Looking at the potential supply of ecosystem services,we found trade-offs(e.g.between both bark beetle control or dung decomposition and both productivity or soil carbon stocks)as well as synergies(e.g.for temperature regulation,carbon storage and culturally interesting plants)across the 53 most dominant forest types in Germany.No single forest type provided all ecosystem services equally.Some ecosystem services showed comparable levels across forest types(e.g.decomposition or richness of saprotrophs),while others varied strongly,depending on forest structural attributes(e.g.phosphorous availability or cover of edible plants)or tree species composition(e.g.potential nitrification activity).Variability in potential supply of ecosystem services was only to a lesser extent driven by environmental conditions.However,the geographic variation in ecosystem function supply across Germany was closely linked with the distribution of main tree species.Conclusions:Our results show that forest multifunctionality is limited to subsets of ecosystem services.The importance of tree species composition highlights that a lack of multifunctionality at the stand level can be compensated by managing forests at the landscape level,when stands of complementary forest types are combined.These results imply that multi-purpose forestry should be based on a variety of forest types requiring coordinated planning across larger spatial scales. | Nadja K.Simons María R.Felipe-Lucia Peter Schall Christian Ammer Jürgen Bauhus Nico Blüthgen Steffen Boch François Buscot Markus Fischer Kezia Goldmann Martin M.Gossner Falk Hänsel Kirsten Jung Peter Manning Thomas Nauss Yvonne Oelmann Rodica Pena Andrea Polle Swen C.Renner Michael Schloter Ingo Schöning Ernst-Detlef Schulze Emily F.Solly Elisabeth Sorkau Barbara Stempfhuber Tesfaye Wubet Jörg Müller Sebastian Seibold Wolfgang W.Weisser | 2021 | Forest Ecosystems2021，8，1： | 2 |
| 12 | Electrodeposited and selenized (CuInSe2) (CIS) thin films for photovoltaic applications显示文摘 | Fernandez A M Calixto M E Sebastian P J | 1998 | Solar Energy Materials &Solar Cells1998，52，34： | 2 |
| 13 | Gender-dependent association of CTLA4 polymorphisms with resolution of hepatitis C virus infection显示文摘 | Eckart Schott Heiko Witt Holger Hinrichsen Konrad Neumann Viola Weich Alexandra Bergk Juliane Halangk Tobias Müller Sebastian Tinjala Gero Puhl Peter Neuhaus Bertram Wiedenmann Thomas Berg | 2006 | Journal of Hepatology2006，，3： | 2 |
| 14 | 拉科酰胺在癫痫持续状态中的应用--针对现有证据的系统评价显示文摘拉科酰胺(Lacosamide,LCM)的静脉制剂具有良好的耐受性和安全性,促使其在癫痫持续状态(Status Epilepticus,SE)中得到应用。本项系统评价的目的是确定和评估LCM在SE中的使用情况。在电子数据库中用联合检索方式对2008年—2016年10月的文献进行了系统检索,并使用标准化的评估表格从各项研究中将有关研究设计、方法框架、数据源、有效性和LCM引起的不良反应的信息进行提取并系统地报告。研究共评估了522次SE发作,其中包括486例成人和36例儿童及青少年发作,女性占51.7%。LCM的总体效力为57%。LCM对非惊厥性(57%,82/145)和全面痉挛性(61%,30/49,P=0.68)SE的效力相似,但对局灶运动性SE的总体有效率更高(92%,34/39,P<0.001)。而LCM在用药次序靠后时其疗效从100%下降到20%。治疗期间的主要不良反应是眩晕,视力异常,复视和共济失调。总体而言,LCM具有良好的耐受性,并且没有具临床意义的药物相互作用。现有数据表明LCM在SE中的使用是有前景的,有效率为57%。LCM的优势在于没有潜在的药物相互作用,并且在紧急情况需迅速增加剂量时可静脉使用。 | Adam Strzelczyk Johann Philipp Zollner Laurent M Willems 肖英凤 童馨 慕洁 Julie Jost Esther Paule Susanne Schubert-Bast Felix Rosenow Sebastian Bauer | 2018 | 癫痫杂志2018，4，5： | 2 |
| 15 | Histone H3 lysine 4 monomethylation modulates long- range chromatin interactions at enhancers显示文摘在 enhancers 和倡导者之间的远程的染色质相互作用为在哺乳动物和另外的 metazoans 的许多发展地控制的基因的抄写是必要的。当前,把远侧的 enhancers 连接到他们的特定的目标倡导者的准确机制尚待充分被阐明。这里,我们证明 4 monomethylation (H3K4me1 )(MLL3/4 ) 和 histone methyltransferases MLL3 和 MLL4 玩的提高特定的 histone H3 离氨酸在这个过程的一个活跃角色。我们在区分老鼠表明那胚胎的干细胞,在 enhancers 的 H3K4me1 的 MLL3/4-dependent 免职与染色质相互作用的增加的层次相关,而这 histone 修正的损失在区别期间在基因激活导致染色质相互作用和缺点的减少的层次。H3K4me1 便于 Cohesin 建筑群的招募,染色质组织的一个已知的管理者到在 vitro 并且在 vivo 的染色质,提供潜在的机制让 MLL3/4 支持在 enhancers 和倡导者之间的染色质相互作用。一起拿,我们的结果在在哺乳动物的房间在 enhancers 安排远程的染色质相互作用为 MLL3/4-dependent H3K4me1 支持一个角色。 | Jian Yan Shi-An A Chen Andrea Local Tristin Liu Yunjiang Qiu Kristel M Dorighi Sebastian Preissl Chloe M Rivera Chaochen Wang Zhen Ye Kai Ge Ming Hu Joanna Wysocka Bing Ren | 2018 | Cell Research2018，28，2： | 2 |
| 16 | A prospective feasibility study of sub-millisievert abdominopelvic CT using iterative reconstruction in Crohn’s disease显示文摘 | Siobhan B O’Neill Patrick D Mc Laughlin Lee Crush Owen J O’Connor Sebastian R Mc Williams Orla Craig Anne Marie Mc Garrigle Fiona O’Neill Jackie Bye Max F. Ryan Fergus Shanahan Michael M Maher | 2013 | European Radiology2013，，： | 2 |
| 17 | Significance of an additional unenhanced scan in computed tomography angiography of patients with suspected acute aortic syndrome显示文摘AIM To assess potential benefits of an additional unenhanced acquisition in computed tomography angiography(CTA) in patients with suspected acute aortic syndrome(AAS).METHODS A total of 103 aortic CTA(non-electrocardiography-gated, 128 slices) performed due to suspected AAS were retrospectively evaluated for acute aortic dissection(AAD), intramural hematoma(IMH), or penetrating aortic ulcer(PAU). Spiral CTA protocol consisted of an unenhanced acquisition and an arterial phase. If AAS was detected, a venous phase(delay, 90 s) was added. Images were evaluated for the presence and extent of AAD, IMH, PAU, and related complications. The diagnostic benefit of the unenhanced acquisition was evaluated concerning detection of IMH.RESULTS Fifty-six(30% women; mean age, 67 years; median, 68 years) of the screened individuals had AAD or IMH. A triphasic CT scan was conducted in 76.8%(n =43). 56% of the detected AAD were classified as Stanford type A, 44% as Stanford type B. 53.8% of the detected IMH were classified as Stanford type A, 46.2% as Stanford type B. There was no significant difference in the involvement of the ascending aorta between AAD and IMH(P = 1.0) or in the average age between AAD and IMH(P = 0.548), between Stanford type A and Stanford type B in general(P = 0.650) and between Stanford type A and Stanford type B within the entities of AAD and IMH(AAD: P = 0.785; IMH: P = 0.146). Only the unenhanced acquisitions showed a significant density difference between the adjacent lumen and the IMH(P = 0.035). Subadventitial hematoma involving the pulmonary trunk was present in 5 patients(16%) with Stanford A AAD. The difference between the median radiation exposure of a triphasic(2737 mGy*cm) compared to a biphasic CT scan(2135 mGy*cm) was not significant(P = 0.135).CONCLUSION IMH is a common and difficult to detect entity of AAS. An additional unenhanced acquisition within an aortic CTA protocol facilitates the detection of IMH. | Nikolaos Panagiotopoulos Felix Drüschler Martin Simon Florian M Vogt Sebastian Wolfrum Steffen Desch Doreen Richardt Jorg Barkhausen Peter Hunold | 2018 | World Journal of Radiology2018，10，11： | 2 |
| 18 | Adsorptive separation of light olefins from paraffins 显示文摘 | Douglas M Ruthven Sebastian C Reyes | 2007 | Microporous and Mesoporous Materials2007，104，： | 1 |
| 19 | Nonlinear FE analysis of steel-concrete composite structures 显示文摘 | Sebastian W M McConnel R E | 2000 | Journal of Structural Engineering2000，126，6： | 1 |
| 20 | Plasticity model for predictingpunching shear strengths of reinforced concrete slabs 显示文摘 | Salim W Sebastian W M | 2002 | ACI Structural Journal2002，99，6： | 1 |
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